LOCUS       pUdO1a        2043 bp DNA     circular SYN 28-SEP-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     multiple        1..899
                     /label=MCS1
                     /note="color: #a6acb3"
     origin          1..629
                     /label=colE1
                     /note="color: #a6acb3"
     misc_feature    630..986
                     /label=MCS1_part
                     /note="color: #a6acb3"
     primer_bind     630..650
                     /label=M13-rev
                     /note="color: #a020f0; direction: RIGHT"
     terminator      668..725
                     /label=thrLABC_ter
                     /note="color: #ffffff"
     terminator      900..960
                     /label=L3S2P21_ter
                     /note="color: #ffffff"
     terminator      900..960
                     /label=L3S2P21_ter
                     /note="color: #ffffff"
     primer_bind     complement(969..986)
                     /label=M13-fwd
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    987..1015
                     /label=AmpR promoter
                     /note="color: #a6acb3"
     CDS             1057..1914
                     /codon_start=1
                     /label=AmpR
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1918..1937
                     /label=T7
                     /note="color: #a020f0; direction: RIGHT"
     terminator      1957..2010
                     /label=arcA_ter
                     /note="color: #ffffff"
     promoter        complement(2025..2043)
                     /label=T3 promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctttgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggccc aggaaacagc tatgaccatg cccgggtaag
      661 tgactagcgg aaacacagaa aaaagcccgc acctgacagt gcgggctttt tttttcgacc
      721 aaaggctccg aagagccgag tgtcttcggt tcgagaccga attcgcgagc tctcgctagc
      781 cagtttaaac aattgcggcc gcgtactagt ctatcgattt ggatccaccc tgcaggaagt
      841 ctagaccgtc gacacggtct ctgagagaag acacgtcgct cttcgacgta agtaactgac
      901 tcggtaccaa attccagaaa agaggcctcc cgaaaggggg gccttttttc gttttggtcc
      961 taagcttaac tggccgtcgt tttacattca aatatgtatc cgctcatgag acaataaccc
     1021 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1081 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1141 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1201 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1261 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     1321 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1381 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1441 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1501 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     1561 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     1621 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     1681 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     1741 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     1801 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     1861 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaataa
     1921 tacgactcac tatagggtaa ctaattgagg cgcgcctacc accgtcaaaa aaaacggcgc
     1981 tttttagcgc cgtttttatt tttcaacctt cctaggtaag tgacccttta gtgagggtta
     2041 att
//
