LOCUS       Exported                9120 bp ds-DNA     circular SYN 26-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             296..1237
                     /codon_start=1
                     /label=Spectinomycin adenyltransferase
                     /label=Spectinomycin adenyltransferase
                     /translation="MACYDCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPK
                     TKLNIMGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTV
                     TVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
                     RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLTL
                     WNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQAY
                     LGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             446..1237
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     Replication_ori complement(1546..4331)
                     /direction=LEFT
                     /label=pVS1ori short
                     /note="/vntifkey=21"
     rep_origin      1748..1942
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2008..3081)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3510..4139)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4403..4440
                     /label=Left border
                     /label=nonstandard type: Region
     misc_feature    4403..4427
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4995..5340
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5350..5376
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5380..5406
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5410..5436
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5443..6399
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             complement(6453..6758)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(7100..7759)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(7813..7843)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     terminator      8027..8279
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(8340..8384)
                     /label=Right border
                     /label=nonstandard type: Region
     misc_feature    8340..8364
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(8487..9075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     Replication_ori 8487
                     /label=ORI
                     /note=" RNaseH cleavage point "
                     /note="/vntifkey=33"
     Replication_ori complement(8591..8871)
                     /direction=LEFT
                     /label=pBR322 origin of replication
ORIGIN
        1 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
       61 atcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
      121 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
      181 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
      241 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
      301 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
      361 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
      421 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
      481 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      541 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      601 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      661 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      721 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      781 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      841 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      901 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      961 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     1021 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     1081 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     1141 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     1201 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
     1261 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     1321 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     1381 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     1441 ccactgagcg tcagaccccg tagaaaagat caaaggatct tctgccattt ttggggtgag
     1501 gccgttcgcg gccgaggggc gcagcccctg gggggatggg agctcggtgc cttgatgtgg
     1561 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
     1621 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
     1681 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
     1741 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
     1801 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
     1861 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
     1921 cccaatgtac gtgctatcca caggaaagaa gctttttcga cctttttccc ctgctagggc
     1981 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
     2041 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
     2101 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
     2161 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
     2221 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
     2281 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
     2341 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
     2401 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
     2461 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
     2521 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
     2581 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
     2641 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
     2701 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
     2761 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
     2821 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
     2881 aatcgacgac gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
     2941 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
     3001 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
     3061 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
     3121 ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
     3181 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
     3241 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
     3301 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
     3361 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
     3421 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
     3481 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
     3541 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
     3601 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
     3661 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
     3721 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
     3781 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
     3841 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
     3901 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
     3961 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
     4021 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
     4081 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
     4141 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
     4201 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
     4261 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
     4321 gcagccgcac ggctgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     4381 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
     4441 gacacttaat aacacattgc ggacgttttt aatgtactgg ggtggattac ggaagacaat
     4501 acggcggaga ttagcctttt caatttcaga aagaatgcta acccacagat ggttagagag
     4561 gcttacgcag cacgactcat caagacgatc tacccgagca ataatctcca ggaaatcaaa
     4621 taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac
     4681 acagagaaag atatatttct caagatcaga agtactattc cagtatggac gattcaaggc
     4741 ttgcttcaca aaccaaggca agtaatagag attggagtct ctaaaaaggt agttcccact
     4801 gaatcaaagg ccatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag
     4861 actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa aatcttcgtc
     4921 aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa
     4981 gagcaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc
     5041 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac
     5101 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
     5161 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaacacgt tccaaccacg
     5221 acttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     5281 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca
     5341 cgtctgatgt acccgtatga tgttccggat tacgctggct atccctacga cgtgcccgac
     5401 tatgccgggt acccctatga cgtcccagac tacgcagcta gcaaagacaa tactgtgcct
     5461 ctgaagctga tcgctctcct ggctaatggc gagttccata gtggcgaaca gctgggagaa
     5521 accctgggca tgtccagggc cgctatcaac aagcacattc agactctgcg cgactggggc
     5581 gtggacgtgt tcaccgtgcc cggaaagggc tactctctgc ccgagcctat cccgctgctg
     5641 aacgctaaac agattctggg acagctggac ggcgggagcg tggcagtcct gcctgtggta
     5701 gactccacca atcagtacct gctggatcga atcggcgagc tgaagagtgg ggatgcttgc
     5761 attgcagaat atcagcaggc agggagagga agcagaggga ggaaatggtt ctctcctttt
     5821 ggagctaacc tgtacctgag tatgttttgg cgcctgaagc ggggaccagc agcaatcggc
     5881 ctgggcccgg tcatcggaat tgtcatggca gaagcgctgc gaaagctggg agcagacaag
     5941 gtgcgagtca aatggcccaa tgacctgtat ctgcaggata gaaagctggc aggcatcctg
     6001 gtggagctgg ccggaataac aggcgatgct gcacagatcg tcattggcgc cgggattaac
     6061 gtggctatga ggcgcgtgga ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa
     6121 gcagggatta acctggacag gaatactctg gccgctacgc tgatccgaga gctgcgggca
     6181 gccctggaac tgttcgagca ggaaggcctg gctccatatc tgccacggtg ggagaagctg
     6241 gataacttca tcaatagacc cgtgaagctg atcattgggg acaaagagat tttcgggatt
     6301 agccggggga ttgataaaca gggagccctg ctgctggaac aggacggagt tatcaaaccc
     6361 tggatgggcg gagaaatcag tctgcggtct gccgaaaagc tcacctgaga cggtcgacct
     6421 gcagactggc tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg
     6481 gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggaa
     6541 accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc
     6601 accgggtaaa gttcacgggg gactttatct gacagcagac gtgcactggc cagggggatc
     6661 accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa
     6721 cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc
     6781 agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc
     6841 cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag
     6901 accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc
     6961 actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag
     7021 tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggctttta
     7081 gtaagccgga tcctctagat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     7141 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     7201 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     7261 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     7321 ctgacacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     7381 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     7441 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     7501 tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca
     7561 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     7621 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     7681 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     7741 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgac ggatcctaac
     7801 tcaaaatcca cacattatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7861 cggcccctcg aggggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
     7921 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     7981 ttcagctttc gtctctaagg gctaagacct aagctagcct agaatcgatc gttcaaacat
     8041 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     8101 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     8161 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     8221 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatct
     8281 gagtgaccta agctagccta gtgtcgggct tgtcttctct ggtttaaact atcagtgttt
     8341 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     8401 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgccaa ccacaggaac
     8461 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8521 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8581 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8641 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8701 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8761 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8821 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8881 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8941 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9001 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9061 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
//
