LOCUS       p136_pCRISPR-Pka       12216 bp DNA     circular SYN 20-SEP-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12216)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Sep 20, 2023 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    117..416
                     /label=sso
     misc_feature    469..717
                     /label=orit
     CDS             891..1694
                     /codon_start=1
                     /gene="Apramycin+"
                     /label=Apramycin+
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             1950..2450
                     /codon_start=1
                     /label=ori
                     /translation="STERQTP*KRSKDLLEILFFCA*SAACKQKNHRYQRWFVCRIKSY
                     QLFFRR*LASAERRYQILSF*CSRS*ATTSRTL*HRLHTSLC*SCYQWLLPVAISRVLP
                     GWTQDDSYRIRRSGRAERGVRAHSPAWSERPTPN*DTYSVSIEKAPRFPKGERRTGIR*
                     AAGS"
     misc_feature    2324..2450
                     /label=ori
                     /note="ori"
     promoter        3007..3069
                     /label=KasOp*
     -35_signal      3032..3037
                     /label=-35
     primer_bind     3036..3055
                     /label=CW364-kasOP-seq-fwd
     -10_signal      3056..3061
                     /label=-10
     misc_feature    3069
                     /label=TSS
     ncRNA           3088..3169
                     /label=gRNA
     misc_feature    3182..3352
                     /label=terminator
                     /note="terminator"
                     /note="to"
     terminator      3182..3352
     primer_bind     complement(3199..3218)
                     /label=sgRNA_seq_rev
     promoter        3402..3502
     misc_feature    3402..3502
                     /label=p-tipA
                     /note="p-tipA"
     CDS             3535..4222
                     /codon_start=1
                     /product="cytidine deaminase --> C--> U"
                     /label=streAPOBEC1
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             4270..8370
                     /codon_start=1
                     /label=nCas9(D10A)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4270..4293
                     /label=ORF-MCS
                     /note="ORF-MCS"
                     /note="direction: RIGHT"
     CDS             8383..8634
                     /codon_start=1
                     /label=streUGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     misc_feature    8635..8724
                     /label=ORF-MCS
                     /note="ORF-MCS"
                     /note="direction: RIGHT"
     misc_feature    8685..8975
                     /label=fd
                     /note="fd"
                     /note="direction: RIGHT"
     CDS             9206..10015
                     /codon_start=1
                     /label=tsr
                     /translation="MTELDTIANPSDPAVQRIIDVTKPSRSNIKTTLIEDVEPLMHSIA
                     AGVEFIEVYGSDSSPFPSELLDLCGRQNIPVRLIDSSIVNQLFKGERKAKTFGIARVPR
                     PARFGDIASRRGDVVVLDGVKIVGNIGAIVRTSLALGASGIILVDSDITSIADRRLQRA
                     SRGYVFSLPVVLSGREEAIAFIRDSGMQLMTLKADGDISVKELGDNPDRLALLFGSEKG
                     GPSDLFEEASSASVSIPMMSQTESLNVSVSLGIALHERIDRNLAANR"
     rep_origin      10578..12020
                     /label=rep
ORIGIN
        1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
       61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
      121 cgaagcggta cgcgacccgt gccgggccgc agcggatcgc ggcagcagga gctccccgcc
      181 cgccacggct ggctggatgg tcgcctcacg cgcccgcagg ggtgacggcg tgggtgcggc
      241 ggtcagcccc gtgatcgggc gtcagacggc cgctgaggga ccgcgaccac ccctcccttc
