LOCUS       pUdO3a_sequenced        2635 bp DNA     circular SYN 30-NOV-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2023 from SnapGene Viewer 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     origin          1..1221
                     /label=pSC101
                     /note="color: #faac61"
     misc_feature    1..223
                     /label=ori SC101
                     /note="color: #9eafd2"
     primer_bind     1222..1242
                     /label=M13-rev
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      1260..1317
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     misc_feature    1318..1491
                     /label=MCS1
                     /note="color: #b4abac"
     terminator      1492..1552
                     /label=L3S2P21_ter
                     /note="color: #b1ff67"
     primer_bind     complement(1561..1578)
                     /label=M13-fwd
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    1579..1607
                     /label=AmpR promoter
                     /note="color: #84b0dc"
     CDS             1649..2506
                     /label=AmpR
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     2510..2529
                     /label=T7
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      2549..2602
                     /label=arcA_ter
                     /note="color: #b1ff67"
     promoter        complement(2617..2635)
                     /label=T3 promoter
                     /note="color: #ff9ccd; direction: LEFT"
ORIGIN
        1 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
       61 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
      121 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
      181 acaactcaaa ggaaaaggac atgtaattat cattgactag cccatctcaa ttggtatagt
      241 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
      301 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
      361 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
      421 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
      481 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
      541 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
      601 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
      661 ctggaacatg ttaagtcttt tgaaaacaaa tactccatga ggatttatga gtggttatta
      721 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
      781 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
      841 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
      901 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
      961 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     1021 acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc tttaactgca
     1081 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagcatgat
     1141 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa caacactaga gaacatactg
     1201 gctaaatacg gaaggatctg acaggaaaca gctatgacca tgcccgggta agtgactagc
     1261 ggaaacacag aaaaaagccc gcacctgaca gtgcgggctt tttttttcga ccaaaggctc
     1321 cgaagagccg agtgtcttcg gttcgagacc gaattcgcga gctctcgcta gccagtttaa
     1381 acaattgcgg ccgcgtacta gtctatcgat ttggatccac cctgcaggaa gtctagaccg
     1441 tcgacacggt ctctgagaga agacacgtcg ctcttcgacg taagtaactg actcggtacc
     1501 aaattccaga aaagaggcct cccgaaaggg gggccttttt tcgttttggt cctaagctta
     1561 actggccgtc gttttacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1621 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1681 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1741 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1801 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1861 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1921 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1981 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2041 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2101 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     2161 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     2221 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     2281 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     2341 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     2401 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     2461 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaat aatacgactc
     2521 actatagggt aactaattga ggcgcgccta ccaccgtcaa aaaaaacggc gctttttagc
     2581 gccgttttta tttttcaacc ttcctaggta agtgaccctt tagtgagggt taatt
//
