LOCUS       Exported                4748 bp ds-DNA     circular SYN 09-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 29, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        16..319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        320..523
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(524..545)
                     /label=SplitFluo Subclone  Vector R Primer
     primer_bind     526..563
                     /label=SplitFluo Subclone  F Primer
     regulatory      557..566
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             575..1267
                     /codon_start=1
                     /label=GA
                     /translation="MVSKSEEVIKEFMRFKVRLEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPLIMYGSKMYVKHPADVPDYMKLSFPEGFKWERVMHFEDGG
                     LVTATQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTLGWDYATERLYPEEGVLKGELL
                     GRLKLKDGGLNLVESKTIYMAKKPVQLPGYYFVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLGHGTGST"
     primer_bind     1233..1242
                     /label=SplitFluo Subclone  F Primer
     CDS             1268..1303
                     /codon_start=1
                     /label=NES
                     /translation="LALKLAGLDIGS"
     primer_bind     complement(1313..1355)
                     /label=SplitFluo Subclone  R Primer
     primer_bind     1336..1353
                     /label=HP Vector F Primer
     polyA_signal    1491..1612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1619..2074)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2101..2205
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2207..2564
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2415..2550
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2599..3393
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3625..3672
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4001..4589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
       61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      541 gatccgctag cgtgccgcca ccatggcctc gagaatggtg agcaagagcg aggaggtcat
      601 caaagagttc atgcgcttca aggtgcgctt ggagggctcc atgaacggcc acgagttcga
      661 gatcgagggc gagggcgagg gccgccctta cgagggcacc cagaccgcca agctgaaggt
      721 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tcccccctga tcatgtacgg
      781 ctccaagatg tacgtgaagc accccgccga cgtccccgat tacatgaagc tgtccttccc
      841 cgagggcttc aagtgggagc gcgtgatgca cttcgaggac ggcggtctgg tgaccgcgac
      901 acaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
      961 cttccccccc gacggccccg taatgcagaa gaagaccttg ggctgggatt atgccaccga
     1021 gcgcctgtac cccgaagaag gcgtgctgaa gggcgagctc ctggggcgcc tgaagctgaa
     1081 ggacggcggc ctcaacctgg tggagtccaa gaccatctac atggccaaga agcccgtgca
     1141 actgcccggc tactacttcg tggacaccaa gctggacatc acctcccaca acgaggacta
     1201 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctggggc atggcaccgg
     1261 cagcacctta gccttgaaat tagcaggtct tgatatcggg agctaaaagc ttggtaaacc
     1321 gctgatcagc ctcgactgca gtcgacggta ccgcgggccc gggatccacc ggatctagat
     1381 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     1441 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     1501 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     1561 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
     1621 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     1681 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     1741 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     1801 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     1861 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     1921 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     1981 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     2041 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     2101 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2161 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     2221 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     2281 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2341 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     2401 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2461 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2521 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     2581 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     2641 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     2701 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     2761 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     2821 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     2881 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     2941 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     3001 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     3061 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     3121 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     3181 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     3241 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     3301 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     3361 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     3421 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     3481 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     3541 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     3601 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     3661 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     3721 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     3781 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     3841 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3901 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3961 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4021 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4081 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4141 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4201 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4261 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4321 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4381 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4441 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4501 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4561 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4621 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4681 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
     4741 catagccc
//
