LOCUS       pBTK1052                2657 bp ds-DNA     circular     20-SEP-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    76..97
                     /label="mb_KanR_Ori_Seq"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(100..247)
                     /label="KanR Promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      248..248
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(248..248)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
     misc_feature    259..280
                     /label="JP.YTK-3-F2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      complement(261..369)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(261..369)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     cds             complement(370..1029)
                     /label="CamR"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
     misc_feature    complement(382..402)
                     /label="mb_CamR-Rev-Seq"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(1030..1134)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     rep_origin      complement(1135..1898)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
     misc_feature    1738..1758
                     /label="mb_YTK_ColE_Seq"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(1876..1902)
                     /label="JP.YTK-3-R"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      complement(1914..1940)
                     /label="KanR Terminator"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     cds             complement(1941..99)
                     /label="KanR"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
ORIGIN
        1 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
       61 cggcctggag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
      121 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
      181 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa
      241 ggatcagTAT GTGAGACCag accaataaaa aacgcccggc ggcaaccgag cgttctgaac
      301 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
      361 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
      421 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
      481 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
      541 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa
      601 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
      661 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
      721 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
      781 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
      841 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
      901 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
      961 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     1021 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     1081 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
     1141 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1201 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1261 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1321 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1381 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1441 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1501 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1561 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1621 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1681 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1741 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1801 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1861 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggTCTCAA ACGgtgttac
     1921 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     1981 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     2041 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     2101 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     2161 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
     2221 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     2281 ccgttattca ttcgtgattg cgcctgagcg aggcgaaata cgcgatcgct gttaaaagga
     2341 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     2401 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
     2461 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     2521 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     2581 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
     2641 gtcgcacctg attgccc
//