LOCUS       Exported               14423 bp ds-DNA     circular SYN 11-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 
            addgene-plasmid-24129-sequence-11312.ape 3rd generation Lentiviral 
            vector for bi-cistronic expression of EGFP and the gene of interest.
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="note: truncated 5' long terminal repeat (LTR) from 
                     HIV-1"
     misc_feature    463..588
                     /label=HIV-1 Psi
                     /note="note: packaging signal of human immunodeficiency 
                     virus type 1"
     misc_feature    1081..1314
                     /label=RRE
                     /note="note: The Rev response element (RRE) of HIV-1 allows
                     for Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1499..1543
                     /label=gp41 peptide
                     /note="product: antigenic peptide corresponding to amino 
                     acids 655 to 669 of the HIV envelope protein gp41 (Lutje 
                     Hulsik et al., 2013) note: recognized by the 2H10 
                     single-chain llama nanobody translation: KNEQELLELDKWASL"
     CDS             1499..1543
     misc_feature    1841..1958
                     /label=cPPT/CTS
                     /note="note: central polypurine tract and central 
                     termination sequence of HIV-1"
     promoter        2023..3234
                     /label=UbC promoter note: human ubiquitin C promoter
                     /label=UbC promoter
                     /note="note: human ubiquitin C promoter"
     primer_bind     3071..3090
                     /label=hUBCpro-F
                     /note="note: Human Ubiquitin C (UbC) promoter, forward 
                     primer"
     misc_feature    3267..3276
                     /label=vertebrate consensus sequence for strong initia
                     /label=vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)
                     /note="regulatory_class: other"
     CDS             3273..3989
                     /codon_start=1
                     /label=EGFP
                     /note="product: the original enhanced GFP (Yang et al., 
                     1996) note: mammalian codon-optimized translation: 
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWP
                     TLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD
                     TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSV
                     QLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDE
                     LYK"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3999..4055
                     /label=P2A
                     /note="product: 2A peptide from porcine teschovirus-1 
                     polyprotein note: Eukaryotic ribosomes fail to insert a 
                     peptide bond between the Gly and Pro residues, yielding 
                     separate polypeptides. translation: ATNFSLLKQAGDVEENPGP"
     CDS             4056..6836
                     /codon_start=1
                     /label=BCR
                     /label=BCR Portion of BCRABL
                     /translation="MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQELERCKASIRRL
                     EQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQAPDGASEPRASASRPQPAPADG
                     ADPPPAEEPEARPDGEGSPGKARPGTARRPGAAASGERDDRGPPASVAALRSNFERIRK
                     GHGQPGADAEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQHGAGSS
                     VGDASRPPYRGRSSESSCGVDGDYEDAELNPRFLKDNLIDANGGSRPPWPPLEYQPYQS
                     IYVGGMMEGEGKGPLLRSQSTSEQEKRLTWPRRSYSPRSFEDCGGGYTPDCSSNENLTS
                     SEEDFSSGQSSRVSPSPTTYRMFRDKSRSPSQNSQQSFDSSSPPTPQCHKRHRHCPVVV
                     SEATIVGVRKTGQIWPNDGEGAFHGDADGSFGTPPGYGCAADRAEEQRRHQDGLPYIDD
                     SPSSSPHLSSKGRGSRDALVSGALESTKASELDLEKGLEMRKWVLSGILASEETYLSHL
                     EALLLPMKPLKAAATTSQPVLTSQQIETIFFKVPELYEIHKEFYDGLFPRVQQWSHQQR
                     VGDLFQKLASQLGVYRAFVDNYGVAMEMAEKCCQANAQFAEISENLRARSNKDAKDPTT
                     KNSLETLLYKPVDRVTRSTLVLHDLLKHTPASHPDHPLLQDALRISQNFLSSINEEITP
                     RRQSMTVKKGEHRQLLKDSFMVELVEGARKLRHVFLFTDLLLCTKLKKQSGGKTQQYDC
                     KWYIPLTDLSFQMVDELEAVPNIPLVPDEELDALKIKISQIKNDIQREKRANKGSKATE
                     RLKKKLSEQESLLLLMSPSMAFRVHSRNGKSYTFLISSDYERAEWRENIREQQKKCFRS
                     FSLTSVELQMLTNSCVKLQTVHSIPLTINKEDDESPGLYGFLNVIVHSATGFKQSS"
     CDS             6837..10151
                     /codon_start=1
                     /label=ABL1
                     /label=ABL portion of BCR-ABL
                     /translation="KALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYD
                     FVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHG
                     PVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSE
                     SRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKL
                     GGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTR
                     EPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAA
                     RNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAF
                     GVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRP
                     SFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRD
                     TTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSA
                     LIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPK
                     PSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSAS
                     CVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGT
                     VTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGK
                     GSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKP
                     APPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLK
                     KPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKT
                     RQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSI
                     QQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR"
     misc_feature    6948..7118
                     /label=SH3 Domain (Smart)
     misc_feature    7131..7382
                     /label=SH2 domain (SMART)
     misc_feature    7482..8240
                     /label=Kinase domain
     misc_feature    10270..10858
                     /label=WPRE
                     /note="note: woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10741..10752)
                     /label=Factor Xa site
                     /note="product: Factor Xa recognition and cleavage site 
                     translation: IEGR"
     LTR             10949..11182
                     /label=3' LTR (Delta-U3)
                     /note="note: self-inactivating 3' long terminal repeat 
                     (LTR) fromHIV-1"
     polyA_signal    11254..11375
                     /label=SV40 poly(A) signal
                     /note="note: SV40 polyadenylation signal"
     rep_origin      11415..11550
                     /label=SV40 ori
                     /note="note: SV40 origin of replication"
     promoter        complement(11571..11589)
                     /label=T7 promoter note: promoter for bacteriophage T7
                     /label=T7 promoter
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     rep_origin      11757..12212
                     /label=f1 ori
                     /note="direction: RIGHT note: f1 bacteriophage origin of 
                     replication; arrow indicates direction of (+) strand 
                     synthesis"
     promoter        12238..12342
                     /label=AmpR promoter gene: bla
                     /label=AmpR promoter
                     /note="gene: bla"
     CDS             12343..13203
                     /label=AmpR
                     /note="gene: bla product: beta-lactamase note: confers 
                     resistance to ampicillin, carbenicillin, andrelated 
                     antibiotics translation: 
                     MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFR
                     PEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVR
                     ELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERD
                     TTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAG
                     ERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     rep_origin      13374..13962
                     /label=ori
                     /note="direction: RIGHT note: high-copy-number 
                     ColE1/pMB1/pBR322/pUC origin of replication"
     protein_bind    14250..14271
                     /label=CAP binding site
                     /note="bound_moiety: E. coli catabolite activator protein 
                     note: CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        14286..14316
                     /label=lac promoter note: promoter for the E. coli lac
                     /label=lac promoter
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    14324..14340
                     /label=lac operator
                     /note="bound_moiety: lac repressor encoded by lacI note: 
                     The lac repressor binds to the lac operator to inhibit 
                     transcription in E. coli. This inhibition can be relieved 
                     by adding lactose or isopropyl-beta-D-thiogalactopyranoside
                     (IPTG)."
