LOCUS       Exported                6977 bp ds-DNA     circular SYN 21-JUL-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Radboudumc
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 2, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     681..692
                     /label=pEGFP?CMV
                     /note="color: black; sequence: TAACAACTCCGCCCCATT"
     primer_bind     731..748
                     /label=VP1.5 seq primer
                     /note="color: black; sequence: GGACTTTCCAAAATGTCG"
     primer_bind     749..766
                     /label=pEGFP?CMV
                     /note="color: black; sequence: TAACAACTCCGCCCCATT"
     primer_bind     791..814
                     /label=cmv-cloning
                     /note="color: black; sequence: ACGGTGGGAGGTCTATATAAGCAG"
     primer_bind     863..882
                     /label=T7 primer
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     intron          1646..1875
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
                     /note="color: #------"
     primer_bind     1866..1884
                     /label=pCIFW2
                     /note="color: black; sequence: CTCTCCACAGGTGTCCACT"
     misc_feature    1933..2506
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     primer_bind     2496..2514
                     /label=RUSHfw2
                     /note="color: black; sequence: CACGATGATAAGCTTGCCA"
     CDS             2534..3627
                     /codon_start=1
                     /label=STX5L M55V
                     /note="color: #993366"
                     /translation="MIPRKRYGSKNTDQGVYLGLSKTQVLSPATAGSSSSDIAPLPPPV
                     TLVPPPPDTVSCRDRTQEFLSACKSLQTRQNGIQTNKPALRAVRQRSEFTLMAKRIGKD
                     LSNTFAKLEKLTILAKRKSLFDDKAVEIEELTYIIKQDINSLNKQIAQLQDFVRAKGSQ
                     SGRHLQTHSNTIVVSLQSKLASMSNDFKSVLEVRTENLKQQRSRREQFSRAPVSALPLA
                     PNHLGGGAVVLGAESHASKDVAIDMMDSRTSQQLQLIDEQDSYIQSRADTMQNIESTIV
                     ELGSIFQQLAHMVKEQEETIQRIDENVLGAQLDVEAAHSEILKYFQSVTSNRWLMVKIF
                     LILIVFFIIFVVFLARGGSGGSTN"
     primer_bind     2534..2574
                     /label=STX5deltaER4
                     /note="color: black; sequence: 
                     cacgcactGGCGCGCCtaccATGATTCCTGCAGCTGCATACGGGTCTAAGAACACGGAT
                     CA"
     primer_bind     2546..2567
                     /label=QPCR_STX5fw3
                     /note="color: black; sequence: AAACGCTACGGGTCTAAGAACA"
     primer_bind     2552..2574
                     /label=STX5Ldelta ER3
                     /note="color: black; sequence: 
                     cacgcactGGCGCGCCtaccATGGCCCCTGCAGCTGCATACGGGTCTAAGAACACGGAT
                     CA"
     primer_bind     complement(2774..2793)
                     /label=QPCR_STX5rev3
                     /note="color: black; sequence: GCACGCAAAGCTGGCTTATT"
     primer_bind     2862..2881
                     /label=QPCR_STX5fw5
                     /note="color: black; sequence: CCAAGCTGGAGAAGCTGACA"
     primer_bind     2890..2910
                     /label=QPCR_STX5fw1
                     /note="color: black; sequence: AAAGCGCAAGTCCCTCTTTGA"
     primer_bind     complement(2967..2988)
                     /label=QPCR_STX5rev1
                     /note="color: black; sequence: TGAGCAATTTGTTTGTTGAGGC"
     primer_bind     complement(2983..3002)
                     /label=QPCR_STX5rev5
                     /note="color: black; sequence: CGAAATCCTGGAGCTGAGCA"
     primer_bind     3251..3269
                     /label=QPCR_STX5fw2
                     /note="color: black; sequence: TCCAAGGATGTCGCCATCG"
     primer_bind     complement(3322..3341)
                     /label=QPCR_STX5rev2
                     /note="color: black; sequence: CCCGACTCTGGATGTAGGAA"
     primer_bind     3382..3401
                     /label=QPCR_STX5fw4
                     /note="color: black; sequence: GGGCTCCATCTTTCAGCAGT"
     primer_bind     complement(3483..3502)
                     /label=QPCR_STX5rev4
                     /note="color: black; sequence: GAGGATCTCTGAATGGGCGG"
     CDS             3629..3739
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /note="color: #cc99b2"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     3650..3673
                     /label=RUSHfw1
                     /note="color: black; sequence: CGAGGTGGACACGTTGTTGAAGGA"
     primer_bind     complement(3691..3712)
                     /label=RUSHrev1
                     /note="color: black; sequence: CTCCAGTCGTGCACGAAGTTGT"
     CDS             3752..4468
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 2 segments:
                        1: 3752 .. 3754 / #e1ff2f / 1a
                        2: 3755 .. 4468 / #e1ff2f"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3788..3808)
                     /label=pEGFP?rev
                     /note="color: black; sequence: GTCCAGCTCGACCAGGATGGG"
     primer_bind     complement(3877..3906)
                     /label=citseq1
                     /note="color: black; sequence: 
                     TTGCCGGTGGTGCAGATGAACTTCAGGGTC"
     primer_bind     4172..4199
                     /label=citseq2
                     /note="color: black; sequence: 
