LOCUS       Exported               10289 bp ds-DNA     circular SYN 09-OCT-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 10, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     [DEFINITION]: .
FEATURES             Location/Qualifiers
     source          1..10289
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3072..3090
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3103..3819
                     /organism="pEGFP-N1"
                     /mol_type="other DNA"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     812..831
                     /label=BII(R)
                     /note="""/label=BII(R) - /ApEinfo_fwdcolor=#5381ff - 
                     /ApEinfo_revcolor=#19ff25 - 
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} - 
                     /width 5 offset 0 -"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggccttc gacattgatt attgactagt tattaatagt
     1381 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1441 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1501 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggactatt
     1561 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1621 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     1681 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ggtcgaggtg
     1741 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
     1801 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc
     1861 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
     1921 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     1981 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg ttgccttcgc cccgtgcccc
     2041 gctccgcgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     2101 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     2161 cgtttctttt ctgtggctgc gtgaaagcct taaagggctc cgggagggcc ctttgtgcgg
     2221 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc
     2281 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcgt
     2341 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggc tgcgagggga
     2401 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg
     2461 tcgggctgta acccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
     2521 ggtgcggggc tccgtgcggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
     2581 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg
     2641 cgcggcggcc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     2701 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgg cggagccgaa
     2761 atctgggagg cgccgccgca ccccctctag cgggcgcggg cgaagcggtg cggcgccggc
     2821 aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat
     2881 ctccagcctc ggggctgccg cagggggacg gctgccttcg ggggggacgg ggcagggcgg
     2941 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     3001 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     3061 gcaaaggcta gtaatacgac tcactatagg gctagcgcca ccatggtgag caagggcgag
     3121 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     3181 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     3241 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     3301 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     3361 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     3421 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     3481 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     3541 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     3601 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     3661 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     3721 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     3781 gccgccggga tcactctcgg catggacgag ctgtacaagg gatccggcgc aacaaacttc
     3841 tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatgtt ggggtttgtg
     3901 ggtcgggtgg ccgctgctcc ggcctccggg gccttgcgga gactcacccc ttcagcgtcg
     3961 ctgcccccag ctcagctctt actgcgggcc gctccgacgg cggtccatcc tgtcagggac
     4021 tatgcggcgc aaacatctga gagcggcgga ggcgggagcc ccggcgcggc cgcggactac
     4081 aaggatgacg acgataaagg tagcgtggat ctacgcacgc tcggctacag ccagcagcaa
     4141 caggagaaga tcaaaccgaa ggttcgttcg acagtggcgc agcaccacga ggcactggtc
     4201 ggccatgggt ttacacacgc gcacatcgtt gcgctcagcc aacacccggc agcgttaggg
     4261 accgtcgctg tcaagtatca ggacatgatc gcagcgttgc cagaggcgac acacgaagcg
     4321 atcgttggcg tcggcaaaca gtggtccggc gcacgcgctc tggaggcctt gctcacggtg
     4381 gcgggagagt tgagaggtcc accgttacag ttggacacag gccaacttct caagattgca
     4441 aaacgtggcg gcgtgaccgc agtggaggca gtgcatgcat ggcgcaatgc actgacgggt
     4501 gcccccctga acctgacccc ggagcaggtg gtggccatcg ctagtaatat tggtggcaaa
     4561 caggctcttg agacggttca gcgcctcctt ccagttctct gtcaagccca cggactcacc
     4621 ccagatcaag ttgtagcgat tgctagtaat attggtggca aacaggctct tgagacggtt
     4681 cagcgcctcc ttccagttct ctgtcaagcc cacggactca ccccagatca ggttgtagcg
     4741 attgctagta acaatggtgg caaacaggct cttgaaaccg tacagcgcct actgccagtt
     4801 ctctgtcaag cccacggtct gactccggag caagttgtag cgattgctag taacaatggt
     4861 ggcaaacagg ctcttgaaac cgtacagcgc ctactgccag ttctctgtca agcccacggt
     4921 ctgactccgg agcaagtggt agcgattgct agtaacaatg gtggcaaaca ggctcttgaa
     4981 accgtacagc gcctactgcc agttctctgt caagcccacg gtctgactcc ggagcaagtt
     5041 gtagcgattg ctagtaacaa tggtggcaaa caggctcttg aaaccgtaca gcgcctactg
     5101 ccagttctct gtcaagccca cggtctgact ccggagcaag ttgtagcgat tgctagtaac
     5161 aatggtggca aacaggctct tgaaaccgta cagcgcctac tgccagttct ctgtcaagcc
     5221 cacggtctga ctccggagca agttgtagcg attgctagta atgggggtgg caaacaggct
     5281 cttgagactg ttcagcgcct tctaccagtt ctctgtcaag cccacggcct gacgcccgag