     promoter        14385..14403
                     /label=T3 promoter note: promoter for bacteriophage T3
                     /label=T3 promoter
                     /note="note: promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     1801 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1861 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1921 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1981 gagatccagt ttggttaatt aacccgtgtc ggctccagat ctggcctccg cgccgggttt
     2041 tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
     2101 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
     2161 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
     2221 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
     2281 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
     2341 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
     2401 atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc
     2461 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
     2521 tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt
     2581 tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg
     2641 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     2701 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     2761 gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg
     2821 cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg
     2881 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     2941 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     3001 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     3061 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     3121 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     3181 tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgaagct
     3241 tgggctgcag gtccgatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     3301 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3361 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3421 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     3481 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3541 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3601 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3661 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3721 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     3781 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3841 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     3901 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3961 tcactctcgg catggacgag ctgtacaagg gatctggagc aacaaacttc tcactactca
     4021 aacaagcagg tgacgtggag gagaatcccg ggcctatggt ggacccggtg ggcttcgcgg
     4081 aggcgtggaa ggcgcagttc ccggactcag agcccccgcg catggagctg cgctcagtgg
     4141 gcgacatcga gcaggagctg gagcgctgca aggcctccat tcggcgcctg gagcaggagg
     4201 tgaaccagga gcgcttccgc atgatctacc tgcagacgtt gctggccaag gaaaagaaga
     4261 gctatgaccg gcagcgatgg ggcttccggc gcgcggcgca ggcccccgac ggcgcctccg
     4321 agccccgagc gtccgcgtcg cgcccgcagc cagcgcccgc cgacggagcc gacccgccgc
     4381 ccgccgagga gcccgaggcc cggcccgacg gcgagggttc tccgggtaag gccaggcccg
     4441 ggaccgcccg caggcccggg gcagccgcgt cgggggaacg ggacgaccgg ggaccccccg
     4501 ccagcgtggc ggcgctcagg tccaacttcg agcggatccg caagggccat ggccagcccg
     4561 gggcggacgc cgagaagccc ttctacgtga acgtcgagtt tcaccacgag cgcggcctgg
     4621 tgaaggtcaa cgacaaagag gtgtcggacc gcatcagctc cctgggcagc caggccatgc
     4681 agatggagcg caaaaagtcc cagcacggcg cgggctcgag cgtgggggat gcatccaggc
     4741 ccccttaccg gggacgctcc tcggagagca gctgcggcgt cgacggcgac tacgaggacg
     4801 ccgagttgaa cccccgcttc ctgaaggaca acctgatcga cgccaatggc ggtagcaggc
     4861 ccccttggcc gcccctggag taccagccct accagagcat ctacgtcggg ggcatgatgg
     4921 aaggggaggg caagggcccg ctcctgcgca gccagagcac ctctgagcag gagaagcgcc
     4981 ttacctggcc ccgcaggtcc tactcccccc ggagttttga ggattgcgga ggcggctata
     5041 ccccggactg cagctccaat gagaacctca cctccagcga ggaggacttc tcctctggcc
     5101 agtccagccg cgtgtcccca agccccacca cctaccgcat gttccgggac aaaagccgct
     5161 ctccctcgca gaactcgcaa cagtccttcg acagcagcag tccccccacg ccgcagtgcc
     5221 ataagcggca ccggcactgc ccggttgtcg tgtccgaggc caccatcgtg ggcgtccgca
     5281 agaccgggca gatctggccc aacgatggcg agggcgcctt ccatggagac gcagatggct
     5341 cgttcggaac accacctgga tacggctgcg ctgcagaccg ggcagaggag cagcgccggc
     5401 accaagatgg gctgccctac attgatgact cgccctcctc atcgccccac ctcagcagca
     5461 agggcagggg cagccgggat gcgctggtct cgggagccct ggagtccact aaagcgagtg
     5521 agctggactt ggaaaagggc ttggagatga gaaaatgggt cctgtcggga atcctggcta
     5581 gcgaggagac ttacctgagc cacctggagg cactgctgct gcccatgaag cctttgaaag
     5641 ccgctgccac cacctctcag ccggtgctga cgagtcagca gatcgagacc atcttcttca
     5701 aagtgcctga gctctacgag atccacaagg agttctatga tgggctcttc ccccgcgtgc
     5761 agcagtggag ccaccagcag cgggtgggcg acctcttcca gaagctggcc agccagctgg
     5821 gtgtgtaccg ggccttcgtg gacaactacg gagttgccat ggaaatggct gagaagtgct
