LOCUS Exported 9954 bp ds-DNA circular SYN 24-JUN-2015 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pROSA26-PA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9954) AUTHORS Addgene TITLE Direct Submission JOURNAL Exported Wednesday, Jun 24, 2015 from SnapGene 2.7.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9954 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 107..128 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 181..197 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" promoter 242..260 /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" polyA_signal 322..546 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" intron 620..685 /note="small t intron" /note="simian virus 40 (SV40) small t antigen intron" promoter complement(1361..1860) /note="PGK promoter" /note="mouse phosphoglycerate kinase 1 promoter" promoter complement(7382..7400) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7410..7426) /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(7567..8022) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 8049..8153 /gene="bla" /note="AmpR promoter" CDS 8154..9014 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 9185..9773 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg 241 caattaaccc tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gagccccagc 301 tggttctttc cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt 361 cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc 421 tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac 481 cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag 541 gcacagtcga ggctgatcag cgagctctag gatctgcatt ccaccactgc tcccattcat 601 cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt 661 atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca 721 attatgtcac accacagaag taaggttcct tcacaaagag atcgcctgac acgatttcct 781 gcacaggctt gagccatata ctcatacatc gcatcttggc cacgttttcc acgggtttca 841 aaattaatct caagttctac gcttaacgct ttcgcctgtt cccagttatt aatatattca 901 acgctagaac tcccctcagc gaagggaagg ctgagcacta cacgcgaagc accatcaccg 961 aaccttttga taaactcttc cgttccgact tgctccatca acggttcagt gagacttaaa 1021 cctaactctt tcttaatagt ttcggcatta tccactttta gtgcgagaac cttcgtcagt 1081 cctggatacg tcactttgac cacgcctcca gcttttccag agagcgggtt ttcattatct 1141 acagagtatc ccgcagcgtc gtatttattg tcggtactat aaaacccttt ccaatcatcg 1201 tcataatttc cttgtgtacc agattttggc ttttgtatac ctttttgaat ggaatctaca 1261 taaccaggtt tagtcccgtg gtacgaagaa aagttttcca tcacaaaaga tttagaagaa 1321 tcaacaacat catcaggatc catggcgagg acctgcaggt cgaaaggccc ggagatgagg 1381 aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg 1441 aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac 1501 cccttcccag cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc 1561 aaaggcctac ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac 1621 gtgctacttc catttgtcac gtcctgcacg acgcgagctg cggggcgggg gggaacttcc 1681 tgactagggg aggagtagaa ggtggcgcga aggggccacc aaagaacgga gccggttggc 1741 gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc 1801 ccagcggggc tgctaaagcg catgctccag actgccttgg gaaaagcgcc tcccctaccc 1861 ggtagaattc gatatcaagc ttatcgatac cgtcgacggt atcgataagc tagatgtcct 1921 gaaatatttc tatcagaaca aggtagtata aagctggtag gtatacaaaa cgctagacta 1981 gtttctatcc ctgaccctta atctgctagt atatccgtag gaagttgctt aagtgccact 2041 agtaccaaca gcctctgtta gagtgaacag tagattttaa tgaagggcca ataaagacca 2101 cttaagacta ctgactgaat taaaaatcca cccagtggag ttagtattgg ttatagttat 2161 ataacaaaat atccaactta gccaggcgtg gtggtgcacg cctttaatcc cggcactcag 2221 gaggcagagg caggcggatt tctgagttcg aggccagcct ggtctacaaa gtgagttcca 2281 ggacagccag ggctatacag agaaaccctg tctcgaaaaa ccaaaaaaaa aaaaaaaaag 2341 gaaaaaaaat atggcttgaa aacatgctat tgttgtcaaa aggagatcca aacaagtatt 2401 tctttgaaca aaaattatgt agaaaacagg gtgataaaga acaagggctt tttaaaaaag 2461 gccagcatta tttgagatat acaagtgact ccaattcaag cctaaaggcc actcaatgct 2521 cactaacagt gctaatcaga atcaattcct tagcccagtc aaaaatagtc ctgaatatct 2581 tctggataaa catgtcctcc aacatataaa ctagtataca ctaacaaaac gtctcaactt 2641 caaggtgaaa tgcttgactc ctagacttgt