LOCUS       pudo1c        1867 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     origin          1..629
                     /label=colE1
                     /note="color: #a6acb3"
     primer_bind     630..650
                     /label=M13-rev
                     /note="color: #a020f0; direction: RIGHT"
     terminator      668..725
                     /label=thrLABC_ter
                     /note="color: #ffffff"
     terminator      900..960
                     /label=L3S2P21_ter
                     /note="color: #ffffff"
     primer_bind     complement(969..986)
                     /label=M13-fwd
                     /note="color: #a020f0; direction: LEFT"
     CDS             1082..1738
                     /codon_start=1
                     /label=CmR
                     /note="color: #993366"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1266..1287
                     /label=pUdO1c 1266-1287 FWD 3
                     /note="color: black; sequence: TTCTTGCCCGCCTGATGAATGC"
     primer_bind     1266..1287
                     /label=pUdO1c 1266-1287 FWD 2
                     /note="color: black; sequence: TTCTTGCCCGCCTGATGAATGC"
     primer_bind     1408..1429
                     /label=pUdO1c 1408-1429 FWD 1
                     /note="color: black; sequence: CCACGACGATTTCCGGCAGTTT"
     primer_bind     complement(1408..1429)
                     /label=pUdO1c 1408-1429 REV 2
                     /note="color: black; sequence: AAACTGCCGGAAATCGTCGTGG"
     primer_bind     complement(1444..1465)
                     /label=pUdO1c 1444-1465 REV 3
                     /note="color: black; sequence: ACCGTAACACGCCACATCTTGC"
     primer_bind     complement(1514..1535)
                     /label=pUdO1c 1514-1535 REV 1
                     /note="color: black; sequence: TCACCCAGGGATTGGCTGAGAC"
     primer_bind     1742..1761
                     /label=T7
                     /note="color: #a020f0; direction: RIGHT"
     terminator      1781..1834
                     /label=arcA_ter
                     /note="color: #ffffff"
     promoter        complement(1849..1867)
                     /label=T3 promoter
                     /note="color: #aa00ff; direction: LEFT"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggccc aggaaacagc tatgaccatg cccgggtaag
      661 tgactagcgg aaacacagaa aaaagcccgc acctgacagt gcgggctttt tttttcgacc
      721 aaaggctccg aagagccgag tgtcttcggt tcgagaccga attcgcgagc tctcgctagc
      781 cagtttaaac aattgcggcc gcgtactagt ctatcgattt ggatccaccc tgcaggaagt
      841 ctagaccgtc gacacggtct ctgagagaag acacgtcgct cttcgacgta agtaactgac
      901 tcggtaccaa attccagaaa agaggcctcc cgaaaggggg gccttttttc gttttggtcc
      961 taagcttaac tggccgtcgt tttacaacgt aagaggttcc aactttcacc ataatgaaat
     1021 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
     1081 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     1141 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     1201 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     1261 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
     1321 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1381 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1441 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1501 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1561 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1621 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1681 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     1741 ataatacgac tcactatagg gtaactaatt gaggcgcgcc taccaccgtc aaaaaaaacg
     1801 gcgcttttta gcgccgtttt tatttttcaa ccttcctagg taagtgaccc tttagtgagg
     1861 gttaatt
//
