LOCUS       Exported               11053 bp DNA     circular SYN 22-DEC-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Protein Dynamics
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 22, 2023 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11053
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     518..535
                     /label=CMV
                     /note="color: black; sequence: acgcaaatgggcggtagg"
     misc_feature    612..617
                     /label=Met-Gly before SpyTag003
                     /note="color: #00ff00"
     misc_feature    618..665
                     /label=SpyTag003
                     /note="color: #ffff00"
     misc_feature    636..638
                     /label=V114T Mutation
                     /note="color: #ff9900"
     misc_feature    642..644
                     /label=V116T Mutation
                     /note="color: #ff9900"
     CDS             666..6989
                     /codon_start=1
                     /label=Talin1 rod domain (434-2541)
                     /note="color: #993366"
                     /translation="QQYNRVGKVEHGSVALPAIMRSGASGPENFQVGSMPPAQQQITSG
                     QMHRGHMPPLTSAQQALTGTINSSMQAVQAAQATLDDFETLPPLGQDAASKAWRKNKMD
                     ESKHEIHSQVDAITAGTASVVNLTAGDPAETDYTAVGCAVTTISSNLTEMSRGVKLLAA
                     LLEDEGGNGRPLLQAAKGLAGAVSELLRSAQPASAEPRQNLLQAAGNVGQASGELLQQI
                     GESDTDPHFQDVLMQLANAVASAAAALVLKAKSVAQRTEDSGLQTQVIAAATQCALSTS
                     QLVACTKVVAPTISSPVCQEQLVEAGRLVAKAVEGCVSASQAATEDGQLLRGVGAAATA
                     VTQALNELLQHVKAHATGAGPAGRYDQATDTILTVTENIFSSMGDAGEMVRQARILAQA
                     TSDLVNAIKADAEGESDLENSRKLLSAAKILADATAKMVEAAKGAAAHPDSEEQQQRLR
                     EAAEGLRMATNAAAQNAIKKKLVQRLEHAAKQAAASATQTIAAAQHAASAPKASAGPQP
                     LLVQSCKAVAEQIPLLVQGVRGSQAQPDSPSAQLALIAASQSFLQPGGKMVAAAKASVP
                     TIQDQASAMQLSQCAKNLGTALAELRTAAQKAQEACGPLEMDSALSVVQNLEKDLQEIK
                     AAARDGKLKPLPGETMEKCTQDLGNSTKAVSSAIAKLLGEIAQGNENYAGIAARDVAGG
                     LRSLAQAARGVAALTSDPAVQAIVLDTASDVLDKASSLIEEAKKASGHPGDPESQQRLA
                     QVAKAVTQALNRCVSCLPGQRDVDNALRAVGDASKRLLSDLLPPSTGTFQEAQSRLNEA
                     AAGLNQAATELVQASRGTPQDLARASGRFGQDFSTFLEAGVEMAGQAPSQEDRAQVVSN
                     LKGISMSSSKLLLAAKALSTDPASPNLKSQLAAAARAVTDSINQLITMCTQQAPGQKEC
                     DNALRQLETVRELLENPVQPINDMSYFGCLDSVMENSKVLGEAMTGISQNAKNGNLPEF
                     GDAIATASKALCGFTEAAAQAAYLVGVSDPNSQAGQQGLVEPTQFARANQAIQMACQSL
                     GEPGCTQAQVLSAATIVAKHTSALCNSCRLASARTANPTAKRQFVQSAKEVANSTANLV
                     KTIKALDGDFTEENRAQCRAATAPLLEAVDNLSAFASNPEFSSVPAQISPEGRAAMEPI
                     VISAKTMLESAGGLIQTARALAVNPRDPPRWSVLAGHSRTVSDSIKKLITSMRDKAPGQ
                     LECETAIAALNSCLRDLDQASLAAVSQQLAPREGISQEALHTQMLTAVQEISHLIEPLA
                     SAARAEASQLGHKVSQMAQYFEPLTLAAVGAASKTLSHPQQMALLDQTKTLAESALQLL
                     YTAKEAGGNPKQAAHTQEALEEAVQMMTEAVEDLTTTLNEAASAAGVVGGMVDSITQAI
                     NQLDEGPMGDPEGSFVDYQTTMVRTAKAIAVTVQEMVTKSNTSPEELGPLANQLTSDYG
                     RLASQAKPAAVAAENEEIGAHIKHRVQELGHGCSALVTKAGALQCSPSDVYTKKELIEC
                     ARRVSEKVSHVLAALQAGNRGTQACITAASAVSGIIADLDTTIMFATAGTLNREGAETF
                     ADHREGILKTAKVLVEDTKVLVQNAAGSQEKLAQAAQSSVATITRLADVVKLGAASLGA
                     EDPETQVVLINAVKDVAKALGDLISATKAAAGKVGDDPAVWQLKNSAKVMVTNVTSLLK
                     TVKAVEDEATKGTRALEATTEHIRQELAVFCSPEPPAKTSTPEDFIRMTKGITMATAKA
                     VAAGNSCRQEDVIATANLSRRAIADMLRACKEAAFHPEVAPDVRLRALHYGRECANGYL
                     ELLDHVLLTLQKPNPDLKQQLTGHSKRVAGSVTELIQAAEAMKGTEWVDPEDPTVIAEN
                     ELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAA
                     QRELVAQGKVGAIPANALDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASQEKLI
                     SSAKQVAASTAQLLVACKVKADQDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFEDQE
                     NETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELRDEH"
     CDS             6990..7700
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    7841..7962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7932..7951)
                     /label=EBV
                     /note="color: black; sequence: GATGAGTTTGGACAAACCAC"
