LOCUS       pA-CBh.TP-CNOT8-V5H6.MC 6721 bp ds-DNA     linear       10-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    22..281
                     /label="CMV Early Enhancer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    22..819
                     /label="CBh chimeric Promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    309..628
                     /label="Chicken b-actin promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    498..502
                     /label="CAAT box"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="RNA polymerase binding site/origin of transcription"
     misc_feature    517..525
                     /label="CArG box"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="cis regulatory element"
     misc_feature    560..566
                     /label="TATA box"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="cis regulatory element"
     misc_feature    629..679
                     /label="C b-actin exon1 (5'UTR)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    680..722
                     /label="CBa intron chimera"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    728..813
                     /label="MVM VP intron chimera"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    814..842
                     /label="MVM splice site (5'UTR)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    843..849
                     /label="kozak sequence"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    850..1240
                     /label="MS2 Coat Protein"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             850..2226
                     /label="Translation 850-2226"
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIYGGASSMPAALVENSQVICEVWASNLEEEMRKIREIVLSYSYIAMDTEFPGVVVRPIGEFRSSIDYQYQLLRCNVDLLKIIQLGLTFTNEKGEYPSGINTWQFNFKFNLTEDMYSQDSIDLLANSGLQFQKHEEEGIDTLHFAELLMTSGVVLCDNVKWLSFHSGYDFGYMVKLLTDSRLPEEEHEFFHILNLFFPSIYDVKYLMKSCKNLKGGLQEVADQLDLQRIGRQHQAGSDSLLTGMAFFRMKELFFEDSIDDAKYCGRLYGLGTGVAQKQNEDVDSAQEKMSILAIINNMQQRDPGPRFEGKPIPNPLLGLDSTRTGHHHHHH*"
     misc_feature    1252..2132
                     /label="CNOT8"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2155..2196
                     /label="V5 Epitope"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2206..2223
                     /label="Hisx6 Tag"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2271..2492
                     /label="SV40 polyA signal"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2390..2395
                     /label="A2UA3"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2496..3083
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3115..3822
                     /label="mCherry Fluor"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             3115..4422
                     /label="Translation 3115-4422"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKMTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3823..4422
                     /label="Puromycin Resistance"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4466..4690
                     /label="BGH PolyA Element"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4886..5474
                     /label="pUC Origin of Replication"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5061..5061
                     /label="G to A mutation"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Does not affect plasmid yield."
     misc_feature    5645..6505
                     /label="Ampicillin Resistance"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(5645..6505)
                     /label="Translation 6505-5645"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
ORIGIN
        1 CAACTTTGTA TAGAAAAGTT GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC
       61 GCCCAACGAC CCCCGCCCAT TGACGTCAAT AGTAACGCCA ATAGGGACTT TCCATTGACG
      121 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
      181 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTGTGCCCA
      241 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
      301 TACCATGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC CCCCCTCCCC
      361 ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG GGGCGGGGGG
      421 GGGGGGGGGG CGCGCGCCAG GCGGGGCGGG GCGGGGCGAG GGGCGGGGCG GGGCGAGGCG
      481 GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT TTATGGCGAG
      541 GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG
      601 CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT
      661 GACCGCGTTA CTCCCACAGG TGAGCGGGCG GGACGGCCCT TCTCCTCCGG GCTGTAATTA
      721 GCTGAGCAAG AGGTAAGGGT TTAAGGGATG GTTGGTTGGT GGGGTATTAA TGTTTAATTA
      781 CCTGGAGCAC CTGCCTGAAA TCACTTTTTT TCAGGTTGGC AAGTTTGTAC AAAAAAGCAG
      841 GCTGCCACCA TGGCTTCTAA CTTTACTCAG TTCGTTCTCG TCGACAATGG CGGAACTGGC
      901 GACGTGACTG TCGCCCCAAG CAACTTCGCT AACGGGGTCG CTGAATGGAT CAGCTCTAAC
      961 TCGCGTTCAC AGGCTTACAA AGTAACCTGT AGCGTTCGTC AGAGCTCTGC GCAGAATCGC
     1021 AAATACACCA TCAAAGTCGA GGTGCCTAAA GTGGCAACCC AGACTGTTGG TGGTGTAGAG
     1081 CTTCCTGTAG CCGCATGGCG TTCGTACTTA AATATGGAAC TAACCATTCC AATTTTCGCT
     1141 ACGAATTCCG ACTGCGAGCT TATTGTTAAG GCAATGCAAG GTCTCCTAAA AGATGGAAAC
     1201 CCGATTCCCT CGGCCATCGC AGCAAACTCC GGCATCTACG GCGGCGCTAG CAGCATGCCC
     1261 GCTGCCCTGG TTGAGAATTC CCAGGTGATC TGCGAAGTGT GGGCATCCAA TCTTGAAGAG
     1321 GAGATGCGAA AGATTCGGGA AATCGTATTG TCCTATAGTT ACATAGCGAT GGATACAGAA
     1381 TTCCCCGGAG TCGTTGTGCG GCCCATTGGT GAATTTCGGA GCTCTATCGA CTATCAGTAT
     1441 CAGCTCCTGA GGTGTAATGT GGACCTTTTG AAGATCATTC AACTGGGACT GACTTTCACA
     1501 AACGAGAAAG GCGAATATCC CTCAGGAATA AATACCTGGC AATTTAATTT CAAATTCAAT
     1561 CTGACAGAGG ATATGTATAG CCAAGACTCC ATAGATCTCC TTGCGAACTC TGGCCTCCAG
     1621 TTTCAGAAAC ACGAAGAGGA AGGTATCGAC ACCCTGCACT TTGCCGAACT GCTTATGACC
