LOCUS       Exported               12630 bp DNA     circular SYN 19-SEP-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2023 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..6165
                     /codon_start=1
                     /gene="hsSCN8A (Nav 1.6)"
                     /label=hsSCN8A (Nav 1.6)
                     /note="hsSCN8A (Nav 1.6)"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=33023"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=2"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #993366"
                     /translation="MAARLLAPPGPDSFKPFTPESLANIERRIAESKLKKPPKADGSHR
                     EDDEDSKPKPNSDLEAGKSLPFIYGDIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKTLF
                     RFSATPALYILSPFNLIRRIAIKILIHSVFSMIIMCTILTNCVFMTFSNPPDWSKNVEY
                     TFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVDLGNVSALRTF
                     RVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKC
                     VVWPINFNESYLENGTKGFDWEEYINNKTNFYTVPGMLEPLLCGNSSDAGQCPEGYQCM
                     KAGRNPNYGYTSFDTFSWAFLALFRLMTQDYWENLYQLTLRAAGKTYMIFFVLVIFVGS
                     FYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFKAMLEQLKKQQEEAQAAAMATSAGTV
                     SEDAIEEEGEEGGGSPRSSSEISKLSSKSAKERRNRRKKRKQKELSEGEEKGDPEKVFK
                     SESEDGMRRKAFRLPDNRIGRKFSIMNQSLLSIPGSPFLSRHNSKSSIFSFRGPGRFRD
                     PGSENEFADDEHSTVEESEGRRDSLFIPIRARERRSSYSGYSGYSQGSRSSRIFPSLRR
                     SVKRNSTVDCNGVVSLIGGPGSHIGGRLLPEATTEVEIKKKGPGSLLVSMDQLASYGRK
                     DRINSIMSVVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKLKEIVNLIVMDP
                     FVDLAITICIVLNTLFMAMEHHPMTPQFEHVLAVGNLVSMKFRDTAYLPNSGNVKY*QN
                     FSLFPSHRFSLEFSQRKCS*SS*PWIPTIISKKVGTFLTDLLSPSV*WN*V*QTWRGFQ
                     CCDLSDCSESSNWPNPGPP*TC*SRLLEIQWVPWAT*HWCWPLLSSSLPWWGCNSLEKA
                     TKSVSARSTRTVNSLAGICMTFSIPSSLSFECCAGSGLRPCGTAWKWQARPCASLSL*W
                     SW*LATWWC*TCFWPCS*APSVQTTWLPQMTMGK*TTSRSQ*SVSRRVWPGPN*RCTPS
                     CRPTLSSVRLMR*SLWMSCMKRRPTVSPITPVQTSTGMVTSRRMAMAQPAALAAAWRST
                     SLMRTTCPSSTTPT*LYGYPLLWASLTLRTSTQRMLAASRILKAAKIN*MTPAPLKEAP
                     LISNQK*KRSLWNSLRNTWIQMPASQKVVSSGSSAARSTSRKG*ASLGGSCGKPASSSW
                     STTGLRPSSSS*FC*AVAPWPSRTSTLSRERPSAPSWNMLTKSSPISSSWRCCSSGQPM
                     ASSSSSPMPGVGWTSSLWLSL*SAL*LMPWATRN*VP*SPLGP*EL*DP*EPYHDLKG*
                     GWW*MPWWAPSPPS*MCCWCVSSSG*FSASWELTCLRESTTTALMRLLKSDLKLKMSTI
                     RPNVKSLWRGTIQRSDGRT*RSTLTMLGQDTWPFFK*QPSKAGWTSCMQL*IPGR*VSR
                     LQDRFKETNRNWACRDREDSCVSDRHLLVLLTSTLPFSPQPDEQPKYEDNIYMYIYFVI
                     FIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIP
                     RPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVETDTQSKQMENILYWINLVFVIF
                     FTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVSPTLFRVIRLAR
                     IGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKHEAGI
                     DDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHPGSGFKGDCGNPSV
                     GIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDAT
                     QFIEYCKLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGDS
                     GELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAVVLQRAYRGHLARRGFICK
                     KTTSNKLENGGTHREKKESTPSTASLPSYDSVTKPEKEKQQRAEEGRRERAKRQKEVRE
                     SKC"
     misc_feature    615..702
                     /gene="Adult Exon"
                     /label=Adult Exon
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=16744703"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1.07"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    2371..2459
                     /gene="HSPA5_Int4_v2"
                     /label=HSPA5_Int4_v2
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=12632256"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1.07"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    4371..4503
                     /gene="IVS"
                     /label=IVS
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=12632256"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1.07"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    6190..6779
                     /gene="IRES2 (A6) Variant"
                     /label=IRES2 (A6) Variant
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=32896"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    6777..7472
                     /gene="mScarlet"
                     /label=mScarlet
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=255"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=2"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    7526..7555
                     /gene="Myc Tag"
                     /label=Myc Tag
                     /note="Myc Tag"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=12632319"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    7571..7588
                     /gene="6x His Tag"
                     /label=6x His Tag
                     /note="6x His Tag"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=16744703"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    7601..7824