                     CTGGAGTACAACTACAACAGCCACAACG"
     primer_bind     complement(4175..4193)
                     /label=mCitrmutR
                     /note="color: black; sequence: GGCTGTTGTAGTTGTACTC"
     primer_bind     4194..4214
                     /label=mCitrmutF
                     /note="color: black; sequence: ACAACGTGTAtATCATGGCCG"
     primer_bind     4427..4440
                     /label=pEGFP?c
                     /note="color: black; sequence: GCCGCCGGGATCAC"
     primer_bind     complement(4500..4531)
                     /label=RUSH-pHluorin rev1
                     /note="color: black; sequence: 
                     Caagtaaaacctctacaaatgtggtatggctg"
     primer_bind     complement(4502..4522)
                     /label=pEGFP-all
                     /note="color: black; sequence: CCTCTACAAATGTGGTATGGC"
     primer_bind     complement(4541..4563)
                     /label=RUSH-pHluorin rev2
                     /note="color: black; sequence: Gttcagggggaggtgtgggaggt"
     primer_bind     complement(4544..4563)
                     /label=pEGFP-all2
                     /note="color: black; sequence: GTTCAGGGGGAGGTGTGGGA"
     primer_bind     complement(4577..4602)
                     /label=RUSHrev2
                     /note="color: black; sequence: CAAGTTAACAACAACAATTGCATTCA"
     polyA_signal    4597..4718
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     4677..4698
                     /label=pCI-rev
                     /note="color: black; sequence: GCATTCTAGTTGTGGTTTGTCC"
     primer_bind     4695..4717
                     /label=pCIREV2
                     /note="color: black; sequence: GTCCAAACTCATCAATGTATCTT"
     primer_bind     4730..4751
                     /label=pCI-rev
                     /note="color: black; sequence: GCATTCTAGTTGTGGTTTGTCC"
     primer_bind     4748..4770
                     /label=pCIREV2
                     /note="color: black; sequence: GTCCAAACTCATCAATGTATCTT"
     primer_bind     complement(4816..4836)
                     /label=M13-REV
                     /note="color: black; sequence: CAGGAAACAGCTATGACCATG"
     primer_bind     complement(4819..4840)
                     /label=pGEMPR1
                     /note="color: black; sequence: Cacacaggaaacagctatgacc"
     primer_bind     complement(4820..4840)
                     /label=M13 rev
                     /note="color: black; sequence: CACACAGGAAACAGCTATGAC"
     primer_bind     complement(4820..4836)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(4820..4836)
                     /label=M13_pDONR201_rev
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     protein_bind    4844..4860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(4868..4898)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 4868 .. 4874 / #ffffff / -10
                        2: 4875 .. 4892 / #ffffff
                        3: 4893 .. 4898 / #ffffff / -35"
     protein_bind    4913..4934
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(5222..5810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(5981..6841)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5981 .. 6772 / #ccffcc
                        2: 6773 .. 6841 / #ccffcc / signal sequence
                     Cleavage site after base 6772"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6842..6946)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     6886..6903
                     /label=EF1aseqfw1
                     /note="color: black; sequence: GTCTCATGAGCGGATACA"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cgatatctgc ggcctagcta gcgcttaagg cctgttaacc ggtatggatg
      961 tatgcgtccg tcttgccctg tggctcctct ggggactcct cctgcaccag ggccagagcc
     1021 tcagccatag tcacagtgag aaggcgacag gaaccagctc gggcgccggt ttaaacgacc
     1081 ctagcaaaga ctcaaaagct caggtgtccg ctgccgaggc tggcattact ggaacatggt
     1141 acaatcagct cgggagcacc tttattgtga ctgctggagc cgatggagcc ctcaccggaa
     1201 catacgaatc tgctgtggga aacgccgaat cacggtacgt cctcactggc cgatacgata
     1261 gtgcccctgc caccgacgga tctgggactg ccctgggatg gactgtcgct tggaaaaaca
     1321 actaccggaa tgctcattct gccacaacat ggagtggaca gtacgtggga ggcgctgagg
     1381 ctagaatcaa tacacagtgg ctgctcacat ctggcacaac cgaggcaaat gcttggaaat
     1441 ccaccctggt gggacatgac acattcacca aagtgaaacc ctccgccgct tcaatcgatg
     1501 ccgccaaaaa agccggagtc aacaacggca atcctctgga tgccgtccag cagctcggac
     1561 cgaaagatga actgtgagcg gccgcataga taactgatcc agtgtgctgg aattaattcg
     1621 ctgtctgcga gggccagctg ttggggtgag tactccctct caaaagcggg catgacttct
     1681 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg
     1741 atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc
     1801 ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg
     1861 cctttctctc cacaggtgtc cactcccagg tccaactgca ggtcgagcat gcatctaggg
     1921 cggccaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     1981 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     2041 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     2101 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     2161 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     2221 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     2281 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     2341 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     2401 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     2461 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc
     2521 gggaggcgcg ccaatgatcc cgcggaaacg ctacgggtct aagaacacgg atcagggtgt
     2581 ctacctgggt ctctcaaaga cacaggtcct gtcccctgca actgctggca gtagcagcag
     2641 cgacatcgcc cctctgcccc ccccagtgac cctcgtccct ccccctcccg acaccgtgtc
     2701 ctgccgggat cggacccagg agtttctgtc tgcctgcaag tcgctgcaga cccgtcagaa
     2761 tggaatccag acaaataagc cagctttgcg tgctgtccga caacgcagtg agttcaccct
     2821 catggccaag cgcattggga aagaccttag caacacattt gccaagctgg agaagctgac
     2881 aatcttggca aagcgcaagt ccctctttga tgataaagca gtggaaattg aagagctaac
     2941 atatatcatc aaacaggaca tcaatagcct caacaaacaa attgctcagc tccaggattt
     3001 cgtgagagcc aagggcagcc agagtggccg gcacctgcag acccactcca acaccattgt
     3061 ggtctccttg cagtcgaaac tggcttctat gtccaatgac ttcaaatcgg ttttagaagt
     3121 gaggacagag aacctgaagc agcagaggag ccggagagag cagttctccc gggcacctgt
     3181 gtcagccctg ccccttgccc ctaaccacct gggcggtggt gctgtggttc tgggggcaga
     3241 gtcccatgcc tccaaggatg tcgccatcga catgatggac tctcggacca gccagcagct
     3301 gcagctcatt gacgagcagg attcctacat ccagagtcgg gcagacacca tgcagaacat
     3361 tgagtcgaca attgttgagt tgggctccat ctttcagcag ttggcacaca tggttaagga
     3421 acaggaggaa accattcaga ggatcgacga gaacgtgcta ggagcccagc tggacgttga
     3481 ggccgcccat tcagagatcc tcaagtactt ccagtctgtc acctccaacc ggtggctcat
     3541 ggtcaaaatc ttcctcatcc tcattgtctt cttcatcatc tttgtggtct tccttgctcg
     3601 gggcggcagc ggcggcagca cgaattccga cgagaagacc actggttggc gaggtggaca
     3661 cgttgttgaa ggactggctg gggaacttga acaacttcgt gcacgactgg agcatcaccc
     3721 acaaggtcaa cgtgaaccac ctgcaggtgc tgtgagcaag ggcgaggagc tgttcaccgg
     3781 cgtggtgccc atcctggtgg agctggacgg cgacgtgaac ggccacaagt tcagcgtgag
     3841 cggcgagggc gagggcgacg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3901 cggcaagctg cccgtgccct ggcccaccct ggtgaccacc ttcggctacg gcctgatgtg
     3961 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagagcg ccatgcccga
     4021 gggctacgtg caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4081 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4141 caaggaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca gccacaacgt
     4201 gtacatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     4261 catcgaggac ggcagcgtgc agctggccga ccactaccag cagaacaccc ccatcggcga
     4321 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagagcaagc tgagcaagga
     4381 ccccaacgag aagcgcgacc acatggtgct gctggagttc gtgaccgccg ccggcatcac
     4441 cctgggcatg gacgagctgt acaagtaagc ggccgcgact ctagataact gatcataatc
     4501 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     4561 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     4621 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4681 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtcgagtg cattctagtt
     4741 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     4801 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4861 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     4921 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4981 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5041 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5101 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5161 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5221 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5281 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5341 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5401 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5461 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5521 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5581 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5641 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     5701 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5761 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5821 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5881 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5941 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6001 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6061 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6121 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6181 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6241 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6301 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6361 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6421 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6481 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6541 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6601 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6661 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6721 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6781 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6841 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6901 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6961 aagtgccacc tgacgtc
//
