LOCUS       pTSAR3.5t        3923 bp DNA     circular SYN 03-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     origin          1..629
                     /label=colE1
                     /note="color: #f58a5e"
     primer_bind     630..650
                     /label=M13-rev
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      668..725
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     promoter        739..817
                     /label=rpsM promoter
                     /note="color: #85dae9"
     misc_feature    873..1592
                     /label=eBFP2
                     /note="color: #b7e6d7"
     terminator      1606..1658
                     /label=L3S3P21_ter
                     /note="color: #ffef86"
     misc_feature    1665..1980
                     /label=ProminIH7_8
                     /note="color: #faac61"
     misc_feature    1993..2015
                     /label=SD+transI_Init_U
                     /note="color: #f8d3a9"
     misc_feature    2016..2726
                     /label=GFP_SFM2[5'][*]
                     /note="color: #b1ff67"
     misc_feature    2727..2765
                     /label=ssrA_d2
                     /note="color: #c7b0e3"
     terminator      2780..2840
                     /label=L3S2P21_ter
                     /note="color: #b1ff67"
     primer_bind     complement(2849..2866)
                     /label=M13-fwd
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    2867..2895
                     /label=AmpR promoter
                     /note="color: #c7b0e3"
     CDS             2937..3794
                     /label=AmpR
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     3798..3817
                     /label=T7
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      3837..3890
                     /label=arcA_ter
                     /note="color: #b1ff67"
     promoter        complement(3905..3923)
                     /label=T3 promoter
                     /note="color: #ff9ccd; direction: LEFT"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctttgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggccc aggaaacagc tatgaccatg cccgggtaag
      661 tgactagcgg aaacacagaa aaaagcccgc acctgacagt gcgggctttt tttttcgacc
      721 aaaggctccg gcggccgcga ttttttcgca tatttttctt gcaaagttgg gttgagctgg
      781 ctagattagc cagccaatct tttgtatgtc tgtgcgttcg agtaactagc taagattacg
      841 aattcgagct cggtaccggg taaggaggta aaatggtgag caagggcgag gagctgttca
      901 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      961 tgaggggcga gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca
     1021 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgagc cacggcgtgc
     1081 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1141 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcacc tacaagaccc
     1201 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcgtcg
     1261 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccaca
     1321 acatctatat catggccgtc aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     1381 acaacgtgga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1441 gcgacggccc cgtgctgctg cccgacagcc actacctgag cacccagtcc gtgctgagca
     1501 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttccgcacc gccgccggga
     1561 tcactctcgg catggacgag ctgtacaagt aaccatggag atatcccaat tattgaaggc
     1621 ctccctaacg gggggccttt ttttgtttct ggtctcccgc tagcgaattg ccctcgtatt
     1681 aaatgtgtat cgttttttta cagtaatttt aatttgttat tctataatag gaataaatga
     1741 gcttgactaa aatatgatgt aaaacaaata tacttactcg gattacattt ccttgctcac
     1801 tggaatgttg aattcagtgt tattattgac tgcagtcaca ataaaaaaga attccataga
     1861 aaacctccat aaataaatta caactaactt ctgttatgtg taaaatggaa actattaaaa
     1921 cttaatatcg gaaatggtaa gtgaaatttg tataaatata caattttaaa tatttattct
     1981 aacgttctag ctaggagaaa ttaaccatga aaagatctaa aggagaagaa cttttcactg
     2041 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtcc
     2101 gtggagaggg tgaaggtgat gctacaaacg gaaaactcac ccttaaattt atttgcacta
     2161 ctggaaaact acctgttccg tggccaacac ttgtcactac ttttgcgtat ggcctgcagt
     2221 gctttgcacg ttatccggat cacatgaaac ggcatgactt tttcaagagt gccatgcccg
     2281 aaggttatgt acaggaacgc actatatctt tcaaagatga cgggacctac aagacgcgtg
     2341 ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat cgagttaaag ggtattgatt
     2401 ttaaagaaga tggaaacatt cttggacaca aactcgagta caactttaac tcacacaatg
     2461 tatacatcac ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa attcgccaca
     2521 acgttgaaga tggttccgtt caactagcag accattatca acaaaatact ccaattggcg
     2581 atggccctgt ccttttacca gacaaccatt acctgtcgac acaatctgtc ctttcgaaag
     2641 atcccaacga aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct gctgggatta
     2701 cacatggcat ggatgaacta tacaaacggc cggcggccaa cgatgaaaat tatgcgctgg
     2761 ccgcataatc tagaactgac tcggtaccaa attccagaaa agaggcctcc cgaaaggggg
     2821 gccttttttc gttttggtcc taagcttaac tggccgtcgt tttacattca aatatgtatc
     2881 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2941 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3001 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3061 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3121 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     3181 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3241 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3301 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3361 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3421 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     3481 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3541 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3601 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3661 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3721 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3781 tgattaagca ttggtaataa tacgactcac tatagggtaa ctaattgagg cgcgcctacc
     3841 accgtcaaaa aaaacggcgc tttttagcgc cgtttttatt tttcaacctt cctaggtaag
     3901 tgacccttta gtgagggtta att
//
