LOCUS       Exported               13176 bp ds-DNA     circular SYN 26-SEP-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 27, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pInducer21_Puro        12048 bp    DNA     circular     
            6-NOV-2013

            SOURCE      

              ORGANISM  

            COMMENT     This file is created by Vector NTI

                        http://www.invitrogen.com/

            COMMENT     ORIGDB|GenBank

            COMMENT     VNTDATE|677942888|

            COMMENT     VNTDBDATE|677944473|

            COMMENT     LSOWNER|

            COMMENT     VNTNAME|pInducer21_Puro|

            COMMENT     VNTAUTHORNAME|Thomas|

            COMMENT     VNTOAUTHORNAME|Demo User|

            COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)

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            COMMENT                          ""Foreign object. Author: Thomas. 
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FEATURES             Location/Qualifiers
     source          1..13176
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     LTR             454..634
                     /label=HIV-1-3-LTR
                     /note="color: #00ffff; direction: BOTH"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    743..746
                     /label=SD
                     /note="color: #0080ff"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    1910..1912
                     /label=SA
                     /note="color: #0080ff"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    2062..2077
                     /label=cPPT
                     /note="color: #0080ff"
     promoter        2217..2650
                     /label=TRE2
                     /note="color: #ff8080"
     protein_bind    2230..2500
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
                     /note="This feature has 13 segments:
                         1: 2230 .. 2248 / #31849b / tetO
                         2: 2249 .. 2271 / #31849b
                         3: 2272 .. 2290 / #31849b / tetO
                         4: 2291 .. 2313 / #31849b
                         5: 2314 .. 2332 / #31849b / tetO
                         6: 2333 .. 2355 / #31849b
                         7: 2356 .. 2374 / #31849b / tetO
                         8: 2375 .. 2397 / #31849b
                         9: 2398 .. 2416 / #31849b / tetO
                        10: 2417 .. 2439 / #31849b
                        11: 2440 .. 2458 / #31849b / tetO
                        12: 2459 .. 2481 / #31849b
                        13: 2482 .. 2500 / #31849b / tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2591..2609
                     /label=O1578, pIND21fwd
                     /note="O1578, pIND21fwd"
                     /note="color: #50ff50"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     CDS             2731..5424
                     /codon_start=1
                     /label=human TAOK3 Cop
                     /note="STE20-like kinase; hKFC-A; JNK/SAPK-inhibitory 
                     kinase; jun kinase-inhibitory kinase; kinase from chicken 
                     homolog A; CTCL-associated antigen HD-CL-09; dendritic 
                     cell-derived protein kinase; thousand and one amino acid 
                     protein 3; cutaneous T-cell lymphoma-associated antigen 
                     HD-CL-09"
                     /note="color: #ff0000"
                     /translation="MRKGVLKDPEIADLFYKDDPEELFIGLHEIGHGSFGAVYFATNAH
                     TSEVVAIKKMSYSGKQTHEKWQDILKEVKFLRQLKHPNTIEYKGCYLKEHTAWLVMEYC
                     LGSASDLLEVHKKPLQEVEIAAITHGALHGLAYLHSHALIHRDIKAGNILLTEPGQVKL
                     ADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGITCIELAERKPPLF
                     NMNAMSALYHIAQNDSPTLQSNEWTDSFRRFVDYCLQKIPQERPTSAELLRHDFVRRDR
                     PLRVLIDLIQRTKDAVRELDNLQYRKMKKILFQETRNGPLNESQEDEEDSEHGTSLNRE
                     MDSLGSNHSIPSMSVSTGSQSSSVNSMQEVMDESSSELVMMHDDESTINSSSSVVHKKD
                     HVFIRDEAGHGDPRPEPRPTQSVQSQALHYRNRERFATIKSASLVTRQIHEHEQENELR
                     EQMSGYKRMRRQHQKQLIALENKLKAEMDEHRLKLQKEVETHANNSSIELEKLAKKQVA
                     IIEKEAKVAAADEKKFQQQILAQQKKDLTTFLESQKKQYKICKEKIKEEMNEDHSTPKK
                     EKQERISKHKENLQHTQAEEEAHLLTQQRLYYDKNCRFFKRKIMIKRHEVEQQNIREEL
                     NKKRTQKEMEHAMLIRHDESTRELEYRQLHTLQKLRMDLIRLQHQTELENQLEYNKRRE
                     RELHRKHVMELRQQPKNLKAMEMQIKKQFQDTCKVQTKQYKALKNHQLEVTPKNEHKTI
                     LKTLKDEQTRKLAILAEQYEQSINEMMASQALRLDEAQEAECQALRLQLQQEMELLNAY
                     QSKIKMQTEAQHERELQKLEQRVSLRRAHLEQKIEEELAALQKERSERIKNLLERQERE
                     IETFDMESLRMGFGNLVTLDFPKEDYR"
     CDS             2800..3561
                     /codon_start=1
                     /label=kinase domain
                     /note="kinase domain"
                     /note="color: #0080ff"
                     /translation="FIGLHEIGHGSFGAVYFATNAHTSEVVAIKKMSYSGKQTHEKWQD
                     ILKEVKFLRQLKHPNTIEYKGCYLKEHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAI
                     THGALHGLAYLHSHALIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMA
                     PEVILAMDEGQYDGKVDIWSLGITCIELAERKPPLFNMNAMSALYHIAQNDSPTLQSNE
                     WTDSFRRFVDYCLQKIPQERPTSAELLRHDFV"
     exon            2851..2922
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            2923..3024
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            3025..3070
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            3071..3167
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            3168..3281
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            3282..3373
