LOCUS       HAND1-Tom_target        6935 bp DNA     circular SYN 03-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Birket lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2023 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6935
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     CDS             complement(146..396)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="LAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQL
                     RSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICSDAA"
     primer_bind     377..418
                     /label=Fragment 1.FOR
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            397..1199
                     /gene="HAND1"
                     /gene_synonym="bHLHa27; eHand; Hxt; Thing1"
                     /label=HAND1
                     /note="heart and neural crest derivatives expressed 1"
                     /db_xref="GeneID:9421"
                     /db_xref="HGNC:HGNC:4807"
                     /db_xref="MIM:602406"
     exon            1098..1199
                     /gene="HAND1"
                     /gene_synonym="bHLHa27; eHand; Hxt; Thing1"
                     /number=2
                     /inference="alignment:same species:2.1.0"
     CDS             1098..1199
                     /codon_start=1
                     /gene="HAND1"
                     /gene_synonym="bHLHa27; eHand; Hxt; Thing1"
                     /product="heart- and neural crest derivatives-expressed
                     protein 1"
                     /label=HAND1
                     /note="class A basic helix-loop-helix protein 27;
                     extraembryonic tissues, heart, autonomic nervous system and
                     neural crest derivatives-expressed protein 1"
                     /db_xref="CCDS:CCDS4327.1"
                     /db_xref="GeneID:9421"
                     /db_xref="HGNC:HGNC:4807"
                     /db_xref="MIM:602406"
                     /protein_id="NP_004812.1"
                     /translation="QQHEGFPPALGPVEKRIKGRTGWPQQVWALELNQ"
     mRNA            1098..1199
                     /gene="HAND1"
                     /gene_synonym="bHLHa27; eHand; Hxt; Thing1"
                     /product="heart and neural crest derivatives expressed 1"
                     /label=HAND1 mRNA
                     /db_xref="GeneID:9421"
                     /db_xref="HGNC:HGNC:4807"
                     /db_xref="MIM:602406"
                     /transcript_id="NM_004821.3"
     misc_feature    1098..1148
                     /gene="HAND1"
                     /gene_synonym="bHLHa27; eHand; Hxt; Thing1"
                     /note="propagated from UniProtKB/Swiss-Prot (O96004.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     primer_bind     complement(1180..1199)
                     /label=Backbone_REV
     primer_bind     complement(1181..1209)
                     /label=Fragment 1.REV
     misc_feature    1187..1199
                     /label=HAND1_gRNA1
     primer_bind     1190..1215
                     /label=Fragment 2.FOR
     misc_feature    complement(1199)
                     /label=HAND1_gRNA2
     CDS             1200..1253
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A - rare codons changed
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1252..1257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1254..1955
                     /label=dTOMATO
                     /label=tdTOMATO_reference-genbank_HQ418395_reference-genba 
                     nk_ADC42843
     primer_bind     1951..1972
                     /label=Backbone_FOR
     gene            1992..2025
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(1992..2025)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     gene            2084..2598
                     /label=PGK promoter
                     /note="PGK promoter"
     promoter        2090..2589
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     gene            2601..2666
                     /label=EM7 promoter
                     /note="EM7 promoter"
     promoter        2601..2648
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2667..3470
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2667..3467
                     /label=Neo
                     /note="Neo"
     gene            3484..3786
                     /label=bGHpA
                     /note="bGHpA"
     polyA_signal    3508..3732
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     gene            3789..3822
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(3789..3822)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(3832..3865)
                     /label=Fragment 2.REV
     primer_bind     complement(3838..3854)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3846..3871
                     /label=Fragment 3.FOR
     gene            complement(3854)
                     /gene="POLR2F"
                     /gene_synonym="HRBP14.4; POLRF; RPABC14.4; RPABC2;RPB14.4; 
                     RPB6; RPC15"
                     /label=POLR2F
                     /note="RNA polymerase II, I and III subunit F; Derived by 
                     automated computational analysis using gene prediction 
                     method: BestRefSeq."