      301 ccgcctgccg tctttcaccg cccggccggt ccgcagacgc cgtacggcca ggccacggag
      361 gattacgggc agatggcgcc cacccggcac ccgggctggg gcaccctgga cagcacgaag
      421 gcccgcccca ctcaccgggg cgggccttcg cgttgagcta gagttcccgc cagcctcgca
      481 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
      541 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
      601 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata
      661 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaat gcagctcacg
      721 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
      781 atgccggcct ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta
      841 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
      901 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
      961 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
     1021 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctgcg ctgggtccgg
     1081 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
     1141 cgtcgcccgt tacaccggac cttggagttg tctctgacac attctggcgc ctgccaaatg
     1201 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
     1261 tctctgatcc attgcccctg ccacctcact cgcctgcaag cccggtcgcc cgtgtccatg
     1321 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
     1381 ttgccgagtt gatggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
     1441 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
     1501 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
     1561 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
     1621 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
     1681 agtcgattgg ctgagctcat aagttcctat tccgaagttc ctattcgaaa tgaccgacca
     1741 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     1801 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     1861 gctggagttc ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat
     1921 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1981 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2041 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2101 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2161 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2221 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2281 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2341 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     2401 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2461 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2521 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2581 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2641 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2701 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2761 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2821 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     2881 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     2941 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaattgtac
     3001 aagctttgtt cacattcgaa ccgtctctgc tttgacaaca tgctgtgcgg tgttgtaaag
     3061 tcgtggccag ctagcgtcag atccatggtt ttagagctag aaatagcaag ttaaaataag
     3121 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt acgtaggcgt
     3181 cagctacgat gttccgggga ctgctgatcc ggtcagcagg tggaagaggg actggattcc
     3241 aaagttctca atgctgcttg ctgttcttga atggggggtc gttgacgacg acatggctcg
     3301 attggcgcga caagttgctg cgattctcac caataaaaaa cgcccggcgg caaccgagcg
     3361 ttctgaacaa atccagatgg agttctgagg tcattactgg accggatcgg ggatctgggc
     3421 tgagggagcc gacggcacgc ggcggctcac ggcgtggcac gcggaacgtc cgggcttgca
     3481 cctcacgtca cgtgaggagg cagcgtggac ggcgtcagag aagggagcgg acatatgtcc
