LOCUS       Exported                8460 bp ds-DNA     circular SYN 04-AUG-2023
DEFINITION  Golden Gate ligation product.
ACCESSION   .
VERSION     .
KEYWORDS    10.pGTT2-35S-sGFP-Lti6b-TurboID-nosT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8460
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             296..1237
                     /label=Spectinomycin adenyltransferase
     misc_feature    complement(1546..4331)
                     /label=pVS1ori short
                     /note="Geneious type: Replication_ori"
                     /note="/vntifkey=21"
     misc_feature    4403..4440
                     /label=Left border
                     /note="Geneious type: Region"
     misc_feature    4504..5346
                     /label=LI_A-B_35S_(G5) (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    4504..4507
                     /label=Ligation
                     /note="Geneious type: ligation"
     promoter        4508..5342
                     /label=35S Promoter
     misc_feature    5343..6118
                     /label=LI_B-C_GFP_(G28) (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    5343..5346
                     /label=Ligation
                     /note="Geneious type: ligation"
     CDS             5351..6063
                     /codon_start=1
                     /transl_table=8
                     /gene="sGFP"
                     /product="sGFP"
                     /label=sGFP CDS
                     /note="sGFP (S65T) gene"
                     /db_xref="GI:170172452"
                     /protein_id="BAG12994.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDEL"
     CDS             6068..6112
                     /label=15 aa linker
     misc_feature    6115..7340
                     /label=CD-Lti6b-TurboID-Stop (concatenated sequence 4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    6115..6118
                     /label=Ligation
                     /note="Geneious type: ligation"
     misc_feature    7337..7366
                     /label=LI_dy_D-E_-_BB8 (concatenated sequence 5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    7337..7340
                     /label=Ligation
                     /note="Geneious type: ligation"
     misc_feature    7341..7362
                     /label=LI D-E dummie
                     /note="Geneious type: Misc."
     misc_feature    7345..7362
                     /label=stops in all frames
                     /note="Geneious type: Misc._feature"
     misc_feature    7363..7623
                     /label=LI_E-F_nos-T (concatenated sequence 6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    7363..7366
                     /label=Ligation
                     /note="Geneious type: ligation"
     terminator      7367..7619
                     /label=Nos Terminator
     misc_feature    7620..7645
                     /label=LI_dy_F-G_-_BB9 (concatenated sequence 7)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    7620..7623
                     /label=Ligation
                     /note="Geneious type: ligation"
     misc_feature    7624..7641
                     /label=stops in all frames
                     /note="Geneious type: Misc._feature"
     misc_feature    7624..7641
                     /label=LI F-G dummie
                     /note="Geneious type: Misc."
     misc_feature    7642..12967
                     /label=LIIa_F_1-2_-_BB10 (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    7642..7645
                     /label=Ligation
                     /note="Geneious type: ligation"
     misc_feature    complement(7680..7724)
                     /label=Right border
                     /note="Geneious type: Region"
     misc_feature    7827
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="Geneious type: Replication_ori"
                     /note="/vntifkey=33"
     misc_feature    complement(7931..8211)
                     /label=pBR322 origin of replication
                     /note="Geneious type: Replication_ori"
ORIGIN
        1 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
       61 atcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
      121 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
      181 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
      241 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
      301 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
      361 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
      421 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
      481 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      541 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      601 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      661 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      721 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      781 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      841 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      901 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      961 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     1021 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     1081 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     1141 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     1201 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
     1261 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     1321 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     1381 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     1441 ccactgagcg tcagaccccg tagaaaagat caaaggatct tctgccattt ttggggtgag
     1501 gccgttcgcg gccgaggggc gcagcccctg gggggatggg agctcggtgc cttgatgtgg
     1561 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
     1621 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
     1681 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
     1741 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
     1801 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
     1861 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
     1921 cccaatgtac gtgctatcca caggaaagaa gctttttcga cctttttccc ctgctagggc
     1981 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
     2041 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
     2101 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
     2161 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
     2221 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
     2281 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
     2341 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
     2401 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
     2461 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
     2521 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
     2581 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
     2641 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
     2701 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
     2761 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
     2821 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
     2881 aatcgacgac gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
     2941 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
     3001 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
     3061 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
     3121 ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
     3181 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
     3241 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
     3301 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
     3361 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
     3421 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
     3481 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
     3541 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
     3601 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
     3661 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
     3721 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
     3781 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
     3841 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
     3901 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
     3961 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
     4021 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
     4081 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
     4141 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
     4201 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
     4261 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
     4321 gcagccgcac ggctgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     4381 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
     4441 gacacttaat aacacattgc ggacgttttt aatgtactgg ggtggattac ggaagacaat
     4501 acggcggaga ttagcctttt caatttcaga aagaatgcta acccacagat ggttagagag
     4561 gcttacgcag cacgactcat caagacgatc tacccgagca ataatctcca ggaaatcaaa
     4621 taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac
     4681 acagagaaag atatatttct caagatcaga agtactattc cagtatggac gattcaaggc
     4741 ttgcttcaca aaccaaggca agtaatagag attggagtct ctaaaaaggt agttcccact
     4801 gaatcaaagg ccatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag
     4861 actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa aatcttcgtc
     4921 aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa
     4981 gagcaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc
     5041 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac
     5101 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
     5161 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaacacgt tccaaccacg
     5221 acttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     5281 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca
     5341 cgtctgaaca atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     5401 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     5461 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     5521 ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc gctaccccga
     5581 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     5641 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     5701 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     5761 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     5821 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     5881 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     5941 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     6001 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct
     6061 gtacaagggt ggaggaggtt ctggaggcgg tggaagtggt ggcggaggta gcggcaccat
     6121 gagtacagcc actttcgtag agattattct tgctatcatc ttgcctcctc tcggcgtctt
     6181 tctcaaattt ggttgcaagg ttgagttttg gatatgtttg attttgacgc tgtttggtta
     6241 tcttcccgga atcctttacg ctctttatat catcaccaag atgtacccgt atgatgttcc
     6301 ggattacgct ggctatccct acgacgtgcc cgactatgcc gggtacccct atgacgtccc
     6361 agactacgca gctagcaaag acaatactgt gcctctgaag ctgatcgctc tcctggctaa
     6421 tggcgagttc catagtggcg aacagctggg agaaaccctg ggcatgtcca gggccgctat
     6481 caacaagcac attcagactc tgcgcgactg gggcgtggac gtgttcaccg tgcccggaaa
     6541 gggctactct ctgcccgagc ctatcccgct gctgaacgct aaacagattc tgggacagct
     6601 ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt acctgctgga
     6661 tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc aggcagggag
     6721 aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc tgagtatgtt
     6781 ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg gaattgtcat
     6841 ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc ccaatgacct
     6901 gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa taacaggcga
     6961 tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag
     7021 cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac
     7081 tctggccgct acgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg
     7141 cctggctcca tatctgccac ggtgggagaa gctggataac ttcatcaata gacccgtgaa
     7201 gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc
     7261 cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg
     7321 gtctgccgaa aagtagaagg gctaagacct aagctagcct agaatcgatc gttcaaacat
     7381 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     7441 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     7501 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     7561 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatct
     7621 gagtgaccta agctagccta gtgtcgggct tgtcttctct ggtttaaact atcagtgttt
     7681 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     7741 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgccaa ccacaggaac
     7801 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7861 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7921 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7981 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8041 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8101 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8161 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8221 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8281 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8341 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8401 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
//
