LOCUS       pAAV-PRSX8-jGCaM        5362 bp DNA     circular SYN 10-OCT-2023
DEFINITION  jGCaMP8m expression in noradrenergic neurons under control of the 
            PRSx8 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-PRSx8-jGCaMP8m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 10, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5362
                     /label=/dnas_title=pAAV-PRSX8-jGCaMP8m
                     /note="/dnas_title=pAAV-PRSX8-jGCaMP8m"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    165..186
                     /label=nonstandard type: PCR_primer
                     /note="PRSX8-1c"
     promoter        166..396
                     /label=PRSX8-Promoter
     misc_feature    180..202
                     /label=1st
                     /label=nonstandard type: loci
     misc_feature    203..225
                     /label=2nd
                     /label=nonstandard type: loci
     misc_feature    227..248
                     /label=3rd
                     /label=nonstandard type: loci
     misc_feature    249..270
                     /label=4th
                     /label=nonstandard type: loci
     misc_feature    295..317
                     /label=6th
                     /label=nonstandard type: loci
     misc_feature    318..340
                     /label=7th
                     /label=nonstandard type: loci
     misc_feature    341..362
                     /label=8th
                     /label=nonstandard type: loci
     CDS             427..1695
                     /label=jGCaMP8m
                     /note="jGCaMP8m"
     CDS             430..447
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     729..750
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(856..877)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1117..1136)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1708..2296
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(1761..1781)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     misc_feature    complement(1801..1822)
                     /direction=LEFT
                     /label=nonstandard type: PCR_primer
                     /note="WPRE-2nc"
                     /note="WPRE-2nc"
     CDS             complement(2179..2190)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2465..2546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2502..2521)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2556..2575
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   2625..2765
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      2840..3295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2927..2946)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3137..3158
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3312..3331)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     3431..3453
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3491..3509)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        3577..3681
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3682..4542
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3900..3919)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4713..5301
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5202..5221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaat taaaaacgcg tattagcttc
      181 cgctagacaa atgtgattac ccccgctaga caaatgtgat tacccgcgct agacaaatgt
      241 gattaccccg ctagacaaat gtgattaccc cccgctagac aaatgtgatt acccccgcta
      301 gacaaatgtg attacccgcg ctagacaaat gtgattaccc cgctagacaa atgtgattac
      361 ccccgaccag ggcataaatg gccaggtggg accagagata tcgctagcaa ggatccaccc
      421 gccaccatgc atcatcacca tcatcacacg cgtcgcaaga agaccttcaa ggaggtggcc
      481 accgccgtga agatcatcgg ccgcctgatg ggcctgaaga tcaacgtcta tatcaaggcc
      541 gacaagcaga agaacggcat caaggcgaac ttccacatcc gccacaacat cgaggacggc
      601 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      661 ctgcccgaca accactacct gagcgtggag tccaaacttt cgaaagaccc caacgagaag
      721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      781 gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc
      841 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      901 tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      961 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1021 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1081 gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1141 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1201 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacctgcc ggaccaactg
     1261 actgaagagc agatcgcaga atacaaagag gctttctccc tatttgacaa ggacggggat
     1321 gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca gaaccccaca
     1381 gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac
     1441 ttccctgagt tcctgacaat gatggcaaga aaaatgaaat acagggacac ggaagaagaa
     1501 attagagaag cgttcggtgt gtttgataag gatggcaatg gctacatcag tgcagcagag
     1561 cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt tgatgaaatg
     1621 atcagggaag cagacatcga tggggatggt caggtaaact acgaagagtt tgtacaaatg
     1681 atgacagcga agtaaaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     1741 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     1801 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     1861 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     1921 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     1981 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2041 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2101 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg
     2161 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2221 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     2281 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctgtgat agcggccatc
     2341 aagctggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     2401 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     2461 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2521 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2581 atcagcttat cgataccgca tgcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat
     2641 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     2701 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     2761 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     2821 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2881 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2941 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3001 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     3061 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3121 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3181 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3241 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     3301 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     3361 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3421 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3481 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     3541 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3601 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3661 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3721 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3781 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3841 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3901 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     3961 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4021 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4081 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4141 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4201 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4261 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4321 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4381 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4441 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4501 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4561 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4621 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4681 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4741 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4801 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     4861 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4921 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4981 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5041 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5101 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5161 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5221 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5281 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5341 tgctggcctt ttgctcacat gt
//