     3541 tccgagaccg gccccgtggc cgtggacccg accctgcgtc gccgcatcga gccgcacgag
     3601 ttcgaggtct tcttcgaccc gcgcgagctg cgcaaggaga cctgcctgct gtacgagatc
     3661 aactggggcg gccggcactc catctggcgc cacacctcgc agaacaccaa caagcacgtc
     3721 gaggtcaact tcatcgagaa gttcaccacc gagcggtact tctgcccgaa cacccgctgc
     3781 tccatcacct ggttcctgtc ctggtccccc tgcggcgagt gctcccgggc catcaccgag
     3841 ttcctgtccc gctacccgca cgtcaccctg ttcatctaca tcgcccggct gtaccaccac
     3901 gccgacccgc gcaaccggca gggcctgcgc gacctgatct cctccggcgt caccatccag
     3961 atcatgaccg agcaggagtc cggctactgc tggcggaact tcgtcaacta ctccccgtcc
     4021 aacgaggccc actggccccg ctacccgcac ctgtgggtcc gcctgtacgt cctggagctg
     4081 tactgcatca tcctgggcct gccgccctgc ctgaacatcc tgcgtcgcaa gcagccgcag
     4141 ctgaccttct tcaccatcgc cctccagtcc tgccactacc agcgcctgcc gccccacatc
     4201 ctgtgggcca ccggcctgaa gtccggctcc gagaccccgg gcacctcgga gtccgccacc
     4261 ccggagtccg acaagaagta ctccatcggc ctcgcgatcg gcaccaactc cgtgggctgg
     4321 gcggtcatca ccgacgagta caaggtcccc tccaagaagt tcaaggtcct gggcaacacc
     4381 gaccggcact cgatcaagaa gaacctgatc ggcgccctgc tcttcgacag cggcgagacc
     4441 gccgaggcga cccgcctgaa gcggaccgcg cgtcgccgct acacccggcg caagaaccgc
     4501 atctgctacc tgcaggaaat cttctccaac gagatggcca aggtggacga ctcgttcttc
     4561 caccgcctgg aggagagctt cctggtggag gaggacaaga agcacgagcg ccacccgatc
     4621 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctc
     4681 cgcaagaagc tggtggactc gaccgacaag gcggacctgc ggctcatcta cctggccctc
     4741 gcgcacatga tcaagttccg cggccacttc ctcatcgagg gcgacctgaa cccggacaac
     4801 tccgacgtgg acaagctctt catccagctg gtgcagacct acaaccagct gttcgaggag
     4861 aaccccatca acgccagcgg cgtggacgcc aaggcgatcc tctccgcgcg cctgagcaag
     4921 tcccggcgcc tggagaacct catcgcccag ctgccgggcg agaagaagaa cggcctcttc
     4981 ggcaacctga tcgcgctgtc gctcggcctg acccccaact tcaagagcaa cttcgacctg
     5041 gccgaggacg cgaagctcca gctgtccaag gacacctacg acgacgacct ggacaacctg
     5101 ctcgcccaga tcggcgacca gtacgcggac ctcttcctgg ccgcgaagaa cctctcggac
     5161 gccatcctgc tcagcgacat cctgcgggtc aacaccgaga tcaccaaggc cccgctgtcg
     5221 gcgagcatga tcaagcggta cgacgagcac caccaggacc tgaccctgct caaggccctc
     5281 gtgcgccagc agctgcccga gaagtacaag gaaatcttct tcgaccagtc caagaacggc
     5341 tacgccggct acatcgacgg cggcgcgtcg caggaggagt tctacaagtt catcaagccg
     5401 atcctggaga agatggacgg caccgaggag ctgctcgtca agctgaaccg cgaggacctg
     5461 ctccgcaagc agcggacctt cgacaacggc tccatcccgc accagatcca cctgggcgag
     5521 ctccacgcca tcctccggcg ccaggaggac ttctacccct tcctgaagga caaccgcgag
     5581 aagatcgaga agatcctgac cttccgcatc ccgtactacg tcggccccct ggcccgcggc
     5641 aactcccggt tcgcgtggat gacccggaag tcggaggaga ccatcacccc gtggaacttc
     5701 gaggaggtcg tggacaaggg cgcgtccgcg cagtcgttca tcgagcgcat gaccaacttc
     5761 gacaagaacc tcccgaacga gaaggtcctg cccaagcact ccctgctcta cgagtacttc
     5821 accgtgtaca acgagctgac caaggtcaag tacgtgaccg agggcatgcg gaagccggcc
     5881 ttcctgtcgg gcgagcagaa gaaggcgatc gtggacctgc tcttcaagac caaccgcaag
     5941 gtcaccgtga agcagctgaa ggaggactac ttcaagaaga tcgagtgctt cgactccgtc
     6001 gagatcagcg gcgtggagga ccgcttcaac gcctccctgg gcacctacca cgacctgctc
     6061 aagatcatca aggacaagga cttcctcgac aacgaggaga acgaggacat cctggaggac
     6121 atcgtcctca ccctgaccct cttcgaggac cgcgagatga tcgaggagcg gctcaagacc
     6181 tacgcccacc tgttcgacga caaggtgatg aagcagctga agcgtcgccg ctacaccggc
     6241 tggggccgcc tctcccggaa gctgatcaac ggcatccggg acaagcagag cggcaagacc
     6301 atcctggact tcctcaagtc cgacggcttc gccaaccgca acttcatgca gctcatccac
     6361 gacgacagcc tgaccttcaa ggaggacatc cagaaggccc aggtctcggg ccagggcgac
