LOCUS       pNL3.2.CMV.3UTR-TH.NLuc 5641 bp ds-DNA     circular     10-AUG-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    14..755
                     /label="CMV immediate early enhancer/promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    720..726
                     /label="TATA box"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    734..747
                     /label="crs"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="cis-repression sequence"
     misc_feature    748..748
                     /label=+1 
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="transcription start site"
     misc_feature    748..755
                     /label="inr"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="initiator-like sequence"
     misc_feature    853..858
                     /label="Kozac"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    859..1371
                     /label="NanoLuc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             859..1497
                     /label="Translation 859-1497"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILANSHGFPPEVEEQAADTLPMSCAQESGMDRHPAACASARINV*"
     misc_feature    1375..1494
                     /label="PEST sequence from Mm ornithine decarboxylase"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(1500..1516)
                     /label="For invPCR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1517..1547
                     /label="CW101 addition"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1539..1557
                     /label="Target Hairpin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1548..1577
                     /label="CW 100 addition"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1578..1594
                     /label="For invPCR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1587..1808
                     /label="SV40 late poly(A) region"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1706..1711
                     /label="A2UA3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1856..2274
                     /label="SV40 early enhancer/promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2293..2298
                     /label="Kozac"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             2299..3336
                     /label="Translation 2299-3336"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKEVGRV*"
     misc_feature    2299..3336
                     /label="Hygromycin resistance gene"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3360..3408
                     /label="Synthetic Poly-(A) tail"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3732..3768
                     /label="Origin Element"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4523..5383
                     /label="Beta-lactamse (Amp resistance)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(4523..5383)
                     /label="Translation 5383-4523"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     misc_feature    5488..5536
                     /label="Synthetic Poly(A) signal"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 GGCCTAACTG GCCTCAATAT TGGCCATTAG CCATATTATT CATTGGTTAT ATAGCATAAA
       61 TCAATATTGG CTATTGGCCA TTGCATACGT TGTATCTATA TCATAATATG TACATTTATA
      121 TTGGCTCATG TCCAATATGA CCGCCATGTT GGCATTGATT ATTGACTAGT TATTAATAGT
      181 AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA
      241 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA
      301 CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT
      361 TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT CCGCCCCCTA
      421 TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTACGGG
      481 ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT
      541 TTTGGCAGTA CACCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC
      601 ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT
      661 GTCGTAATAA CCCCGCCCCG TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
      721 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCACTAG AAGCTTTATT GCGGTAGTTT
      781 ATCACAGTTA AATTGCTAAC GCAGTCAGTG GGCCTCGGCG GCCAAGCTTG GCAATCCGGT
      841 ACTGTTGGTA AAGCCACCAT GGTCTTCACA CTCGAAGATT TCGTTGGGGA CTGGCGACAG
      901 ACAGCCGGCT ACAACCTGGA CCAAGTCCTT GAACAGGGAG GTGTGTCCAG TTTGTTTCAG
      961 AATCTCGGGG TGTCCGTAAC TCCGATCCAA AGGATTGTCC TGAGCGGTGA AAATGGGCTG
     1021 AAGATCGACA TCCATGTCAT CATCCCGTAT GAAGGTCTGA GCGGCGACCA AATGGGCCAG
     1081 ATCGAAAAAA TTTTTAAGGT GGTGTACCCT GTGGATGATC ATCACTTTAA GGTGATCCTG
     1141 CACTATGGCA CACTGGTAAT CGACGGGGTT ACGCCGAACA TGATCGACTA TTTCGGACGG
     1201 CCGTATGAAG GCATCGCCGT GTTCGACGGC AAAAAGATCA CTGTAACAGG GACCCTGTGG
     1261 AACGGCAACA AAATTATCGA CGAGCGCCTG ATCAACCCCG ACGGCTCCCT GCTGTTCCGA
     1321 GTAACCATCA ACGGAGTGAC CGGCTGGCGG CTGTGCGAAC GCATTCTGGC GAATTCTCAC
     1381 GGCTTTCCGC CTGAGGTTGA AGAGCAAGCC GCCGATACAT TGCCTATGTC CTGCGCACAA
     1441 GAAAGCGGTA TGGACCGGCA CCCAGCCGCT TGTGCTTCAG CTCGCATCAA CGTCTAAGGC
