LOCUS       pNL3.2.CMV.CDS2-TH.NLuc 5650 bp ds-DNA     circular     10-JUL-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    14..755
                     /label="CMV immediate early enhancer/promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    720..726
                     /label="TATA box"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    734..747
                     /label="crs"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="cis-repression sequence"
     misc_feature    748..748
                     /label=+1
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="transcription start site"
     misc_feature    748..755
                     /label="inr"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="initiator-like sequence"
     misc_feature    853..858
                     /label="Kozac"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    859..1392
                     /label="NanoLuc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             859..1518
                     /label="Translation 859-1518"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWVHEDHPCNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILANSHGFPPEVEEQAADTLPMSCAQESGMDRHPAACASARINV*"
     misc_feature    complement(1239..1260)
                     /label="For invPCR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1261..1270
                     /label="CM4 addition"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1261..1281
                     /label="Target Hairpin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1271..1281
                     /label="CM3 addition"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1282..1305
                     /label="For invPCR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1396..1515
                     /label="PEST sequence from Mm ornithine decarboxylase"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1596..1817
                     /label="SV40 late poly(A) region"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1715..1720
                     /label="A2UA3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1865..2283
                     /label="SV40 early enhancer/promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             2308..3345
                     /label="Translation 2308-3345"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKEVGRV*"
     misc_feature    2308..3345
                     /label="Hygromycin resistance gene"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3369..3417
                     /label="Synthetic Poly-(A) Signal"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3741..3777
                     /label="Origin Element"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4532..5392
                     /label="Beta-lactamse (Amp resistance)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             complement(4532..5392)
                     /label="Translation 5392-4532"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     misc_feature    5497..5545
                     /label="Synthetic Poly(A) signal"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 GGCCTAACTG GCCTCAATAT TGGCCATTAG CCATATTATT CATTGGTTAT ATAGCATAAA
       61 TCAATATTGG CTATTGGCCA TTGCATACGT TGTATCTATA TCATAATATG TACATTTATA
      121 TTGGCTCATG TCCAATATGA CCGCCATGTT GGCATTGATT ATTGACTAGT TATTAATAGT
      181 AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA
      241 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA
      301 CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT
      361 TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT CCGCCCCCTA
      421 TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTACGGG
      481 ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT
      541 TTTGGCAGTA CACCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC
      601 ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT
      661 GTCGTAATAA CCCCGCCCCG TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
      721 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCACTAG AAGCTTTATT GCGGTAGTTT
      781 ATCACAGTTA AATTGCTAAC GCAGTCAGTG GGCCTCGGCG GCCAAGCTTG GCAATCCGGT
      841 ACTGTTGGTA AAGCCACCAT GGTCTTCACA CTCGAAGATT TCGTTGGGGA CTGGCGACAG
      901 ACAGCCGGCT ACAACCTGGA CCAAGTCCTT GAACAGGGAG GTGTGTCCAG TTTGTTTCAG
      961 AATCTCGGGG TGTCCGTAAC TCCGATCCAA AGGATTGTCC TGAGCGGTGA AAATGGGCTG
     1021 AAGATCGACA TCCATGTCAT CATCCCGTAT GAAGGTCTGA GCGGCGACCA AATGGGCCAG
     1081 ATCGAAAAAA TTTTTAAGGT GGTGTACCCT GTGGATGATC ATCACTTTAA GGTGATCCTG
     1141 CACTATGGCA CACTGGTAAT CGACGGGGTT ACGCCGAACA TGATCGACTA TTTCGGACGG
     1201 CCGTATGAAG GCATCGCCGT GTTCGACGGC AAAAAGATCA CTGTAACAGG GACCCTGTGG
     1261 GTACATGAGG ATCACCCATG TAACGGCAAC AAAATTATCG ACGAGCGCCT GATCAACCCC
     1321 GACGGCTCCC TGCTGTTCCG AGTAACCATC AACGGAGTGA CCGGCTGGCG GCTGTGCGAA
     1381 CGCATTCTGG CGAATTCTCA CGGCTTTCCG CCTGAGGTTG AAGAGCAAGC CGCCGATACA
     1441 TTGCCTATGT CCTGCGCACA AGAAAGCGGT ATGGACCGGC ACCCAGCCGC TTGTGCTTCA
