LOCUS       mTB3-Flag_tag        8273 bp DNA     circular SYN 03-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 3, 2023 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             103..918
                     /codon_start=1
                     /label=KanR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             980..1152
                     /codon_start=1
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=15.974145891043397"
                     /note="/other=CDS"
                     /translation="CCNSLRARR*RAISCCPSHW*KEKPPWRPIRKPPLPARWPIH*CS
                     WHDRFPDWKAGS"
     protein_bind    1165..1186
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
     promoter        1201..1231
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=96.875"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1235..1257
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.83333333333334"
                     /note="/other=protein bind"
     protein_bind    1239..1255
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1263..1279
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1275..1781
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVV
                     VPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFD
                     GVPIG"
     misc_feature    1289..1345
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1349..1365)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1770..1902
                     /direction=RIGHT
                     /label=M13 ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=99.248"
                     /note="/match_length=26.078431372549023"
                     /note="/other=rep_origin"
     CDS             1907..3139
                     /codon_start=1
                     /label=M13 gene II
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.919"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGINLSAR
                     TVEYHIDGDLTVSGLSHPFESLPTHYSCIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
                     FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
                     RNVSNGQTKSTRSQNWESTVIWNETSRHRTLVAYLKHVELQHYIQQLSSKPSAKMTSYQ
                     KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
                     DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
                     VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
                     DWYNEPVLKIA"
     CDS             3151..3414
                     /codon_start=1
                     /label=M13 gene V
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
                     KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             3416..3517
                     /codon_start=1
                     /label=M13 gene VII
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             3514..3612
                     /codon_start=1
                     /label=M13 gene IX
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     CDS             3609..3830
                     /codon_start=1
                     /label=M13 gene VIII
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEYI
                     GYAWAMVVVIVGATIGIKLFKKFTSKAS"
     terminator      3834..3881
                     /label=fd terminator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             3886..3948
                     /codon_start=1
                     /label=M13 protein III signal sequence
                     /translation="VKKLLFAIPLVVPFYSHSAMA"
     CDS             3952..3975
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=Flag-tag
                     /translation="DYKDDDDK"
     CDS             3984..4182
                     /codon_start=1
                     /label=M13 protein III N1 domain
                     /translation="TVESCLAKSHTENSFTNVWKDDKTLDCYANYEGCLWNATGVVVCT
                     GDETQCYGTWVPIGLAIPENE"
     CDS             4183..4236
                     /codon_start=1
                     /label=Linker 1
                     /translation="GGGSEGGGSEGGGSEGGG"
     CDS             4237..4629
                     /codon_start=1
                     /label=M13 protein III N2 domain
