LOCUS       pudo4c        2671 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     origin          1..1433
                     /label=pBBR1
                     /note="color: #f58a5e"
     primer_bind     1434..1454
                     /label=M13-rev
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      1472..1529
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     misc_feature    1530..1703
                     /label=MCS
                     /note="color: #a6acb3"
     terminator      1704..1764
                     /label=L3S2P21_ter
                     /note="color: #b1ff67"
     primer_bind     complement(1773..1790)
                     /label=M13-fwd
                     /note="color: #ff9ccd; direction: LEFT"
     CDS             1886..2542
                     /label=CmR
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     2546..2565
                     /label=T7
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      2585..2638
                     /label=arcA_ter
                     /note="color: #b1ff67"
     promoter        complement(2653..2671)
                     /label=T3 promoter
                     /note="color: #ff9ccd; direction: LEFT"
ORIGIN
        1 gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg
       61 gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt
      121 cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt
      181 aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac
      241 accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc
      301 gcctggaacg acccaagcct atgcgagtgg gggcagtcga aggcgaagcc cgcccgcctg
      361 ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg gggcagcgcc accttgggca
      421 aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg gccacttttt gccggagggg
      481 gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc cttctttggg caccaaagaa
      541 ctagatatag ggcgaaatgc gaaagactta aaaatcaaca acttaaaaaa ggggggtacg
      601 caacagctca ttgcggcacc ccccgcaata gctcattgcg taggttaaag aaaatctgta
      661 attgactgcc acttttacgc aacgcataat tgttgtcgcg ctgccgaaaa gttgcagctg
      721 attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat ggagataagc atggccacgc
      781 agtccagaga aatcggcatt caagccaaga acaagcccgg tcactgggtg caaacggaac
      841 gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa acccacggcg gcaatgctgc
      901 tgcatcacct cgtggcgcag atgggccacc agaacgccgt ggtggtcagc cagaagacac
      961 tttccaagct catcggacgt tctttgcgga cggtccaata cgcagtcaag gacttggtgg
     1021 ccgagcgctg gatctccgtc gtgaagctca acggccccgg caccgtgtcg gcctacgtgg
     1081 tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt gcgcctgtcg gtgttcagtg
     1141 ccgccgtggt ggttgatcac gacgaccagg acgaatcgct gttggggcat ggcgacctgc
     1201 gccgcatccc gaccctgtat ccgggcgagc agcaactacc gaccggcccc ggcgaggagc
     1261 cgcccagcca gcccggcatt ccgggcatgg aaccagacct gccagccttg accgaaacgg
     1321 aggaatggga acggcgcggg cagcagcgcc tgccgatgcc cgatgagccg tgttttctgg
     1381 acgatggcga gccgttggag ccgccgacac gggtcacgct gccgcgccgg tagcaggaaa
     1441 cagctatgac catgcccggg taagtgacta gcggaaacac agaaaaaagc ccgcacctga
     1501 cagtgcgggc tttttttttc gaccaaaggc tccgaagagc cgagtgtctt cggttcgaga
     1561 ccgaattcgc gagctctcgc tagccagttt aaacaattgc ggccgcgtac tagtctatcg
     1621 atttggatcc accctgcagg aagtctagac cgtcgacacg gtctctgaga gaagacacgt
     1681 cgctcttcga cgtaagtaac tgactcggta ccaaattcca gaaaagaggc ctcccgaaag
     1741 gggggccttt tttcgttttg gtcctaagct taactggccg tcgttttaca acgtaagagg
     1801 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
     1861 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     1921 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     1981 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     2041 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
     2101 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     2161 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     2221 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     2281 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     2341 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     2401 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     2461 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     2521 cgatgagtgg cagggcgggg cgtaataata cgactcacta tagggtaact aattgaggcg
     2581 cgcctaccac cgtcaaaaaa aacggcgctt tttagcgccg tttttatttt tcaaccttcc
     2641 taggtaagtg accctttagt gagggttaat t
//
