LOCUS       pudom5-1                2226 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          828..1052
                     /label=source
                     /note="color: #d6b295"
     terminator      12..69
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     misc_feature    70..653
                     /label=CMV promoter (584 bp)
                     /note="color: #99cc00"
     misc_feature    654..827
                     /label=MCS1
                     /note="color: #ff9900"
     polyA_signal    828..1052
                     /label=bGH poly(A) signal
                     /note="color: #d59687"
     primer_bind     complement(828..839)
                     /label=BGH-rev
                     /note="color: #b1ff67; direction: LEFT"
     terminator      1053..1113
                     /label=L3S2P21_ter
                     /note="color: #ff9ccd"
     primer_bind     complement(1122..1139)
                     /label=M13-fwd
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    1140..1444
                     /label=TpR
                     /note="color: #ffef86"
     primer_bind     1445..1464
                     /label=T7
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      1484..1537
                     /label=arcA_ter
                     /note="color: #b1ff67"
     promoter        complement(1552..1570)
                     /label=T3 promoter
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    1571..2199
                     /label=colE1
                     /note="color: #00ccff"
     primer_bind     2200..2220
                     /label=M13-rev
                     /note="color: #ff9ccd; direction: RIGHT"
ORIGIN
        1 taagtgacta gcggaaacac agaaaaaagc ccgcacctga cagtgcgggc tttttttttc
       61 gaccaaaggg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      121 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      181 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      241 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      301 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      361 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      421 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      481 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      541 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      601 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctccgaa
      661 gagccgagtg tcttcggttc gagaccgaat tcgcgagctc tcgctagcca gtttaaacaa
      721 ttgcggccgc gtactagtct atcgatttgg atccaccctg caggaagtct agaccgtcga
      781 cacggtctct gagagaagac acgtcgctct tcgacgtaag taactgactg tgccttctag
      841 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      901 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      961 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     1021 caggcatgct ggggatgcgg tgggctctat ggctcggtac caaattccag aaaagaggcc
     1081 tcccgaaagg ggggcctttt ttcgttttgg tcctaagctt aactggccgt cgttttacac
     1141 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     1201 ctaaaccatg ggtcaaagta gcgatgaagc caacgctccc gttgcagggc agtttgcgct
     1261 tcccctgagt gccacctttg gcttagggga tcgcgtacgc aagaaatctg gtgccgcttg
     1321 gcagggtcaa gtcgtcggtt ggtattgcac aaaactcact cctgaaggct atgcggtcga
     1381 gtccgaatcc cacccaggct cagtgcaaat ttatcctgtg gctgcacttg aacgtgtggc
     1441 ctagtaatac gactcactat agggtaacta attgaggcgc gcctaccacc gtcaaaaaaa
     1501 acggcgcttt ttagcgccgt ttttattttt caaccttcct aggtaagtga ccctttagtg
     1561 agggttaatt tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1621 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1681 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1741 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1801 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1861 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1921 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1981 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2041 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2101 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2161 ggggggcgga gcctatggaa aaacgccagc aacgcggccc aggaaacagc tatgaccatg
     2221 cccggg
//