                     /gene="BGH polyA"
                     /label=BGH polyA
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8421631"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    7904..8210
                     /gene="f1_origin"
                     /label=f1_origin
                     /note="f1_origin"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=12632256"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    8509..8586
                     /gene="SV40_origin"
                     /label=SV40_origin
                     /note="SV40_origin"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=12448559"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    8772..9143
                     /gene="ZeoR"
                     /label=ZeoR
                     /note="ZeoR"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8078638"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=2"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     polyA_signal    9279..9397
                     /gene="SV40 Early PolyA Terminator"
                     /label=SV40 Early PolyA Terminator
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=255"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=6"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     protein_bind    complement(9468..9490)
                     /gene="LacO"
                     /label=LacO
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=10280598"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=6"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #31849b; direction: LEFT"
     promoter        complement(9495..9524)
                     /gene="LacZ Promoter"
                     /label=LacZ Promoter
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454143"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=6"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    complement(9539..9560)
                     /gene="CAP binding site"
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence 
                     of cAMP"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8128968"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=2"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    complement(9846..10437)
                     /gene="pBR322-Derived Ori"
                     /label=pBR322-Derived Ori
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=12632256"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    9848..10436
                     /gene="Ori"
                     /label=Ori
                     /note="Ori"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=12632256"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    complement(9861..9910)
                     /gene="Hairpin 1"
                     /label=Hairpin 1
                     /note="pUC Rep Ori Hairpin 1"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=2"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    10043..10052
                     /gene="New Feature"
                     /label=New Feature
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_codon_table=Standard"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=0"
                     /note="/pLOT_fWidth=5"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     promoter        10254..10283
                     /gene="pBR322 Ori RNAI Promoter"
                     /label=pBR322 Ori RNAI Promoter
                     /note="pUC Rep Ori RNAI Promoter"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454143"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=6"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #ffffff"
     misc_feature    10281..10397
                     /gene="pBR322 Ori RNAI transcript"
                     /label=pBR322 Ori RNAI transcript
                     /note="pBR322 Ori RNAI transcript"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=2"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    10289..10300
                     /gene="New Feature"
                     /label=New Feature
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_codon_table=Standard"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=0"
                     /note="/pLOT_fWidth=5"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    complement(10299..10328)
                     /gene="Loop 3"
                     /label=Loop 3
                     /note="pUC Rep Ori Loop 3.  Initiates RNAI/RNAII 
                     interaction."
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=255"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=2"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(10331..10359)
                     /gene="Loop 2"
                     /label=Loop 2
                     /note="pUC Rep Ori Loop 2.  Initiates RNAI/RNAII 
                     interaction."
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=255"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=2"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(10362..10394)
                     /gene="Loop 1"
                     /label=Loop 1
                     /note="pUC Rep Ori Loop 1.  Initiates RNAI/RNAII 
                     interaction."
