LOCUS       sABE_v3.22-N_isN        4008 bp ds-DNA     circular     27-SEP-2023
DEFINITION  .
COMMENT     Golden Gate/Type IIS Cloning ligation productGolden Gate/Type IIS
            Cloning ligation product
FEATURES             Location/Qualifiers
     rep_origin      609..1282
                     /label="Rep_Origin_1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="NCBI Feature Key: rep_origin"
     terminator      complement(1553..1580)
                     /label="rrnb T2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="NCBI Feature Key: terminator"
     primer          1674..1700
                     /label="RBS3236*PE-CMV-F"
                     /note="sequence: TAGTAGGTCTCACTCAACGCGTTGACATTGATTATTGAC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     enhancer        1685..2064
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: human cytomegalovirus immediate early enhancer"
     promoter        2065..2268
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: human cytomegalovirus (CMV) immediate early promoter"
     promoter        2310..2328
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="note: promoter for bacteriophage T7 RNA polymerase NCBI Feature Key: promoter"
     CDS             2342..2398
                     /label="Translation 2342-2398"
                     /translation="MKRTADGSEFESPKKKRKV"
     mat_peptide     2345..2398
                     /label="BPSV40"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created by: madacheng"
     mat_peptide     2399..2623
                     /label="ABE8e N-75"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="created by: madacheng"
     CDS             2399..2623
                     /label="Translation 2399-2623"
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLI"
     mat_peptide     2624..2671
                     /label="linker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="created by: madacheng"
     CDS             2624..2671
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="GGSGGSSGGSSGGGGS"
     mat_peptide     2672..2947
                     /label="FRB"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="created by: madacheng"
     CDS             2672..2947
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="SILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRI"
     primer          complement(2928..2954)
                     /label="RBS1342*d620-chainB-1-R"
                     /note="sequence: TAGTAGGTCTCACCGAGCTGATCCTCCTGAACACGTGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     mat_peptide     2948..2968
                     /label="linker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="created by: madacheng"
     primer          2951..2984
                     /label="RBS1341*d620-chainB-2-F"
                     /note="sequence: TAGTAGGTCTCATCGGGCGGTGGCGGCTCGAGCATCCTGTGGCATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     mat_peptide     2969..3247
                     /label="FRB"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="created by: madacheng"
     CDS             2969..3247
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="SILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRIS"
     primer          complement(3225..3254)
                     /label="RBS1340*d620-chainB-R-cc"
                     /note="sequence: TAGTAGGTCTCATAGTTTAGCTGATCCTCCTGAACACGTGGT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     promoter        complement(3298..3316)
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: promoter for bacteriophage SP6 RNA polymerase NCBI Feature Key: promoter"
     polyA_signal    3342..3566
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: bovine growth hormone polyadenylation signal NCBI Feature Key: polyA_signal"
     mat_peptide     3687..488
                     /label="kanR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="created by: madacheng"
ORIGIN
        1 TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC
       61 GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTTG
      121 CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC
      181 GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC
      241 CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA
      301 AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG
      361 CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA
      421 AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT
      481 TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC
      541 GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG
      601 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA
      661 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG
      721 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG
      781 TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT
      841 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA
      901 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG
      961 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC
     1021 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA
     1081 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC
     1141 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT
     1201 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
     1261 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
     1321 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
     1381 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
     1441 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC
     1501 GCAACGCAAT TAATACGCGT ACCGCTAGCC AGGAAGAGTT TGTAGAAACG CAAAAAGGCC
     1561 ATCCGTCAGG ATGGCCTTCT GCTTAGTTTG ATGCCTGGCA GTTTATGGCG GGCGTCCTGC
     1621 CCGCCACCCT CCGGGCCGTT GCTTCACAAC GTTCAAATCC GCTCCCGGCC CATCTCAACG
     1681 CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT
     1741 AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG
     1801 CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA
     1861 GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA
     1921 CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC
     1981 GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC
     2041 GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA
     2101 TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG
     2161 TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG
     2221 CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC
     2281 CGTCAGATCC GCTAGAGATC CGCGGCCGCT AATACGACTC ACTATAGGGA GAGCCGCCAC
     2341 CATGAAACGG ACAGCCGACG GAAGCGAGTT CGAGTCACCA AAGAAGAAGC GGAAAGTCTC
     2401 TGAGGTGGAG TTTTCCCACG AGTACTGGAT GAGACATGCC CTGACCCTGG CCAAGAGGGC
     2461 ACGGGATGAG AGGGAGGTGC CTGTGGGAGC CGTGCTGGTG CTGAACAATA GAGTGATCGG
     2521 CGAGGGCTGG AACAGAGCCA TCGGCCTGCA CGACCCAACA GCCCATGCCG AAATTATGGC
     2581 CCTGAGACAG GGCGGCCTGG TCATGCAGAA CTACAGACTG ATTGGAGGCT CTGGAGGAAG
     2641 CAGCGGCGGC AGCTCGGGCG GTGGCGGCTC GAGCATCCTG TGGCATGAGA TGTGGCACGA
     2701 GGGCCTGGAG GAGGCCAGCA GGCTGTATTT CGGCGAGAGG AATGTGAAGG GCATGTTCGA
     2761 GGTGCTGGAG CCCCTGCACG CCATGATGGA AAGGGGCCCA CAGACCCTGA AGGAAACTAG
     2821 CTTCAACCAG GCCTACGGCA GGGACCTGAT GGAGGCCCAG GAGTGGTGCA GGAAGTACAT
     2881 GAAGAGCGGC AACGTGAAGG ATCTGACCCA GGCTTGGGAC TTGTACTACC ACGTGTTCAG
     2941 GAGGATCAGC TCGGGCGGTG GCGGCTCGAG CATCCTGTGG CATGAGATGT GGCACGAGGG
     3001 CCTGGAGGAG GCCAGCAGGC TGTATTTCGG CGAGAGGAAT GTGAAGGGCA TGTTCGAGGT
     3061 GCTGGAGCCC CTGCACGCCA TGATGGAAAG GGGCCCACAG ACCCTGAAGG AAACTAGCTT
     3121 CAACCAGGCC TACGGCAGGG ACCTGATGGA GGCCCAGGAG TGGTGCAGGA AGTACATGAA
     3181 GAGCGGCAAC GTGAAGGATC TGACCCAGGC TTGGGACTTG TACTACCACG TGTTCAGGAG
     3241 GATCAGCTAA ACTACGAACC CCGGGTAAAT GCATGAATTC GATCTAGAGG GCCCTATTCT
     3301 ATAGTGTCAC CTAAATGCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA
     3361 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC
     3421 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT
     3481 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA
     3541 TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGGGCCCGTG TCTCAAAATC
     3601 TCTGATGTTA CATTGCACAA GATAAAATAA TATCATCATG AACAATAAAA CTGTCTGCTT
     3661 ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG TCGAGGCCGC
     3721 GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG
     3781 GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC
     3841 TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT
     3901 GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG
     3961 CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT CCAGGTAT
//