LOCUS       Exported                8975 bp ds-DNA     circular SYN 21-SEP-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    CPY-ymsfGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 21, 2023 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(35..151)
                     /label=NFS1 (fragment)
                     /note="NFS1 (fragment)"
                     /note="pLannotate"
     misc_feature    complement(150..200)
                     /label=bom (fragment)
                     /note="bom (fragment)"
                     /note="pLannotate"
     CDS             complement(302..337)
                     /label=rop (fragment)
                     /note="rop (fragment)"
                     /note="pLannotate"
     misc_feature    complement(406..909)
                     /label=CEN/ARS
                     /note="CEN/ARS"
                     /note="pLannotate"
     promoter        946..1050
                     /label=AmpR promoter
                     /note="AmpR promoter pLannotate"
                     /note="pLannotate"
     CDS             1051..1911
                     /label=AmpR
                     /note="AmpR"
                     /note="pLannotate"
     rep_origin      2082..2670
                     /label=ori
                     /note="ori"
                     /note="pLannotate"
     CDS             2853..2945
                     /label=lacI (fragment)
                     /note="lacI (fragment)"
                     /note="pLannotate"
     protein_bind    2958..2979
                     /label=CAP binding site
                     /note="CAP binding site"
                     /note="pLannotate"
     promoter        2994..3024
                     /label=lac promoter
                     /note="lac promoter pLannotate"
                     /note="pLannotate"
     protein_bind    3032..3048
                     /label=lac operator
                     /note="lac operator"
                     /note="pLannotate"
     promoter        3093..3111
                     /label=T3 promoter
                     /note="T3 promoter pLannotate"
                     /note="pLannotate"
     promoter        3135..3576
                     /label=GAL1 promoter
                     /note="GAL1 promoter pLannotate"
                     /note="pLannotate"
     CDS             3609..5204
                     /codon_start=1
                     /label=PRC1
                     /note="CPY"
                     /note="pLannotate"
                     /translation="MKAFTSLLCGLGLSTTLAKAISLQRPLGLDKDVLLQAAEKFGLDL
                     DLDHLLKELDSNVLDAWAQIEHLYPNQVMSLETSTKPKFPEAIKTKKDWDFVVKNDAIE
                     NYQLRVNKIKDPKILGIDPNVTQYTGYLDVEDEDKHFFFWTFESRNDPAKDPVILWLNG
                     GPGCSSLTGLFFELGPSSIGPDLKPIGNPYSWNSNATVIFLDQPVNVGFSYSGSSGVSN
                     TVAAGKDVYNFLELFFDQFPEYVNKGQDFHIAGESYAGHYIPVFASEILSHKDRNFNLT
                     SVLIGNGLTDPLTQYNYYEPMACGEGGEPSVLPSEECSAMEDSLERCLGLIESCYDSQS
                     VWSCVPATIYCNNAQLAPYQRTGRNVYDIRKDCEGGNLCYPTLQDIDDYLNQDYVKEAV
                     GAEVDHYESCNFDINRNFLFAGDWMKPYHTAVTDLLNQDLPILVYAGDKDFICNWLGNK
                     AWTDVLPWKYDEEFASQKVRNWTASITDEVAGEVKSYKHFTYLRVFNGGHMVPFDVPEN
                     ALSMVNEWIHGGFSL"
     CDS             5208..5924
                     /codon_start=1
                     /label=moxGFP
                     /note="moxGFP"
                     /note="pLannotate"
                     /translation="LVSKGEEFFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      5940..6187
                     /label=CYC1 terminator
                     /note="CYC1 terminator pLannotate"
                     /note="pLannotate"
     promoter        complement(6206..6224)
                     /label=T7 promoter
                     /note="T7 promoter pLannotate"
                     /note="pLannotate"
     CDS             6232..6387
                     /label=lacZ-alpha (fragment)
                     /note="lacZ-alpha (fragment)"
                     /note="pLannotate"
     rep_origin      6391..6819
                     /label=f1 ori
                     /note="f1 ori"
                     /note="pLannotate"
     misc_feature    complement(6847..6899)
                     /label=bom (fragment)
                     /note="bom (fragment)"
                     /note="pLannotate"
     CDS             6930..7001
                     /label=YO192_YEAST (fragment)
                     /note="YO192_YEAST (fragment)"
                     /note="pLannotate"
     promoter        7147..7554
                     /label=LEU2 promoter
                     /note="LEU2 promoter pLannotate"
                     /note="pLannotate"
     CDS             7555..8649
                     /label=LEU2
                     /note="LEU2"
                     /note="pLannotate"
ORIGIN
        1 aaaaaaaaaa aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg atggagttta
       61 agtcaatacc ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc
      121 tgtcagaaac ggccttaacg acgtagtcga tatggtgcac tctcagtaca atctgctctg
