LOCUS       Exported                8505 bp ds-DNA     circular SYN 04-AUG-2023
DEFINITION  Golden Gate ligation product.
ACCESSION   .
VERSION     .
KEYWORDS    8.pGTT2-35S-RbcS1a-PTP-TurboID-sGFP-nosT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 19, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             296..1237
                     /label=Spectinomycin adenyltransferase
     misc_feature    complement(1546..4331)
                     /label=pVS1ori short
                     /note="Geneious type: Replication_ori"
                     /note="/vntifkey=21"
     misc_feature    4403..4440
                     /label=Left border
                     /note="Geneious type: Region"
     misc_feature    4504..5346
                     /label=LI_A-C_35S_(G9) (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    4504..4507
                     /label=Ligation
                     /note="Geneious type: ligation"
     promoter        4508..5342
                     /label=35S Promoter
     misc_feature    5343..6643
                     /label=CD-RbcS1a-PTP-TurboID-NS (concatenated sequence 3)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    5343..5346
                     /label=Ligation
                     /note="Geneious type: ligation"
     misc_feature    6640..7411
                     /label=LI_D-E_GFP_(G11) (concatenated sequence 4)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    6640..6643
                     /label=Ligation
                     /note="Geneious type: ligation"
     CDS             6646..6690
                     /label=15 aa linker
     CDS             6691..7407
                     /codon_start=1
                     /transl_table=8
                     /gene="sGFP"
                     /product="sGFP"
                     /label=sGFP CDS
                     /note="sGFP (S65T) gene"
                     /db_xref="GI:170172452"
                     /protein_id="BAG12994.1"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    7408..7668
                     /label=LI_E-F_nos-T (concatenated sequence 5)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    7408..7411
                     /label=Ligation
                     /note="Geneious type: ligation"
     terminator      7412..7664
                     /label=Nos Terminator
     misc_feature    7665..7690
                     /label=LI_dy_F-G_-_BB9 (concatenated sequence 6)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    7665..7668
                     /label=Ligation
                     /note="Geneious type: ligation"
     misc_feature    7669..7686
                     /label=LI F-G dummie
                     /note="Geneious type: Misc."
     misc_feature    7669..7686
                     /label=stops in all frames
                     /note="Geneious type: Misc._feature"
     misc_feature    7687..13012
                     /label=LIIa_F_1-2_-_BB10 (concatenated sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source"
     misc_feature    7687..7690
                     /label=Ligation
                     /note="Geneious type: ligation"
     misc_feature    complement(7725..7769)
                     /label=Right border
                     /note="Geneious type: Region"
     misc_feature    7872
                     /label=ORI
                     /note="RNaseH cleavage point"
                     /note="Geneious type: Replication_ori"
                     /note="/vntifkey=33"
     misc_feature    complement(7976..8256)
                     /label=pBR322 origin of replication
                     /note="Geneious type: Replication_ori"
ORIGIN
        1 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
       61 atcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
      121 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca
      181 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
      241 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
      301 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
      361 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
      421 tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca
      481 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      541 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      601 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      661 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      721 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      781 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      841 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      901 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      961 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     1021 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     1081 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     1141 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     1201 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa
     1261 gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg
     1321 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     1381 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     1441 ccactgagcg tcagaccccg tagaaaagat caaaggatct tctgccattt ttggggtgag
     1501 gccgttcgcg gccgaggggc gcagcccctg gggggatggg agctcggtgc cttgatgtgg
     1561 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
     1621 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
     1681 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
     1741 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
     1801 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
     1861 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
     1921 cccaatgtac gtgctatcca caggaaagaa gctttttcga cctttttccc ctgctagggc
     1981 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
     2041 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
     2101 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
     2161 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
     2221 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
     2281 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
     2341 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
     2401 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
     2461 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
