LOCUS       Exported                6802 bp ds-DNA     circular SYN 21-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 25, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5001..5933
     misc_feature    complement(23..187)
                     /label=ITR
                     /note="ITR"
     misc_feature    complement(194..236)
                     /label=seq
                     /note="seq"
     misc_feature    complement(251..270)
                     /label=pzac2.1 5088f
                     /note="pzac2.1 5088f"
     primer_bind     complement(351..374)
                     /label=f.ITR for 2.01.14
                     /note="f.ITR for 2.01.14"
     primer_bind     complement(452..472)
                     /label=f.ITR for 1.01.13
                     /note="f.ITR for 1.01.13"
     primer_bind     2471..2489
                     /label=r.ITR rev 3.01.19
                     /note="r.ITR rev 3.01.19"
     primer_bind     2513..2536
                     /label=r.ITR rev 4.01.20
                     /note="r.ITR rev 4.01.20"
     misc_feature    complement(2707..2871)
                     /label=ITR
                     /note="ITR"
     promoter        2898..3832
                     /label=CAG
                     /note="CAG "
     primer_bind     2975..2994
                     /label=f.CAG promoter seq.33.1743
                     /note="f.CAG promoter seq.33.1743"
     primer_bind     complement(3043..3058)
                     /label=r.CAG-flex ITR seq.33.1306 CAG rev
                     /note="r.CAG-flex ITR seq.33.1306 CAG rev"
     primer_bind     3049..3066
                     /label=f.CAG promoter.33.1744
                     /note="f.CAG promoter.33.1744"
     primer_bind     3483..3499
                     /label=f.CAG promoter seq.33.1747
                     /note="f.CAG promoter seq.33.1747"
     primer_bind     3495..3516
                     /label=r.CAG promoter seq.33.1745
                     /note="r.CAG promoter seq.33.1745"
     primer_bind     complement(3581..3603)
                     /label=r.CAG promoter seq.33.1746
                     /note="r.CAG promoter seq.33.1746"
     primer_bind     3703..3725
                     /label=f.CAG for.01.40
                     /note="f.CAG for.01.40"
     primer_bind     complement(3795..3818)
                     /label=r.CAG promoter seq.33.1748
                     /note="r.CAG promoter seq.33.1748"
     misc_feature    3833
                     /label=pCAG_GCaMP3
     misc_feature    3840..3845
                     /label=Kozak
     CDS             3846..3857
                     /codon_start=1
                     /label=MCS
                     /note="MCS"
                     /translation="MGRS"
     CDS             3858..4184
                     /codon_start=1
                     /label=PS3 epsilon
                     /note="PS3 epsilon"
                     /translation="MKTIHVSVVTPDGPVYEDDVEMVSVKAKSGELGILPGHIPLKAPL
                     EISAARLKKGGKTQYIAVSGGNLEVRPDKVTIYAQAAERAEDIDVLRAKAAKERAERRL
                     QSQVL"
     misc_feature    3882..3884
                     /label=T9V
                     /note="T9V"
     misc_feature    4035..4037
                     /label=Y60Y
                     /note="Y60Y"
     misc_feature    4089..4091
                     /label=N78Y
                     /note="N78Y"
     misc_feature    4140..4142
                     /label=A95K
     misc_feature    4179..4184
                     /label=QD>VL
                     /note="QD>VL"
     CDS             4185..4460
                     /codon_start=1
                     /label=cpsfGFP
                     /note="cpsfGFP"
                     /translation="VGNVYITADKQKNGIKANFKIRHNVEDGSMQLADHYQQNTPIGDG
                     PVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    4185..4190
                     /label=SG>VG
                     /note="SH>VG"
     misc_feature    4272..4274
                     /label=V>M
                     /note="V->M"
     CDS             4461..4478
                     /codon_start=1
                     /label=cpLinker
                     /note="cpsfGFP"
                     /translation="GGTGGS"
     CDS             4479..4913
                     /codon_start=1
                     /label=cpSFGFP
                     /note="cpSFGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNF"
     CDS             4914..4922
                     /codon_start=1
                     /label=L2-ICV
                     /note="L2+++"
                     /translation="ICV"
     CDS             4923..4994
                     /codon_start=1
                     /label=PS3 epsilon
                     /note="PS3 epsilon"
                     /translation="DIDFKRAELLLKRAMNRLSVAEMK"
     misc_feature    4950..4952
                     /label=A119
     CDS             4995..5000
                     /codon_start=1
                     /label=MCS
                     /translation="LQ"
     CDS             5001..5042
                     /codon_start=1
                     /label=3xGGGGS
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Commonly used flexible linker for
                     fusing two proteins."