     1681 TCTGGAGTTG TGCTTTGTGA TAACGTTAAA TGGCTGTCTT TCCACAGTGG TTACGATTTC
     1741 GGTTACATGG TAAAGCTTTT GACTGATTCC CGATTGCCTG AGGAAGAGCA TGAGTTCTTC
     1801 CACATTCTCA ATCTTTTCTT TCCGTCCATT TATGACGTGA AGTACCTGAT GAAAAGCTGC
     1861 AAGAACTTGA AGGGGGGTTT GCAGGAGGTA GCTGACCAAT TGGATTTGCA AAGAATCGGT
     1921 AGGCAACACC AGGCTGGTTC CGACTCCTTG CTTACGGGAA TGGCGTTCTT TAGAATGAAG
     1981 GAACTCTTCT TTGAGGACAG CATTGATGAT GCTAAGTATT GCGGTAGGCT TTACGGTCTT
     2041 GGAACAGGTG TAGCGCAGAA GCAAAATGAG GATGTAGACA GCGCTCAAGA GAAAATGAGT
     2101 ATTTTGGCAA TAATCAATAA CATGCAGCAA AGGGATCCGG GCCCGCGGTT CGAAGGTAAG
     2161 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGCGTA CCGGTCATCA TCACCATCAC
     2221 CATTGAACCC AGCTTTCTTG TACAAAGTGG TGATGGCCGG CCGCTTCGAG CAGACATGAT
     2281 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT
     2341 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT
     2401 TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT
     2461 TTAAAGCAAG TAAAACCTCT ACAAATGTGG TACGCGTTGA CATTGATTAT TGACTAGTTA
     2521 TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC
     2581 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC
     2641 AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT
     2701 GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC
     2761 GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC
     2821 CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT
     2881 GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC
     2941 AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT
     3001 TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG
     3061 GGAGGTCTAT ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCGC CACCATGGTG
     3121 AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCAC
     3181 ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC
     3241 TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCGCC
     3301 TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC
     3361 GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG
     3421 AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG
     3481 TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG
     3541 AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG
     3601 AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC
     3661 AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA CGTCAACATC
     3721 AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC
     3781 GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA AGATGACCGA GTACAAGCCC
     3841 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG
     3901 TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC
     3961 ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC
     4021 GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG
     4081 GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG
     4141 CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC
     4201 ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC
     4261 GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC
     4321 AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA
     4381 GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACTCGAGTC TAGAGGGCCC
     4441 GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC
     4501 CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA
     4561 AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG
     4621 GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG
     4681 GGCTCTATGG GCGGCCGCGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG
     4741 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG
     4801 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC
     4861 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
     4921 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
     4981 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
     5041 TGTCCGCCTT TCTCTCTTCG TGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
     5101 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
     5161 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
     5221 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
     5281 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA
     5341 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
     5401 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     5461 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
     5521 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
     5581 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
     5641 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
     5701 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
     5761 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA
     5821 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
     5881 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
     5941 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
     6001 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
     6061 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
     6121 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
     6181 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
     6241 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
     6301 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
     6361 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
     6421 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
     6481 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
     6541 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
     6601 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA
     6661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCGGCGC GCCGCGGCCG
     6721 C
//