     2521 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
     2581 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
     2641 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
     2701 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
     2761 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
     2821 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
     2881 aatcgacgac gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
     2941 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
     3001 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
     3061 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
     3121 ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
     3181 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
     3241 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
     3301 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
     3361 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
     3421 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
     3481 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
     3541 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
     3601 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
     3661 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
     3721 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
     3781 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
     3841 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
     3901 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
     3961 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
     4021 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
     4081 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
     4141 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
     4201 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
     4261 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
     4321 gcagccgcac ggctgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     4381 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
     4441 gacacttaat aacacattgc ggacgttttt aatgtactgg ggtggattac ggaagacaat
     4501 acggcggaga ttagcctttt caatttcaga aagaatgcta acccacagat ggttagagag
     4561 gcttacgcag cacgactcat caagacgatc tacccgagca ataatctcca ggaaatcaaa
     4621 taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac
     4681 acagagaaag atatatttct caagatcaga agtactattc cagtatggac gattcaaggc
     4741 ttgcttcaca aaccaaggca agtaatagag attggagtct ctaaaaaggt agttcccact
     4801 gaatcaaagg ccatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag
     4861 actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa aatcttcgtc
     4921 aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa
     4981 gagcaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc
     5041 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac
     5101 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
     5161 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaacacgt tccaaccacg
     5221 acttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     5281 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca
     5341 cgcaccatgg cttcctctat gctctcttcc gctactatgg ttgcctctcc ggctcaggcc
     5401 actatggtcg ctcctttcaa cggacttaag tcctccgctg ccttcccagc cacccgcaag
     5461 gctaacaacg acattacttc catcacaagc aacggcggaa gagttaactg catgcaggtg
     5521 tggcctccga ttggaaagaa gaagtttgag actctctctt accttcctga ccttaccgat
     5581 tccgaaatgt acccgtatga tgttccggat tacgctggct atccctacga cgtgcccgac
     5641 tatgccgggt acccctatga cgtcccagac tacgcagcta gcaaagacaa tactgtgcct
     5701 ctgaagctga tcgctctcct ggctaatggc gagttccata gtggcgaaca gctgggagaa
     5761 accctgggca tgtccagggc cgctatcaac aagcacattc agactctgcg cgactggggc
     5821 gtggacgtgt tcaccgtgcc cggaaagggc tactctctgc ccgagcctat cccgctgctg
     5881 aacgctaaac agattctggg acagctggac ggcgggagcg tggcagtcct gcctgtggtc
     5941 gactccacca atcagtacct gctggatcga atcggcgagc tgaagagtgg ggatgcttgc
     6001 attgcagaat atcagcaggc agggagagga agcagaggga ggaaatggtt ctctcctttt
     6061 ggagctaacc tgtacctgag tatgttttgg cgcctgaagc ggggaccagc agcaatcggc
     6121 ctgggcccgg tcatcggaat tgtcatggca gaagcgctgc gaaagctggg agcagacaag
     6181 gtgcgagtca aatggcccaa tgacctgtat ctgcaggata gaaagctggc aggcatcctg
     6241 gtggagctgg ccggaataac aggcgatgct gcacagatcg tcattggcgc cgggattaac
     6301 gtggctatga ggcgcgtgga ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa
     6361 gcagggatta acctggacag gaatactctg gccgctacgc tgatccgaga gctgcgggca
     6421 gccctggaac tgttcgagca ggaaggcctg gctccatatc tgccacggtg ggagaagctg
     6481 gataacttca tcaatagacc cgtgaagctg atcattgggg acaaagagat tttcgggatt
     6541 agccggggga ttgataaaca gggagccctg ctgctggaac aggacggagt tatcaaaccc
     6601 tggatgggcg gagaaatcag tctgcggtct gccgaaaaga agggaggtgg aggaggttct
     6661 ggaggcggtg gaagtggtgg cggaggtagc gtgagcaagg gcgaggagct gttcaccggg
     6721 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     6781 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     6841 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg cgtgcagtgc
     6901 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     6961 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     7021 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     7081 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     7141 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     7201 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     7261 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     7321 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     7381 cacggcatgg acgagctgta caagtaaaat cgatcgttca aacatttggc aataaagttt
     7441 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     7501 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     7561 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     7621 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatctgagtg acctaagcta
     7681 gcctagtgtc gggcttgtct tctctggttt aaactatcag tgtttgacag gatatattgg
     7741 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
     7801 aaaggtttat ccgttcgtcc atttgtatgt gccaaccaca ggaaccgtaa aaaggccgcg
     7861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8281 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaac
//