      181 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      241 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
      301 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
      361 tatttttata ggttaatgtc atgataataa tggtttctta ggacggatcg cttgcctgta
      421 acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta
      481 tttattttta tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac
      541 ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac
      601 atatatattt attagacaag aaaagcagat taaatagata tacattcgat taacgataag
      661 taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat
      721 tcggcattaa tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc
      781 ccctagagtc ttttacatct tcggaaaaca aaaactattt tttctttaat ttcttttttt
      841 actttctatt tttaatttat atatttatat taaaaaattt aaattataat tatttttata
      901 gcacgtgatg aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      961 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1021 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1081 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1141 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1201 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1261 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1321 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1381 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1441 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1501 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1561 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1621 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1681 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1741 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1801 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1861 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1921 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1981 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2041 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2101 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2161 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2221 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2281 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2341 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2401 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2461 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2521 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2581 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2641 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2701 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2761 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2821 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2881 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2941 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     3001 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     3061 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     3121 ctggagctct agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact
     3181 ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc
     3241 cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa
     3301 aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag
     3361 gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg
     3421 atttttgatc tattaacaga tatataaatg caaaaactgc ataaccactt taactaatac
     3481 tttcaacatt ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa
     3541 attgttaata tacctctata ctttaacgtc aaggagaaaa aaccccggat tctagaacta
     3601 gtctaaaaat gaaagcattc accagtttac tatgtggact aggcctgtcc actacactcg
     3661 ctaaggccat ctcattgcaa agaccgttgg gtctagataa ggacgttttg ctgcaagctg
     3721 cggaaaaatt tggtttggac ctcgacctgg atcatctctt gaaggagttg gactccaatg
     3781 tattggacgc ttgggcccaa atagagcatt tgtacccaaa ccaggttatg agccttgaaa
     3841 cttccactaa gccaaaattc cctgaagcaa tcaaaacgaa gaaagactgg gactttgtgg
     3901 tcaagaatga cgcaattgaa aactatcagc ttcgtgtcaa caagattaag gaccctaaaa
     3961 tcctgggcat tgacccaaat gtcacacagt acacgggtta cttggatgtg gaagacgagg
     4021 acaagcattt cttcttttgg acttttgaaa gtagaaacga tcctgcaaag gatccggtca
     4081 tcctttggtt gaacgggggt ccaggttgtt cttcactaac cgggctgttc tttgaattag
     4141 gaccctcatc cattggacct gatttgaaac ccatcgggaa cccttactct tggaacagca
     4201 atgccaccgt gatcttcctt gaccagcctg tcaacgttgg gttctcgtat tccgggtcct
     4261 caggtgtttc caacactgtc gccgctggta aggatgtcta taacttcttg gagttgttct
     4321 tcgatcagtt ccctgaatac gtcaacaagg gccaagattt ccacatcgct ggggaatcct
     4381 acgccggcca ttacatccct gtttttgcct ctgaaatttt gtctcacaag gacagaaact
     4441 tcaacttaac ctccgtcttg atcggaaatg gcctcactga cccattgact cagtataact
     4501 attacgaacc aatggcctgt ggtgaaggtg gcgaaccatc tgttttgccc tcggaggaat
     4561 gctctgctat ggaagactct ttggaacgtt gtttgggctt gatcgagtcg tgctatgact
     4621 cgcaatccgt ctggtcctgt gttccagcta ccatttattg taataacgcc caattggctc
     4681 cttaccaacg taccggcaga aacgtttacg atatcaggaa ggattgtgaa ggtggcaatt
