LOCUS       3Lenti-Lenti#3_sABE_v3 10240 bp ds-DNA     circular     27-SEP-2023
DEFINITION  .
COMMENT     Ligation of 2 sequencesLigation of 2 sequences
FEATURES             Location/Qualifiers
     misc_feature                                    1..189
                                                     /label="5' LTR"
                                                     /ApEinfo_revcolor="#84b0dc"
                                                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature                                    235..360
                                                     /label="HIV-1 Psi"
                                                     /ApEinfo_revcolor="#b1ff67"
                                                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature                                    240..377
                                                     /label="psi"
                                                     /ApEinfo_revcolor="#d6b295"
                                                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature                                    297..300
                                                     /label="SD"
                                                     /ApEinfo_revcolor="#ff9ccd"
                                                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature                                    853..1086
                                                     /label="RRE"
                                                     /ApEinfo_revcolor="#d6b295"
                                                     /ApEinfo_fwdcolor="#d6b295"
     CDS                                             1271..1315
                                                     /label="gp41 peptide"
                                                     /ApEinfo_revcolor="#ffef86"
                                                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature                                    1613..1730
                                                     /label="cPPT/CTS"
                                                     /ApEinfo_revcolor="#b4abac"
                                                     /ApEinfo_fwdcolor="#b4abac"
     primer                                          1774..1795
                                                     /label="RBS3531*U6-F-CTCC-lenti"
                                                     /note="sequence: TAGTAGGTCTCACTccCCCACCTCCCAACCCCG"
                                                     /ApEinfo_revcolor="#f7977a"
                                                     /ApEinfo_fwdcolor="#f7977a"
     primer_bind                                     1808..1828
                                                     /label="hU6-F"
                                                     /ApEinfo_revcolor="#c6c9d1"
                                                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter                                        1808..2048
                                                     /label="U6 promoter"
                                                     /ApEinfo_revcolor="#f8d3a9"
                                                     /ApEinfo_fwdcolor="#f8d3a9"
                                                     /note="note: RNA polymerase III promoter for human U6 snRNA NCBI Feature Key: promoter .hidden_Parent: 1v50vxE2anE2fsQsq1YWDh5JLCU.1618413825603.71"
     invisible_ActivelyLinkedChildDelete_primer_bind 1979..1998
                                                     /label="LKO.1 5'"
                                                     /ApEinfo_revcolor="#84b0dc"
                                                     /ApEinfo_fwdcolor="#84b0dc"
                                                     /note="note: Human U6 promoter, forward primer NCBI Feature Key: primer_bind"
     misc_RNA                                        2077..2152
                                                     /label="gRNA scaffold"
                                                     /ApEinfo_revcolor="#f58a5e"
                                                     /ApEinfo_fwdcolor="#f58a5e"
                                                     /note="note: guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system NCBI Feature Key: misc_RNA"
     primer                                          complement(2160..2188)
                                                     /label="RBS3502*U6-R"
                                                     /note="sequence: TAGTAGGTCTCACGAATCTCAAGATCTAGTTACGCCAAGCT"
                                                     /ApEinfo_revcolor="#f7977a"
                                                     /ApEinfo_fwdcolor="#f7977a"
     primer                                          2184..2205
                                                     /label="RBS3505*CMV-TTCG-F"
                                                     /note="sequence: TAGTAGGTCTCATTCGCGCTCCCGGCCCATCTC"
                                                     /ApEinfo_revcolor="#f7977a"
                                                     /ApEinfo_fwdcolor="#f7977a"
     enhancer                                        2214..2593
                                                     /label="CMV enhancer"
                                                     /ApEinfo_revcolor="#84b0dc"
                                                     /ApEinfo_fwdcolor="#84b0dc"
