LOCUS       Exported                8706 bp DNA     circular SYN 10-OCT-2023
DEFINITION  This is based on the Alexander Deiters-lab plasmid 
            U6H1-mCherry-TAG-EGFP-PylT.ape, later at the MH-lab renamed pAD004.
            
            Ampicillin resistance in E coli, Neomycin resistance in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 10, 2023 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="pcDNA3"
                     /mol_type="other DNA"
                     /label=source:pcDNA3
                     /note="/locus_tag=source:pcDNA3"
                     /note="/vntifkey=98"
                     /note="color: #ffc0cb"
     misc_feature    complement(30..98)
                     /locus_tag="Mb\pylT"
                     /label=Mb\pylT
                     /note="/vntifkey=21"
                     /note="color: #80ff80; direction: LEFT"
     misc_feature    complement(99..324)
                     /locus_tag="H1"
                     /label=H1
                     /note="/vntifkey=21"
                     /note="color: #80ff80; direction: LEFT"
     misc_feature    complement(360..428)
                     /locus_tag="Dh\pylT"
                     /label=Dh\pylT
                     /note="/vntifkey=21"
                     /note="color: #80ff80; direction: LEFT"
     misc_feature    complement(429..685)
                     /locus_tag="U6"
                     /label=U6
                     /note="/vntifkey=21"
                     /note="color: #80ff80; direction: LEFT"
     promoter        761..1377
                     /locus_tag="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
                     /note="/vntifkey=30"
                     /note="color: #ffffff"
     misc_feature    840..1127
                     /locus_tag="CAG_enhancer"
                     /label=CAG_enhancer
                     /note="/vntifkey=21"
                     /note="color: #ffffff"
     promoter        1388..1406
                     /locus_tag="T7_promoter"
                     /label=T7_promoter
                     /note="/vntifkey=30"
                     /note="color: #346ee0"
     regulatory      1442..1451
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             1448..1498
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /note="color: #993366"
                     /translation="MGVADLIKKFESISKEE"
     CDS             1520..2227
                     /codon_start=1
                     /label=mNeonGreen
                     /note="color: #00ff00"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     misc_feature    2228..2230
                     /label=STOP 1
                     /note="color: #ff0000"
     misc_feature    2231..2233
                     /label=STOP 2
                     /note="color: #ff0000"
     misc_feature    2234..2236
                     /label=STOP 3
                     /note="color: #ff0000"
     misc_feature    2248..2833
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 2248 .. 2821 / #a6acb3
                        2: 2822 .. 2824 / #a6acb3 / ATG
                        3: 2825 .. 2833 / #a6acb3"
     CDS             2834..4132
                     /codon_start=1
                     /label=Talin1 Head (1-433)
                     /note="color: #00ccff"
                     /translation="MVALSLKISIGNVVKTMQFEPSTMVYDACRMIRERIPEALAGPPN
                     DFGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTMEYRKKQRPLKIRMLDGTVKTIMVDD
                     SKTVTDMLMTICARIGITNHDEYSLVRELMEEKKDEGTGTLRKDKTLLRDEKKMEKLKQ
                     KLHTDDELNWLDHGRTLREQGVEEHETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDD
                     ILNGSHPVSFDKACEFAGFQCQIQFGPHNEQKHKAGFLDLKDFLPKEYVKQKGERKIFQ
                     AHKNCGQMSEIEAKVRYVKLARSLKTYGVSFFLVKEKMKGKNKLVPRLLGITKECVMRV
                     DEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQIAQLIAGYIDII
                     LKKKKSKDHFGLEGDEESTMLEDSVSPKKSTVLQ"
     CDS             4133..4141
                     /codon_start=1
                     /label=GSG linker
                     /note="color: #c0c0c0"
                     /translation="GSG"
     CDS             4142..4189
                     /codon_start=1
                     /label=SpyTag003
                     /note="color: #ff6600"
                     /translation="RGVPHIVMVDAYKRYK"
     misc_feature    4190..4192
                     /label=STOP 1
                     /note="color: #ff0000"
     misc_feature    4193..4195
                     /label=STOP 2
                     /note="color: #ff0000"
     misc_feature    4196..4198
                     /label=STOP 3
                     /note="color: #ff0000"
     misc_feature    complement(4260..4277)
                     /locus_tag="Sp6_primer"
                     /label=Sp6_primer
                     /note="/vntifkey=21"
                     /note="color: #80ff80; direction: LEFT"
     promoter        complement(4261..4278)
                     /locus_tag="Sp6_promoter"
                     /label=Sp6_promoter
                     /note="/vntifkey=30"
                     /note="color: #346ee0; direction: LEFT"
     misc_feature    complement(4297..4314)
                     /locus_tag="BGH_rev_primer"
                     /label=BGH_rev_primer
                     /note="/vntifkey=21"
                     /note="color: #80ff80; direction: LEFT"
     terminator      4300..4527
                     /locus_tag="bGH_PA_terminator"
                     /label=bGH_PA_terminator
                     /note="/vntifkey=43"
                     /note="color: #9d1b1c"
     rep_origin      4590..4896
                     /locus_tag="f1_origin"
                     /label=f1_origin
                     /note="/vntifkey=33"
                     /note="color: #999999"
     misc_feature    complement(5010..5030)
                     /locus_tag="pBABE_3_primer"
                     /label=pBABE_3_primer
                     /note="/vntifkey=21"
                     /note="color: #80ff80; direction: LEFT"
     misc_feature    complement(5016..5231)
                     /locus_tag="SV40_enhancer"
