LOCUS       pudom8-1                2274 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 5, 2023 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      7..64
                     /label=thrLABC_ter
                     /note="color: #b1ff67"
     misc_feature    65..648
                     /label=CMV promoter (584 bp)
                     /note="color: #a6acb3"
     misc_feature    649..822
                     /label=MCS1
                     /note="color: #ff9900"
     polyA_signal    823..957
                     /label=SV40 poly(A) signal
                     /note="color: #b4abac"
     terminator      958..1018
                     /label=L3S2P21_ter
                     /note="color: #ff9ccd"
     primer_bind     complement(1027..1044)
                     /label=M13-fwd
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    1045..1487
                     /label=ZeoR
                     /note="color: #ffff99"
     primer_bind     1488..1507
                     /label=T7
                     /note="color: #ff9ccd; direction: RIGHT"
     terminator      1527..1580
                     /label=arcA_ter
                     /note="color: #ffef86"
     promoter        complement(1595..1613)
                     /label=T3 promoter
                     /note="color: #9eafd2; direction: LEFT"
     misc_feature    1614..2242
                     /label=colE1
                     /note="color: #00ccff"
     primer_bind     2243..2263
                     /label=M13-rev
                     /note="color: #85dae9; direction: RIGHT"
ORIGIN
        1 gactagcgga aacacagaaa aaagcccgca cctgacagtg cgggcttttt ttttcgacca
       61 aagggacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      121 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      181 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      241 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      301 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      361 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      421 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      481 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      541 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      601 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ccgaagagcc
      661 gagtgtcttc ggttcgagac cgaattcgcg agctctcgct agccagttta aacaattgcg
      721 gccgcgtact agtctatcga tttggatcca ccctgcagga agtctagacc gtcgacacgg
      781 tctctgagag aagacacgtc gctcttcgac gtaagtaact gaaacttgtt tattgcagct
      841 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      901 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcctc
      961 ggtaccaaat tccagaaaag aggcctcccg aaaggggggc cttttttcgt tttggtccta
     1021 agcttaactg gccgtcgttt tacagtgttg acaattaatc atcggcatag tatatcggca
     1081 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     1141 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     1201 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     1261 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     1321 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     1381 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     1441 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgataa tacgactcac
     1501 tatagggtaa ctaattgagg cgcgcctacc accgtcaaaa aaaacggcgc tttttagcgc
     1561 cgtttttatt tttcaacctt cctaggtaag tgacccttta gtgagggtta atttagaaaa
     1621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1741 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2221 gaaaaacgcc agcaacgcgg cccaggaaac agctatgacc atgcccgggt aagt
//