                     /db_xref="GeneID:5435"
                     /db_xref="HGNC:HGNC:9193"
                     /db_xref="MIM:604414"
     gene            3854
                     /gene="SOX10"
                     /gene_synonym="DOM; PCWH; WS2E; WS4; WS4C"
                     /label=SOX10
                     /note="SRY-box transcription factor 10; Derived by
                     automated computational analysis using gene prediction 
                     method: BestRefSeq."
                     /db_xref="GeneID:6663"
                     /db_xref="HGNC:HGNC:11190"
                     /db_xref="MIM:602229"
     mRNA            3854
                     /gene="SOX10"
                     /gene_synonym="DOM; PCWH; WS2E; WS4; WS4C"
                     /product="SRY-box transcription factor 10"
                     /label=SOX10 mRNA
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq.
                     /transcript_id=NM_006941.4"
                     /db_xref="Ensembl:ENST00000396884.8"
                     /db_xref="GeneID:6663"
                     /db_xref="HGNC:HGNC:11190"
                     /db_xref="MIM:602229"
     misc_feature    3855
                     /label=3' HA
     misc_feature    3855
                     /label=gRNA 1
     misc_feature    complement(3855)
                     /label=gRNA 2
     exon            3856..4633
                     /gene="HAND1"
                     /gene_synonym="bHLHa27; eHand; Hxt; Thing1"
                     /number=2
                     /inference="alignment:same species:2.1.0"
     mRNA            3856..4633
                     /gene="HAND1"
                     /gene_synonym="bHLHa27; eHand; Hxt; Thing1"
                     /product="heart and neural crest derivatives expressed 1"
                     /label=HAND1 mRNA
                     /db_xref="GeneID:9421"
                     /db_xref="HGNC:HGNC:4807"
                     /db_xref="MIM:602406"
                     /transcript_id="NM_004821.3"
     gene            3856..4633
                     /gene="HAND1"
                     /gene_synonym="bHLHa27; eHand; Hxt; Thing1"
                     /label=HAND1
                     /note="heart and neural crest derivatives expressed 1"
                     /db_xref="GeneID:9421"
                     /db_xref="HGNC:HGNC:4807"
                     /db_xref="MIM:602406"
     misc_feature    3856..3865
                     /label=HAND1_gRNA1
     CDS             3856..3861
                     /codon_start=1
                     /gene="HAND1"
                     /gene_synonym="bHLHa27; eHand; Hxt; Thing1"
                     /product="heart- and neural crest derivatives-expressed
                     protein 1"
                     /label=HAND1
                     /note="class A basic helix-loop-helix protein 27;
                     extraembryonic tissues, heart, autonomic nervous system and
                     neural crest derivatives-expressed protein 1"
                     /db_xref="CCDS:CCDS4327.1"
                     /db_xref="GeneID:9421"
                     /db_xref="HGNC:HGNC:4807"
                     /db_xref="MIM:602406"
                     /protein_id="NP_004812.1"
                     /translation="Q"
     misc_feature    complement(3858..3877)
                     /label=HAND1_gRNA2
     primer_bind     complement(4611..4653)
                     /label=Fragment 3.REV
     CDS             complement(4634..4718)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHAGLCSRRARDPISRCIREV"
     primer_bind     complement(4714..4730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4738..4754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4762..4792)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4807..4828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5116..5704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5875..6735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6736..6840)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgtgac gaaagctcat ccagggacga
      421 gggaaagata aataggcatc gagtctctaa acggctgccc cggactgggg atggtgcagg
      481 ccggcttctt tcgaggtgcg gcgcctcgaa agaagttgtt attgggcgga tcccggcccc
      541 cacgcgtcgc cgcctccttc aagtagcacc gcttggttcc tgctgaaaga gcgggaagga
      601 ggacgctagg ccagctcgac tccccgctct tttacctccg tatctgaggc tcccagctcc
      661 ggcttagggg gccccaagcc tgcatccaag ccctgatgtt taaaccaggt tgggaggctg
      721 gcggaagtgt gtggggtgca gcgagagcgg gagaaccgtg gatttccacc cctcccttcc
      781 aatatcctgg gatctccctt aggtgtccta aagccaaatc aacgcgcgtc agtttcacca
      841 gacgcagaac attttcctta ccaaacctcc cgaagctgcc cagcccatcc cgggtcccag
      901 gaactccgcg cataaaggca aatttacgag cctgtttgcc tatttcgcaa acagaaatcc
      961 gtcacttatt taaagactcc cagttactgc tcccgacacc ttcccctcct cctcatctta
     1021 ccccatgccg ggcgatcgga gcagaaaaat taaaacctgt ctctcccgcc tcttttcttc
     1081 tgtgtgtcct ttggcagcag cagcacgaag gttttcctcc tgccctgggc ccagtcgaga
     1141 agaggattaa aggacgcacc ggctggccgc agcaagtctg ggcgctggag ttaaaccagg
     1201 agggcagggg aagcctgctg acatgcgggg acgtggagga aaatcccggc cccatggtga
     1261 gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca
     1321 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     1381 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     1441 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt
     1501 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1561 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg
     1621 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg
     1681 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     1741 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca
     1801 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca
     1861 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc
     1921 acctgttcct gtacggcatg gacgagctgt acaagtgagc ccaattccga tcatattcaa
     1981 taacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt ctgaagagga
     2041 gtttacgtcc agccaagcta gcttggctgc aggtcgtcga aattctaccg ggtaggggag
     2101 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     2161 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     2221 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     2281 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     2341 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     2401 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     2461 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     2521 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     2581 ggcctttcga cctgcagcct gttgacaatt aatcatcggc atagtatatc ggcatagtat
     2641 aatacgacaa ggtgaggaac taaaccatgg gatcggccat tgaacaagat ggattgcacg
     2701 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     2761 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     2821 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     2881 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     2941 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3001 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3061 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     3121 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     3181 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg
     3241 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     3301 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     3361 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     3421 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatcaat
     3481 tctctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     3541 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     3601 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     3661 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     3721 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgactaga gcttgcggaa
     3781 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgaggggat
     3841 ccactagttc tagatcagtg agccgaggcc cgcgccgagg acctggccag gccagccact
     3901 cctgaagccc cgggaggaga ggaaggcagc ggcgaacgcc aggctctggg ctccggcgac
     3961 tggtgctacg catcccgcgg agcttctgct gagcgccggc aggtcgtcgg ctgcaaccac
     4021 acacttggat cgcacgtgca atgtcctttg atttttttta atacattaag agaaagagaa
     4081 atatatatat atccaccccc agcccaaccg agggcggccc ttggcggcaa catgcaagaa
     4141 ggagggactg tcgaacccaa gggctcaaag acgcactctt ccaccctttt ggagcgaatt
     4201 tagaacctca gccctatctc catttcccta tctggctctt tctctcttgt ccctccatat
     4261 gatccgcccc gacgccgtct tctctaatta aaatgcaata aggaatcaat tcttttctgc
     4321 ctgagaaaga gaaccagacg caggaagatg aaaggctgcc ctttgttctt cgaatcgtgg
     4381 tggttttatt ttatttttct ttttgtcgct gcacttcctg tttagttcca agggaaacac
     4441 tttctctctt tctctgtctc tctcttttct tccttctttt ccttcctttt tgtttctatc
     4501 taaataaaag ctttccctgt gttggaaagt ttttatgtat ttaaactacc taccatgcct
     4561 gttgtgctca ggtgtttgtt catcctgcca tccccaaccc ttttctacct caagtctgtg
     4621 tgaccactca caggtacctc gcgaatgcat ctagatatcg gatcccgggc ccgtcgactg
     4681 cagaggcctg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4741 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4801 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4861 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4921 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4981 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5041 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5101 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5161 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5221 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5281 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5341 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5401 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5461 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5521 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     5581 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5641 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5701 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5761 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5821 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5881 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5941 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6001 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6061 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6121 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6181 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6241 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6301 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6361 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6421 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6481 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6541 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6601 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6661 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6721 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6781 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6841 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6901 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
