LOCUS       pCAG_HA/FLAG_tag_muMASS 8003 bp ds-DNA     circular     24-MAY-2023
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. backbone from archon 2 Insert
            is rat codon opt. uMASS1 from IDT.  second cloning reaction used
            KLD to add on a codon optimized HA/FLAG tag for enhanced
            expression.
FEATURES             Location/Qualifiers
     source          1..8003
                     /label="source"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     enhancer        39..418
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        421..696
                     /label="chicken beta-actin promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     intron          697..1708
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     1632..1654
                     /label="pCAGGS-5"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     1716..1735
                     /label="pCAG-F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1752..1774)
                     /label="R ba primer gibson"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     regulatory      1769..1774
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     regulatory      1769..1774
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      1769..1774
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      1769..1774
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1769..1774
                     /label="Kozak"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(1769..1774)
                     /label="r KLD primer"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1769..1777
                     /label="Kozak"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1775..1846
                     /label="HA FLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1775..1846
                     /label="dMASS1_HA_FLAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MKTIIALSYIFCLVFADYKDDDDA"
     regulatory      1847..1849
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(1847..1849)
                     /label="2590-R Primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     gene            1847..3490
                     /label="mLight_variant_insert"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     source          1847..3892
                     /label="source"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             1847..3892
                     /label="Translation 1847-3892"
                     /translation="MDSSTGPGNTSDCSDPLAQASCSPAPGSWLNLSHVDGNQSDPCGLNRTGLGGNDSLCPQTGSPSMVTAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGTILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPILIITVCYGLMILRLKSVRLSSLDNVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMVDQLKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSTIEQQNSTRVRQNTREHPSTANTVDRTNHQLENLEAETAPLPGGGKSRITSEGEYIPLDQIDINVGAPAAAFCYENEVG*"
     misc_feature    2036..2131
                     /label="TM1-mu"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2153..2236
                     /label="TM2-mu"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2258..2353
                     /label="TM3-mu"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2258..2353
                     /label="Translation 2258-2353"
                     /translation="ILCKIVISIDYYNMFTSIFTLCTMSVDRYIAV"
     CDS             2327..2338
                     /label="Translation 2327-2338"
                     /translation="MSVD"
     CDS             2339..2348
                     /label="Translation 2339-2348"
                     /translation="RYI"
     CDS             2351..2386
                     /label="Translation 2351-2386"
                     /translation="VCHPVKALDFRT"
     misc_feature    2387..2461
                     /label="TM4"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2522..2617
                     /label="TM5-rMOR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             2636..2644
                     /label="Translation 2636-2644"
                     /translation="LSS"
     CDS             2645..2650
                     /label="Translation 2645-2650"
                     /translation="LD"
     Fluorophore     2651..3373
                     /label="cpGFP"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             2651..3373
                     /label="Translation 2651-3373"
                     /translation="NVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYN"
     misc_feature    2857..2878
                     /label="EGFP-C-For"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(2985..3005)
                     /label="EGFP-N"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             3374..3397
                     /label="Translation 3374-3397"
                     /translation="MVDQLKEK"
     misc_feature    3398..3496
                     /label="TM6-mu"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3398..3496
                     /label="Translation 3398-3496"
                     /translation="DRNLRRITRMVLVVVAVFIVCWTPIHIYVIIKA"
     primer_bind     3468..3487
                     /label="gene-specific primer: CCATCCACATCTACGTCATC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          3468..3490
                     /label="1873-Forward Backbone"
                     /note="sequence: ccatccacatctacgtcatcatc"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(3468..3490)
                     /label="gene-specific primer: GATGATGACGTAGATGTGGATGG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3468..3490)
                     /label="1653-cDNA Reverse"
                     /note="sequence: gatgatgacgtagatgtggatgg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3470..3472
                     /label="6.51"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3482..3484
                     /label="6.55"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     gene            3491..3892
                     /label="GP27"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3518..3601
                     /label="TM7-mu"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             3602..3892
                     /label="Translation 3602-3892"
                     /translation="DENFKRCFREFCIPTSSTIEQQNSTRVRQNTREHPSTANTVDRTNHQLENLEAETAPLPGGGKSRITSEGEYIPLDQIDINVGAPAAAFCYENEVG*"
     primer          3785..3808
                     /label="2591-BaF"
                     /note="sequence: GGTAAGAGCAGAATCACAAGCGAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     trafficking     3788..3847
                     /label="Kir2.1 trafficking sequence"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     trafficking     3866..3886
                     /label="ER export motif"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3893..3914
                     /label="baF"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3893..3928
                     /label="Translation 3893-3928"
                     /translation="CTSKAAPDSRPQ"
     intron          3929..4061
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     4044..4063
                     /label="pMT2-F"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    4070..4658
                     /label="WPRE"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(4123..4143)
                     /label="WPRE-R"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(4541..4552)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(4685..4702)
                     /label="BGH-rev"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(4685..4702)
                     /label="BGHR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     polyA_signal    4691..4802
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     polyA_site      5094..5148
                     /label="-beta-globin poly(A) signal"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(5147..5166)
                     /label="rbglobpA-R"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(5503..5523)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(5507..5523)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(5507..5523)
                     /label="M13R"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(5507..5523)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(5520..5542)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(5521..5540)
                     /label="M13-48REV"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_binding    complement(5529..5551)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     protein_bind    5531..5547
                     /label="lac operator"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(5555..5585)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     protein_bind    5600..5621
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        5680..5875