                                                     /note="note: human cytomegalovirus immediate early enhancer"
     promoter                                        2594..2797
                                                     /label="CMV promoter"
                                                     /ApEinfo_revcolor="#75c6a9"
                                                     /ApEinfo_fwdcolor="#75c6a9"
                                                     /note="note: human cytomegalovirus (CMV) immediate early promoter"
     promoter                                        2839..2857
                                                     /label="T7 promoter"
                                                     /ApEinfo_revcolor="#75c6a9"
                                                     /ApEinfo_fwdcolor="#75c6a9"
                                                     /note="note: promoter for bacteriophage T7 RNA polymerase NCBI Feature Key: promoter"
     invisible_ActivelyLinkedChildDelete_primer_bind 2839..2858
                                                     /label="T7"
                                                     /ApEinfo_revcolor="#d6b295"
                                                     /ApEinfo_fwdcolor="#d6b295"
                                                     /note="note: T7 promoter, forward primer NCBI Feature Key: primer_bind"
     misc_feature                                    2865..2874
                                                     /label="Kozak Sequence"
                                                     /ApEinfo_revcolor="#d6b295"
                                                     /ApEinfo_fwdcolor="#d6b295"
     mat_peptide                                     2874..2927
                                                     /label="BPSV40 NLS"
                                                     /ApEinfo_revcolor="#faac61"
                                                     /ApEinfo_fwdcolor="#faac61"
     misc_feature                                    2928..3152
                                                     /label="TadA-8e (2-76)"
                                                     /ApEinfo_revcolor="#c6c9d1"
                                                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature                                    3153..3200
                                                     /label="Linker"
                                                     /ApEinfo_revcolor="#b7e6d7"
                                                     /ApEinfo_fwdcolor="#b7e6d7"
                                                     /note="created by: madacheng"
     misc_feature                                    3201..3479
                                                     /label="FRB"
                                                     /ApEinfo_revcolor="#b1ff67"
                                                     /ApEinfo_fwdcolor="#b1ff67"
                                                     /note="created by: madacheng"
     misc_feature                                    3480..3497
                                                     /label="linker"
                                                     /ApEinfo_revcolor="#d6b295"
                                                     /ApEinfo_fwdcolor="#d6b295"
                                                     /note="created by: madacheng"
     misc_feature                                    3498..3776
                                                     /label="FRB"
                                                     /ApEinfo_revcolor="#75c6a9"
                                                     /ApEinfo_fwdcolor="#75c6a9"
                                                     /note="created by: madacheng"
     misc_feature                                    3985..4550
                                                     /label="WPRE"
                                                     /ApEinfo_revcolor="#ffef86"
                                                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind                                     complement(4015..4035)
                                                     /label="WPRE-R"
                                                     /ApEinfo_revcolor="#c7b0e3"
                                                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS                                             complement(4433..4444)
                                                     /label="Factor Xa site"
                                                     /ApEinfo_revcolor="#faac61"
                                                     /ApEinfo_fwdcolor="#faac61"
     misc_feature                                    4755..5256
                                                     /label="3' LTR"
                                                     /ApEinfo_revcolor="#f8d3a9"
                                                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature                                    5281..5494
                                                     /label="BGH PolyA"
                                                     /ApEinfo_revcolor="#b7e6d7"
                                                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal                                    5287..5511
                                                     /label="bGH poly(A) signal"
                                                     /ApEinfo_revcolor="#faac61"