     4741 tgtgctaccc aacgttacaa gatatcgacg actacttaaa ccaggactac gtcaaagaag
     4801 ctgtcggtgc ggaggttgac cactacgaat cctgtaactt cgatatcaac agaaatttcc
     4861 tgtttgcggg tgattggatg aagccttacc acaccgccgt aacagatctt ttgaatcaag
     4921 acctacccat tctggtatat gcaggcgata aagatttcat ctgtaactgg ttgggtaata
     4981 aggcgtggac ggatgtcttg ccatggaagt acgacgaaga atttgcaagc caaaaagtac
     5041 gtaactggac tgcttctatc accgacgagg tcgctggtga agtcaaatcc tacaagcact
     5101 tcacctattt gagagtcttc aatggtggcc acatggttcc atttgacgtc cctgaaaacg
     5161 ccttaagtat ggttaacgaa tggatccacg gtggtttctc cttaaagctt gtatccaaag
     5221 gtgaagaatt ctttactggt gttgttccta ttttagttga attagacggt gacgttaatg
     5281 gtcataagtt tagtgttaga ggtgaaggtg aaggtgacgc aactaacggt aaattgacat
     5341 tgaagtttat ttgtactact ggtaaattgc cagttccatg gccaaccttg gttaccactt
     5401 taacttatgg tgttcaatgc ttctcaagat accctgatca tatgaaaaga cacgactttt
     5461 tcaagagtgc catgccagaa ggttacgtac aagaaagaac tatatctttt aaagatgacg
     5521 gtacatacaa gaccagagct gaagtaaaat tcgaaggtga cacattggtc aacagaatcg
     5581 aattgaaggg tatcgatttc aaggaagacg gtaacatctt gggtcataaa ttagaataca
     5641 atttcaactc acacaatgtt tacatcactg ctgataagca aaagaatggt atcaaagcaa
     5701 acttcaagat cagacataac gttgaagatg gttccgtaca attagctgac cactatcaac
     5761 aaaatacacc tattggtgac ggtcctgttt tgttaccaga caaccattac ttgtctaccc
     5821 aatcaaaatt gtccaaagat ccaaacgaaa agagagacca tatggtcttg ttggaatttg
     5881 tcactgccgc tggtatcact cacggtatgg atgaattgta taagtaggtc gacctcgagt
     5941 catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga
     6001 aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt
     6061 agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta
     6121 cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt
     6181 aatttgcggc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     6241 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6301 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6361 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     6421 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6481 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6541 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6601 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6661 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6721 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6781 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6841 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatc
     6901 gaccggtcga ggagaacttc tagtatatct acatacctaa tattattgcc ttattaaaaa
     6961 tggaatccca acaattacat caaaatccac attctcttca aaatcaattg tcctgtactt
     7021 ccttgttcat gtgtgttcaa aaacgttata tttataggat aattatactc tatttctcaa
     7081 caagtaattg gttgtttggc cgagcggtct aaggcgcctg attcaagaaa tatcttgacc
     7141 gcagttaact gtgggaatac tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa
     7201 ccattatttt tttcctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa
     7261 ttcgatgact ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca
     7321 actgaaaaat tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt tcatatagaa
     7381 tagagaagcg ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt
     7441 aaggacctat tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt
     7501 accttttaca tttcagcaat atatatatat atatttcaag gatataccat tctaatgtct
     7561 gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa
     7621 gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa
     7681 aatcatttaa ttggtggtgc tgctatcgat gctacaggtg ttccacttcc agatgaggcg
     7741 ctggaagcct ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg
     7801 ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg
     7861 tacgccaact taagaccatg taactttgca tccgactctc ttttagactt atctccaatc
     7921 aagccacaat ttgctaaagg tactgacttc gttgttgtca gagaattagt gggaggtatt
     7981 tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac
     8041 accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag
     8101 ccaccattgc ctatttggtc cttggataaa gctaatgttt tggcctcttc aagattatgg
     8161 agaaaaactg tggaggaaac catcaagaac gaattcccta cattgaaggt tcaacatcaa
     8221 ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata
     8281 atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggttcc
     8341 ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt
     8401 ttgtacgaac catgccacgg ttctgctcca gatttgccaa agaataaggt caaccctatc
     8461 gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt
     8521 aaggccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta
     8581 ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc
     8641 cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat aaatgaaatt
     8701 cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat
     8761 atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg
     8821 taagcaaatt gatactaatg gctcaacgtg ataaggaaaa agaattgcac tttaacatta
     8881 atattgacaa ggaggagggc accacacaaa aagttaggtg taacagaaaa tcatgaaact
     8941 atgattccta atttatatat tggaggattt tctct
//