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     rep_origin      5726..5861
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     5788..5807
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     polyA_signal    complement(5881..6002)
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    5881..6015
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(5918..5937)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     5972..5991
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(6083..6100)
                     /label="L4440"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      complement(6236..6918)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(6254..6842)
                     /label="ori"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(6334..6353)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(7311..7688)
                     /label="ISS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     7635..7654
                     /label="Amp-R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(7873..7977)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        complement(7873..7977)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 gtcgacattg attattgact agagtcatcc tcatcgtcga cattgattat tgactagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg
      421 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
      481 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      541 cgcgcgccag gcggggcggg gcgggggcga gggcggggcg gggcgaggcg gagaggtgcg
      601 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      661 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc
      721 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      781 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
      841 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
      901 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
      961 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     1021 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg
     1081 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     1141 cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc
     1201 cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg
     1261 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     1321 gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     1381 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
     1441 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
     1501 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
     1561 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
     1621 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     1681 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
     1741 tcatcatttt ggcaaagaat tcggtaccgc caccatgaag acgatcatcg ccctgagcta
     1801 catcttctgc ctggtgttcg ccgactacaa ggacgatgat gacgccatgg acagcagcac
     1861 cggcccaggg aacaccagcg actgctcaga ccccttagct caggcaagtt gctccccagc
     1921 acctggctcc tggctcaact tgtcccacgt tgatggcaac cagtccgatc catgcggtct
     1981 gaaccgcacc gggcttggcg ggaacgacag cctgtgccct cagaccggca gcccttccat
     2041 ggtcacagcc attaccatca tggccctcta ctctatcgtg tgtgtagtgg gcctcttcgg
     2101 aaacttcctg gtcatgtatg tgattgtaag atacaccaaa atgaagactg ccaccaacat
     2161 ctacattttc aaccttgctc tggcagacgc cttagcgacc agtacactgc cctttcagag
     2221 tgtcaactac ctgatgggaa catggccctt cggaaccatc ctctgcaaga tcgtgatctc
     2281 aatagattac tacaacatgt tcaccagcat attcaccctc tgcaccatga gcgtggaccg
     2341 ctacattgct gtctgccacc caGTCaaagc cctggatttc cgtacccccc gaaatgccaa
     2401 aatcgtcaac gtctgcaact ggatcctctc ttctgccatc ggtctgcctg taatgttcat
     2461 ggcaaccacG aaatacaggc aggggtccat agattgcacc ctcacgttct cccacccaac
     2521 ctggtactgg gagaacctgc tcaaaatctg tgtctttatc ttcgctttca tcatgccgat
     2581 cctcatcatc actgtgtgtt acggcctgat gatcttacga ctcaagagcg ttcgcctgag
     2641 ctcactTGAt aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt
     2701 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa
     2761 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc
     2821 caaactttcg aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2881 cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat
     2941 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     3001 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg
     3061 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     3121 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     3181 gcacgacttc ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt
     3241 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     3301 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     3361 gctggagtac aacATGGTTg accaactgaa agaaaaggac aggaatctgc gcaggatcac
     3421 ccggatggtg ctggtggtcg tggctgtatt tatcgtctgc tggaccccca tccacatcta
     3481 cgtcatcatc aaagcgctga tcacgattcc agaaaccaca tttcagaccg tttcctggca
     3541 cttctgcatt gctttgggtt acacgaacag ctgcctgaat ccagttcttt acgccttcct
     3601 ggatgaaaac ttcaagcgat gcttcagaga gttctgcatc ccaacctcgt ccacgatcga
     3661 acagcaaaac tccactcgag tccgtcagaa cactagggaa catccctcca cggctaatac
     3721 agtggatcga actaaccacc agctagaaaa tctggaggca gaaactgctc cattgcccGG
     3781 CGGAGGTAAG AGCAGAATCA CAAGCGAGGG CGAGTACATC CCTCTGGACC AGATCGACAT
     3841 CAACGTGGGA GCACCAGCCG CCGCTTTCTG CTACGAGAAC GAAGTCGGCt aatgtacaag
     3901 taaagcggcc ccggactcga ggccgcaggt aagtatcaag gttacaagac aggtttaagg
     3961 agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     4021 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtcgaca atcaacctct
     4081 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4141 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4201 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4261 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4321 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4381 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4441 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     4501 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4561 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4621 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacga
     4681 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4741 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4801 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4861 ggggaggatt gggaagacaa tagcaggcca gcttttgttc cctttagggt taattgcgcg
     4921 cttggcgcta atcatggtca tagctgtttc cactcctcag gtgcaggctg cctatcagaa
     4981 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc gttttccctc
     5041 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     5101 gaaatttatt ttcattgcaa tagtgtgttg aattttttgt gtctctcact cggaaggaca
     5161 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     5221 tatgccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa
     5281 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     5341 tttttatatt ttgtttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     5401 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     5461 cttatgaaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc
     5521 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5581 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5641 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac
     5701 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5761 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5821 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5881 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5941 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6001 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6061 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6241 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6421 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     6481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6661 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7021 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7081 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7141 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7201 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7261 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7321 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7381 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7441 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7501 tggagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7561 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7621 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7681 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7741 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7801 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7861 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7921 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7981 atttccccga aaagtgccac ctg
//