                                                     /ApEinfo_fwdcolor="#faac61"
     rep_origin                                      5557..5969
                                                     /label="f1 ori"
                                                     /ApEinfo_revcolor="#f58a5e"
                                                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin                                      5574..5880
                                                     /label="F1 ori"
                                                     /ApEinfo_revcolor="#9eafd2"
                                                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind                                     complement(5644..5663)
                                                     /label="F1ori-R"
                                                     /ApEinfo_revcolor="#d59687"
                                                     /ApEinfo_fwdcolor="#d59687"
     primer_bind                                     5854..5875
                                                     /label="F1ori-F"
                                                     /ApEinfo_revcolor="#c7b0e3"
                                                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin                                      6035..6359
                                                     /label="SV40 ori"
                                                     /ApEinfo_revcolor="#d59687"
                                                     /ApEinfo_fwdcolor="#d59687"
     primer_bind                                     6241..6260
                                                     /label="SV40pro-F"
                                                     /ApEinfo_revcolor="#85dae9"
                                                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature                                    6375..6440
                                                     /label="EM7"
                                                     /ApEinfo_revcolor="#ff9ccd"
                                                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS                                             6442..6816
                                                     /label="Zeocin(r)"
                                                     /ApEinfo_revcolor="#b1ff67"
                                                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal                                    6946..7067
                                                     /label="SV40 poly(A) signal"
                                                     /ApEinfo_revcolor="#f58a5e"
                                                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind                                     complement(6983..7002)
                                                     /label="SV40pA-R"
                                                     /ApEinfo_revcolor="#b4abac"
                                                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind                                     7037..7056
                                                     /label="EBV-rev"
                                                     /ApEinfo_revcolor="#c7b0e3"
                                                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind                                     complement(7112..7132)
                                                     /label="M13-rev"
                                                     /ApEinfo_revcolor="#85dae9"
                                                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind                                     complement(7116..7132)
                                                     /label="M13 Reverse"
                                                     /ApEinfo_revcolor="#c7b0e3"
                                                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind                                     complement(7116..7132)
                                                     /label="M13 rev"
                                                     /ApEinfo_revcolor="#f8d3a9"
                                                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind                                     complement(7129..7151)
                                                     /label="M13/pUC Reverse"
                                                     /ApEinfo_revcolor="#f58a5e"
                                                     /ApEinfo_fwdcolor="#f58a5e"
     misc_binding                                    complement(7138..7160)
                                                     /label="LacO"
                                                     /ApEinfo_revcolor="#84b0dc"
                                                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind                                    complement(7140..7156)
                                                     /label="lac operator"
                                                     /ApEinfo_revcolor="#b1ff67"
                                                     /ApEinfo_fwdcolor="#b1ff67"
     promoter                                        complement(7164..7194)
                                                     /label="lac promoter"
                                                     /ApEinfo_revcolor="#c7b0e3"