                     /label=SV40_enhancer
                     /note="/vntifkey=21"
                     /note="color: #80ff80; direction: LEFT"
     promoter        5028..5296
                     /locus_tag="SV40_promoter"
                     /label=SV40_promoter
                     /note="/vntifkey=30"
                     /note="color: #346ee0"
     rep_origin      5195..5272
                     /locus_tag="SV40_origin"
                     /label=SV40_origin
                     /note="/vntifkey=33"
                     /note="color: #999999"
     misc_feature    5257..5276
                     /locus_tag="SV40pro_F_primer"
                     /label=SV40pro_F_primer
                     /note="/vntifkey=21"
                     /note="color: #80ff80"
     CDS             5411..6205
                     /gene="NeoR/KanR"
                     /locus_tag="NeoR/KanR"
                     /label=NeoR/KanR
                     /note="ORF frame 3"
                     /note="/vntifkey=4"
                     /note="color: #e9d024; direction: RIGHT"
     gene            5414..6202
                     /gene="NeoR/KanR"
                     /locus_tag="NeoR/KanR(1)"
                     /label=NeoR/KanR
                     /label=NeoR/KanR(1)
                     /note="/vntifkey=60"
                     /note="color: #ff797d; direction: RIGHT"
     terminator      6382..6501
                     /locus_tag="SV40_PA_terminator"
                     /label=SV40_PA_terminator
                     /note="/vntifkey=43"
                     /note="color: #9d1b1c"
     misc_feature    6470..6489
                     /locus_tag="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /note="/vntifkey=21"
                     /note="color: #80ff80"
     misc_feature    complement(6545..6563)
                     /locus_tag="M13_reverse_primer"
                     /label=M13_reverse_primer
                     /note="/vntifkey=21"
                     /note="color: #80ff80; direction: LEFT"
     misc_feature    complement(6562..6584)
                     /locus_tag="M13_pUC_rev_primer"
                     /label=M13_pUC_rev_primer
                     /note="/vntifkey=21"
                     /note="color: #80ff80; direction: LEFT"
     promoter        complement(6598..6627)
                     /locus_tag="lac_promoter"
                     /label=lac_promoter
                     /note="/vntifkey=30"
                     /note="color: #346ee0; direction: LEFT"
     rep_origin      complement(6936..7555)
                     /direction=LEFT
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="/vntifkey=33"
                     /note="color: #999999"
     CDS             complement(7710..8570)
                     /gene="Ampicillin"
                     /locus_tag="Ampicillin"
                     /label=Ampicillin
                     /note="ORF frame 1"
                     /note="/vntifkey=4"
                     /note="color: #e9d024; direction: LEFT"
     gene            complement(7710..8570)
                     /gene="Ampicillin"
                     /locus_tag="Ampicillin(1)"
                     /label=Ampicillin
                     /label=Ampicillin(1)
                     /note="/vntifkey=60"
                     /note="color: #ff102f; direction: LEFT"
     promoter        complement(8612..8640)
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/vntifkey=30"
                     /note="color: #346ee0; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctaga tccaaaaaac ggaaaccccg ggaatctaac ccggctgaac
       61 ggatttagag tccgttcgat ctacatgatc aggttcccag agctgtggtc tcatacagaa
      121 cttataagat tcccaaatcc aaagacattt cacgtttatg gtgatttccc agaacacata
      181 gcgacatgca aatattgcag ggcgccactc ccctgtccct cacagccatc ttcctgccag
      241 ggcgcacgcg cgctgggtgt tcccgcctag tgacactggg cccgcgattc cttggagcgg
      301 gttgatgacg tcagcgttcg aattgactag caaaaaccgc acttgtcaga tccaaaaaac
      361 ggggagtacg ggagtttcac ccgcctgcac gaatttagag tccgtgtgat ctattcgatc
      421 aacccccccg tcctttccac aagatatata aagccaagaa atcgaaatac tttcaagtta
      481 cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac tacccaagaa
      541 attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca gtcaaattaa
      601 ttctaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga atcatgggaa
      661 ataggccctc ttcctgcccg accttcaatt gcatgaagaa tctgcttagg gttaggcgtt
      721 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      781 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      841 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      901 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      961 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     1021 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     1081 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     1141 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     1201 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1261 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     1321 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     1381 tcgaaattaa tacgactcac tatagggaga cccaagcttg gtaccgagct cggatcccgc
     1441 tgccaccatg ggcgtggccg acctgatcaa gaagttcgag agcatcagca aggaggaggg
     1501 ggatccaccg gtcgcaacta tggtgtccaa gggcgaagag gacaacatgg ccagcctgcc
     1561 tgctacacac gagctgcaca tcttcggcag catcaacggc gtggacttcg acatggtcgg
     1621 acaaggcaca ggcaacccca acgacggata cgaggaactg aacctgaagt ccacaaaggg
     1681 cgacctgcag ttcagcccct ggatcctggt gcctcacatc ggctacggct tccaccagta
     1741 cctgccttat cctgacggca tgagcccttt ccaggccgct atggtggatg gctctggata
     1801 ccaggtgcac agaaccatgc agttcgagga cggcgccagc ctgaccgtga actacagata
     1861 cacatacgag ggcagccaca tcaaaggcga ggcccaagtg aaaggcaccg gctttccagc
     1921 tgatggcccc gtgatgacca actctctgac agccgccgac tggtgcagaa gcaagaaaac
     1981 ataccctaac gacaagacca tcatcagcac cttcaagtgg tcctacacca ccggcaacgg
     2041 caagagatac agaagcaccg ccagaaccac ctacaccttc gccaagccta tggccgccaa
     2101 ctacctgaag aaccagccta tgtacgtgtt ccgcaagacc gagctgaagc actccaagac
     2161 agaactgaac ttcaaagagt ggcagaaagc cttcaccgac gtgatgggca tggacgagct