     rep_origin      complement(7969..8424)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        8451..8555
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        8557..8914
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      8765..8900
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             8949..9743
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    9975..10022
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
     rep_origin      10351..10939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtgccac catgggccgt ggcgtgcctc atatcaccat gacagacgcc tacaagcgtt
      661 acaagcagca gtacaaccga gtggggaaag tggagcacgg ctctgtggct ctgccagcca
      721 tcatgcgctc tggagcctct ggtcctgaga atttccaggt ggggagcatg ccacctgccc
      781 agcagcagat caccagtggc cagatgcacc gaggacacat gccacctctg acttcagccc
      841 agcaggcgct cactggaacc attaactcca gcatgcaggc tgtgcaggct gcccaggcca
      901 ctctggatga ctttgagact ctaccccctc ttggccagga tgctgcctcc aaagcttggc
      961 gtaagaacaa gatggatgaa tcaaagcatg agatccactc ccaggtagat gccatcacag
     1021 ctggcactgc ctctgtggta aacctgacag caggagatcc tgcagagaca gactatacag
     1081 cagtgggctg tgcagtcacc accatctctt ccaacctgac ggagatgtcc cgtggggtga
     1141 agctattggc tgccctgctg gaggatgaag gcggcaatgg ccggcccctt ctgcaagcag
     1201 caaagggcct tgcaggggct gtgtcggaac tgcttcgcag tgctcagccc gccagcgctg
     1261 agcctcgtca gaacctgctg caggcagccg ggaacgtggg ccaggccagt ggggagctgt
     1321 tgcagcaaat tggggaaagt gacactgacc cccacttcca ggatgttcta atgcagctag
     1381 caaacgcagt ggcaagtgct gcagccgccc tggtcctcaa ggccaagagt gtggcccagc
     1441 gcacagagga ttccgggctt cagacccaag tgattgctgc agctacgcaa tgtgctctgt
     1501 ccacttccca gctggtggcc tgtaccaagg tggtagcacc tacaatcagc tcacctgtgt
     1561 gccaagaaca gctagtcgag gctgggcgac tggtggccaa agctgtggag ggctgtgtgt
     1621 ctgcctcgca agcagctaca gaagatggac agctgcttcg aggggtagga gcagcagcca
     1681 cggctgtcac ccaggccctc aatgagctgc tgcagcacgt gaaggcccac gccaccggag
     1741 ctgggcctgc cggtcggtat gaccaagcca ctgataccat cctcaccgtc actgaaaaca
     1801 tcttcagctc catgggtgat gctggggaga tggtgcgcca ggcccgcatc ctggcccaag
     1861 ccacctcaga cctggtcaat gctatcaagg ccgatgctga gggggagagt gatctggaga
     1921 actctagaaa gctcctgagt gctgccaaga tcctcgctga tgccaccgcc aagatggtgg
     1981 aggcggccaa gggagcagcc gcccaccctg acagtgagga acagcagcag cgactgcgtg
     2041 aagcagctga ggggcttcgc atggccacca atgcagctgc gcagaacgcc atcaagaaga
     2101 agttggtgca gcgcctggag catgcagcca agcaagctgc agcctctgca acacagacca
     2161 ttgctgcagc ccaacatgca gcctctgccc ccaaggcctc tgcgggcccc cagcccctac
     2221 tggtgcagag ctgtaaggcc gtggcagagc agattccgtt gctggtgcag ggtgtccgag
     2281 gaagtcaagc tcagcctgac agccctagtg ctcagctcgc cctcattgct gccagccaga
     2341 gcttcctgca gccaggtggg aagatggtgg cagcggcaaa agcctcagtg ccaacaattc
     2401 aggaccaggc ttcagcgatg cagctgagtc agtgcgcaaa gaacctaggc actgccctgg
     2461 ccgagctgcg cactgctgct cagaaggctc aggaggcatg tggacctttg gagatggatt
     2521 ctgcactgag tgttgtacaa aatctagaga aagacctaca ggaaataaaa gcagcagctc
     2581 gagatggcaa gcttaagccc ttacctgggg aaacaatgga gaagtgtacc caagatcttg
     2641 gcaacagcac caaagcggtg agctctgcca tcgccaagct gctgggggag attgcccagg
     2701 gcaacgagaa ctatgcaggt attgcagctc gggatgtagc tggtggacta aggtcactag
     2761 cccaggctgc gcggggtgtg gctgcgctga catcagatcc tgcagtgcag gccattgtgc
     2821 ttgacacagc cagcgacgtt ctggacaagg ccagcagcct cattgaagag gcaaaaaaag
     2881 catctggaca cccaggggac ccagaaagcc agcaaaggct tgctcaggta gctaaagcag
     2941 tgacccaagc cctgaaccgc tgtgtcagct gcctgcctgg ccaaagagac gtggataatg
     3001 ccctacgagc cgttggagat gccagcaagc gactcctgag tgacttgctt cctccaagca
     3061 cggggacatt tcaagaagca cagagccgat tgaatgaagc tgcagctggg ttaaatcagg
     3121 ccgccacaga actggtgcaa gcctcccgag gaactcctca ggacctggct cgggcctccg
     3181 gccgatttgg acaggacttc agcaccttcc tggaagctgg tgtggagatg gccggacagg
     3241 ccccgagcca ggaggaccga gcccaggtgg tgtccaacct gaagggcata tccatgtctt
     3301 caagcaaact tctccttgcc gctaaggcct tgtccacaga ccctgcttct cccaacctca
     3361 agagtcagct ggctgcggct gcccgggcag tgacggacag catcaaccag ctcatcacca
     3421 tgtgcaccca gcaggcacct ggccagaagg agtgtgacaa tgcacttcgg cagctagaga
     3481 cagtccgaga actcctggag aatccagtcc agcccatcaa cgacatgtcc tacttcggtt