                                                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind                                    complement(7209..7230)
                                                     /label="CAP binding site"
                                                     /ApEinfo_revcolor="#f8d3a9"
                                                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind                                     complement(7347..7364)
                                                     /label="L4440"
                                                     /ApEinfo_revcolor="#f58a5e"
                                                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin                                      complement(7459..8132)
                                                     /label="pUC ori"
                                                     /ApEinfo_revcolor="#b1ff67"
                                                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin                                      complement(7500..8182)
                                                     /label="ColE1 origin"
                                                     /ApEinfo_revcolor="#9eafd2"
                                                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin                                      complement(7518..8106)
                                                     /label="ori"
                                                     /ApEinfo_revcolor="#b7e6d7"
                                                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind                                     complement(7598..7617)
                                                     /label="pBR322ori-F"
                                                     /ApEinfo_revcolor="#b4abac"
                                                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature                                    7705..7930
                                                     /label="ColE1"
                                                     /ApEinfo_revcolor="#d6b295"
                                                     /ApEinfo_fwdcolor="#d6b295"
     promoter                                        8183..8316
                                                     /label="Spn\promoter"
                                                     /ApEinfo_revcolor="#faac61"
                                                     /ApEinfo_fwdcolor="#faac61"
                                                     /note="vntifkey: 30 NCBI Feature Key: promoter"
     misc_feature                                    8317..9327
                                                     /label="SpnR"
                                                     /ApEinfo_revcolor="#ffef86"
                                                     /ApEinfo_fwdcolor="#ffef86"
                                                     /note="gene: SpnR invitrogen: 2750000 vntifkey: 21 NCBI Feature Key: misc_feature"
     misc_feature                                    complement(9329..9378)
                                                     /label="bla"
                                                     /ApEinfo_revcolor="#c6c9d1"
                                                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature                                    9625..9644
                                                     /label="PCMV"
                                                     /ApEinfo_revcolor="#b4abac"
                                                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature                                    9645..10032
                                                     /label="PCMV"
                                                     /ApEinfo_revcolor="#ff9ccd"
                                                     /ApEinfo_fwdcolor="#ff9ccd"
     enhancer                                        9651..10030
                                                     /label="CMV enhancer"
                                                     /ApEinfo_revcolor="#b1ff67"
                                                     /ApEinfo_fwdcolor="#b1ff67"
     promoter                                        9727..10030
                                                     /label="CMV enhancer"
                                                     /ApEinfo_revcolor="#c7b0e3"
                                                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 CTGTACTGGG TGTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG
       61 GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG
      121 TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT
      181 CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG GGAAACCAGA GGAGCTCTCT
      241 CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG GCGAGGGGCG GCGACTGGTG
      301 AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA GAGATGGGTG CGAGAGCGTC
      361 AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA TTCGGTTAAG GCCAGGGGGA
      421 AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA GGGAGCTAGA ACGATTCGCA
      481 GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC AAATACTGGG ACAGCTACAA
      541 CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT ATAATACAGT AGCAACCCTC
      601 TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG AAGCTTTAGA CAAGATAGAG
      661 GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG CCGCTGATCT TCAGACCTGG