gacccagcaa agtgctctat aggtagggtt 2701 actaggtcag agagtcttgc ctgcaaacca caattataca attccaccaa atgtaagaca 2761 tgtaaaaatg acttatttag ctcaggtttt gaaaattaaa aatcaaaccc acaaagacct 2821 aactttcaca ttaagtacaa atgtttaaca tatataacat gttttgcctt gatatatgaa 2881 atcatatgac atcacctgac aggaaactta agtttatttg aacaacaatc agcctaaggt 2941 aggcctagca catgatcagg atagtgcagg gaaacccaaa gaagtgcttc tgagtataaa 3001 ccaagagacc tcgaaatagc agctttgttc tgtatctcat gggctttaac tatcacttga 3061 aaacaatttc acaaatcaca aaaccaacat ttgtttagtt tccaactctg cctattcacc 3121 ggagaaatcc atgtcccaat ttgatggggg aaaacttgaa tgaaatatta tttggaatat 3181 tttaatatcc ctgtgtatat gcagatggtt taaagacagg taaccttaac tgtagtttct 3241 tggttgtctg ccttgttttt tgaaatatgc tctcaccagg agcctgccaa gtaactactc 3301 ttgtgtgctc agcaagtcct agggatccct taagcatgct ctaacaggcc tggctttttt 3361 ttttaaaaag taggttgcgg tgataataat tcaagttttc atcacttgaa acacatttta 3421 ccaactatca cccaagctcc cttagtgtgt cccctataaa agaatctgac ctgcaagttc 3481 caaaagtatt actacatttt aaccttagta ttcaggagac aaaagcaggc agaactttag 3541 tctacatatc ttgttgcaga tcaggcagag atacatagtg agaccctgcc tcaggaaaag 3601 ctatcatttt tatattcaca gtaagcaaat taacattaaa agtcagaaat tcttaatttg 3661 actttggctg tgaagaattt ggattctctg gatttgaaac tagaaaatct gattaagcga 3721 tgcaacagtt tctgtatact aaaactctaa gacccttggt tctaaagata ccacatttaa 3781 aaaaaaagtt aagtataaga caaaaagcct taatagctct tttatagggg agggactcat 3841 ttaatattag tccacctcac tcctcataac tattttatga ggtttattat ataacccatt 3901 ttttaaaaaa aaaattaaag gacaatttta gtgtttgaaa gatttcccaa ccccaccctg 3961 gaaatcaggc tgcaaatctc agcagctgcc cttagaaggc aaaaagccat gccatttaag 4021 ccatgggaag ttagtagcaa acaagagacc acatggcagg aaggaggctt taaagaaagc 4081 ccacagtgtc aaaagaccca accaacagca gagacagaaa aaataatacc caaatctcat 4141 cagaaaggta gacggattta gccacatcca tagtggctca ttagggaatg cttcaattat 4201 aattttatga aatctcagag cccccctccc caacagggtt tctgtgtgta ttcctggcta 4261 tcctagatag aacttgatgt gtagaccagg ctgggctcaa actcagagat ctgcctgtct 4321 ctgcctccag agtgctggga ttaaaggcat aaaggcaagc accaccactg gctggctaaa 4381 ctctggccct acatttttga aatccaaaat tatcattact tgctttcatg tttagtaatg 4441 gctgcagact tagctttcag ctttgtatag atgaagcaca aaagttaagt aaagctagca 4501 gtttaaacaa ataggatact agaagttcaa tttttatttc tttattgctt agtgtaagag 4561 aaaagacatt aaagtctgca ttggtcttct gtatcccaca agtctgcagt tatggctcct 4621 ctgtccacag ttacacttca aataaaaatt agttccttgg taacatttcc agtgcttaaa 4681 gagtaatttg attattggct accatattgg aacaaacaca aagtatttca ttacttcagt 4741 aaattctact ggagacagta ttctctttaa aaaaaaaaaa aagaagaaga aggaggattt 4801 atttatttaa tgtgaataca cttgtggtct tcagacacac cagaagaggg catcagatcc 4861 cattacagat ggttgtgagc caccatgtgg ttgctgggaa ttgaactcag gacttctgga 4921 agagcagtct gtgctcttga actccgagcc acctctccag cccctggata gtattattat 4981 agagcacaag cacacacaac acaacaacct aaaaaccaag cttcacaaat acatttgttg 5041 cttaatcatt ttacttgtgt ttggctacaa gtcaagcaaa attataggtc ctgaagaagc 5101 ttggcaaaat cacatttaga ccagcaataa cgtgtagaat gccatgagtc aagccagtcc 5161 aagagaaagc actgcttaaa tagaaaaaat acaagctcac aagaccttag gtcaggaaag 5221 acaacaaaca cctgaacttt gcattccaaa aggaaccacc ttttacagat gtgtacaatg 5281 acaaaatttt agtaagcagt aatcaatacc atgtggctca ataatgaaat atacctttta 5341 atgtcttaaa atatcagcct ggcaatatgt aagatacatc aggtaatatt gggggaggag 5401 acatccacct ggaaaccatt aatggttaat agctcagttt ataaatggag aaaaaggaga 5461 gaggcattca tgggagtgga aagttaagct ttaggttgga ttctcaatac atctattgtt 5521 gtaaaactcc ctggactgag aataggccca aatgtggaac accacctgac gggagaggtg 5581 atagacactg aaaattaagg atcaaggcaa aggatcaaca aaaagtgtac taaggagtta 5641 taaaagaact gcagtgttga ggccccagct acagcctcga tttgtggtgt atgtaactaa 5701 tctgtctggt ttcatgagtc atcagacttc taagatcagg aaagggaaaa tgccaatgct 5761 ctgtctaggg gttggataag ccagtataat aaatgaaaat ggggctaaaa tgagtgttct 5821 aaaatacctt ttgataaggc tgcagaagga gcgggagaaa tggatatgaa gtactgggct 5881 ctttaaaaat gattaaaatt ctgcttacat agtctaactc gcgacactgt aatttcatac 5941 tgtagtaagg atctcaagca ggagagtata aaactcgggt gagcatgtct ttaatctacc 6001 tcgatggaaa atactccgag gcggatcaca agcaataata acctgtagtt ttgctgcata 6061 aaaccccaga tgactaccta tcctcccatt ttccttattt