     5881 gtcaggccaa tgctcagttt gcagaaatct ccgagaacct gagagccaga agcaacaaag
     5941 atgccaagga tccaacgacc aagaactctc tggaaactct gctctacaag cctgtggacc
     6001 gtgtgacgag gagcacgctg gtcctccatg acttgctgaa gcacactcct gccagccacc
     6061 ctgaccaccc cttgctgcag gacgccctcc gcatctcaca gaacttcctg tccagcatca
     6121 atgaggagat cacaccccga cggcagtcca tgacggtgaa gaagggagag caccggcagc
     6181 tgctgaagga cagcttcatg gtggagctgg tggagggggc ccgcaagctg cgccacgtct
     6241 tcctgttcac cgacctgctt ctctgcacca agctcaagaa gcagagcgga ggcaaaacgc
     6301 agcagtatga ctgcaaatgg tacattccgc tcacggatct cagcttccag atggtggatg
     6361 aactggaggc agtgcccaac atccccctgg tgcccgatga ggagctggac gctttgaaga
     6421 tcaagatctc ccagatcaag aatgacatcc agagagagaa gagggcgaac aagggcagca
     6481 aggctacgga gaggctgaag aagaagctgt cggagcagga gtcactgctg ctgcttatgt
     6541 ctcccagcat ggccttcagg gtgcacagcc gcaacggcaa gagttacacg ttcctgatct
     6601 cctctgacta tgagcgtgca gagtggaggg agaacatccg ggagcagcag aagaagtgtt
     6661 tcagaagctt ctccctgaca tccgtggagc tgcagatgct gaccaactcg tgtgtgaaac
     6721 tccagactgt ccacagcatt ccgctgacca tcaataagga agatgatgag tctccggggc
     6781 tctatgggtt tctgaatgtc atcgtccact cagccactgg atttaagcag agttcaaaag
     6841 cccttcagcg gccagtagca tctgactttg agcctcaggg tctgagtgaa gccgctcgtt
     6901 ggaactccaa ggaaaacctt ctcgctggac ccagtgaaaa tgaccccaac cttttcgttg
     6961 cactgtatga ttttgtggcc agtggagata acactctaag cataactaaa ggtgaaaagc
     7021 tccgggtctt aggctataat cacaatgggg aatggtgtga agcccaaacc aaaaatggcc
     7081 aaggctgggt cccaagcaac tacatcacgc cagtcaacag tctggagaaa cactcctggt
     7141 accatgggcc tgtgtcccgc aatgccgctg agtatctgct gagcagcggg atcaatggca
     7201 gcttcttggt gcgtgagagt gagagcagtc ctggccagag gtccatctcg ctgagatacg
     7261 aagggagggt gtaccattac aggatcaaca ctgcttctga tggcaagctc tacgtctcct
     7321 ccgagagccg cttcaacacc ctggccgagt tggttcatca tcattcaacg gtggccgacg
     7381 ggctcatcac cacgctccat tatccagccc caaagcgcaa caagcccact gtctatggtg
     7441 tgtcccccaa ctacgacaag tgggagatgg aacgcacgga catcaccatg aagcacaagc
     7501 tgggcggggg ccagtacggg gaggtgtacg agggcgtgtg gaagaaatac agcctgacgg
     7561 tggccgtgaa gaccttgaag gaggacacca tggaggtgga agagttcttg aaagaagctg
     7621 cagtcatgaa agagatcaaa caccctaacc tggtgcagct ccttggggtc tgcacccggg
     7681 agcccccgtt ctatatcatc actgagttca tgacctacgg gaacctcctg gactacctga
     7741 gggagtgcaa ccggcaggag gtgaacgccg tggtgctgct gtacatggcc actcagatct
     7801 cgtcagccat ggagtacctg gagaagaaaa acttcatcca cagagatctt gctgcccgaa
     7861 actgcctggt aggggagaac cacttggtga aggtagctga ttttggcctg agcaggttga
     7921 tgacagggga cacctacaca gcccatgctg gagccaagtt ccccatcaaa tggactgcac
     7981 ccgagagcct ggcctacaac aagttctcca tcaagtccga cgtctgggca tttggagtat
     8041 tgctttggga aattgctacc tatggcatgt ccccttaccc gggaattgac ctgtcccagg
     8101 tgtatgagct gctagagaag gactaccgca tggagcgccc agaaggctgc ccagagaagg
     8161 tctatgaact catgcgagca tgttggcagt ggaatccctc tgaccggccc tcctttgctg
     8221 aaatccacca agcctttgaa acaatgttcc aggaatccag tatctcagac gaagtggaaa
     8281 aggagctggg gaaacaaggc gtccgtgggg ctgtgagtac cttgctgcag gccccagagc
     8341 tgcccaccaa gacgaggacc tccaggagag ctgcagagca cagagacacc actgacgtgc
     8401 ctgagatgcc tcactccaag ggccagggag agagcgatcc tctggaccat gagcctgccg
     8461 tgtctccatt gctccctcga aaagagcgag gtcccccgga gggcggcctg aatgaagatg
     8521 agcgccttct ccccaaagac aaaaagacca acttgttcag cgccttgatc aagaagaaga
     8581 agaagacagc cccaacccct cccaaacgca gcagctcctt ccgggagatg gacggccagc
     8641 cggagcgcag aggggccggc gaggaagagg gccgagacat cagcaacggg gcactggctt
     8701 tcaccccctt ggacacagct gacccagcca agtccccaaa gcccagcaat ggggctgggg
     8761 tccccaatgg agccctccgg gagtccgggg gctcaggctt ccggtctccc cacctgtgga
     8821 agaagtccag cacgctgacc agcagccgcc tagccaccgg cgaggaggag ggcggtggca
     8881 gctccagcaa gcgcttcctg cgctcttgct ccgcctcctg cgttccccat ggggccaagg
     8941 acacggagtg gaggtcagtc acgctgcctc gggacttgca gtccacggga agacagtttg
     9001 actcgtccac atttggaggg cacaaaagtg agaagccggc tctgcctcgg aagagggcag
     9061 gggagaacag gtctgaccag gtgacccgag gcacagtaac gcctcccccc aggctggtga
     9121 aaaagaatga ggaagctgct gatgaggtct tcaaagacat catggagtcc agcccgggct
     9181 ccagcccgcc caacctgact ccaaaacccc tccggcggca ggtcaccgtg gcccctgcct
     9241 cgggcctccc ccacaaggaa gaagctggaa agggcagtgc cttagggacc cctgctgcag
     9301 ctgagccagt gacccccacc agcaaagcag gctcaggtgc accagggggc accagcaagg
     9361 gccccgccga ggagtccaga gtgaggaggc acaagcactc ctctgagtcg ccagggaggg
     9421 acaaggggaa attgtccagg ctcaaacctg ccccgccgcc cccaccagca gcctctgcag
     9481 ggaaggctgg aggaaagccc tcgcagagcc cgagccagga ggcggccggg gaggcagtcc
     9541 tgggcgcaaa gacaaaagcc acgagtctgg ttgatgctgt gaacagtgac gctgccaagc
     9601 ccagccagcc gggagagggc ctcaaaaagc ccgtgctccc ggccactcca aagccacagt
     9661 ccgccaagcc gtcggggacc cccatcagcc cagcccccgt tccctccacg ttgccatcag
     9721 catcctcggc cctggcaggg gaccagccgt cttccaccgc cttcatccct ctcatatcaa
     9781 cccgagtgtc tcttcggaaa acccgccagc ctccagagcg gatcgccagc ggcgccatca
     9841 ccaagggcgt ggtcctggac agcaccgagg cgctgtgcct cgccatctct aggaactccg
     9901 agcagatggc cagccacagc gcagtgctgg aggccggcaa aaacctctac acgttctgcg
     9961 tgagctatgt ggattccatc cagcaaatga ggaacaagtt tgccttccga gaggccatca
    10021 acaaactgga gaataatctc cgggagcttc agatctgccc ggcgacagca ggcagtggtc
    10081 cagcggccac tcaggacttc agcaagctcc tcagttcggt gaaggaaatc agtgacatag
    10141 tgcagaggta gtctagagga tccggctccg gcgagggcag gggaagtcta ctaacatgcg
    10201 gggacgtgga ggaaaatccc ggcccagcta gcgtgtgatc agaattcgtt ccggagtcgt
    10261 cgactcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    10321 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    10381 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    10441 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    10501 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    10561 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    10621 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
    10681 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    10741 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    10801 ccgcatcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
    10861 ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
    10921 agccactttt taaaagaaaa ggggggactg gaagggctac gtaactccca acgaagacaa
    10981 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    11041 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    11101 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    11161 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
    11221 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
    11281 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    11341 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
    11401 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    11461 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    11521 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
    11581 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
    11641 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    11701 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
    11761 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    11821 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    11881 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    11941 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    12001 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    12061 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    12121 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    12181 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
    12241 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    12301 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
    12361 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
    12421 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
    12481 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
    12541 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
    12601 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
    12661 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
    12721 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
    12781 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
    12841 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
    12901 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    12961 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    13021 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    13081 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    13141 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    13201 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    13261 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    13321 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    13381 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    13441 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    13501 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    13561 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    13621 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    13681 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    13741 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    13801 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    13861 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctc acttgagcgt
    13921 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    13981 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    14041 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    14101 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    14161 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
    14221 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    14281 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    14341 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
    14401 agggaacaaa agctggagct gca
//