                     /note="/full_name=3 tandem GGGGS"
                     /note="/name=3xGGGGS"
                     /note="/official_designation=3xGGGGS"
                     /note="/uuid=82e342ab8d8e4d71a1f1c7026d54944d"
                     /note="/vntifkey=21"
                     /translation="GGGSGGGGSGGGGS"
     CDS             5043..5933
                     /codon_start=1
                     /label=HaloTag
                     /label=Halo tag/SV40 NLS
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Halo tag/SV40 NLS"
                     /note="/official_designation=Halo tag/SV40 NLS"
                     /note="/uuid=3b230e3a699845e5874505cf15bc074d"
                     /note="/vntifkey=21"
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRTIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNVKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     primer_bind     5799..5808
                     /label=f.CAG promoter.33.1744
                     /note="f.CAG promoter.33.1744"
     misc_feature    5949..6537
                     /label=WPRE
                     /note="WPRE"
     primer_bind     complement(6020..6039)
                     /label=r.wpre rev.01.3
                     /note="r.wpre rev.01.3"
     primer_bind     complement(6350..6370)
                     /label=wpre rev pcr 32.63
                     /note="wpre rev pcr 32.63"
     primer_bind     6374..6393
                     /label=f.WPRE seq.01.80
                     /note="f.WPRE seq.01.80"
     primer_bind     6581..6604
                     /label=f.ITR for 3.01.15
                     /note="f.ITR for 3.01.15"
ORIGIN
        1 ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca
       61 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
      121 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
      181 gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      241 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      301 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      361 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      421 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      601 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      841 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1081 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1141 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1201 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1261 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1321 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1381 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1441 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1501 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1561 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1621 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1681 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1741 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1801 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1861 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1921 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1981 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2041 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2101 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2161 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     2221 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     2281 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     2341 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     2401 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     2461 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     2521 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     2581 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     2641 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     2701 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
     2761 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
     2821 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
     2881 tctaggaaga tcgtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2941 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3001 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3061 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3121 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     3181 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     3241 gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
     3301 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     3361 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     3421 gcgggagtcg ctgcgacgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     3481 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     3541 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     3601 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggctgtc
     3661 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     3721 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     3781 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttggatcccg
     3841 ccaccatggg cagatctatg aagactattc acgtgagtgt cgtaactccc gacgggcctg
     3901 tatatgagga tgacgttgaa atggtgagcg tcaaagcaaa aagtggcgag ctcggtattc
     3961 tcccaggcca cattcccttg aaagctcccc tagagatcag tgccgcacgc ctgaagaaag
     4021 ggggcaaaac acagtatatc gctgtgtcag gcggcaacct cgaagtgcgg cctgacaagg
     4081 tgaccatcta tgcccaggca gccgagaggg ctgaggatat cgatgtcctg cgcgccaaga
     4141 aagccaagga gagagccgag cgccgactcc aatcacaggt cctggtcggg aacgtctata
     4201 tcaccgccga caagcagaag aacggcatca aggcgaactt caagatccgc cacaacgtgg
     4261 aggacggcag catgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     4321 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgtgctgagc aaagacccta
     4381 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     4441 gcatggacga gctgtacaag ggcggtaccg gagggagcat gagcaagggc gaggagctgt
     4501 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4561 gcgtgcgcgg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct
     4621 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     4681 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4741 tgcccgaagg ctacgtccag gagcgcacca tcagcttcaa ggacgacggc acctacaaga
     4801 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4861 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tttatttgtg
     4921 tggacatcga ttttaagcgc gccgagctcg cccttaagcg cgcaatgaat aggctctcag
     4981 ttgccgaaat gaagctgcag ggcggaggct cgggcggagg tgggtcgggt ggcggcggat
     5041 caatggccga gatcggcacc ggcttcccct tcgaccccca ctacgtggag gtgctgggcg
     5101 agcgcatgca ctacgttgac gtgggccccc gcgacggcac ccccgtgctg ttcctgcacg
     5161 gcaaccccac cagcagctac gtgtggcgca acatcatccc ccacgtggcc cccacccacc
     5221 gcactatcgc ccccgacctg atcggcatgg gcaagagcga caagcccgac ctgggctact
     5281 tcttcgacga ccacgtgcgc ttcatggacg ccttcatcga ggccctgggc ctggaggagg
     5341 tggtgctggt gatccacgac tggggcagcg ccctgggctt ccactgggcc aagcgcaacc
     5401 ccgagcgcgt gaagggcatc gccttcatgg agttcatccg ccccatcccc acctgggacg
     5461 agtggcccga gttcgcccgc gagaccttcc aggccttccg caccaccgac gtgggccgca
     5521 agctgatcat cgaccagaac gtgttcatcg agggcaccct gcccatgggc gtggtgcgcc
     5581 ccctgaccga ggtggagatg gaccactacc gcgagccctt cctgaacccc gtggaccgcg
     5641 agcccctgtg gcgcttcccc aacgagctgc ccatcgccgg cgagcccgcc aacatcgtgg
     5701 ccctggtgga ggagtacatg gactggctgc accagagccc cgtgcccaag ctgctgttct
     5761 ggggcacccc cggcgtgctg atcccccccg ccgaggccgc ccgcctggcc aagagcctgc
     5821 ccaacgttaa ggccgtggac atcggccccg gcctgaacct gctccaggag gacaaccccg
     5881 acctgatcgg cagcgagatc gcccgctggc tgagcaccct cgagatcagc ggctagaagc
     5941 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6001 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6061 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6121 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6181 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6241 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6301 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6361 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6421 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6481 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     6541 gataccgtcg acccgggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     6601 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     6661 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     6721 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
     6781 acaaatgtgg taaaatcgat aa
//