gcccctatta aaaaacttcc 6121 cgacaaaacc gaaaatctgt gggaagtctt gtccctccaa ttttacacct gttcaattcc 6181 cctgcaggac aacgcccaca caccaggtta gcctttaagc ctgcccagaa gactcccgcc 6241 catcttctag ccggcgcgcc atttaaatta attaaggcta gaaagactgg agttgcagat 6301 cacgagggaa gagggggaag ggattctccc aggcccaggg cggtcctcag aagccaggag 6361 gcagcagaga actcccagaa aggtattgca acactcccct cccccctccg gagaagggtg 6421 cggccttctc cccgcctact ccactgcagc tcccttactg ataacaactc agagcgactt 6481 tgggagagca agtgcttcct gcctccaaaa cagcccaact gagccctcgc tccttccctc 6541 cactccccgg agtgcgcgat ggaggtctgg ctcagcacgc ccctcttgag gcaactcaag 6601 tcggaaacgt gcttgcaccc gccccgcagc cgctcagccc tactgcccgt ccccgccccc 6661 agcgcgcgct tcctgccacg ttgcgcaggg gcgcgcggcc agactctgcg gcgcggggcc 6721 gaggggaggg ccggaacctg ggagcgcctc ctcgccgccc ccgctggccg gcggatggac 6781 tcaacttgca cgaacacgag ccaatggcaa gggccagttt tctgggcccc gagagccaat 6841 cagacgacga ggcccggccg gcggcgggta aaacgactcc cccagaggaa ggggagggtg 6901 ggcggccgct cgcgcgcgag ctactttcgc tgaccctccc tcccctcccc cgccccgcca 6961 gaggccgacc gcgcccgcac gtccagctcg cctcacccca cctacctccc gccccaccca 7021 gtgggcagag cgaggctgcc ggcggctgcg cactccggct gccgttaact gacaggcgcc 7081 ttacgccaac caaaacacgc catttgtgtt ttcacacacg gcgggaggaa aagaagccaa 7141 tcagcgacga gacgtcggcc ggaagcgctc ctccgctgcc cccccccccc ccccgagcca 7201 tggccgcgtc cggtggagac ttttccgctc ccttctccct ccccctccgg ttgctgcagg 7261 gcggaccgca ttcctgccca ccacccgctt gccccttcca gcgtcacgac tcgtacccgg 7321 ctgtctcaca gaacggctcc accacgctcg gagggcctgc cgcggtggag ctccaattcg 7381 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg 7441 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 7501 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 7561 gaatggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 7621 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 7681 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 7741 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 7801 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 7861 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 7921 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 7981 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg 8041 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 8101 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 8161 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 8221 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 8281 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 8341 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 8401 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 8461 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 8521 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 8581 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 8641 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 8701 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 8761 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 8821 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 8881 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 8941 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 9001 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 9061 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 9121 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 9181 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 9241 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 9301 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 9361 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 9421 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 9481 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 9541 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 9601 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 9661 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 9721 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 9781 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 9841 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 9901 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag //