LOCUS       pudom1.1                2852 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2852
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          825..1049
                     /label=source
                     /note="color: #d6b295"
     terminator      9..66
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     misc_feature    67..650
                     /label=CMV promoter (584 bp)
                     /label=CMVb (584bp)
                     /note="color: #d59687"
     misc_feature    651..824
                     /label=MCS1
                     /note="color: #ff9900"
     polyA_signal    825..1049
                     /label=bGH poly(A) signal
                     /note="color: #d59687"
     primer_bind     complement(825..836)
                     /label=BGH-rev
                     /note="color: #b1ff67; direction: LEFT"
     terminator      1050..1110
                     /label=L3S2P21_ter
                     /note="color: #ff9ccd"
     primer_bind     complement(1119..1136)
                     /label=M13-fwd
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1137..1165
                     /label=AmpR promoter
                     /note="color: #b1ff67"
     CDS             1207..2064
                     /label=AmpR
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     2068..2087
                     /label=T7
                     /note="color: #75c6a9; direction: RIGHT"
     terminator      2107..2160
                     /label=arcA_ter
                     /note="color: #ffef86"
     promoter        complement(2175..2193)
                     /label=T3 promoter
                     /note="color: #9eafd2; direction: LEFT"
     misc_feature    2194..2822
                     /label=colE1
                     /note="color: #00ccff"
     primer_bind     2823..2843
                     /label=M13-rev
                     /note="color: #85dae9; direction: RIGHT"
ORIGIN
        1 gtgactagcg gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac
       61 caaagggaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      121 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      181 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      241 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      301 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      361 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      421 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      481 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      541 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      601 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccgaagag
      661 ccgagtgtct tcggttcgag accgaattcg cgagctctcg ctagccagtt taaacaattg
      721 cggccgcgta ctagtctatc gatttggatc caccctgcag gaagtctaga ccgtcgacac
      781 ggtctctgag agaagacacg tcgctcttcg acgtaagtaa ctgactgtgc cttctagttg
      841 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
      901 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
      961 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     1021 gcatgctggg gatgcggtgg gctctatggc tcggtaccaa attccagaaa agaggcctcc
     1081 cgaaaggggg gccttttttc gttttggtcc taagcttaac tggccgtcgt tttacattca
     1141 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1201 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1261 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     1321 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     1381 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1441 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1501 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1561 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1621 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1681 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1741 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1801 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1861 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     1921 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1981 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2041 ggtgcctcac tgattaagca ttggtaataa tacgactcac tatagggtaa ctaattgagg
     2101 cgcgcctacc accgtcaaaa aaaacggcgc tttttagcgc cgtttttatt tttcaacctt
     2161 cctaggtaag tgacccttta gtgagggtta atttagaaaa gatcaaagga tcttcttgag
     2221 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2281 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2341 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2401 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2461 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2521 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2581 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2641 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2701 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2761 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2821 cccaggaaac agctatgacc atgcccgggt aa
//