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            3374..3467
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            3468..3549
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            3550..3717
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     misc_feature    3676..3678
                     /label=TAOK3
                     /note="Phosphothreonine; propagated from 
                     UniProtKB/Swiss-Prot (Q9H2K8.2); phosphorylation site"
                     /note="color: #0080ff"
     misc_feature    3700..3702
                     /label=TAOK3
                     /note="Phosphoserine, by ATM; propagated from 
                     UniProtKB/Swiss-Prot (Q9H2K8.2); phosphorylation site"
                     /note="color: #0080ff"
     exon            3718..3924
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     misc_feature    3721..3723
                     /label=TAOK3
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot 
                     (Q9H2K8.2); phosphorylation site"
                     /note="color: #0080ff"
     exon            3925..4059
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     misc_feature    4054..4056
                     /label=TAOK3
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot 
                     (Q9H2K8.2); phosphorylation site"
                     /note="color: #0080ff"
     exon            4060..4296
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            4297..4425
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            4426..4629
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            4630..4869
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            4870..5082
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     exon            5083..5265
                     /label=TAOK3
                     /note="color: #80ff80; direction: RIGHT"
     STS             5200..5339
                     /label=TAOK3
                     /note="color: #ff0000"
     protein_bind    complement(5426..5450)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: LEFT"
     CDS             5503..5568
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 5568"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5569..5579
                     /label=Stop codons
                     /note="Stop codons"
                     /note="color: #0080ff"
     CDS             5586..5612
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             5586..5612
                     /codon_start=1
                     /label=HA tag
                     /note="color: #0080ff"
                     /translation="YPYDVPDYA"
     misc_feature    complement(5677..5697)
                     /label=O1579, pIND21rev
                     /note="O1579, pIND21rev"
                     /note="color: #50ff50; direction: LEFT"
     promoter        5832..7010
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          6063..7001
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     protein_bind    complement(7080..7113)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     CDS             7120..7866
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /note="This feature has 8 segments:
                        1: 7120 .. 7737 / #993366 / rTetR
                        2: 7738 .. 7743 / #cc99b2
                        3: 7744 .. 7779 / #993366 / VP16 minimal transcriptio...
                        4: 7780 .. 7782 / #cc99b2
                        5: 7783 .. 7818 / #993366 / VP16 minimal transcriptio...
                        6: 7819 .. 7821 / #cc99b2
                        7: 7822 .. 7857 / #993366 / VP16 minimal transcriptio...
                        8: 7858 .. 7866 / #cc99b2"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7538..7558
                     /label=pind 21 fwd
                     /note="color: #0080ff"
     misc_feature    7919..8491
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     CDS             8524..9123
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9377..9397
                     /label=pind21 rev
                     /note="color: #0080ff"
     misc_feature    9566..10154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(10037..10048)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 10036"
                     /translation="IEGR"
     LTR             10229..10462
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             10282..10462
                     /label=HIV-1-5 LTR (LTR-SIN)
                     /note="color: #00ffff; direction: BOTH"
     promoter        10667..10771
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             10772..11632
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10772 .. 10840 / #ccffcc / signal sequence
                        2: 10841 .. 11632 / #ccffcc
                     Cleavage site after base 10840"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11787..12406
                     /direction=RIGHT
                     /label=pUC ori
                     /note="color: #c0c0c0"
     rep_origin      11803..12391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    12802..12823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        12838..12868
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 12838 .. 12843 / #ffffff / -35
                        2: 12844 .. 12861 / #ffffff
                        3: 12862 .. 12868 / #ffffff / -10"
     protein_bind    12876..12892
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     12900..12916
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctccacc atgcgtaaag gggtgctgaa ggacccagag
     2761 attgccgatc tattctacaa agatgatcct gaggaacttt ttattggttt gcatgaaatt
     2821 ggacatggaa gttttggagc agtttatttt gctacaaatg ctcacaccag tgaggtggtg
