LOCUS       pudom1.4                2320 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2320
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     source          293..517
                     /label=source
                     /note="color: #d6b295"
     terminator      9..66
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     promoter        67..118
                     /label=CMV promoter (52 bp)
                     /label=CMV promoter
                     /note="color: #c6c9d1"
     misc_feature    119..292
                     /label=MCS1
                     /note="color: #ff9900"
     polyA_signal    293..517
                     /label=bGH poly(A) signal
                     /note="color: #d59687"
     primer_bind     complement(293..304)
                     /label=BGH-rev
                     /note="color: #b1ff67; direction: LEFT"
     terminator      518..578
                     /label=L3S2P21_ter
                     /note="color: #ff9ccd"
     primer_bind     complement(587..604)
                     /label=M13-fwd
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    605..633
                     /label=AmpR promoter
                     /note="color: #b1ff67"
     CDS             675..1532
                     /label=AmpR
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     1536..1555
                     /label=T7
                     /note="color: #75c6a9; direction: RIGHT"
     terminator      1575..1628
                     /label=arcA_ter
                     /note="color: #ffef86"
     promoter        complement(1643..1661)
                     /label=T3 promoter
                     /note="color: #9eafd2; direction: LEFT"
     misc_feature    1662..2290
                     /label=colE1
                     /note="color: #00ccff"
     primer_bind     2291..2311
                     /label=M13-rev
                     /note="color: #85dae9; direction: RIGHT"
ORIGIN
        1 gtgactagcg gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac
       61 caaagggacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
      121 ccgaagagcc gagtgtcttc ggttcgagac cgaattcgcg agctctcgct agccagttta
      181 aacaattgcg gccgcgtact agtctatcga tttggatcca ccctgcagga agtctagacc
      241 gtcgacacgg tctctgagag aagacacgtc gctcttcgac gtaagtaact gactgtgcct
      301 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
      361 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
      421 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
      481 aatagcaggc atgctgggga tgcggtgggc tctatggctc ggtaccaaat tccagaaaag
      541 aggcctcccg aaaggggggc cttttttcgt tttggtccta agcttaactg gccgtcgttt
      601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      661 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      721 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      781 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      841 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      901 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
      961 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1021 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1081 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1141 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1201 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1261 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1321 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1381 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1441 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1501 ctgagatagg tgcctcactg attaagcatt ggtaataata cgactcacta tagggtaact
     1561 aattgaggcg cgcctaccac cgtcaaaaaa aacggcgctt tttagcgccg tttttatttt
     1621 tcaaccttcc taggtaagtg accctttagt gagggttaat ttagaaaaga tcaaaggatc
     1681 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1741 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1801 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1861 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1921 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1981 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2041 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2101 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2161 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2221 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2281 caacgcggcc caggaaacag ctatgaccat gcccgggtaa
//