                     /translation="TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPL
                     NTFMFQNNRFRNRQGALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAF
                     HSGFNEDLFVCEYQGQSSDLPQPPVNA"
     CDS             4630..4746
                     /codon_start=1
                     /label=Linker 2
                     /translation="GGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSG"
     CDS             4747..5115
                     /codon_start=1
                     /label=M13 protein III CT domain
                     /translation="DFDYEKMANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDG
                     FIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFG
                     AGKPYEFSIDCDKINLFRG"
     CDS             5116..5178
                     /codon_start=1
                     /label=M13 protein III transmembrane domain
                     /translation="VFAFLLYVATFMYVFSTFANI"
     CDS             5179..5196
                     /codon_start=1
                     /label=M13 pIII exposed tail
                     /translation="LRNKES"
     CDS             5202..5540
                     /codon_start=1
                     /label=M13 gene VI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     CDS             5542..6588
                     /codon_start=1
                     /label=M13 gene I
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
                     VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
                     QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
                     ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
                     TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
                     SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
                     SNEIVKCN"
     CDS             6566..7846
                     /codon_start=1
                     /label=M13 gene IV
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     rep_origin      7834..8214
                     /direction=RIGHT
                     /label=M13 ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             8215..8262
                     /codon_start=1
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=4.444444444444445"
                     /note="/other=CDS"
                     /translation="NHHQTGFSPAGANQRG"
ORIGIN
        1 gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
       61 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
      121 gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
      181 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
      241 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
      301 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
      361 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca
      421 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
      481 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
      541 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
      601 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg
      661 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
      721 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
      781 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
      841 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
      901 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
      961 ctgacttgac gggacggcgt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     1021 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     1081 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1141 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     1201 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     1261 cacaggaaac agctatgacc atgattacga attcgagctc ggtacccggg gatcctctag
     1321 agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
     1381 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     1441 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     1501 aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg
     1561 atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg