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=255"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=2"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3; direction: LEFT"
     misc            10369..10394
                     /gene="pBR322 Ori RNAI Terminator"
                     /label=pBR322 Ori RNAI Terminator
                     /note="pUC Rep Ori RNAI transcription terminator"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=3"
                     /note="/pLOT_fWidth=2"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc            complement(10408..10436)
                     /direction=LEFT
                     /gene="pBR322 Ori RNAII Promoter"
                     /label=pBR322 Ori RNAII Promoter
                     /note="pUC Rep Ori RNAII Promoter"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454016"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=6"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    complement(10607..11464)
                     /gene="Amp ORF"
                     /label=Amp ORF
                     /note="Amp ORF"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=65535"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=2"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(10607..11266)
                     /codon_start=1
                     /gene="B-Lactamase"
                     /label=B-Lactamase
                     /note="Confers resistance to ampicillin"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=65535"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=2"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #993366"
                     /translation="STFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLT
                     DGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAI
                     PNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIA
                     DKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             complement(10607..11266)
                     /codon_start=1
                     /gene="Amp ORF"
                     /label=Amp ORF
                     /note="Confers resistance to ampicillin"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=65535"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=2"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #993366"
                     /translation="STFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLT
                     DGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAI
                     PNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIA
                     DKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     misc_feature    complement(11264..11464)
                     /gene="B-lactamase Secretion Signal"
                     /label=B-lactamase Secretion Signal
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454143"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(11509..11537)
                     /gene="AmpR Promoter"
                     /label=AmpR Promoter
                     /note="AmpR Promoter"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454143"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=-1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    11863..12415
                     /gene="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
                     /note="CMV_immearly_promoter"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8622608"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    12257
                     /gene="-179 CpG"
                     /label=-179 CpG
                     /note="-179 CpG (cgtggatagCG) C>G confers resistance to 
                     methylation and higher expression in CHO cells."
                     /note="CMV promoter mutants with a reduced propensity to 
                     productivity loss in CHO cells"
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=0"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=0"
                     /note="/pLOT_fWidth=4"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #a6acb3"
     misc_feature    12423..12441
                     /gene="TetO"
                     /label=TetO
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454143"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #31849b"
     misc_feature    12444..12462
                     /gene="TetO"