      721 AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT
      781 TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG
      841 AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG
      901 CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA
      961 GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG
     1021 GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA
     1081 GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA
     1141 TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG
     1201 GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA
     1261 CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA
     1321 TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG
     1381 CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG
     1441 ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA
     1501 AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG
     1561 ATCGGCACTG CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA
     1621 GAAAAGGGGG GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG
     1681 ACATACAAAC TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT
     1741 ACAGGGACAG CAGAGATCCA GTTTGGTTAA TTAACTCCCC CACCTCCCAA CCCCGAGGGG
     1801 ACCCAGAGAG GGCCTATTTC CCATGATTCC TTCATATTTG CATATACGAT ACAAGGCTGT
     1861 TAGAGAGATA ATTAGAATTA ATTTGACTGT AAACACAAAG ATATTAGTAC AAAATACGTG
     1921 ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA AAATTATGTT TTAAAATGGA
     1981 CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
     2041 GAAAGGACGA AACACCGAAC ACAAAGCATA GACTGCGTTT TAGAGCTAGA AATAGCAAGT
     2101 TAAAATAAGG CTAGTCCGTT ATCAACTTGA AAAAGTGGCA CCGAGTCGGT GCTTTTTTAA
     2161 GCTTGGCGTA ACTAGATCTT GAGATTCGCG CTCCCGGCCC ATCTCAACGC GTTGACATTG
     2221 ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT
     2281 TGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC
     2341 CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA
     2401 TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA
     2461 TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA
     2521 TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT
     2581 CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA
     2641 CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA
     2701 AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCGG
     2761 TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTGGT TTAGTGAACC GTCAGATCCG
     2821 CTAGAGATCC GCGGCCGCTA ATACGACTCA CTATAGGGAG AGCCGCCACC ATGAAACGGA
     2881 CAGCCGACGG AAGCGAGTTC GAGTCACCAA AGAAGAAGCG GAAAGTCTCT GAGGTGGAGT
     2941 TTTCCCACGA GTACTGGATG AGACATGCCC TGACCCTGGC CAAGAGGGCA CGGGATGAGA
     3001 GGGAGGTGCC TGTGGGAGCC GTGCTGGTGC TGAACAATAG AGTGATCGGC GAGGGCTGGA
     3061 ACAGAGCCAT CGGCCTGCAC GACCCAACAG CCCATGCCGA AATTATGGCC CTGAGACAGG
     3121 GCGGCCTGGT CATGCAGAAC TACAGACTGA TTGGAGGCTC TGGAGGAAGC AGCGGCGGCA
     3181 GCTCGGGCGG TGGCGGCTCG AGCATCCTGT GGCATGAGAT GTGGCACGAG GGCCTGGAGG
     3241 AGGCCAGCAG GCTGTATTTC GGCGAGAGGA ATGTGAAGGG CATGTTCGAG GTGCTGGAGC
     3301 CCCTGCACGC CATGATGGAA AGGGGCCCAC AGACCCTGAA GGAAACTAGC TTCAACCAGG
     3361 CCTACGGCAG GGACCTGATG GAGGCCCAGG AGTGGTGCAG GAAGTACATG AAGAGCGGCA
     3421 ACGTGAAGGA TCTGACCCAG GCTTGGGACT TGTACTACCA CGTGTTCAGG AGGATCAGCT
     3481 CGGGCGGTGG CGGCTCGAGC ATCCTGTGGC ATGAGATGTG GCACGAGGGC CTGGAGGAGG
     3541 CCAGCAGGCT GTATTTCGGC GAGAGGAATG TGAAGGGCAT GTTCGAGGTG CTGGAGCCCC
     3601 TGCACGCCAT GATGGAAAGG GGCCCACAGA CCCTGAAGGA AACTAGCTTC AACCAGGCCT
     3661 ACGGCAGGGA CCTGATGGAG GCCCAGGAGT GGTGCAGGAA GTACATGAAG AGCGGCAACG
     3721 TGAAGGATCT GACCCAGGCT TGGGACTTGT ACTACCACGT GTTCAGGAGG ATCAGCTAAA
     3781 CTACGAACCC CGGGTAAATG CATGAATTCG ATCTAGAGGG CCCTATTCTA TAGTGTCACC
     3841 TAAATGCTAG AGCTCGCTGA TCAGCCTCGA CTGTGCCTTA CTAGGATCCT AGATAACTGA
     3901 TCTACCCAGC TTTCTTGTAC AAAGTGGTGA ATTCCGGCAA TTCGATATCA AGCTTATCGA
     3961 TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC
     4021 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG
     4081 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT
     4141 GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC
     4201 TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC
     4261 TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT
     4321 GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT
     4381 CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT
     4441 CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT
     4501 TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG
     4561 TCGACCTCGA GTCCTAGAAA AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA
     4621 TGCTGATTGT GCCTGGCTAG AAGCACAAGA GGAGGAGGAG GTGGGTTTTC CAGTCACACC
     4681 TCAGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA
     4741 AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATA TCCTTGATCT
     4801 GTGGATCTAC CACACACAAG GCTACTTCCC TGATTGGCAG AACTACACAC CAGGGCCAGG
     4861 GATCAGATAT CCACTGACCT TTGGATGGTG CTACAAGCTA GTACCAGTTG AGCAAGAGAA
     4921 GGTAGAAGAA GCCAATGAAG GAGAGAACAC CCGCTTGTTA CACCCTGTGA GCCTGCATGG
     4981 GATGGATGAC CCGGAGAGAG AAGTATTAGA GTGGAGGTTT GACAGCCGCC TAGCATTTCA
     5041 TCACATGGCC CGAGAGCTGC ATCCGGACTG TACTGGGTGT CTCTGGTTAG ACCAGATCTG
     5101 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC
     5161 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT
     5221 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGGGCC CGTTTAAACC CGCTGATCAG
     5281 CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT
     5341 TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC
     5401 ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG
     5461 AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG
     5521 CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA
     5581 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC
     5641 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG
     5701 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA
     5761 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC
     5821 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA
     5881 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT
     5941 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT
     6001 GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT
     6061 GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG
     6121 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT
     6181 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
     6241 TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG
     6301 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG
     6361 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC
     6421 GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG
     6481 CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC
     6541 GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC
     6601 CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG
     6661 CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC
     6721 ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC
     6781 AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC
     6841 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG
     6901 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA
     6961 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
     7021 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA
     7081 CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT
     7141 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG
     7201 GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG
     7261 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
     7321 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
     7381 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
     7441 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
     7501 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
     7561 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
     7621 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
     7681 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
     7741 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
     7801 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
     7861 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
     7921 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
     7981 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
     8041 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
     8101 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     8161 CGTTAAGGGA TTTTGGTCAT GACCAGCCAG GACAGAAATG CCTCGACTTC GCTGCTACCC
     8221 AAGGTTGCCG GGTGACGCAC ACCGTGGAAA CGGATGAAGG CACGAACCCA GTGGACATAA
     8281 GCCTGTTCGG TTCGTAAGCT GTAATGCAAG TAGCGTATGC GCTCACGCAA CTGGTCCAGA
     8341 ACCTTGACCG AACGCAGCGG TGGTAACGGC GCAGTGGCGG TTTTCATGGC TTGTTATGAC
     8401 TGTTTTTTTG GGGTACAGTC TATGCCTCGG GCATCCAAGC AGCAAGCGCG TTACGCCGTG
     8461 GGTCGATGTT TGATGTTATG GAGCAGCAAC GATGTTACGC AGCAGGGCAG TCGCCCTAAA
     8521 ACAAAGTTAA ACATTATGAG GGAAGCGGTG ATCGCCGAAG TATCGACTCA ACTATCAGAG
     8581 GTAGTTGGCG TCATCGAGCG CCATCTCGAA CCGACGTTGC TGGCCGTACA TTTGTACGGC
     8641 TCCGCAGTGG ATGGCGGCCT GAAGCCACAC AGTGATATTG ATTTGCTGGT TACGGTGACC
     8701 GTAAGGCTTG ATGAAACAAC GCGGCGAGCT TTGATCAACG ACCTTTTGGA AACTTCGGCT
     8761 TCCCCTGGAG AGAGCGAGAT TCTCCGCGCT GTAGAAGTCA CCATTGTTGT GCACGACGAC
     8821 ATCATTCCGT GGCGTTATCC AGCTAAGCGC GAACTGCAAT TTGGAGAATG GCAGCGCAAT
     8881 GACATTCTTG CAGGTATCTT CGAGCCAGCC ACGATCGACA TTGATCTGGC TATCTTGCTG
     8941 ACAAAAGCAA GAGAACATAG CGTTGCCTTG GTAGGTCCAG CGGCGGAGGA ACTCTTTGAT
     9001 CCGGTTCCTG AACAGGATCT ATTTGAGGCG CTAAATGAAA CCTTAACGCT ATGGAACTCG
     9061 CCGCCCGACT GGGCTGGCGA TGAGCGAAAT GTAGTGCTTA CGTTGTCCCG CATTTGGTAC
     9121 AGCGCAGTAA CCGGCAAAAT CGCGCCGAAG GATGTCGCTG CCGACTGGGC AATGGAGCGC
     9181 CTGCCGGCCC AGTATCAGCC CGTCATACTT GAAGCTAGAC AGGCTTATCT TGGACAAGAA
     9241 GAAGATCGCT TGGCCTCGCG CGCAGATCAG TTGGAAGAAT TTGTCCACTA CGTGAAAGGC
     9301 GAGATCACCA AGGTAGTCGG CAAATAATCA TGAGCGGATA CATATTTGAA TGTATTTAGA
     9361 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG
     9421 GATCGGGAGA TCTCCCGATC CCCTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC
     9481 ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG
     9541 CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT TGCATGAAGA ATCTGCTTAG
     9601 GGTTAGGCGT TTTGCGCTGC TTCGCGATGT ACGGGCCAGA TATACGCGTT GACATTGATT
     9661 ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA
     9721 GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG
     9781 CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG
     9841 ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA
     9901 TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC
     9961 CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC
    10021 TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC
    10081 ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA
    10141 TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG
    10201 GCGTGTACGG TGGGAGGTCT ATATAAGCAG CGCGTTTTGC
//