     5341 caagttgtag caattgctag taatgggggt ggcaaacagg ctcttgagac tgttcagcgc
     5401 cttctaccag ttctctgtca agcccacggc ctgacgcccg agcaagttgt agcgattgct
     5461 agtaatgggg gtggcaaaca ggctcttgag actgttcagc gccttctacc agttctctgt
     5521 caagcccacg gcctgacgcc cgagcaagtt gtagcgattg ctagtaatat tggtggcaaa
     5581 caggctcttg agacggttca gcgcctcctt ccagttctct gtcaagccca cggactcacc
     5641 ccagatcaag ttgtagcgat tgctagtaat attggtggca aacaggctct tgagacggtt
     5701 cagcgcctcc ttccagttct ctgtcaagcc cacggactca ccccagatca agttgtagcg
     5761 atagccagta acaatggtgg caaacaggct cttgaaaccg tacagcgcct actgccagtt
     5821 ctctgtcaag cccacggtct gactccggag caagttgtag cgattgctag tcatgacggt
     5881 ggcaaacagg ctcttgaaac cgtccaacgc cttctaccag ttctctgtca agcccacgga
     5941 ctaaccccag cgcaagttgt agcgattgca agtaatgggg gcggcaggcc ggcgctggag
     6001 agcattgttg cccagttatc tcgccctgat ccggcgttgg ccgcgttgac caacgaccac
     6061 ctcgtcgcct tggcctgcct cggcggacgt cctgcgctgg atgcagtgaa aaagggattg
     6121 ggaggctccg gctcctacgc cctgggccca tatcagatct ctgccccaca gctgccagca
     6181 tacaacggac agaccgtggg cacattctac tatgtgaatg acgccggcgg cctggagtct
     6241 aaggtgttta gctccggcgg cccaaccccc taccctaact atgccaatgc aggacacgtg
     6301 gagggacaga gcgccctgtt catgcgggat aacggcatct ccgagggcct ggtgttccac
     6361 aacaatcccg agggcacctg cggcttttgc gtgaacatga ccgagacact gctgcccgag
     6421 aatgccaaga tgacagtggt gccacctgag ggatctggag gcagcaccaa cctgtctgac
     6481 atcatcgaga aggagacagg caagcagctg gtcatccagg agagcatcct gatgctgccc
     6541 gaagaagtcg aagaagtgat cggaaacaag cctgagagcg atatcctggt ccataccgcc
     6601 tacgacgaga gtaccgacga aaatgtgatg ctgctgacat ccgacgcccc agagtataag
     6661 ccctgggctc tggtcatcca ggattccaac ggagagaaca aaatcaaaat gctgtaatct
     6721 agagggcccg tttaaacgtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     6781 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6841 tgtatcatgc gttaactaaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6901 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6961 aaactcatca atgtatctta tcatgtctgg aattgactca aatgatgtca attagtctat
     7021 cagaagctca tctggtctcc cttccggggg acaagacatc cctgtttaat atttaaacag
     7081 cagtgttccc aaactgggtt cttatatccc ttgctctggt caaccaggtt gcagggtttc
     7141 ctgtcctcac aggaacgaag tccctaaaga aacagtggca gccaggttta gccccggaat
     7201 tgactggatt ccttttttag ggcccattgg tatggctttt tccccgtatc cccccaggtg
     7261 tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc
     7321 ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg
     7381 gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc
     7441 ctgggggctt tggggggggg ctgtccctga tatctataac aagaaaatat atatataata
     7501 agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa
     7561 aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat
     7621 cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat
     7681 gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa
     7741 tgcatgcgtc aattttacgc agactatctt tctagggtta atctagctgc atcaggatca
     7801 tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg
     7861 ggaggaagaa gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag agtttgccga
     7921 ggatgactgc tgctgcattg acgttgagcg aaaacgcacg tttaccatga tgattcggga
     7981 aggtgtggcc atgcacgcct ttaacggtga actgttcgtt caggccacct gggataccag
     8041 ttcgtcgcgg cttttccgga cacagttccg gatggtcagc ccgaagcgca tcagcaaccc
     8101 gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg cagattaatg acagcggtgc
     8161 ggcgctggga tattacgtca gcgaggacgg gtatcctggc tggatgccgc agaaatggac
     8221 atggataccc cgtgagttac ccggcgggcg cgcttggcgt aatcatggtc atagctgttt
     8281 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8341 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8401 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8461 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8521 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8581 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8641 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8701 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8761 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8821 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8881 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8941 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9001 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9061 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     9121 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9181 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9241 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9301 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9361 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9421 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9481 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9541 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9601 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9661 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9721 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9781 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9841 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9901 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9961 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10021 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10081 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10141 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10201 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10261 agggcgacac ggaaatgttg aatactcat
//