                     /label=TetO
                     /note="/pLOT_Alpha=255"
                     /note="/pLOT_Color=8454016"
                     /note="/pLOT_FHide=0"
                     /note="/pLOT_ShowLabel=1"
                     /note="/pLOT_fOrient=1"
                     /note="/pLOT_fType=1"
                     /note="/pLOT_fWidth=0.3"
                     /note="/pLOT_rOffset=1"
                     /note="/pLOT_tcolor=0"
                     /note="color: #31849b"
ORIGIN
        1 atggcagcgc ggctgcttgc accaccaggc cctgatagtt tcaagccttt cacccctgag
       61 tcactggcaa acattgagag gcgcattgct gagagcaagc tcaagaaacc accaaaggcc
      121 gatggcagtc atcgggagga cgatgaggac agcaagccca agccaaacag cgacctggaa
      181 gcagggaaga gtttgccttt catctacggg gacatccccc aaggcctggt tgcagttccc
      241 ctggaggact ttgacccata ctatttgacg cagaaaacct ttgtagtatt aaacagaggg
      301 aaaactctct tcagatttag tgccacgcct gccttgtaca ttttaagtcc ttttaacctg
      361 ataagaagaa tagctattaa aattttgata cattcagtat ttagcatgat cattatgtgc
      421 actattttga ccaactgtgt attcatgact tttagtaacc ctcctgactg gtcgaagaat
      481 gtggagtaca cgttcacagg gatttataca tttgaatcac tagtgaaaat cattgcaaga
      541 ggtttctgca tagatggctt taccttttta cgggacccat ggaactggtt agatttcagt
      601 gtcatcatga tggcatatgt gacagagttt gtggacctgg gcaatgtctc agcgctgaga
      661 acattcaggg ttctccgagc tttgaaaact atctctgtaa ttccaggcct gaagacaatt
      721 gtgggtgccc tgattcagtc tgtgaagaaa ctgtcagatg tgatgatcct gacagtgttc
      781 tgcctgagtg tttttgcctt gatcggactg cagctgttca tggggaacct tcgaaacaag
      841 tgtgttgtgt ggcccataaa cttcaacgag agctatcttg aaaatggcac caaaggcttt
      901 gattgggaag agtatatcaa caataaaaca aatttctaca cagttcctgg catgctggaa
      961 cctttactct gtgggaacag ttctgatgct gggcaatgcc cagagggata ccagtgtatg
     1021 aaagcaggaa ggaaccccaa ctatggttac acaagttttg acacttttag ctgggccttc
     1081 ttggcattat ttcgccttat gacccaggac tattgggaaa acttgtatca attgacttta
     1141 cgagcagccg ggaaaacata catgatcttc ttcgtcttgg tcatctttgt gggttctttc
     1201 tatctggtga acttgatctt ggctgtggtg gccatggctt atgaagaaca gaatcaggca
     1261 acactggagg aggcagaaca aaaagaggct gaatttaaag caatgttgga gcaacttaag
     1321 aagcaacagg aagaggcaca ggctgctgcg atggccactt cagcaggaac tgtctcagaa
     1381 gatgccatag aggaagaagg tgaagaagga gggggctccc ctcggagctc ttctgaaatc
     1441 tctaaactca gctcaaagag tgcaaaggaa agacgtaaca ggagaaagaa gaggaagcaa
     1501 aaggaactct ctgaaggaga ggagaaaggg gatcccgaga aggtgtttaa gtcagagtca
     1561 gaagatggca tgagaaggaa ggcctttcgg ctgccagaca acagaatagg gaggaaattt
     1621 tccatcatga atcagtcact gctcagcatc ccaggctcgc ccttcctctc ccgccacaac
     1681 agcaagagca gcatcttcag tttcagggga cctgggcggt tccgagaccc gggctccgag
     1741 aatgagttcg cggatgacga gcacagcacg gtggaggaga gcgagggccg ccgggactcc
     1801 ctcttcatcc ccatccgggc ccgcgagcgc cggagcagct acagcggcta cagcggctac
     1861 agccagggca gccgctcctc gcgcatcttc cccagcctgc ggcgcagcgt gaagcgcaac
     1921 agcacggtgg actgcaacgg cgtggtgtcc ctcatcggcg gccccggctc ccacatcggc
     1981 gggcgtctcc tgccagaggc tacaactgag gtggaaatta agaagaaagg ccctggatct
     2041 cttttagttt ccatggacca attagcctcc tacgggcgga aggacagaat caacagtata
     2101 atgagtgttg ttacaaatac actagtagaa gaactggaag agtctcagag aaagtgcccg
     2161 ccatgctggt ataaatttgc caacactttc ctcatctggg agtgccaccc ctactggata
     2221 aaactgaaag agattgtgaa cttgatagtt atggaccctt ttgtggattt agccatcacc
     2281 atctgcatcg tcctgaatac actgtttatg gcaatggagc accatcctat gacaccacaa
     2341 tttgaacatg tcttggctgt aggaaatctg gtaagtatga aattcaggga tacggcatat
     2401 ttgccaaata gtggaaatgt gaagtactga caaaactttt ccctttttcc ctctcatagg
     2461 ttttcactgg aattttcaca gcggaaatgt tcctgaagct catagccatg gatccctact
     2521 attatttcca agaaggttgg aacatttttg acggatttat tgtctccctc agtttaatgg
     2581 aactgagtct agcagacgtg gaggggcttt cagtgctgcg atctttccga ttgctccgag
     2641 tcttcaaatt ggccaaatcc tggcccaccc tgaacatgct aatcaagatt attggaaatt
     2701 cagtgggtgc cctgggcaac ctgacactgg tgctggccat tattgtcttc atctttgccg
     2761 tggtggggat gcaactcttt ggaaaaagct acaaagagtg tgtctgcaag atcaaccagg
     2821 actgtgaact ccctcgctgg catatgcatg actttttcca ttccttcctc attgtctttc
     2881 gagtgttgtg cggggagtgg attgagacca tgtgggactg catggaagtg gcaggccagg
     2941 ccatgtgcct cattgtcttt atgatggtca tggtgattgg caacttggtg gtgctgaacc
     3001 tgtttctggc cttgctcctg agctccttca gtgcagacaa cctggctgcc acagatgacg
     3061 atggggaaat gaacaacctc cagatctcag tgatccgtat caagaagggt gtggcctgga