     3541 gcttggacag tgtcatggag aactccaagg tcctaggtga ggccatgact ggcatctccc
     3601 aaaatgccaa gaatggaaat ctgccggagt ttggagatgc cattgccaca gcctccaagg
     3661 ctctctgtgg cttcacggag gcagccgcac aggcagcata tctagttggt gtctctgacc
     3721 ccaacagcca agctggacag caaggactgg tggaacccac acagtttgcc cgtgcaaacc
     3781 aggcaattca gatggcctgt cagagtctgg gggagcctgg ctgtacccag gcccaggtgt
     3841 tatctgcagc cactattgta gccaaacaca catctgcatt gtgtaacagc tgtcgcctgg
     3901 cttccgctag gactgccaat cccactgcca agcgccagtt tgtacagtca gccaaggagg
     3961 tggccaacag tacagccaat cttgtcaaga ccatcaaggc actagatggg gacttcacag
     4021 aagagaaccg tgcccagtgc cgagcagcca cagcccctct gctggaagct gtggataacc
     4081 tgagtgcctt tgcctccaac cctgagttct ccagcgtccc tgcccagatc agccctgagg
     4141 gcagagcagc catggagccc attgtaatct ctgctaagac aatgttggag agtgctggag
     4201 ggctcatcca gacagctcgg gcgttagcag tcaatccccg agacccccca cgttggtctg
     4261 tgctagctgg ccactctcgg actgtctcag attccatcaa gaaacttatt acaagcatga
     4321 gggacaaagc cccagggcag ctggagtgtg agacagccat tgcggctctg aacagctgct
     4381 tgcgggacct agatcaggct tcgcttgccg ctgtcagcca gcagcttgct ccccgtgaag
     4441 gaatctctca agaggctttg cacacccaga tgctcactgc agtgcaagaa atttctcatc
     4501 tcattgagcc gctggccagc gctgctcggg ctgaagcctc ccagctggga cacaaggtgt
     4561 cccaaatggc ccagtacttt gaaccactca ccctggctgc agtgggtgct gcgtctaaga
     4621 ccctgagcca cccgcagcag atggcacttc tggaccagac taaaacgttg gcagagtctg
     4681 ccttgcagtt gctatatact gccaaggagg ctggtggtaa ccccaagcaa gcagcacaca
     4741 cccaggaagc cctggaggag gctgtgcaga tgatgacaga ggccgtagaa gacctgacga
     4801 cgaccctcaa cgaagcagcc agtgctgcag gagtcgttgg cggcatggtg gactctatca
     4861 ctcaggccat caaccagcta gatgaaggac ctatgggtga cccagaaggc tcattcgtag
     4921 attaccagac aaccatggtg aggacagcca aggccattgc tgtcactgtt caggagatgg
     4981 taaccaagtc aaacaccagc cctgaagagc taggccctct tgccaaccag ctgaccagtg
     5041 actatggccg actggcctca caagccaagc ctgcagctgt ggctgctgaa aatgaagaga
     5101 taggcgctca catcaaacac cgagtacagg agctgggcca tggctgctct gctctggtca
     5161 ccaaggcagg tgccttgcag tgtagcccca gtgatgtcta caccaagaag gagctcatag
     5221 agtgtgcccg cagagtgtca gagaaggtct cccatgttct ggctgcactc caggctggga
     5281 atcgtggtac ccaggcctgc attacagcag ccagtgctgt gtctggtatc attgctgacc
     5341 tcgacaccac catcatgttc gctactgctg gcacacttaa ccgtgagggt gctgaaactt
     5401 ttgctgacca ccgggagggt atcttaaaga cagcaaaggt tcttgtggag gacaccaagg
     5461 tcctagtgca gaatgcagct gggagccagg agaagttggc acaagccgcc cagtcctccg
     5521 tggccaccat tacccgcctc gctgatgtgg tcaagctcgg tgcagccagc ctaggagccg
     5581 aagaccctga aactcaggtg gtgctgatca atgcagtaaa ggacgtagcc aaggccctgg
     5641 gtgacctcat cagcgctacg aaggctgcag cgggcaaagt tggggatgac cctgcagtgt
     5701 ggcagctcaa gaactctgcc aaggtgatgg tgaccaatgt gacatcattg ctcaagacag
     5761 tgaaggctgt ggaagatgag gccaccaaag gcacacgggc cctagaggca accacagagc
     5821 acatacgtca ggaactggcg gtcttctgtt ccccagagcc acctgccaag acctctaccc
     5881 ctgaagactt catccgaatg accaagggta ttactatggc aacagccaaa gccgttgctg
     5941 ctggcaattc ctgtcgacag gaagatgtca ttgccacagc caatctgagc cgacgggcta
     6001 ttgcggacat gcttcgggct tgcaaggaag cagctttcca cccagaagtg gcgcctgatg
     6061 tacggctccg agccctgcac tatggccggg agtgtgccaa tggttacctg gaactgctgg
     6121 accacgtgct gctgaccctt cagaagccaa acccagacct gaagcagcag ctaactggac
     6181 actcaaagcg ggttgctggc tctgtgactg agctcatcca ggctgctgaa gccatgaaag
     6241 gaacagagtg ggtggaccca gaggacccta ctgtcattgc tgagaatgag ctcttgggag
     6301 ccgcagccgc catcgaggct gcagccaaga agctggagca gctgaagccc agggccaaac
     6361 ccaaggaggc ggatgagtcc ttgaactttg aggaacaaat cctagaagct gccaagtcca
     6421 tcgctgcagc caccagtgca ctggtaaagg ctgcgtcagc agcccagagg gagctggtgg
     6481 ctcaaggaaa ggtgggcgcc attccagcca atgcactgga tgatgggcag tggtcgcagg
     6541 gcctcatttc cgctgcccgt atggtggctg cggccaccaa caatctgtgt gaagcagcta
     6601 acgcagctgt ccagggccat gctagccagg agaaactcat ttcctcagcc aagcaagtag
     6661 ctgcctccac agctcagctc ctggtagctt gcaaggtcaa agcagatcag gactctgagg
     6721 caatgaaacg gctccaggct gctggcaatg cagtgaagag ggcctcagat aacctggtaa
     6781 aggcggccca gaaagctgca gccttcgaag accaggagaa tgagacggtg gtggtgaagg
     6841 agaaaatggt tgggggcatt gcccagatta tcgcagcaca ggaagagatg cttcggaagg
     6901 aacgagagct ggaagaggct cggaaaaagc tcgcccagat ccggcagcag cagtacaagt
     6961 tcttgccttc agagcttcga gacgagcaca tggtgagcaa gggcgaggag gataacatgg
     7021 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     7081 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     7141 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     7201 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     7261 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     7321 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     7381 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     7441 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     7501 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     7561 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     7621 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     7681 tggacgagct gtacaagtaa ccgcgggccc gggatccacc ggatctagat aactgatcat
     7741 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     7801 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     7861 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7921 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     7981 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     8041 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     8101 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     8161 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     8221 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     8281 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     8341 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     8401 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8461 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8521 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     8581 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8641 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8701 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8761 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8821 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8881 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     8941 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     9001 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     9061 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     9121 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     9181 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     9241 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     9301 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     9361 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     9421 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     9481 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     9541 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     9601 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     9661 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     9721 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     9781 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     9841 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     9901 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     9961 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
    10021 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
    10081 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
    10141 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
    10201 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    10261 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    10321 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    10381 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    10441 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    10501 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10561 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10621 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10681 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10741 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10801 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10861 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10921 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    10981 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    11041 taccgccatg cat
//
