LOCUS       Exported                4201 bp DNA     circular SYN 07-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 13, 2023 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     This file was created by DNADynamo
            www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     source          1..4201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /label=AmpR promoter
                     /note="AmpR promoter"
     CDS             126..986
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1157..1745
                     /label=ori
                     /note="ori"
     protein_bind    1910..1931
                     /label=CAP binding site
                     /note="CAP binding site"
     promoter        1946..1976
                     /label=lac promoter
                     /note="lac promoter"
     misc_feature    1958..1976
                     /label=PhD_seq_F
                     /note="PhD_seq_F"
     protein_bind    1984..2000
                     /label=lac operator
                     /note="lac operator"
     CDS             2025..2087
                     /codon_start=1
                     /label=Leader sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAM"
     CDS             2089..2262
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(2109..2125)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2277..2321
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             2325..2342
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2343..2348
                     /codon_start=1
                     /label=BAP-His
                     /translation="*"
     CDS             2381..2402
                     /codon_start=1
                     /label=Myc
                     /note="Myc"
                     /translation="THLRRGS"
     misc_feature    complement(2461..2480)
                     /label=PhD_seq_R
                     /note="PhD_seq_R"
     rep_origin      complement(3767..4195)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc gcttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1861 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     1921 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     1981 gaattgtgag cggataacaa tttgaattca aggagacagt cataatgaaa tacctattgc
     2041 ctacggcggc cgctggattg ttattactcg cggcccagcc ggccatggat gaccatgatt
     2101 acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     2161 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     2221 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcca tcctcaggct
     2281 tgaatgatat ttttgaggca caaaagattg aatggcatga aaaacaccat caccatcacc
     2341 attaataacc accatcatgg cgcagaacaa accagccggc actcatctca gaagaggatc
     2401 tgtcttaggc cgaaactgtt gaaagttgtt tagcaaaacc tcatacagaa aattcattta
     2461 ctaacgtctg gaaagacgac aaaactttag atcgttacgc taactatgag ggctgtctgt
     2521 ggaatgctac aggcgttgtg gtttgtactg gtgacgaaac tcagtgttac ggtacatggg
     2581 ttcctattgg gcttgctatc cctgaaaatg agggtggtgg ctctgagggt ggcggttctg
     2641 agggtggcgg ttctgagggt ggcggtacta aacctcctga gtacggtgat acacctattc
     2701 cgggctatac ttatatcaac cctctcgacg gcacttatcc gcctggtact gagcaaaacc
     2761 ccgctaatcc taatccttct cttgaggagt ctcagcctct taatactttc atgtttcaga
     2821 ataataggtt ccgaaatagg cagggtgcat taactgttta tacgggcact gttactcaag
     2881 gcactgaccc cgttaaaact tattaccagt acactcctgt atcatcaaaa gccatgtatg
     2941 acgcttactg gaacggtaaa ttcagagact gcgctttcca ttctggcttt aatgaggatc
     3001 cattcgtttg tgaatatcaa ggccaatcgt ctgacctgcc tcaacctcct gtcaatgctg
     3061 gcggcggctc tggtggtggt tctggtggcg gctctgaggg tggcggctct gagggtggcg
     3121 gttctgaggg tggcggctct gagggtggcg gttccggtgg cggctccggt tccggtgatt
     3181 ttgattatga aaaaatggca aacgctaata agggggctat gaccgaaaat gccgatgaaa
     3241 acgcgctaca gtctgacgct aaaggcaaac ttgattctgt cgctactgat tacggtgctg
     3301 ctatcgatgg tttcattggt gacgtttccg gccttgctaa tggtaatggt gctactggtg
     3361 attttgctgg ctctaattcc caaatggctc aagtcggtga cggtgataat tcacctttaa
     3421 tgaataattt ccgtcaatat ttaccttctt tgcctcagtc ggttgaatgt cgcccttatg
     3481 tctttggcgc tggtaaacca tatgaatttt ctattgattg tgacaaaata aacttattcc
     3541 gtggtgtctt tgcgtttctt ttatatgttg ccacctttat gtatgtattt tcgacgtttg
     3601 ctaacatact gcgtaataag gagtcttaag ctagctaaca gtcctatgaa tcaactactt
     3661 agatggtatt agtgacctgt aacagagcat tagcgcaagg tgatttttgt cttcttgcgc
     3721 taattttttg tcatcaaacc tgtcgcactc cttaatattt tgttaaaatt cgcgttaaat
     3781 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     3841 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     3901 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     3961 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     4021 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     4081 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     4141 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     4201 g
//
