LOCUS       pUdO1k        2107 bp DNA     circular SYN 06-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 6, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     origin          1..629
                     /label=colE1
                     /note="color: #f58a5e"
     primer_bind     630..650
                     /label=M13-rev
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      668..725
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     terminator      900..960
                     /label=L3S2P21_ter
                     /note="color: #b1ff67"
     primer_bind     complement(969..986)
                     /label=M13-fwd
                     /note="color: #ff9ccd; direction: LEFT"
     promoter        987..1186
                     /label=promoter_KanR
                     /note="color: #d6b295"
     CDS             1187..1981
                     /codon_start=1
                     /label=KanR
                     /label=Translation 1187-1981
                     /note="color: #993366"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     1982..2001
                     /label=T7
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      2021..2074
                     /label=arcA_ter
                     /note="color: #b1ff67"
     promoter        complement(2089..2107)
                     /label=T3 promoter
                     /note="color: #ff9ccd; direction: LEFT"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggccc aggaaacagc tatgaccatg cccgggtaag
      661 tgactagcgg aaacacagaa aaaagcccgc acctgacagt gcgggctttt tttttcgacc
      721 aaaggctccg aagagccgag tgtcttcggt tcgagaccga attcgcgagc tctcgctagc
      781 cagtttaaac aattgcggcc gcgtactagt ctatcgattt ggatccaccc tgcaggaagt
      841 ctagaccgtc gacacggtct ctgagagaag acacgtcgct cttcgacgta agtaactgac
      901 tcggtaccaa attccagaaa agaggcctcc cgaaaggggg gccttttttc gttttggtcc
      961 taagcttaac tggccgtcgt tttacatagc ttgcagtggg cttacatggc gatagctaga
     1021 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
     1081 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
     1141 caggggatca agatctgatc aagagacagg atgacggtcg tttcgcatgc ttgaacaaga
     1201 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     1261 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     1321 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     1381 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     1441 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     1501 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     1561 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     1621 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     1681 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     1741 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     1801 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     1861 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     1921 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     1981 ataatacgac tcactatagg gtaactaatt gaggcgcgcc taccaccgtc aaaaaaaacg
     2041 gcgcttttta gcgccgtttt tatttttcaa ccttcctagg taagtgaccc tttagtgagg
     2101 gttaatt
//