     2221 gtacaagtaa taatgacccg ggatccgccc ctctccctcc ccccccccta acgttactgg
     2281 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     2341 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     2401 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     2461 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     2521 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     2581 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     2641 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     2701 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     2761 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     2821 tatggccaca accatggttg cgctttcgct gaagattagc attgggaatg tggtgaagac
     2881 gatgcaattt gagccatcta ccatggtgta cgacgcctgc cgcatgattc gtgagcggat
     2941 cccagaggcc ctggctggcc ctcccaacga ctttgggctc tttctgtcag atgatgaccc
     3001 caaaaaaggc atctggctag aggctgggaa agctttggac tactacatgc tccgaaatgg
     3061 ggacaccatg gagtacagaa aaaagcagag acccctgaag atccggatgt tagacggaac
     3121 ggtgaagact atcatggtgg atgactccaa gaccgtcaca gacatgctca tgaccatttg
     3181 tgcccgaatt ggtatcacca accatgatga gtattcactg gttcgagagc tgatggaaga
     3241 aaagaaagat gaggggacag ggaccctgag aaaagacaag accctactgc gagatgaaaa
     3301 gaagatggag aagctaaagc agaagttgca cacagacgat gagttgaact ggctggacca
     3361 tgggcggaca ctgagggaac agggagtgga agagcatgag acgttgctgc tgcggagaaa
     3421 gttcttctac tcagaccaga atgtggattc ccgagaccct gtacagctga accttctcta
     3481 tgtgcaggca cgagatgaca tcctgaatgg ctcccatcct gtctcctttg acaaggcctg
     3541 tgaatttgca ggcttccagt gccagatcca gtttggacct cacaatgaac agaagcacaa
     3601 ggctggcttc cttgacctga aggacttcct gcccaaggag tatgtgaagc agaagggaga
     3661 gcgtaagatc tttcaggcac acaagaattg tgggcagatg agtgaaattg aggccaaggt
     3721 acgctacgtg aagctggccc gttccctcaa gacttacggt gtctccttct tcctagtcaa
     3781 ggaaaagatg aaggggaaga ataaactggt gcccaggctg ttgggcatca ctaaggagtg
     3841 tgtgatgcgt gtggatgaga agaccaagga ggtgattcag gaatggagcc tcaccaacat
     3901 caaacgctgg gctgcctctc ccaagagctt tactctggac tttggagact atcaggatgg
     3961 ctactactca gtacagacga cagaaggcga gcagatcgca cagctcattg ctggctacat
     4021 agatatcatc cttaagaaga aaaaaagcaa ggaccatttt gggctggagg gagatgaaga
     4081 gtctactatg ttggaggact cagtgtcacc caaaaagtca acagtccttc agggaagcgg
     4141 ccgtggcgtg cctcatatcg tgatggtgga cgcctacaag cgttacaagt aatgataaga
     4201 attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag ggccctattc
     4261 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     4321 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4381 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4441 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4501 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4561 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4621 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4681 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4741 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4801 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4861 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4921 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4981 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     5041 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     5101 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5161 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5221 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5281 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5341 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     5401 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5461 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5521 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5581 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5641 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5701 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5761 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5821 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5881 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5941 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6001 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6061 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6121 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6181 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6241 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6301 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6361 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6421 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6481 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6541 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6601 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6661 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6721 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6781 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6841 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7141 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7381 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7681 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7741 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7801 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7861 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7921 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7981 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8041 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8101 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8161 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8221 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8281 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8341 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8401 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8461 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8521 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8581 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8641 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8701 gacgtc
//
