LOCUS       pudo4a        2847 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     origin          1..1433
                     /label=pBBR1
                     /note="color: #a6acb3"
     misc_feature    1..770
                     /label=oriV
                     /note="color: #a6acb3"
     primer_bind     596..613
                     /label=SeqbGO1 (bG051)
                     /note="color: black; sequence: GTACGCAACAGCTCATTG"
     primer_bind     624..638
                     /label=SeqbGO1 (bG051)
                     /note="color: black; sequence: GTACGCAACAGCTCATTG"
     misc_feature    771..1433
                     /label=Rep
                     /note="color: #a6acb3"
     CDS             771..1433
                     /codon_start=1
                     /label=Translation 771-1433
                     /note="color: #993366"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     1434..1454
                     /label=M13-rev
                     /note="color: #a020f0; direction: RIGHT"
     terminator      1472..1529
                     /label=thrLABC_ter
                     /note="color: #ffffff"
     terminator      1704..1764
                     /label=L3S2P21_ter
                     /note="color: #ffffff"
     primer_bind     complement(1773..1790)
                     /label=M13-fwd
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    1791..1819
                     /label=AmpR promoter
                     /note="color: #a6acb3"
     CDS             1861..2718
                     /codon_start=1
                     /label=AmpR
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2722..2741
                     /label=T7
                     /note="color: #a020f0; direction: RIGHT"
     terminator      2761..2814
                     /label=arcA_ter
                     /note="color: #ffffff"
     promoter        complement(2829..2847)
                     /label=T3 promoter
                     /note="color: #aa00ff; direction: LEFT"
ORIGIN
        1 gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg
       61 gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt
      121 cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt
      181 aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac
      241 accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc
      301 gcctggaacg acccaagcct atgcgagtgg gggcagtcga aggcgaagcc cgcccgcctg
      361 ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg gggcagcgcc accttgggca
      421 aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg gccacttttt gccggagggg
      481 gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc cttctttggg caccaaagaa
      541 ctagatatag ggcgaaatgc gaaagactta aaaatcaaca acttaaaaaa ggggggtacg
      601 caacagctca ttgcggcacc ccccgcaata gctcattgcg taggttaaag aaaatctgta
      661 attgactgcc acttttacgc aacgcataat tgttgtcgcg ctgccgaaaa gttgcagctg
      721 attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat ggagataagc atggccacgc
      781 agtccagaga aatcggcatt caagccaaga acaagcccgg tcactgggtg caaacggaac
      841 gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa acccacggcg gcaatgctgc
      901 tgcatcacct cgtggcgcag atgggccacc agaacgccgt ggtggtcagc cagaagacac
      961 tttccaagct catcggacgt tctttgcgga cggtccaata cgcagtcaag gacttggtgg
     1021 ccgagcgctg gatctccgtc gtgaagctca acggccccgg caccgtgtcg gcctacgtgg
     1081 tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt gcgcctgtcg gtgttcagtg
     1141 ccgccgtggt ggttgatcac gacgaccagg acgaatcgct gttggggcat ggcgacctgc
     1201 gccgcatccc gaccctgtat ccgggcgagc agcaactacc gaccggcccc ggcgaggagc
     1261 cgcccagcca gcccggcatt ccgggcatgg aaccagacct gccagccttg accgaaacgg
     1321 aggaatggga acggcgcggg cagcagcgcc tgccgatgcc cgatgagccg tgttttctgg
     1381 acgatggcga gccgttggag ccgccgacac gggtcacgct gccgcgccgg tagcaggaaa
     1441 cagctatgac catgcccggg taagtgacta gcggaaacac agaaaaaagc ccgcacctga
     1501 cagtgcgggc tttttttttc gaccaaaggc tccgaagagc cgagtgtctt cggttcgaga
     1561 ccgaattcgc gagctctcgc tagccagttt aaacaattgc ggccgcgtac tagtctatcg
     1621 atttggatcc accctgcagg aagtctagac cgtcgacacg gtctctgaga gaagacacgt
     1681 cgctcttcga cgtaagtaac tgactcggta ccaaattcca gaaaagaggc ctcccgaaag
     1741 gggggccttt tttcgttttg gtcctaagct taactggccg tcgttttaca ttcaaatatg
     1801 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1861 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1921 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1981 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2041 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2101 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2161 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2221 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2281 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2341 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2401 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2461 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2521 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2581 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2641 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2701 tcactgatta agcattggta ataatacgac tcactatagg gtaactaatt gaggcgcgcc
     2761 taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa ccttcctagg
     2821 taagtgaccc tttagtgagg gttaatt
//