     2881 gcaattaaga agatgtccta tagtgggaag cagacccatg agaaatggca agatattctt
     2941 aaggaagtta aatttttacg acaattgaag catcctaata ctattgagta caaaggctgt
     3001 tacttgaaag aacacactgc ttggttggtg atggaatatt gcttaggctc agcctctgat
     3061 ttattagaag ttcataaaaa accacttcag gaagtggaga tcgctgccat tactcatgga
     3121 gccttgcatg gactagccta cctacattct catgcattga ttcataggga tattaaagca
     3181 ggaaatattc ttctaacaga gccaggtcag gtaaaactag ctgattttgg atctgcttca
     3241 atggcttctc ctgccaactc cttcgtgggc acaccttact ggatggctcc agaggtgatc
     3301 ttagctatgg atgaaggaca gtatgatggg aaagttgata tttggtcact tggcatcact
     3361 tgtattgaat tggcggaacg gaagccgccc cttttcaaca tgaatgcaat gagtgcctta
     3421 tatcacattg cccagaatga ctccccaacg ttacagtcta atgaatggac agactccttt
     3481 aggagatttg ttgattactg cttgcagaaa atacctcagg aaaggccaac atcagcagaa
     3541 ctattaaggc atgactttgt tcgacgagac cggccactac gtgtcctcat tgacctcata
     3601 cagaggacaa aagatgcagt tcgtgagcta gataacctac agtaccgaaa aatgaaaaaa
     3661 atacttttcc aagagacacg gaatggaccc ttgaatgagt cacaggagga tgaggaagac
     3721 agtgaacatg gaaccagcct gaacagggaa atggacagcc tgggcagcaa ccattccatt
     3781 ccaagcatgt ccgtgagcac aggcagccag agcagcagtg tgaacagcat gcaggaagtc
     3841 atggacgaga gcagttccga acttgtcatg atgcacgatg acgaaagcac aatcaattcc
     3901 agctcctccg tcgtgcataa gaaagatcat gtattcataa gggatgaggc gggccacggc
     3961 gatcccaggc ctgagccgcg gcctacccag tcagttcaga gccaggccct ccactaccgg
     4021 aacagagagc gctttgccac gatcaaatca gcatctttgg ttacacgaca gatccatgag
     4081 catgagcagg agaacgagtt gcgggaacag atgtcaggtt ataagcggat gcggcgccag
     4141 caccagaagc agctgatcgc cctggagaac aagctgaagg ctgagatgga cgagcaccgc
     4201 ctcaagctac agaaggaggt ggagacgcat gccaacaact cgtccatcga gctggagaag
     4261 ctggccaaga agcaagtggc tatcatagaa aaggaggcaa aggtagctgc agcagatgag
     4321 aagaagttcc agcaacagat cttggcccag cagaagaaag atttgacaac tttcttagaa
     4381 agtcagaaga agcagtataa gatttgtaag gaaaaaataa aagaggaaat gaatgaggac
     4441 catagcacac ccaagaaaga gaagcaagag cggatctcca aacataaaga gaacttgcag
     4501 cacacacagg ctgaagagga agcccacctt ctcactcaac agagactgta ctacgacaaa
     4561 aattgtcgtt tcttcaagcg gaaaataatg atcaagcggc acgaggtgga gcagcagaac
     4621 attcgggagg aactaaataa aaagaggacc cagaaggaga tggagcatgc catgctaatc
     4681 cggcacgacg agtccacccg agagctagag tacaggcagc tgcacacgtt acagaagcta
     4741 cgcatggatc tgatccgttt acagcaccag acggaactgg aaaaccagct ggagtacaat
     4801 aagaggcgag aaagagaact gcacagaaag catgtcatgg aacttcggca acagccaaaa
     4861 aacttaaagg ccatggaaat gcaaattaaa aaacagtttc aggacacttg caaagtacag
     4921 accaaacagt ataaagcact caagaatcac cagttggaag ttactccaaa gaatgagcac
     4981 aaaacaatct taaagacact gaaagatgag cagacaagaa aacttgccat tttggcagag
     5041 cagtatgaac agagtataaa tgaaatgatg gcctctcaag cgttacggct agatgaggct
     5101 caagaagcag aatgccaggc cttgaggcta cagctccagc aggaaatgga gctgctcaac
     5161 gcctaccaga gcaaaatcaa gatgcaaaca gaggcacaac atgaacgtga gctccagaag
     5221 ctagagcaga gagtgtctct gcgcagagca caccttgagc agaagattga agaggagctg
     5281 gctgcccttc agaaggaacg cagcgagaga ataaagaacc tattggaaag gcaagagcga
     5341 gagattgaaa cttttgacat ggagagcctc agaatgggat ttgggaattt ggttacatta
     5401 gattttccta aggaggacta cagagaccca gctttcttgt acaaagtggt tgatatccag
     5461 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aagactacaa agaccatgac
     5521 ggtgattata aagatcatga catcgattac aaggatgacg atgacaagta ggtaagtgat
     5581 tcgaataccc atacgacgtc ccagactacg cttagtaatg attaattaaa ctagaaattc
     5641 taccgggtag gggaggcgct tttcccaagg cagtctggag taacccaccc aagatctggc
     5701 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agccgcgtaa
     5761 gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag
     5821 cctagtcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     5881 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     5941 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     6001 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     6061 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     6121 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     6181 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     6241 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     6301 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     6361 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     6421 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     6481 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     6541 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     6601 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     6661 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     6721 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     6781 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     6841 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     6901 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     6961 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga agcggccgcc
     7021 atgctagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacggatcca
     7081 taacttcgta taatgtatgc tatacgaagt tatgccacca tgtctagact ggacaagagc
     7141 aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca
     7201 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     7261 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
     7321 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc
     7381 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     7441 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     7501 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     7561 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     7621 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     7681 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     7741 ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac
     7801 tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc
     7861 gggtaactaa gtaaggatcc gcggccgcac tagaggaatt ccgcccctct cccccccccc
     7921 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     7981 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     8041 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     8101 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     8161 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     8221 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     8281 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     8341 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     8401 catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga
     8461 cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccacaag gagacgacct
     8521 tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc
     8581 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg
     8641 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     8701 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     8761 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     8821 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     8881 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     8941 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     9001 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     9061 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     9121 tgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     9181 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     9241 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     9301 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     9361 tctatcgcct tcttgacgag ttcttctgat gtacaagtaa agcggccgcg actctagatc
     9421 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     9481 cccctgaacc tgaaacataa aatgaatgca attgttgttg tttagtccct cccaattcga
     9541 tatcaagctt atcgcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt
     9601 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     9661 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     9721 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     9781 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     9841 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     9901 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     9961 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
    10021 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
    10081 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
    10141 ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt
    10201 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
    10261 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    10321 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    10381 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    10441 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
    10501 cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga
    10561 tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
    10621 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
    10681 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    10741 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    10801 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    10861 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    10921 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    10981 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
    11041 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    11101 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    11161 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    11221 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    11281 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
    11341 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
    11401 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    11461 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
    11521 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    11581 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    11641 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    11701 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    11761 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    11821 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    11881 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    11941 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    12001 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    12061 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    12121 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    12181 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    12241 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    12301 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    12361 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    12421 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    12481 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    12541 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
    12601 cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat
    12661 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    12721 ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc
    12781 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    12841 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    12901 aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc
    12961 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    13021 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    13081 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
    13141 ttgtatttgt taaatatgta ctacaaactt agtagt
//