     6421 agcctccacg agcacatcgc caacctggcg ggctccccgg cgatcaagaa gggcatcctc
     6481 cagaccgtca aggtcgtgga cgagctggtc aaggtgatgg gccgccacaa gcccgagaac
     6541 atcgtgatcg agatggcccg ggagaaccag accacccaga agggccagaa gaactcgcgc
     6601 gagcggatga agcggatcga ggagggcatc aaggagctcg gcagccagat cctgaaggag
     6661 cacccggtcg agaacaccca gctgcagaac gagaagctgt acctctacta cctgcagaac
     6721 ggccgcgaca tgtacgtgga ccaggagctc gacatcaacc ggctgtccga ctacgacgtg
     6781 gaccacatcg tgccgcagtc cttcctgaag gacgactcga tcgacaacaa ggtcctgacc
     6841 cgctcggaca agaaccgggg caagtccgac aacgtgccct cggaggaggt cgtgaagaag
     6901 atgaagaact actggcgcca gctgctcaac gccaagctca tcacccagcg caagttcgac
     6961 aacctgacca aggccgagcg gggcggcctg agcgagctcg acaaggcggg cttcatcaag
     7021 cgccagctgg tcgagacccg gcagatcacc aagcacgtgg cccagatcct ggactcccgg
     7081 atgaacacca agtacgacga gaacgacaag ctgatccgcg aggtcaaggt gatcaccctc
     7141 aagagcaagc tggtctccga cttccgcaag gacttccagt tctacaaggt ccgggagatc
     7201 aacaactacc accacgccca cgacgcgtac ctgaacgccg tcgtgggcac cgcgctgatc
     7261 aagaagtacc cgaagctgga gtccgagttc gtctacggcg actacaaggt ctacgacgtg
     7321 cgcaagatga tcgccaagag cgagcaggag atcggcaagg ccaccgcgaa gtacttcttc
     7381 tactccaaca tcatgaactt cttcaagacc gagatcaccc tggccaacgg cgagatccgc
     7441 aagcggcccc tgatcgagac caacggcgag accggcgaga tcgtctggga caagggccgc
     7501 gacttcgcca ccgtccggaa ggtgctgtcg atgccgcagg tcaacatcgt gaagaagacc
     7561 gaggtgcaga ccggcggctt cagcaaggag tccatcctcc ccaagcgcaa cagcgacaag
     7621 ctgatcgccc ggaagaagga ctgggacccg aagaagtacg gcggcttcga cagccccacc
     7681 gtcgcctact ccgtgctggt cgtggcgaag gtcgagaagg gcaagagcaa gaagctgaag
     7741 tccgtgaagg agctgctcgg catcaccatc atggagcgct cctcgttcga gaagaacccg
     7801 atcgacttcc tggaggccaa gggctacaag gaggtcaaga aggacctcat catcaagctg
     7861 cccaagtaca gcctgttcga gctggagaac ggccgcaagc ggatgctcgc ctccgcgggc
     7921 gagctgcaga agggcaacga gctggccctc ccgtcgaagt acgtcaactt cctgtacctc
     7981 gcgtcccact acgagaagct gaagggctcg cccgaggaca acgagcagaa gcagctcttc
     8041 gtggagcagc acaagcacta cctggacgag atcatcgagc agatcagcga gttcagcaag
     8101 cgcgtcatcc tggccgacgc gaacctcgac aaggtgctgt ccgcctacaa caagcaccgc
     8161 gacaagccga tccgggagca ggcggagaac atcatccacc tgttcaccct caccaacctg
     8221 ggcgcccccg ccgcgttcaa gtacttcgac accaccatcg accgcaagcg gtacacctcc
     8281 accaaggagg tcctcgacgc gaccctgatc caccagagca tcaccggcct gtacgagacc
     8341 cgcatcgacc tgtcccagct cggcggcgac tccggcggct ccaccaacct gtccgacatc
     8401 atcgagaagg agaccggcaa gcagctggtc atccaggagt ccatcctgat gctgccggag
     8461 gaagtcgagg aagtcatcgg caacaagccc gagtccgaca tcctggtcca caccgcctac
     8521 gacgagtcca ccgacgagaa cgtcatgctg ctgacctcgg acgcccccga gtacaagccc
     8581 tgggccctgg tcatccagga ctccaacggc gagaacaaga tcaagatgct gtgatctaga
     8641 ggatccccgg gtaccgagct cgaattcccc agatctaaag ttttgtcgtc tttccagacg
     8701 ttagtaaatg aattttctgt atgaggtttt gctaaacaac tttcaacagt ttcagcggag
     8761 tgagaataga aaggaacaac taaaggaatt gcgaataata attttttcac gttgaaaatc
     8821 tccaaaaaaa aaggctccaa aaggagcctt taattgtatc ggtttatcag cttgctttcg
     8881 aggtgaattt cttaaacagc ttgataccga tagttgcgcc gacaatgaca acaaccatcg
     8941 cccacgcata accgatatat tcggtcgctg aggcttgcag ggagtcaaag gccgcttttg
     9001 cgggatctcg tcgaaggcac tagaggccta tatgcgggga tcgaccgcgc gggtcccgga
     9061 cggggaagag cggggagctt tgccagagag cgacgacttc cccttgcgtt ggtgattgcc
     9121 ggtcagggca gccatccgcc atcgtcgcgt agggtgtcac accccaggaa tcgcgtcact
     9181 gaacacagca gccggtagga cgaccatgac tgagttggac accatcgcaa atccgtccga
     9241 tcccgcggtg cagcggatca tcgatgtcac caagccgtcg cgatccaaca taaagacaac
     9301 gttgatcgag gacgtcgagc ccctcatgca cagcatcgcg gccggggtgg agttcatcga