     1501 GCTCGCATCA ACGTCTAAGG CCGCGACTCT AGAGTCGCTC GAGGGCTGGT ACCGTTGCTA
     1561 GCACGAGCGG CCGCTTACCG GCCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA
     1621 GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA
     1681 TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG
     1741 CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA
     1801 CCTCTACAAA TGTGGTAAAA TCGATAAGGA TCCGTTTGCG TATTGGGCGC TCTTCCGCTG
     1861 ATCTGCGCAG CACCATGGCC TGAAATAACC TCTGAAAGAG GAACTTGGTT AGCTACCTTC
     1921 TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC
     1981 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA
     2041 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA
     2101 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT
     2161 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC
     2221 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
     2281 CTCGATTCTT CTGACACTAG CGCCACCATG AAGAAGCCCG AACTCACCGC TACCAGCGTT
     2341 GAAAAATTTC TCATCGAGAA GTTCGACAGT GTGAGCGACC TGATGCAGTT GTCGGAGGGC
     2401 GAAGAGAGCC GAGCCTTCAG CTTCGATGTC GGCGGACGCG GCTATGTACT GCGGGTGAAT
     2461 AGCTGCGCTG ATGGCTTCTA CAAAGACCGC TACGTGTACC GCCACTTCGC CAGCGCTGCA
     2521 CTACCCATCC CCGAAGTGTT GGACATCGGC GAGTTCAGCG AGAGCCTGAC ATACTGCATC
     2581 AGTAGACGCG CCCAAGGCGT TACTCTCCAA GACCTCCCCG AAACAGAGCT GCCTGCTGTG
     2641 TTACAGCCTG TCGCCGAAGC TATGGATGCT ATTGCCGCCG CCGACCTCAG TCAAACCAGC
     2701 GGCTTCGGCC CATTCGGGCC CCAAGGCATC GGCCAGTACA CAACCTGGCG GGATTTCATT
     2761 TGCGCCATTG CTGATCCCCA TGTCTACCAC TGGCAGACCG TGATGGACGA CACCGTGTCC
     2821 GCCAGCGTAG CTCAAGCCCT GGACGAACTG ATGCTGTGGG CCGAAGACTG TCCCGAGGTG
     2881 CGCCACCTCG TCCATGCCGA CTTCGGCAGC AACAACGTCC TGACCGACAA CGGCCGCATC
     2941 ACCGCCGTAA TCGACTGGTC CGAAGCTATG TTCGGGGACA GTCAGTACGA GGTGGCCAAC
     3001 ATCTTCTTCT GGCGGCCCTG GCTGGCTTGC ATGGAGCAGC AGACTCGCTA CTTCGAGCGC
     3061 CGGCATCCCG AGCTGGCCGG CAGCCCTCGT CTGCGAGCCT ACATGCTGCG CATCGGCCTG
     3121 GATCAGCTCT ACCAGAGCCT CGTGGACGGC AACTTCGACG ATGCTGCCTG GGCTCAAGGC
     3181 CGCTGCGATG CCATCGTCCG CAGCGGGGCC GGCACCGTCG GTCGCACACA AATCGCTCGC
     3241 CGGAGCGCAG CCGTATGGAC CGACGGCTGC GTCGAGGTGC TGGCCGACAG CGGCAACCGC
     3301 CGGCCCAGTA CACGACCGCG CGCTAAGGAG GTAGGTCGAG TTTAAACTCT AGAACCGGTC
     3361 ATGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGTTC
     3421 GAACTAGATG CTGTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT
     3481 GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG
     3541 TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
     3601 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
     3661 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
     3721 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
     3781 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
     3841 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
     3901 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
     3961 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
     4021 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
     4081 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
     4141 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
     4201 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
     4261 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
     4321 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     4381 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     4441 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     4501 AGCGGCCGCA AATGCTAAAC CACTGCAGTG GTTACCAGTG CTTGATCAGT GAGGCACCGA
     4561 TCTCAGCGAT CTGCCTATTT CGTTCGTCCA TAGTGGCCTG ACTCCCCGTC GTGTAGATCA
     4621 CTACGATTCG TGAGGGCTTA CCATCAGGCC CCAGCGCAGC AATGATGCCG CGAGAGCCGC
     4681 GTTCACCGGC CCCCGATTTG TCAGCAATGA ACCAGCCAGC AGGGAGGGCC GAGCGAAGAA
     4741 GTGGTCCTGC TACTTTGTCC GCCTCCATCC AGTCTATGAG CTGCTGTCGT GATGCTAGAG
     4801 TAAGAAGTTC GCCAGTGAGT AGTTTCCGAA GAGTTGTGGC CATTGCTACT GGCATCGTGG
     4861 TATCACGCTC GTCGTTCGGT ATGGCTTCGT TCAACTCTGG TTCCCAGCGG TCAAGCCGGG
     4921 TCACATGATC ACCCATATTA TGAAGAAATG CAGTCAGCTC CTTAGGGCCT CCGATCGTTG
     4981 TCAGAAGTAA GTTGGCCGCG GTGTTGTCGC TCATGGTAAT GGCAGCACTA CACAATTCTC
     5041 TTACCGTCAT GCCATCCGTA AGATGCTTTT CCGTGACCGG CGAGTACTCA ACCAAGTCGT
     5101 TTTGTGAGTA GTGTATACGG CGACCAAGCT GCTCTTGCCC GGCGTCTATA CGGGACAACA
     5161 CCGCGCCACA TAGCAGTACT TTGAAAGTGC TCATCATCGG GAATCGTTCT TCGGGGCGGA
     5221 AAGACTCAAG GATCTTGCCG CTATTGAGAT CCAGTTCGAT ATAGCCCACT CTTGCACCCA
     5281 GTTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCGGG GTGTGCAAAA ACAGGCAAGC
     5341 AAAATGCCGC AAAGAAGGGA ATGAGTGCGA CACGAAAATG TTGGATGCTC ATACTCGTCC
     5401 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTACTAGTA CGTCTCTCAA GGATAAGTAA
     5461 GTAATATTAA GGTACGGGAG GTATTGGACA GGCCGCAATA AAATATCTTT ATTTTCATTA
     5521 CATCTGTGTG TTGGTTTTTT GTGTGAATCG ATAGTACTAA CATACGCTCT CCATCAAAAC
     5581 AAAACGAAAC AAAACAAACT AGCAAAATAG GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA
     5641 ACATTTCTCT
//