LOCUS       pudom1.2                2776 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2776
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          749..973
                     /label=source
                     /note="color: #d6b295"
     terminator      9..66
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     misc_feature    67..574
                     /label=CMV promoter (508 bp)
                     /label=CMVshort(508bp)
                     /note="color: #9eafd2"
     misc_feature    575..748
                     /label=MCS1
                     /note="color: #ff9900"
     polyA_signal    749..973
                     /label=bGH poly(A) signal
                     /note="color: #d59687"
     primer_bind     complement(749..760)
                     /label=BGH-rev
                     /note="color: #b1ff67; direction: LEFT"
     terminator      974..1034
                     /label=L3S2P21_ter
                     /note="color: #ff9ccd"
     primer_bind     complement(1043..1060)
                     /label=M13-fwd
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1061..1089
                     /label=AmpR promoter
                     /note="color: #b1ff67"
     CDS             1131..1988
                     /label=AmpR
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     1992..2011
                     /label=T7
                     /note="color: #75c6a9; direction: RIGHT"
     terminator      2031..2084
                     /label=arcA_ter
                     /note="color: #ffef86"
     promoter        complement(2099..2117)
                     /label=T3 promoter
                     /note="color: #9eafd2; direction: LEFT"
     misc_feature    2118..2746
                     /label=colE1
                     /note="color: #00ccff"
     primer_bind     2747..2767
                     /label=M13-rev
                     /note="color: #85dae9; direction: RIGHT"
ORIGIN
        1 gtgactagcg gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac
       61 caaaggcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctctccga agagccgagt gtcttcggtt
      601 cgagaccgaa ttcgcgagct ctcgctagcc agtttaaaca attgcggccg cgtactagtc
      661 tatcgatttg gatccaccct gcaggaagtc tagaccgtcg acacggtctc tgagagaaga
      721 cacgtcgctc ttcgacgtaa gtaactgact gtgccttcta gttgccagcc atctgttgtt
      781 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      841 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
      901 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
      961 gtgggctcta tggctcggta ccaaattcca gaaaagaggc ctcccgaaag gggggccttt
     1021 tttcgttttg gtcctaagct taactggccg tcgttttaca ttcaaatatg tatccgctca
     1081 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1141 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1201 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1261 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1321 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1381 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1441 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1501 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1561 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1621 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1681 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1741 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1801 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1861 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1921 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1981 agcattggta ataatacgac tcactatagg gtaactaatt gaggcgcgcc taccaccgtc
     2041 aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa ccttcctagg taagtgaccc
     2101 tttagtgagg gttaatttag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2281 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcccagg aaacagctat
     2761 gaccatgccc gggtaa
//
