LOCUS       Exported               10250 bp ds-DNA     circular SYN 09-OCT-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 10, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     [DEFINITION]: .
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3072..3090
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     812..831
                     /label=BII(R)
                     /note="""/label=BII(R) - /ApEinfo_fwdcolor=#5381ff - 
                     /ApEinfo_revcolor=#19ff25 - 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     /width 5 offset 0 -"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggccttc gacattgatt attgactagt tattaatagt
     1381 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1441 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1501 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt
     1561 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1621 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     1681 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ggtcgaggtg
     1741 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
     1801 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc
     1861 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
     1921 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     1981 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg ttgccttcgc cccgtgcccc
     2041 gctccgcgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     2101 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     2161 cgtttctttt ctgtggctgc gtgaaagcct taaagggctc cgggagggcc ctttgtgcgg
     2221 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc
     2281 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcgt
     2341 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggc tgcgagggga
     2401 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg
     2461 tcgggctgta acccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
     2521 ggtgcggggc tccgtgcggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
     2581 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg
     2641 cgcggcggcc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     2701 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgg cggagccgaa
     2761 atctgggagg cgccgccgca ccccctctag cgggcgcggg cgaagcggtg cggcgccggc
     2821 aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat
     2881 ctccagcctc ggggctgccg cagggggacg gctgccttcg ggggggacgg ggcagggcgg
     2941 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     3001 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     3061 gcaaaggcta gtaatacgac tcactatagg gctagcgcca ccatggtgag caagggcgag
     3121 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     3181 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     3241 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     3301 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     3361 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     3421 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     3481 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     3541 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     3601 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     3661 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     3721 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     3781 tccaccggcg gcatggacga gctgtacaag ggatccggcg caacaaactt ctctctgctg
     3841 aaacaagccg gagatgtcga agagaatcct ggaccgatgt tggggtttgt gggtcgggtg
     3901 gccgctgctc cggcctccgg ggccttgcgg agactcaccc cttcagcgtc gctgccccca
     3961 gctcagctct tactgcgggc cgctccgacg gcggtccatc ctgtcaggga ctatgcggcg
     4021 caaacatctg agagcggcgg aggcgggagc cccggcgcgg ccgcggacta caaggatgac
     4081 gacgataaag gtagcgtgga tctacgcacg ctcggctaca gccagcagca acaggagaag
     4141 atcaaaccga aggttcgttc gacagtggcg cagcaccacg aggcactggt cggccatggg
     4201 tttacacacg cgcacatcgt tgcgctcagc caacacccgg cagcgttagg gaccgtcgct
     4261 gtcaagtatc aggacatgat cgcagcgttg ccagaggcga cacacgaagc gatcgttggc
     4321 gtcggcaaac agtggtccgg cgcacgcgct ctggaggcct tgctcacggt ggcgggagag
     4381 ttgagaggtc caccgttaca gttggacaca ggccaacttc tcaagattgc aaaacgtggc
     4441 ggcgtgaccg cagtggaggc agtgcatgca tggcgcaatg cactgacggg tgcccccctg
     4501 aacctgaccc cggagcaggt ggtggccatc gctagtaata ttggtggcaa acaggctctt
     4561 gagacggttc agcgcctcct tccagttctc tgtcaagccc acggactcac cccagatcaa
     4621 gttgtagcga ttgctagtaa tattggtggc aaacaggctc ttgagacggt tcagcgcctc
     4681 cttccagttc tctgtcaagc ccacggactc accccagatc aggttgtagc gattgctagt
     4741 aatattggtg gcaaacaggc tcttgagacg gttcagcgcc tccttccagt tctctgtcaa
     4801 gcccacggac tcaccccaga tcaagttgta gcgattgcta gtaatattgg tggcaaacag
     4861 gctcttgaga cggttcagcg cctccttcca gttctctgtc aagcccacgg actcacccca
     4921 gatcaagtgg tagcgattgc tagtaacaat ggtggcaaac aggctcttga aaccgtacag
     4981 cgcctactgc cagttctctg tcaagcccac ggtctgactc cggagcaagt tgtagcgatt
     5041 gctagtaata ttggtggcaa acaggctctt gagacggttc agcgcctcct tccagttctc
     5101 tgtcaagccc acggactcac cccagatcaa gttgtagcga ttgctagtaa caatggtggc
     5161 aaacaggctc ttgaaaccgt acagcgccta ctgccagttc tctgtcaagc ccacggtctg
     5221 actccggagc aagttgtagc gattgctagt aatgggggtg gcaaacaggc tcttgagact
     5281 gttcagcgcc ttctaccagt tctctgtcaa gcccacggcc tgacgcccga gcaagttgta
     5341 gcaattgcta gtaatggggg tggcaaacag gctcttgaga ctgttcagcg ccttctacca