     1501 CGCGACTCTA GAGTCGTTCT AGGCGATCGC TCGAGAAAAC ATGAGGATCA CCCATGTCTG
     1561 CAGGTCGACT CTAGAAACGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA
     1621 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC
     1681 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT
     1741 TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA
     1801 ATGTGGTAAA ATCGATAAGG ATCCGTTTGC GTATTGGGCG CTCTTCCGCT GATCTGCGCA
     1861 GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGCTACCTT CTGAGGCGGA
     1921 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
     1981 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
     2041 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
     2101 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
     2161 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA
     2221 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCGATTCT
     2281 TCTGACACTA GCGCCACCAT GAAGAAGCCC GAACTCACCG CTACCAGCGT TGAAAAATTT
     2341 CTCATCGAGA AGTTCGACAG TGTGAGCGAC CTGATGCAGT TGTCGGAGGG CGAAGAGAGC
     2401 CGAGCCTTCA GCTTCGATGT CGGCGGACGC GGCTATGTAC TGCGGGTGAA TAGCTGCGCT
     2461 GATGGCTTCT ACAAAGACCG CTACGTGTAC CGCCACTTCG CCAGCGCTGC ACTACCCATC
     2521 CCCGAAGTGT TGGACATCGG CGAGTTCAGC GAGAGCCTGA CATACTGCAT CAGTAGACGC
     2581 GCCCAAGGCG TTACTCTCCA AGACCTCCCC GAAACAGAGC TGCCTGCTGT GTTACAGCCT
     2641 GTCGCCGAAG CTATGGATGC TATTGCCGCC GCCGACCTCA GTCAAACCAG CGGCTTCGGC
     2701 CCATTCGGGC CCCAAGGCAT CGGCCAGTAC ACAACCTGGC GGGATTTCAT TTGCGCCATT
     2761 GCTGATCCCC ATGTCTACCA CTGGCAGACC GTGATGGACG ACACCGTGTC CGCCAGCGTA
     2821 GCTCAAGCCC TGGACGAACT GATGCTGTGG GCCGAAGACT GTCCCGAGGT GCGCCACCTC
     2881 GTCCATGCCG ACTTCGGCAG CAACAACGTC CTGACCGACA ACGGCCGCAT CACCGCCGTA
     2941 ATCGACTGGT CCGAAGCTAT GTTCGGGGAC AGTCAGTACG AGGTGGCCAA CATCTTCTTC
     3001 TGGCGGCCCT GGCTGGCTTG CATGGAGCAG CAGACTCGCT ACTTCGAGCG CCGGCATCCC
     3061 GAGCTGGCCG GCAGCCCTCG TCTGCGAGCC TACATGCTGC GCATCGGCCT GGATCAGCTC
     3121 TACCAGAGCC TCGTGGACGG CAACTTCGAC GATGCTGCCT GGGCTCAAGG CCGCTGCGAT
     3181 GCCATCGTCC GCAGCGGGGC CGGCACCGTC GGTCGCACAC AAATCGCTCG CCGGAGCGCA
     3241 GCCGTATGGA CCGACGGCTG CGTCGAGGTG CTGGCCGACA GCGGCAACCG CCGGCCCAGT
     3301 ACACGACCGC GCGCTAAGGA GGTAGGTCGA GTTTAAACTC TAGAACCGGT CATGGCCGCA
     3361 ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGTT CGAACTAGAT
     3421 GCTGTCGACC GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG
     3481 GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG
     3541 TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
     3601 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
     3661 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
     3721 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
     3781 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
     3841 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
     3901 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG
     3961 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
     4021 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
     4081 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
     4141 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG
     4201 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
     4261 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
     4321 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     4381 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     4441 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGCGGCCGC
     4501 AAATGCTAAA CCACTGCAGT GGTTACCAGT GCTTGATCAG TGAGGCACCG ATCTCAGCGA
     4561 TCTGCCTATT TCGTTCGTCC ATAGTGGCCT GACTCCCCGT CGTGTAGATC ACTACGATTC
     4621 GTGAGGGCTT ACCATCAGGC CCCAGCGCAG CAATGATGCC GCGAGAGCCG CGTTCACCGG
     4681 CCCCCGATTT GTCAGCAATG AACCAGCCAG CAGGGAGGGC CGAGCGAAGA AGTGGTCCTG
     4741 CTACTTTGTC CGCCTCCATC CAGTCTATGA GCTGCTGTCG TGATGCTAGA GTAAGAAGTT
     4801 CGCCAGTGAG TAGTTTCCGA AGAGTTGTGG CCATTGCTAC TGGCATCGTG GTATCACGCT
     4861 CGTCGTTCGG TATGGCTTCG TTCAACTCTG GTTCCCAGCG GTCAAGCCGG GTCACATGAT
     4921 CACCCATATT ATGAAGAAAT GCAGTCAGCT CCTTAGGGCC TCCGATCGTT GTCAGAAGTA
     4981 AGTTGGCCGC GGTGTTGTCG CTCATGGTAA TGGCAGCACT ACACAATTCT CTTACCGTCA
     5041 TGCCATCCGT AAGATGCTTT TCCGTGACCG GCGAGTACTC AACCAAGTCG TTTTGTGAGT
     5101 AGTGTATACG GCGACCAAGC TGCTCTTGCC CGGCGTCTAT ACGGGACAAC ACCGCGCCAC
     5161 ATAGCAGTAC TTTGAAAGTG CTCATCATCG GGAATCGTTC TTCGGGGCGG AAAGACTCAA
     5221 GGATCTTGCC GCTATTGAGA TCCAGTTCGA TATAGCCCAC TCTTGCACCC AGTTGATCTT
     5281 CAGCATCTTT TACTTTCACC AGCGTTTCGG GGTGTGCAAA AACAGGCAAG CAAAATGCCG
     5341 CAAAGAAGGG AATGAGTGCG ACACGAAAAT GTTGGATGCT CATACTCGTC CTTTTTCAAT
     5401 ATTATTGAAG CATTTATCAG GGTTACTAGT ACGTCTCTCA AGGATAAGTA AGTAATATTA
     5461 AGGTACGGGA GGTATTGGAC AGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT
     5521 GTTGGTTTTT TGTGTGAATC GATAGTACTA ACATACGCTC TCCATCAAAA CAAAACGAAA
     5581 CAAAACAAAC TAGCAAAATA GGCTGTCCCC AGTGCAAGTG CAGGTGCCAG AACATTTCTC
     5641 T
//