LOCUS       pUdO4t_sequenced        2221 bp DNA     circular SYN 30-NOV-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 30, 2023 from SnapGene Viewer 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     origin          1..1433
                     /label=pBBR1
                     /note="color: #f58a5e"
     misc_feature    1..770
                     /label=oriV
                     /note="color: #85dae9"
     misc_feature    771..1433
                     /label=Rep
                     /note="color: #85dae9"
     primer_bind     1434..1454
                     /label=M13-rev
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      1472..1529
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     misc_feature    1530..1703
                     /label=MCS1
                     /note="color: #b4abac"
     terminator      1704..1764
                     /label=L3S2P21_ter
                     /note="color: #b1ff67"
     primer_bind     complement(1773..1790)
                     /label=M13-fwd
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    1791..1858
                     /label=TmpR_prom
                     /note="color: #84b0dc"
     CDS             1859..2095
                     /label=TmpR
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     2096..2115
                     /label=T7
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      2135..2188
                     /label=arcA_ter
                     /note="color: #b1ff67"
     promoter        complement(2203..2221)
                     /label=T3 promoter
                     /note="color: #ff9ccd; direction: LEFT"
ORIGIN
        1 gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg
       61 gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt
      121 cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt
      181 aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac
      241 accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc
      301 gcctggaacg acccaagcct atgcgagtgg gggcagtcga aggcgaagcc cgcccgcctg
      361 ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg gggcagcgcc accttgggca
      421 aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg gccacttttt gccggagggg
      481 gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc cttctttggg caccaaagaa
      541 ctagatatag ggcgaaatgc gaaagactta aaaatcaaca acttaaaaaa ggggggtacg
      601 caacagctca ttgcggcacc ccccgcaata gctcattgcg taggttaaag aaaatctgta
      661 attgactgcc acttttacgc aacgcataat tgttgtcgcg ctgccgaaaa gttgcagctg
      721 attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat ggagataagc atggccacgc
      781 agtccagaga aatcggcatt caagccaaga acaagcccgg tcactgggtg caaacggaac
      841 gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa acccacggcg gcaatgctgc
      901 tgcatcacct cgtggcgcag atgggccacc agaacgccgt ggtggtcagc cagaagacac
      961 tttccaagct catcggacgt tctttgcgga cggtccaata cgcagtcaag gacttggtgg
     1021 ccgagcgctg gatctccgtc gtgaagctca acggccccgg caccgtgtcg gcctacgtgg
     1081 tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt gcgcctgtcg gtgttcagtg
     1141 ccgccgtggt ggttgatcac gacgaccagg acgaatcgct gttggggcat ggcgacctgc
     1201 gccgcatccc gaccctgtat ccgggcgagc agcaactacc gaccggcccc ggcgaggagc
     1261 cgcccagcca gcccggcatt ccgggcatgg aaccagacct gccagccttg accgaaacgg
     1321 aggaatggga acggcgcggg cagcagcgcc tgccgatgcc cgatgagccg tgttttctgg
     1381 acgatggcga gccgttggag ccgccgacac gggtcacgct gccgcgccgg tagcaggaaa
     1441 cagctatgac catgcccggg taagtgacta gcggaaacac agaaaaaagc ccgcacctga
     1501 cagtgcgggc tttttttttc gaccaaaggc tccgaagagc cgagtgtctt cggttcgaga
     1561 ccgaattcgc gagctctcgc tagccagttt aaacaattgc ggccgcgtac tagtctatcg
     1621 atttggatcc accctgcagg aagtctagac cgtcgacacg gtctctgaga gaagacacgt
     1681 cgctcttcga cgtaagtaac tgactcggta ccaaattcca gaaaagaggc ctcccgaaag
     1741 gggggccttt tttcgttttg gtcctaagct taactggccg tcgttttaca ctgttgacaa
     1801 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     1861 gggtcaaagt agcgatgaag ccaacgctcc cgttgcaggg cagtttgcgc ttcccctgag
     1921 tgccaccttt ggcttagggg atcgcgtacg caagaaatct ggtgccgctt ggcagggtca
     1981 agtcgtcggt tggtattgca caaaactcac tcctgaaggc tatgcggtcg agtccgaatc
     2041 ccacccaggc tcagtgcaaa tttatcctgt ggctgcactt gaacgtgtgg cctaataata
     2101 cgactcacta tagggtaact aattgaggcg cgcctaccac cgtcaaaaaa aacggcgctt
     2161 tttagcgccg tttttatttt tcaaccttcc taggtaagtg accctttagt gagggttaat
     2221 t
//
