LOCUS       Exported               10875 bp ds-DNA     circular SYN 09-MAY-2023
DEFINITION  fck arch gfp (10,752 bp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2023 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10875
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5057..5098
     enhancer        245..624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     336..353
                     /label=f.CAG promoter.33.1744
                     /note="f.CAG promoter.33.1744"
     primer_bind     529..546
                     /label=f.CAG promoter.33.1744
                     /note="f.CAG promoter.33.1744"
     promoter        625..828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             842..1022
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1069..1194
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1687..1920
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2447..2564
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2609..3898
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     3878..3938
                     /label=ATPsnifer2 BamHI Kozak HiFi F
     misc_feature    3908..3913
                     /label=Kozak
     CDS             3914..4240
                     /codon_start=1
                     /label=PS3 epsilon
                     /note="PS3 epsilon"
                     /translation="MKTIHVSVVTPDGPVYEDDVEMVSVKAKSGELGILPGHIPLKAPL
                     EISAARLKKGGKTQYIAVSGGNLEVRPDKVTIYAQAAERAEDIDVLRAKAAKERAERRL
                     QSQVL"
     misc_feature    3938..3940
                     /label=T9V
                     /note="T9V"
     misc_feature    4091..4093
                     /label=Y60Y
                     /note="Y60Y"
     misc_feature    4145..4147
                     /label=N78Y
                     /note="N78Y"
     misc_feature    4196..4198
                     /label=A95K
     misc_feature    4235..4240
                     /label=QD>VL
                     /note="QD>VL"
     CDS             4241..4516
                     /codon_start=1
                     /label=cpsfGFP
                     /note="cpsfGFP"
                     /translation="VGNVYITADKQKNGIKANFKIRHNVEDGSMQLADHYQQNTPIGDG
                     PVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    4241..4246
                     /label=SG>VG
                     /note="SH>VG"
     misc_feature    4328..4330
                     /label=V>M
                     /note="V->M"
     CDS             4517..4534
                     /codon_start=1
                     /label=cpLinker
                     /note="cpsfGFP"
                     /translation="GGTGGS"
     CDS             4535..4969
                     /codon_start=1
                     /label=cpSFGFP
                     /note="cpSFGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNF"
     primer_bind     4585..4595
                     /label=miRFP670nano3 Stop EcoRI HiFi R
     CDS             4970..4978
                     /codon_start=1
                     /label=L2-ICV
                     /note="L2+++"
                     /translation="ICV"
     CDS             4979..5050
                     /codon_start=1
                     /label=PS3 epsilon
                     /note="PS3 epsilon"
                     /translation="DIDFKRAELLLKRAMNRLSVAEMK"
     misc_feature    5006..5008
                     /label=A119L
     CDS             5051..5056
                     /codon_start=1
                     /label=MCS
                     /translation="LQ"
     CDS             5057..5098
                     /codon_start=1
                     /label=3xGGGGS
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Commonly used flexible linker for
                     fusing two proteins."
                     /note="/full_name=3 tandem GGGGS"
                     /note="/name=3xGGGGS"
                     /note="/official_designation=3xGGGGS"
                     /note="/uuid=82e342ab8d8e4d71a1f1c7026d54944d"
                     /note="/vntifkey=21"
                     /translation="GGGSGGGGSGGGGS"
     primer_bind     complement(5083..5111)
                     /label=ATPsnifer2 HiFi R
     primer_bind     5087..5119
                     /label=miRFP670nano3 HiFi F
     CDS             5099..5539
                     /codon_start=1
                     /label=miRFP670nano3
                     /translation="MANLDKMLNTTVTEVRKFLQADRVCVFKFEEDYSGTVSHEAVDDR
                     WISILKTQVQDRYFMETRGEEYVHGRYQAIADIYTANLVECYRDLLIEFQVRAILAVPI
                     LQGKKLWGLLVAHQLAGPREWQTWEIDFLKQQAVVMGIAIQQS"
     primer_bind     complement(5524..5572)
                     /label=miRFP670nano3 Stop EcoRI HiFi R
     misc_feature    5567..6155
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6038..6049)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6158..6174)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6680..6860
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6892..7116
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7162..7590
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7604..7933
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7784..7919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7981..8028
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8047..8421
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8551..8672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8721..8737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8745..8761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8769..8799)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8814..8835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9123..9711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9882..10742)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10743..10847)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 athrsncgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
       61 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
      121 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
      181 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
      241 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      301 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      361 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      421 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      481 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      541 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      601 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      661 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      721 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      781 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac
      841 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
      901 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
      961 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     1021 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     1081 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     1141 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     1201 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     1261 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     1321 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     1381 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     1441 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     1501 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     1561 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1621 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1681 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1741 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1801 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1861 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     1921 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     1981 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     2041 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     2101 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     2161 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     2221 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     2281 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     2341 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     2401 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2461 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2521 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2581 acagcagaga tccagtttgg ttaattaaca ttatggcctt aggtcacttc atctccatgg
     2641 ggttcttctt ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc
     2701 tgcccagggt cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa
     2761 attccatgcc ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag
     2821 atgaccaatg agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca
     2881 gccctgggtg agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt
     2941 gacgggaagc acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc
     3001 ctctgggagg atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca
     3061 gtggtgacag ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt
     3121 ttggtacttc ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg
     3181 agagctggaa agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag
     3241 gaagcaaaga aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt
     3301 cccctgtaaa ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca
     3361 gaggagatga agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg
     3421 tggcacccac tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt
     3481 ctgttaaaag ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg
     3541 gactaagttt gttcacatcc ccttctccaa ccccctcagt acatcaccct gggagaacaa
     3601 ggtccacttg cttctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa
     3661 cggaggagca ggttttgaag tgaaaggcag gcaggtgttg gggaggcagt taccggggca
     3721 acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga cgaaggctcg
     3781 cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca atcaagctgg
     3841 ttctccattt gcactcagga gcacgggcag gcgagtggcc cctagttctg ggggcagcgg
     3901 gggatccgcc accatgaaga ctattcacgt gagtgtcgta actcccgacg ggcctgtata
     3961 tgaggatgac gttgaaatgg tgagcgtcaa agcaaaaagt ggcgagctcg gtattctccc
     4021 aggccacatt cccttgaaag ctcccctaga gatcagtgcc gcacgcctga agaaaggggg
     4081 caaaacacag tatatcgctg tgtcaggcgg caacctcgaa gtgcggcctg acaaggtgac
     4141 catctatgcc caggcagccg agagggctga ggatatcgat gtcctgcgcg ccaaggccgc
     4201 caaggagaga gccgagcgcc gactccaatc acaggtcctg gtcgggaacg tctatatcac
     4261 cgccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca acgtggagga
     4321 cggcagcatg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4381 gctgctgccc gacaaccact acctgagcac ccagtccgtg ctgagcaaag accctaacga
     4441 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4501 ggacgagctg tacaagggcg gtaccggagg gagcatgagc aagggcgagg agctgttcac
     4561 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4621 gcgcggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac
     4681 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     4741 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4801 cgaaggctac gtccaggagc gcaccatcag cttcaaggac gacggcacct acaagacccg
     4861 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4921 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttta tttgtgtgga
     4981 catcgatttt aagcgcgccg agctcttgct taagcgcgca atgaataggc tctcagttgc
     5041 cgaaatgaag ctgcagggcg gaggctcggg cggaggtggg tcgggtggcg gcggatcaat
     5101 ggcaaacctg gacaagatgc tgaacaccac cgtgaccgag gtgcgcaagt tcctgcaagc
     5161 ggacagagtg tgcgtgttca agttcgagga agattactcc ggcaccgtct cgcacgaagc
     5221 cgtggacgac agatggatta gcatcctgaa gacccaggtg caggacagat acttcatgga
     5281 aaccagaggc gaggaatacg tccacggcag ataccaggcc atcgccgaca tctacacagc
     5341 caatctggtc gagtgctaca gagacctgct gatcgagttt caggtgcggg ccattctggc
     5401 tgtccccatc ctgcaaggca agaagctgtg gggcctgctg gtggcccacc aactggccgg
     5461 ccctcgggag tggcagacct gggaaatcga cttcctgaaa cagcaagccg tggtgatggg
     5521 catcgccatc cagcagagct aggaattcga tatcaagctt atcgataatc aacctctgga
     5581 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5641 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5701 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5761 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5821 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5881 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5941 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     6001 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6061 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6121 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct
     6181 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     6241 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     6301 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6361 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     6421 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     6481 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     6541 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     6601 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     6661 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6721 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6781 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6841 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     6901 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6961 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     7021 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     7081 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     7141 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7201 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7261 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7321 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7381 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7441 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7501 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7561 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     7621 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7681 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7741 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7801 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7861 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7921 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     7981 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     8041 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     8101 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     8161 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     8221 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     8281 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     8341 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     8401 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
     8461 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8521 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8581 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8641 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     8701 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8761 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8821 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8881 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8941 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9001 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9061 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9121 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9181 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9241 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9301 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9361 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9421 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9481 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9541 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9601 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9661 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9721 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9781 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9841 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9901 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9961 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10021 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10081 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10141 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10201 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10261 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10321 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10381 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10441 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10501 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10561 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10621 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10681 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10741 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10801 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10861 aaagtgccac ctgac
//