     3121 ccaaactaaa ggtgcacgcc ttcatgcagg cccactttaa gcagcgtgag gctgatgagg
     3181 tgaagcctct ggatgagttg tatgaaaaga aggccaactg tatcgccaat cacaccggtg
     3241 cagacatcca ccggaatggt gacttccaga agaatggcaa tggcacaacc agcggcattg
     3301 gcagcagcgt ggagaagtac atcattgatg aggaccacat gtccttcatc aacaacccca
     3361 acttgactgt acgggtaccc attgctgtgg gcgagtctga ctttgagaac ctcaacacag
     3421 aggatgttag cagcgagtcg gatcctgaag gcagcaaaga taaactagat gacaccagct
     3481 cctctgaagg aagcaccatt gatatcaaac cagaagtaga agaggtccct gtggaacagc
     3541 ctgaggaata cttggatcca gatgcctgct tcacagaagg ttgtgtccag cggttcaagt
     3601 gctgccaggt caacatcgag gaagggctag gcaagtcttg gtggatcctg cggaaaacct
     3661 gcttcctcat cgtggagcac aactggtttg agaccttcat catcttcatg attctgctga
     3721 gcagtggcgc cctggccttc gaggacatct acattgagca gagaaagacc atccgcacca
     3781 tcctggaata tgctgacaaa gtcttcacct atatcttcat cctggagatg ttgctcaagt
     3841 ggacagccta tggcttcgtc aagttcttca ccaatgcctg gtgttggctg gacttcctca
     3901 ttgtggctgt ctctttagtc agccttatag ctaatgccct gggctactcg gaactaggtg
     3961 ccataaagtc ccttaggacc ctaagagctt tgagaccctt aagagcctta tcacgatttg
     4021 aagggatgag ggtggtggtg aatgccttgg tgggcgccat cccctccatc atgaatgtgc
     4081 tgctggtgtg tctcatcttc tggctgattt tcagcatcat gggagttaac ttgtttgcgg
     4141 gaaagtacca ctactgcttt aatgagactt ctgaaatccg atttgaaatt gaagatgtca
     4201 acaataagac cgaatgtgaa aagcttatgg aggggaacaa tacagagatc agatggaaga
     4261 acgtgaagat caactttgac aatgttgggg caggatacct ggcccttctt caagtagcaa
     4321 ccttcaaagg ctggatggac atcatgtatg cagctgtaga ttcccggaag gtaagtatca
     4381 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
     4441 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     4501 cagcctgatg agcagcctaa gtatgaggac aatatctaca tgtacatcta ttttgtcatc
     4561 ttcatcatct tcggctcctt cttcaccctg aacctgttca ttggtgtcat cattgataac
     4621 ttcaatcaac aaaagaaaaa gttcggaggt caggacatct tcatgaccga agaacagaag
     4681 aagtactaca atgccatgaa aaagctgggc tcaaagaagc cacagaaacc tattccccgc
     4741 cccttgaaca aaatccaagg aatcgtcttt gattttgtca ctcagcaagc ctttgacatt
     4801 gttatcatga tgctcatctg ccttaacatg gtgacaatga tggtggagac agacactcaa
     4861 agcaagcaga tggagaacat cctctactgg attaacctgg tgtttgttat cttcttcacc
     4921 tgtgagtgtg tgctcaaaat gtttgcgttg aggcactact acttcaccat tggctggaac
     4981 atcttcgact tcgtggtagt catcctctcc attgtgggaa tgttcctggc agatataatt
     5041 gagaaatact ttgtttcccc aaccctattc cgagtcatcc gattggcccg tattgggcgc
     5101 atcttgcgtc tgatcaaagg cgccaaaggg attcgtaccc tgctctttgc cttaatgatg
     5161 tccttgcctg ccctgttcaa catcggcctt ctgctcttcc tggtcatgtt catcttctcc
     5221 atttttggga tgtccaattt tgcatatgtg aagcacgagg ctggtatcga tgacatgttc
     5281 aactttgaga catttggcaa cagcatgatc tgcctgtttc aaatcacaac ctcagctggt
     5341 tgggatggcc tgctgctgcc catcctaaac cgcccccctg actgcagcct agataaggaa
     5401 cacccaggga gtggctttaa gggagattgt gggaacccct cagtgggcat cttcttcttt
     5461 gtaagctaca tcatcatctc tttcctaatt gtcgtgaaca tgtacattgc catcatcctg
     5521 gagaacttca gtgtagccac agaggaaagt gcagaccctc tgagtgagga tgactttgag
     5581 accttctatg agatctggga gaagttcgac cccgatgcca cccagttcat tgagtactgt
     5641 aagctggcag actttgcaga tgccttggag catcctctcc gagtgcccaa gcccaatacc
     5701 attgagctca tcgctatgga tctgccaatg gtgagcgggg atcgcatcca ctgcttggac
     5761 atcctttttg ccttcaccaa gcgggtcctg ggagatagcg gggagttgga catcctgcgg
     5821 cagcagatgg aagagcggtt cgtggcatcc aatccttcca aagtgtctta cgagccaatc
     5881 acaaccacac tgcgtcgcaa gcaggaggag gtatctgcag tggtcctgca gcgtgcctac
     5941 cggggacatt tggcaaggcg gggcttcatc tgcaaaaaga caacttctaa taagctggag
     6001 aatggaggca cacaccggga gaaaaaagag agcaccccat ctacagcctc cctcccgtcc
     6061 tatgacagtg taactaaacc tgaaaaggag aaacagcagc gggcagagga aggaagaagg
     6121 gaaagagcca aaagacaaaa agaggtcaga gaatccaagt gttgactcga gcgatcggcc
     6181 cttggatccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     6241 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     6301 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     6361 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     6421 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     6481 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     6541 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     6601 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     6661 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
