LOCUS       pudom4-1                2586 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      10..67
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     misc_feature    68..651
                     /label=CMV promoter (584 bp)
                     /note="color: #99cc00"
     misc_feature    652..825
                     /label=MCS1
                     /note="color: #ff9900"
     polyA_signal    826..960
                     /label=SV40 poly(A) signal
                     /note="color: #b4abac"
     terminator      961..1021
                     /label=L3S2P21_ter
                     /note="color: #ff9ccd"
     primer_bind     complement(1030..1047)
                     /label=M13-fwd
                     /note="color: #ff9ccd; direction: LEFT"
     CDS             1143..1799
                     /label=CmR
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     1803..1822
                     /label=T7
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      1842..1895
                     /label=arcA_ter
                     /note="color: #b1ff67"
     promoter        complement(1910..1928)
                     /label=T3 promoter
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    1929..2557
                     /label=colE1
                     /note="color: #00ccff"
     primer_bind     2558..2578
                     /label=M13-rev
                     /note="color: #ff9ccd; direction: RIGHT"
ORIGIN
        1 agtgactagc ggaaacacag aaaaaagccc gcacctgaca gtgcgggctt tttttttcga
       61 ccaaagggac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      121 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      181 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      241 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      301 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      361 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      421 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      481 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      541 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      601 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccgaaga
      661 gccgagtgtc ttcggttcga gaccgaattc gcgagctctc gctagccagt ttaaacaatt
      721 gcggccgcgt actagtctat cgatttggat ccaccctgca ggaagtctag accgtcgaca
      781 cggtctctga gagaagacac gtcgctcttc gacgtaagta actgaaactt gtttattgca
      841 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      901 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
      961 ctcggtacca aattccagaa aagaggcctc ccgaaagggg ggcctttttt cgttttggtc
     1021 ctaagcttaa ctggccgtcg ttttacaacg taagaggttc caactttcac cataatgaaa
     1081 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta
     1141 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     1201 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     1261 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     1321 ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg
     1381 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     1441 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     1501 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     1561 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     1621 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     1681 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     1741 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     1801 aataatacga ctcactatag ggtaactaat tgaggcgcgc ctaccaccgt caaaaaaaac
     1861 ggcgcttttt agcgccgttt ttatttttca accttcctag gtaagtgacc ctttagtgag
     1921 ggttaattta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2341 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2521 ggggcggagc ctatggaaaa acgccagcaa cgcggcccag gaaacagcta tgaccatgcc
     2581 cgggta
//