     9361 ggtctacggc agcgacagca gtccttttcc atctgagttg ctggatctgt gcgggcggca
     9421 gaacataccg gtccgcctca tcgactcctc gatcgtcaac cagttgttca agggggagcg
     9481 gaaggccaag acattcggca tcgcccgcgt ccctcgcccg gccaggttcg gcgatatcgc
     9541 gagccggcgt ggggacgtcg tcgttctcga cggggtgaag atcgtcggga acatcggcgc
     9601 gatagtacgc acgtcgctcg cgctcggagc gtcggggatc atcctggtcg acagtgacat
     9661 caccagcatc gcggaccggc gtctccaaag ggccagccga ggttacgtct tctcccttcc
     9721 cgtcgttctc tccggtcgcg aggaggccat cgccttcatt cgggacagcg gtatgcagct
     9781 gatgacgctc aaggcggatg gcgacatttc cgtgaaggaa ctcggggaca atccggatcg
     9841 gctggccttg ctgttcggca gcgaaaaggg tgggccttcc gacctgttcg aggaggcgtc
     9901 ttccgcctcg gtttccatcc ccatgatgag ccagaccgag tctctcaacg tttccgtttc
     9961 cctcggaatc gcgctgcacg agaggatcga caggaatctc gcggccaacc gataagcgcc
    10021 tctgttcctc ggacgctcgg ttgctcgacc tcgattcgtc agtgatgatc aacaggaccc
    10081 agaaatggca cgagcccggg attggcctcc cgggctcgcc gcccccgaac cggcgcagca
    10141 tgccgagtga ggacgtttgc tctgtgacga gcgtacgaga gaacagcgcg tcgacaccac
    10201 cgcgccccgc cggaccgaag tgccactgcg gcaccccgat cgtccacatc cccgggaagc
    10261 gccccaaaat ctactgctcg gacgcctgcc gaaagcgcgc aaagcgtctc attgcggacc
    10321 agtcacgcag cgtagcaagc acgactacag cgggtaatcc ggacgcgggc tttagtagta
    10381 agggaaaatc tccgtccact gcaggtcaga gcgctgtaac tggaacgggt tctaccccaa
    10441 acgagggtgc cgcgacagcc gggcgggagg acccgcgcga cgagcggttc gcccggcggg
    10501 accggcacca gacggtgtcg ctgaacgagg cgttcaaggg ctgcgggacc cggctcactg
    10561 ccgggacggc cgagctgatg tggaagccgg gcgaggcgac gtggggcaac acgtgccgct
    10621 gcaacaacgt ccacacctgc ccgtggtgca tgagccgcat cctggccgta cggggctcga
    10681 acgtgcagct cgccgccgac ggtctcgcgg acgccgggta cgggctgcac cttggcacga
    10741 acacgttgcg ccacttcgag cgcatggcgt tcggcaccgt ccgcaaggga atgcggcacg
    10801 gcctggtcgc ggtcttgcac gacggctgga agggcgcgta cggctcgtcc ggccgccgct
    10861 ggcgcacgat gcgcgacgac ttcgggatca tcggctacga gcgggccttc gaggacacct
    10921 tcggctgggg ctccggctgg cacctgcact ggcacacgct ctgggtcacc cgcgaggtgc
    10981 tcggcccgga cgcccaggcc gcgttccgcg acgcactcgc cggagcctgg gccgccgggg
    11041 tcgaatccgc gggcggctac accgtcagcg agacgtgcga ccggcccggt tgctcctgtg
    11101 agggcaaggg ccacggcacc gacgtgcgcc cactcaacgg cgcggacgcc gctgacggcg
    11161 atgcgggcaa gcaggcccgc tacctctaca aggacggcga caagaccaag ggcggcgtcg
    11221 ccaagatcgg cctggagctg gccggacaga acttcaaggc cggtcgcggc gacgaccgca
    11281 tgggaccgct cgacctgggc gacgcggcgg ccgccgagct gcagcggctt cgtcggcccg
    11341 gcccgttcgt cgagaagtac cgcgagcgcg agttcggcgt cttccaggtc cgcaagcact
    11401 accggtcgca gaacctgaac cggctgatca aggaactcgg catccagcag gacgtccgca
    11461 ccgaggagga gatcaccgac gacaccgagg gcctggtcgc catcgcggtc atcccggcct
    11521 acatctggta ccgatacatc gcccgcgtcg ccggacgtcg cctcgacctc atcaaggtcg
    11581 ccgagacgta cggcctaccg ggcgtccggc ggctcgtcga gtcctggggc ctggtgtggg
    11641 gcaaggacgt cctggacccg cccgccccgg aagcgccggc cgcgccgggc gaccttgacg
    11701 ctgaccagat gaggttcgag gtcatgtccg aggaggaagc tgctttccgc gaggcgcgcc
    11761 ggaaggccaa cgaggcccgc acggaggagc tggccgcttc gctcgaccga gtgcgtcagc
    11821 cgaagaagga ggcgatccgc ccgacgatca gccttcggaa gcgcctgaag ccgaagccgg
    11881 tcaccgtcga cgtcaagact cccccgcccg gcgccgcgtc gcccgtgtgc cgccgctgca
    11941 agggcaagct cgccccggta ctccagccct ggggccagca ccccggagac tgcctccgcg
    12001 tcgacacagc ggtcgcgtag ggccgctgag ggaccgccat gagaccgcca ccccggacat
    12061 ccgggtggcg gtctcatggc ggtcccggcg cgaaagccga gggtctgcct gccgtgaggt
    12121 ggccggcgga cctgggttac tgctggaatc ccgcgcccag tccgagcgtc cgccggcgct
    12181 tttcacctgg ctccgggtgc tcgtcgatga gcacca
//
