LOCUS       Exported               12283 bp ds-DNA     circular SYN 13-SEP-2022
DEFINITION  Syn-iATPSnFr-Halo 12283bp ds-DNA circular SYN 19-AUG-2022.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2023 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12283
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..821
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3891
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3896..3905
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3902..4822
                     /codon_start=1
                     /label=Synaptophysin
                     /translation="MDVVNQLVAGGQFRVVKEPLGFVKVLQWVFAIFAFATCGSYTGEL
                     RLSVECANKTESALNIEVEFEYPFRLHQVYFDAPSCVKGGTTKIFLVGDYSSSAEFFVT
                     VAVFAFLYSMGALATYIFLQNKYRENNKGPMMDFLATAVFAFMWLVSSSAWAKGLSDVK
                     MATDPENIIKEMPMCRQTGNTCKELRDPVTSGLNTSVVFGFLNLVLWVGNLWFVFKETG
                     WAAPFMRAPPGAPEKQPAPGDAYGDAGYGQGPGGYGPQDSYGPQGGYQPDYGQPASGGG
                     GYGPQGDYGQQGYGQQGAPTSFSNQM"
     CDS             4823..4870
                     /codon_start=1
                     /label=Linker
                     /translation="GSTSNGRQCAGILQIS"
     CDS             4871..4882
                     /codon_start=1
                     /label=MCS
                     /note="MCS"
                     /translation="MGRS"
     CDS             4883..5209
                     /codon_start=1
                     /label=PS3 epsilon
                     /note="PS3 epsilon"
                     /translation="MKTIHVSVVTPDGPVYEDDVEMVSVKAKSGELGILPGHIPLKAPL
                     EISAARLKKGGKTQYIAVSGGNLEVRPDKVTIYAQAAERAEDIDVLRAKAAKERAERRL
                     QSQVL"
     misc_feature    4907..4909
                     /label=T9V
                     /note="T9V"
     misc_feature    5060..5062
                     /label=Y60Y
                     /note="Y60Y"
     misc_feature    5114..5116
                     /label=N78Y
                     /note="N78Y"
     misc_feature    5159..5173
                     /label=AKKAK - STRONG AFFINITY
     misc_feature    5159..5173
                     /label=AKAAK -WEAK AFFINITY
     misc_feature    5165..5167
                     /label=A95K
     misc_feature    5204..5209
                     /label=QD>VL
                     /note="QD>VL"
     CDS             5210..5485
                     /codon_start=1
                     /label=cpsfGFP
                     /note="cpsfGFP"
                     /translation="VGNVYITADKQKNGIKANFKIRHNVEDGSMQLADHYQQNTPIGDG
                     PVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    5210..5215
                     /label=SG>VG
                     /note="SH>VG"
     misc_feature    5297..5299
                     /label=V>M
                     /note="V->M"
     CDS             5486..5503
                     /codon_start=1
                     /label=cpLinker
                     /note="cpsfGFP"
                     /translation="GGTGGS"
     CDS             5504..5938
                     /codon_start=1
                     /label=cpSFGFP
                     /note="cpSFGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNF"
     CDS             5939..5947
                     /codon_start=1
                     /label=L2-GLH
                     /note="L2+++"
                     /translation="ICV"
     CDS             5948..6019
                     /codon_start=1
                     /label=PS3 epsilon
                     /note="PS3 epsilon"
                     /translation="DIDFKRAELLLKRAMNRLSVAEMK"
     misc_feature    5975..5977
                     /label=A119L
     CDS             6020..6025
                     /codon_start=1
                     /label=MCS
                     /translation="LQ"
     CDS             6026..6070
                     /codon_start=1
                     /label=linker
                     /note="linker"
                     /translation="STGSGNAVGQDTQER"
     CDS             6071..6952
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     misc_feature    6975..7563
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7446..7457)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7566..7582)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8088..8268
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8300..8524
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8570..8998
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9012..9341
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9192..9327
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9389..9436
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9455..9829
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9959..10080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10129..10145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10153..10169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10177..10207)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10222..10243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10531..11119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11290..12150)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12151..12255)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcaca tccccttctc caaccccctc agtacatcac cctgggagaa caaggtccac
     3601 ttgcttctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caacggagga
     3661 gcaggttttg aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa
     3721 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct
     3781 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcaatcaagc tggttctcca
     3841 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggccac
     3901 catggacgtg gtgaatcagc tggtggctgg gggtcagttc cgggtggtca aggagcccct
     3961 tggcttcgtg aaggtgctgc agtgggtctt tgccatcttc gcctttgcta cgtgtggcag
     4021 ctacaccggg gagcttcggc tgagcgtgga gtgtgccaac aagacggaga gtgccctcaa
     4081 catcgaagtt gaattcgagt accccttcag gctgcaccaa gtgtactttg atgcaccctc
     4141 ctgcgtcaaa gggggcacta ccaagatctt cctggttggg gactactcct cgtcggctga
     4201 attctttgtc accgtggctg tgtttgcctt cctctactcc atgggggccc tggccaccta
     4261 catcttcctg cagaacaagt accgagagaa caacaaaggg cctatgatgg actttctggc
     4321 tacagccgtg ttcgctttca tgtggctagt tagttcatca gcctgggcca aaggcctgtc
     4381 cgatgtgaag atggccacgg acccagagaa cattatcaag gagatgccca tgtgccgcca
     4441 gacagggaac acatgcaagg aactgaggga ccctgtgact tcaggactca acacctcagt
     4501 ggtgtttggc ttcctgaacc tggtgctctg ggttggcaac ttatggttcg tgttcaagga
     4561 gacaggctgg gcagccccat tcatgcgcgc acctccaggc gccccggaaa agcaaccagc
     4621 acctggcgat gcctacggcg atgcgggcta cgggcagggc cccggaggct atgggcccca
     4681 ggactcctac gggcctcagg gtggttatca acccgattac gggcagccag ccagcggtgg
     4741 cggtggctac gggcctcagg gcgactatgg gcagcaaggc tatggccaac agggtgcgcc
     4801 cacctccttc tccaatcaga tgggatccac tagtaacggc cgccagtgtg ctggaattct
     4861 gcagatatcg atgggcagat ctatgaagac tattcacgtg agtgtcgtaa ctcccgacgg
     4921 gcctgtatat gaggatgacg ttgaaatggt gagcgtcaaa gcaaaaagtg gcgagctcgg
     4981 tattctccca ggccacattc ccttgaaagc tcccctagag atcagtgccg cacgcctgaa
     5041 gaaagggggc aaaacacagt atatcgctgt gtcaggcggc aacctcgaag tgcggcctga
     5101 caaggtgacc atctatgccc aggcagccga gagggctgag gatatcgatg tcctgcgcgc
     5161 caaggccgcc aaggagagag ccgagcgccg actccaatca caggtcctgg tcgggaacgt
     5221 ctatatcacc gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa
     5281 cgtggaggac ggcagcatgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5341 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgtgc tgagcaaaga
     5401 ccctaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     5461 tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatgagca agggcgagga
     5521 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     5581 gttcagcgtg cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt
     5641 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     5701 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5761 cgccatgccc gaaggctacg tccaggagcg caccatcagc ttcaaggacg acggcaccta
     5821 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5881 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactttat
     5941 ttgtgtggac atcgatttta agcgcgccga gctcttgctt aagcgcgcaa tgaataggct
     6001 ctcagttgcc gaaatgaagc tgcagtctac cggctccgga aatgctgtgg gccaggacac
     6061 gcaggagcgt gaaatcggca caggcttccc tttcgaccct cactacgtgg aagtgctggg
     6121 cgagagaatg cactatgtgg acgtgggccc cagagatgga acccctgtgc tgtttctgca
     6181 cggcaaccct accagctctt acgtgtggcg gaacatcatc cctcacgtgg cccctacaca
     6241 cagatgtatc gcccctgacc tgatcggcat gggcaagtct gacaagcctg acctgggcta
     6301 cttcttcgac gaccacgtgc ggttcatgga cgccttcatc gaagccctcg gactggaaga
     6361 ggtggtgctg gtcatccacg attggggatc tgccctggga ttccactggg ccaagaggaa
     6421 ccctgagaga gtgaagggaa tcgccttcat ggagttcatc aggcccattc ctacctggga
     6481 cgagtggcct gagttcgcca gagagacatt ccaggccttc aggaccacag acgtgggcag
     6541 aaagctgatc atcgaccaga acgtgttcat cgagggcacc ctgcctatgg gcgttgtcag
     6601 accactgacc gaggtggaaa tggaccacta cagagagccc tttctgaacc ccgtggacag
     6661 ggaacctctg tggcggtttc ctaacgagct gcctatcgct ggcgagcccg ctaatatcgt
     6721 ggctctggtg gaagagtaca tggactggct gcatcagagc cccgtgccta agctgctgtt
     6781 ctggggaaca cctggcgtgc tgatccctcc tgctgaagct gctagactgg ccaagagcct
     6841 gcctaactgc aaggccgtgg atatcggccc tggactgaac ctgctgcaag aggacaaccc
     6901 cgatctgatc ggctccgaga tcgccagatg gctgagcacc ctggaaatct cttagcgata
     6961 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7021 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7081 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7141 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7201 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7261 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7321 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     7381 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7441 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7501 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7561 cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata
     7621 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     7681 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     7741 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7801 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     7861 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     7921 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     7981 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     8041 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     8101 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8161 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8221 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     8281 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     8341 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     8401 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     8461 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     8521 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     8581 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8641 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8701 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8761 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8821 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8881 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8941 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     9001 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9061 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     9121 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     9181 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     9241 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     9301 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     9361 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     9421 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     9481 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     9541 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     9601 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     9661 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     9721 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     9781 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     9841 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     9901 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     9961 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10021 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    10081 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
    10141 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    10201 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    10261 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10321 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10381 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10441 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10501 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10561 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10621 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10681 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10741 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10801 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10861 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10921 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10981 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11041 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    11101 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11161 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11221 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11281 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11341 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11401 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11461 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11521 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11581 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11641 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11701 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11761 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11821 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11881 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11941 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    12001 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    12061 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    12121 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    12181 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    12241 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