     5401 gttctctgtc aagcccacgg cctgacgccc gagcaagttg tagcgattgc tagtaatatt
     5461 ggtggcaaac aggctcttga gacggttcag cgcctccttc cagttctctg tcaagcccac
     5521 ggactcaccc cagatcaagt tgtagcgatt gctagtcatg acggtggcaa acaggctctt
     5581 gaaaccgtcc aacgccttct accagttctc tgtcaagccc acggactaac cccagcgcaa
     5641 gttgtagcga ttgctagtaa tgggggtggc aaacaggctc ttgagactgt tcagcgcctt
     5701 ctaccagttc tctgtcaagc ccacggcctg acgcccgagc aagttgtagc gatagccagt
     5761 aatgggggtg gcaaacaggc tcttgagact gttcagcgcc ttctaccagt tctctgtcaa
     5821 gcccacggcc tgacgcccga gcaagttgta gcgattgcta gtaatggggg tggcaaacag
     5881 gctcttgaga ctgttcagcg ccttctacca gttctctgtc aagcccacgg cctgacgccc
     5941 gagcaagttg tagcgattgc aagtaacaat ggtggcaaac aggctcttga aaccgtacag
     6001 cgcctactgc cagttctctg tcaagcccac ggtctgactc cggagcaagt tgtagcgatt
     6061 gctagtaata ttggtggcaa acaggctctt gagacggttc agcgcctcct tccagttctc
     6121 tgtcaagccc acggactcac cccagatcaa gttgtagcga ttgcttccaa tgggggcggc
     6181 aggccggcgc tggagagcat tgttgcccag ttatctcgcc ctgatccggc gttggccgcg
     6241 ttgaccaacg accacctcgt cgccttggcc tgcctcggcg gacgtcctgc gctggatgca
     6301 gtgaaaaagg gattgggagg ctccgcaatc cctgtgaaga gaggcgccac cggcgagaca
     6361 aaggtgttta ccggcaactc taatagccct aagtccccaa caaagggcgg ctgctctgga
     6421 ggcagcacca acctgtctga catcatcgag aaggagacag gcaagcagct ggtcatccag
     6481 gagagcatcc tgatgctgcc cgaagaagtc gaagaagtga tcggaaacaa gcctgagagc
     6541 gatatcctgg tccataccgc ctacgacgag agtaccgacg aaaatgtgat gctgctgaca
     6601 tccgacgccc cagagtataa gccctgggct ctggtcatcc aggattccaa cggagagaac
     6661 aaaatcaaaa tgctgtaatc tagagggccc gtttaaacgt cgacaatcaa cctctggatt
     6721 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6781 gatacgctgc tttaatgcct ttgtatcatg cgttaactaa acttgtttat tgcagcttat
     6841 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6901 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gaattgactc
     6961 aaatgatgtc aattagtcta tcagaagctc atctggtctc ccttccgggg gacaagacat
     7021 ccctgtttaa tatttaaaca gcagtgttcc caaactgggt tcttatatcc cttgctctgg
     7081 tcaaccaggt tgcagggttt cctgtcctca caggaacgaa gtccctaaag aaacagtggc
     7141 agccaggttt agccccggaa ttgactggat tcctttttta gggcccattg gtatggcttt
     7201 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
     7261 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
     7321 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
     7381 gggagggacg taattacatc cctgggggct ttgggggggg gctgtccctg atatctataa
     7441 caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt
     7501 atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac
     7561 gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga
     7621 gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa
     7681 gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt
     7741 aatctagctg catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag
     7801 ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc
     7861 ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac
     7921 gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt
     7981 tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag
     8041 cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt
     8101 gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg
     8161 ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg
     8221 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8281 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8341 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8401 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8461 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8521 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8581 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8641 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8701 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8761 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8821 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8881 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8941 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9001 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9061 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9121 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9181 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9241 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9301 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9361 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9421 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9481 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9541 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9601 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9661 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9721 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9781 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9841 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9901 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9961 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10021 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10081 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10141 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10201 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
//
