LOCUS       pUdO2te_sequence        2656 bp DNA     circular SYN 30-NOV-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2023 from SnapGene Viewer 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     origin          1..848
                     /label=p15A
                     /note="color: #f58a5e"
     misc_feature    36..581
                     /label=minimal p15A ori
                     /note="color: #85dae9"
     primer_bind     849..869
                     /label=M13-rev
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      887..944
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     misc_feature    945..1118
                     /label=MCS1
                     /note="color: #b4abac"
     terminator      1119..1179
                     /label=L3S2P21_ter
                     /note="color: #b1ff67"
     primer_bind     complement(1188..1205)
                     /label=M13-fwd
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    1206..1324
                     /label=TetR_prom
                     /note="color: #84b0dc"
     misc_feature    1325..2530
                     /label=TetR
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     2531..2550
                     /label=T7
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      2570..2623
                     /label=arcA_ter
                     /note="color: #b1ff67"
     promoter        complement(2638..2656)
                     /label=T3 promoter
                     /note="color: #ff9ccd; direction: LEFT"
ORIGIN
        1 ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt
       61 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
      121 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
      181 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
      241 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
      361 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
      421 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
      481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
      541 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc
      601 ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt
      661 aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
      721 cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc
      781 cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca gtatatacac
      841 tccgctagca ggaaacagct atgaccatgc ccgggtaagt gactagcgga aacacagaaa
      901 aaagcccgca cctgacagtg cgggcttttt ttttcgacca aaggctccga agagccgagt
      961 gtcttcggtt cgagaccgaa ttcgcgagct ctcgctagcc agtttaaaca attgcggccg
     1021 cgtactagtc tatcgatttg gatccaccct gcaggaagtc tagaccgtcg acacggtctc
     1081 tgagagaaga cacgtcgctc ttcgacgtaa gtaactgact cggtaccaaa ttccagaaaa
     1141 gaggcctccc gaaagggggg ccttttttcg ttttggtcct aagcttaact ggccgtcgtt
     1201 ttacatacgg ccccaaggtc caaacggtga tagggataac agggtaatgt accatttacg
     1261 ttgacaccac ctttcgcgta tggcatgaca gcgcccggaa gagagtcaat tcagggtggt
     1321 gaatatgaat agttcgacaa agatcgcatt ggtaattacg ttgctcgatg ccatggggat
     1381 tggccttatc atgccagtct tgccaacgtt attacgtgaa tttattgctt cggaagatat
     1441 cgctaaccac tttggcgtat tgcttgcact ttatgcgtta atgcaggtta tctttgctcc
     1501 ttggcttgga aaaatgtctg accgatttgg tcggcgccca gtgctgttgt tgtcattaat
     1561 aggcgcatcg ctggattact tattgctggc tttttcaagt gcgctttgga tgctgtattt
     1621 aggccgtttg ctttcaggga tcacaggagc tactggggct gtcgcggcat cggtcattgc
     1681 cgataccacc tcagcttctc aacgcgtgaa gtggttcggt tggttagggg caagttttgg
     1741 gcttggttta atagcggggc ctattattgg tggttttgca ggagagattt caccgcatag
     1801 tccctttttt atcgctgcgt tgctaaatat tgtcactttc cttgtggtta tgttttggtt
     1861 ccgtgaaacc aaaaatacac gtgataatac agataccgaa gtaggggttg agacgcaatc
     1921 aaattcggtg tacatcactt tatttaaaac gatgcccatt ttgttgatta tttatttttc
     1981 agcgcaattg ataggccaaa ttcccgcaac ggtgtgggtg ctatttaccg aaaatcgttt
     2041 tggatggaat agcatgatgg ttggcttttc attagcgggt cttggtcttt tacactcagt
     2101 attccaagcc tttgtggcag gaagaatagc cactaaatgg ggcgaaaaaa cggcagtact
     2161 gctcggattt attgcagata gtagtgcatt tgccttttta gcgtttatat ctgaaggttg
     2221 gttagttttc cctgttttaa ttttattggc tggtggtggg atcgctttac ctgcattaca
     2281 gggagtgatg tctatccaaa caaagagtca tcagcaaggt gctttacagg gattattggt
     2341 gagccttacc aatgcaaccg gtgttattgg cccattactg tttgctgtta tttataatca
     2401 ttcactacca atttgggatg gctggatttg gattattggt ttagcgtttt actgtattat
     2461 tatcctgcta tcaatgacct tcatgttgac ccctcaagct caggggagta aacaggagac
     2521 aagtgcttag taatacgact cactataggg taactaattg aggcgcgcct accaccgtca
     2581 aaaaaaacgg cgctttttag cgccgttttt atttttcaac cttcctaggt aagtgaccct
     2641 ttagtgaggg ttaatt
//
