LOCUS       pudom7-1                2364 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          825..1049
                     /label=source
                     /note="color: #d6b295"
     terminator      9..66
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     misc_feature    67..650
                     /label=CMV promoter (584 bp)
                     /note="color: #99cc00"
     misc_feature    651..824
                     /label=MCS1
                     /note="color: #ff9900"
     polyA_signal    825..1049
                     /label=bGH poly(A) signal
                     /note="color: #d59687"
     primer_bind     complement(825..836)
                     /label=BGH-rev
                     /note="color: #b1ff67; direction: LEFT"
     terminator      1050..1110
                     /label=L3S2P21_ter
                     /note="color: #ff9ccd"
     primer_bind     complement(1119..1136)
                     /label=M13-fwd
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    1137..1579
                     /label=ZeoR
                     /note="color: #ffff99"
     primer_bind     1580..1599
                     /label=T7
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      1619..1672
                     /label=arcA_ter
                     /note="color: #ffef86"
     promoter        complement(1687..1705)
                     /label=T3 promoter
                     /note="color: #9eafd2; direction: LEFT"
     misc_feature    1706..2334
                     /label=colE1
                     /note="color: #00ccff"
     primer_bind     2335..2355
                     /label=M13-rev
                     /note="color: #85dae9; direction: RIGHT"
ORIGIN
        1 gtgactagcg gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac
       61 caaagggaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      121 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      181 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      241 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      301 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      361 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      421 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      481 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      541 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      601 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccgaagag
      661 ccgagtgtct tcggttcgag accgaattcg cgagctctcg ctagccagtt taaacaattg
      721 cggccgcgta ctagtctatc gatttggatc caccctgcag gaagtctaga ccgtcgacac
      781 ggtctctgag agaagacacg tcgctcttcg acgtaagtaa ctgactgtgc cttctagttg
      841 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
      901 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
      961 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     1021 gcatgctggg gatgcggtgg gctctatggc tcggtaccaa attccagaaa agaggcctcc
     1081 cgaaaggggg gccttttttc gttttggtcc taagcttaac tggccgtcgt tttacagtgt
     1141 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     1201 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     1261 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     1321 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     1381 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     1441 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     1501 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     1561 ggccgaggag caggactgat aatacgactc actatagggt aactaattga ggcgcgccta
     1621 ccaccgtcaa aaaaaacggc gctttttagc gccgttttta tttttcaacc ttcctaggta
     1681 agtgaccctt tagtgagggt taatttagaa aagatcaaag gatcttcttg agatcctttt
     1741 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1801 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1861 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1921 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1981 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2041 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2101 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2161 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2221 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2281 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggcccaggaa
     2341 acagctatga ccatgcccgg gtaa
//