     6721 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg
     6781 tgagcaaggg cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac atggagggct
     6841 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     6901 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttctcc tgggacatcc
     6961 tgtcccctca gttcatgtac ggctccaggg ccttcaccaa gcaccccgcc gacatccccg
     7021 actactataa gcagtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     7081 acggcggcgc cgtgaccgtg acccaggaca cctccctgga ggacggcacc ctgatctaca
     7141 aggtgaagct ccgcggcacc aacttccctc ctgacggccc cgtaatgcag aagaagacaa
     7201 tgggctggga agcgtccacc gagcggttgt accccgagga cggcgtgctg aagggcgaca
     7261 ttaagatggc cctgcgcctg aaggacggag gccgctacct ggcggacttc aagaccacct
     7321 acaaggccaa gaagcccgtg cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca
     7381 tcacctccca caacgaggac tacaccgtgg tggaacagta cgaacgctcc gagggccgcc
     7441 actccaccgg cggcatggac gagctgtaca agtaacctgc aggaattcaa gggcgaattc
     7501 gtttgctcta gagggcccgc ggttcgaaca aaaactcatc tcagaagagg atctgaatat
     7561 gcataccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     7621 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7681 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7741 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7801 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     7861 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     7921 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7981 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8041 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8101 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8161 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8221 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8281 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     8341 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8401 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8461 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8521 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8581 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8641 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     8701 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     8761 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     8821 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     8881 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     8941 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     9001 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     9061 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     9121 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     9181 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     9241 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     9301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     9421 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9601 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    10081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10921 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    11041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11221 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11581 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
    11641 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
    11701 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
    11761 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
    11821 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
    11881 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
    11941 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
    12001 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
    12061 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
    12121 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
    12181 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
    12241 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
    12301 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
    12361 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
    12421 tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt
    12481 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
    12541 ccgggaccga tccagcctcc ggactctagc gtttaaactt aagcttggta ccgagctcgg
    12601 atccactagt ccagtgtggt ggaattcgcc
//