     1621 cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg
     1681 agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag
     1741 gccagacgcg aattattttt gatggcgttc ctattggtta aaaaatgagc tgatttaaca
     1801 aaaatttaat gcgaatttta acaaaatatt aacgtttaca atttaaatat ttgcttatac
     1861 aatcttcctg tttttggggc ttttctgatt atcaaccggg gtacatatga ttgacatgct
     1921 agttttacga ttaccgttca tcgattctct tgtttgctcc agactctcag gcaatgacct
     1981 gatagccttt gtagatctct caaaaatagc taccctctcc ggcattaatt tatcagctag
     2041 aacggttgaa tatcatattg atggtgattt gactgtctcc ggcctttctc acccttttga
     2101 atctttacct acacattact catgcattgc atttaaaata tatgagggtt ctaaaaattt
     2161 ttatccttgc gttgaaataa aggcttctcc cgcaaaagta ttacagggtc ataatgtttt
     2221 tggtacaacc gatttagctt tatgctctga ggctttattg cttaattttg ctaattcttt
     2281 gccttgcctg tatgatttat tggatgttaa tgctactact attagtagaa ttgatgccac
     2341 cttttcagct cgcgccccaa atgaaaatat agctaaacag gttattgacc atttgcgaaa
     2401 tgtatctaat ggtcaaacta aatctactcg ttcgcagaat tgggaatcaa ctgttatatg
     2461 gaatgaaact tccagacacc gtactttagt tgcatattta aaacatgttg agctacagca
     2521 ttatattcag caattaagct ctaagccatc cgcaaaaatg acctcttatc aaaaggagca
     2581 attaaaggta ctctctaatc ctgacctgtt ggagtttgct tccggtctgg ttcgctttga
     2641 agctcgaatt aaaacgcgat atttgaagtc tttcgggctt cctcttaatc tttttgatgc
     2701 aatccgcttt gcttctgact ataatagtca gggtaaagac ctgatttttg atttatggtc
     2761 attctcgttt tctgaactgt ttaaagcatt tgagggggat tcaatgaata tttatgacga
     2821 ttccgcagta ttggacgcta tccagtctaa acattttact attaccccct ctggcaaaac
     2881 ttcttttgca aaagcctctc gctattttgg tttttatcgt cgtctggtaa acgagggtta
     2941 tgatagtgtt gctcttacta tgcctcgtaa ttccttttgg cgttatgtat ctgcattagt
     3001 tgaatgtggt attcctaaat ctcaactgat gaatctttct acctgtaata atgttgttcc
     3061 gttagttcgt tttattaacg tagatttttc ttcccaacgt cctgactggt ataatgagcc
     3121 agttcttaaa atcgcataag gtaattcaca atgattaaag ttgaaattaa accatctcaa
     3181 gcccaattta ctactcgttc tggtgtttct cgtcagggca agccttattc actgaatgag
     3241 cagctttgtt acgttgattt gggtaatgaa tatccggttc ttgtcaagat tactcttgat
     3301 gaaggtcagc cagcctatgc gcctggtctg tacaccgttc atctgtcctc tttcaaagtt
     3361 ggtcagttcg gttcccttat gattgaccgt ctgcgcctcg ttccggctaa gtaacatgga
     3421 gcaggtcgcg gatttcgaca caatttatca ggcgatgata caaatctccg ttgtactttg
     3481 tttcgcgctt ggtataatcg ctgggggtca aagatgagtg ttttagtgta ttcttttgcc
     3541 tctttcgttt taggttggtg ccttcgtagt ggcattacgt attttacccg tttaatggaa
     3601 acttcctcat gaaaaagtct ttagtcctca aagcctctgt agccgttgct accctcgttc
     3661 cgatgctgtc tttcgctgct gagggtgacg atcccgcaaa agcggccttt aactccctgc
     3721 aagcctcagc gaccgaatat atcggttatg cgtgggcgat ggttgttgtc attgtcggcg
     3781 caactatcgg tatcaagctg tttaagaaat tcacctcgaa agcaagctga taaaccgata
     3841 caattaaagg ctccttttgg agcctttttt ttggagattt tcaacgtgaa aaaattatta
     3901 ttcgcaattc ctttagttgt tcctttctat tctcactccg ctatggctgc ggactacaag
     3961 gacgacgatg acaagcttgc agaaactgtt gaaagttgtt tagcaaaatc ccatacagaa
     4021 aattcattta ctaacgtctg gaaagacgac aaaactttag attgttacgc taactatgag
     4081 ggctgtctgt ggaatgctac aggcgttgta gtttgtactg gtgacgaaac tcagtgttac
     4141 ggtacatggg ttcctattgg gcttgctatc cctgaaaatg agggtggtgg ctctgagggt
     4201 ggcggttctg agggtggcgg ttctgagggt ggcggtacta aacctcctga gtacggtgat
     4261 acacctattc cgggctatac ttatatcaac cctctcgacg gcacttatcc gcctggtact
     4321 gagcaaaacc ccgctaatcc taatccttct cttgaggagt ctcagcctct taatactttc
     4381 atgtttcaga ataataggtt ccgaaatagg cagggggcat taactgttta tacgggcact
     4441 gttactcaag gcactgaccc cgttaaaact tattaccagt acactcctgt atcatcaaaa
     4501 gccatgtatg acgcttactg gaacggtaaa ttcagagact gcgctttcca ttctggcttt
     4561 aatgaggatt tatttgtttg tgaatatcaa ggccaatcgt ctgacctgcc tcaacctcct
     4621 gtcaatgctg gcggcggctc tggtggtggt tctggtggcg gctctgaggg tggtggctct
     4681 gagggtggcg gttctgaggg tggcggctct gagggaggcg gttccggtgg tggctctggt
     4741 tccggtgatt ttgattatga aaagatggca aacgctaata agggggctat gaccgaaaat
     4801 gccgatgaaa acgcgctaca gtctgacgct aaaggcaaac ttgattctgt cgctactgat
     4861 tacggtgctg ctatcgatgg tttcattggt gacgtttccg gccttgctaa tggtaatggt
     4921 gctactggtg attttgctgg ctctaattcc caaatggctc aagtcggtga cggtgataat
     4981 tcacctttaa tgaataattt ccgtcaatat ttaccttccc tccctcaatc ggttgaatgt
     5041 cgcccttttg tctttggcgc tggtaaacca tatgaatttt ctattgattg tgacaaaata
     5101 aacttattcc gtggtgtctt tgcgtttctt ttatatgttg ccacctttat gtatgtattt
     5161 tctacgtttg ctaacatact gcgtaataag gagtcttaat catgccagtt cttttgggta
     5221 ttccgttatt attgcgtttc ctcggtttcc ttctggtaac tttgttcggc tatctgctta
     5281 cttttcttaa aaagggcttc ggtaagatag ctattgctat ttcattgttt cttgctctta
     5341 ttattgggct taactcaatt cttgtgggtt atctctctga tattagcgct caattaccct
     5401 ctgactttgt tcagggtgtt cagttaattc tcccgtctaa tgcgcttccc tgtttttatg
     5461 ttattctctc tgtaaaggct gctattttca tttttgacgt taaacaaaaa atcgtttctt
     5521 atttggattg ggataaataa tatggctgtt tattttgtaa ctggcaaatt aggctctgga
     5581 aagacgctcg ttagcgttgg taagattcag gataaaattg tagctgggtg caaaatagca
     5641 actaatcttg atttaaggct tcaaaacctc ccgcaagtcg ggaggttcgc taaaacgcct
     5701 cgcgttctta gaataccgga taagccttct atatctgatt tgcttgctat tgggcgcggt
     5761 aatgattcct acgatgaaaa taaaaacggc ttgcttgttc tcgatgagtg cggtacttgg
     5821 tttaataccc gttcttggaa tgataaggaa agacagccga ttattgattg gtttctacat
     5881 gctcgtaaat taggatggga tattattttt cttgttcagg acttatctat tgttgataaa
     5941 caggcgcgtt ctgcattagc tgaacatgtt gtttattgtc gtcgtctgga cagaattact
     6001 ttaccttttg tcggtacttt atattctctt attactggct cgaaaatgcc tctgcctaaa
     6061 ttacatgttg gcgttgttaa atatggcgat tctcaattaa gccctactgt tgagcgttgg
     6121 ctttatactg gtaagaattt gtataacgca tatgatacta aacaggcttt ttctagtaat
     6181 tatgattccg gtgtttattc ttatttaacg ccttatttat cacacggtcg gtatttcaaa
     6241 ccattaaatt taggtcagaa gatgaaatta actaaaatat atttgaaaaa gttttctcgc
     6301 gttctttgtc ttgcgattgg atttgcatca gcatttacat atagttatat aacccaacct
     6361 aagccggagg ttaaaaaggt agtctctcag acctatgatt ttgataaatt cactattgac
     6421 tcttctcagc gtcttaatct aagctatcgc tatgttttca aggattctaa gggaaaatta
     6481 attaatagcg acgatttaca gaagcaaggt tattcactca catatattga tttatgtact
     6541 gtttccatta aaaaaggtaa ttcaaatgaa attgttaaat gtaattaatt ttgttttctt
     6601 gatgtttgtt tcatcatctt cttttgctca ggtaattgaa atgaataatt cgcctctgcg
     6661 cgattttgta acttggtatt caaagcaatc aggcgaatcc gttattgttt ctcccgatgt
     6721 aaaaggtact gttactgtat attcatctga cgttaaacct gaaaatctac gcaatttctt
     6781 tatttctgtt ttacgtgcaa ataattttga tatggtaggt tctaaccctt ccattattca
     6841 gaagtataat ccaaacaatc aggattatat tgatgaattg ccatcatctg ataatcagga
     6901 atatgatgat aattccgctc cttctggtgg tttctttgtt ccgcaaaatg ataatgttac
     6961 tcaaactttt aaaattaata acgttcgggc aaaggattta atacgagttg tcgaattgtt
     7021 tgtaaagtct aatacttcta aatcctcaaa tgtattatct attgacggct ctaatctatt
     7081 agttgttagt gctcctaaag atattttaga taaccttcct caattccttt caactgttga
     7141 tttgccaact gaccagatat tgattgaggg tttgatattt gaggttcagc aaggtgatgc
     7201 tttagatttt tcatttgctg ctggctctca gcgtggcact gttgcaggcg gtgttaatac
     7261 tgaccgcctc acctctgttt tatcttctgc tggtggttcg ttcggtattt ttaatggcga
     7321 tgttttaggg ctatcagttc gcgcattaaa gactaatagc cattcaaaaa tattgtctgt
     7381 gccacgtatt cttacgcttt caggtcagaa gggttctatc tctgttggcc agaatgtccc
     7441 ttttattact ggtcgtgtga ctggtgaatc tgccaatgta aataatccat ttcagacgat
     7501 tgagcgtcaa aatgtaggta tttccatgag cgtttttcct gttgcaatgg ctggcggtaa
     7561 tattgttctg gatattacca gcaaggccga tagtttgagt tcttctactc aggcaagtga
     7621 tgttattact aatcaaagaa gtattgctac aacggttaat ttgcgtgatg gacagactct
     7681 tttactcggt ggcctcactg attataaaaa cacttctcag gattctggcg taccgttcct
     7741 gtctaaaatc cctttaatcg gcctcctgtt tagctcccgc tctgattcta acgaggaaag
     7801 cacgttatac gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     7861 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7921 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7981 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     8041 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     8101 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     8161 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggaaccac
     8221 catcaaacag gattttcgcc tgctggggca aaccagcgtg gagggaaagc cac
//
