LOCUS       Exported                6973 bp ds-DNA     circular SYN 07-JUL-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 11, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4877..5872
                     /organism="Escherichia coli"
                     /strain="F2513"
                     /mol_type="genomic DNA"
                     /label=derived from LPS core biosynthesis region;
                     R4 c...
                     /label=derived from LPS core biosynthesis region; R4 core 
                     type(1)
                     /note="derived from LPS core biosynthesis region; R4 core
                     type"
                     /note="/locus_tag=derived from LPS core biosynthesis 
                     region; R4
                     core type(1)"
                     /db_xref="taxon:562"
     source          5873..6951
                     /organism="Escherichia coli"
                     /strain="F2513"
                     /mol_type="genomic DNA"
                     /label=derived from LPS core biosynthesis region;
                     R4 c...
                     /label=derived from LPS core biosynthesis region; R4 core
                     type
                     /note="derived from LPS core biosynthesis region; R4 core
                     type"
                     /note="/locus_tag=derived from LPS core biosynthesis 
                     region; R4
                     core type"
                     /db_xref="taxon:562"
     misc_feature    complement(23..40)
                     /locus_tag="pTrcHis_rev_primer"
                     /label=pTrcHis_rev_primer
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    complement(23..40)
                     /locus_tag="pBAD_rev_primer"
                     /label=pBAD_rev_primer
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    complement(63..82)
                     /locus_tag="Primer pBAD verif R (NAT)"
                     /label=Primer pBAD verif R (NAT)
     terminator      73..230
                     /locus_tag="rrnB_terminator"
                     /label=rrnB_terminator
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     terminator      196..239
                     /locus_tag="rrnB_T1_terminator"
                     /label=rrnB_T1_terminator
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     terminator      371..398
                     /locus_tag="rrnB_T2_terminator"
                     /label=rrnB_T2_terminator
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     promoter        439..467
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     gene            509..1369
                     /gene="Ampicillin"
                     /locus_tag="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     CDS             509..1369
                     /codon_start=1
                     /locus_tag="ORF frame 3"
                     /label=ORF frame 3
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1370..1389
                     /locus_tag="Primer oKN576"
                     /label=Primer oKN576
     rep_origin      1428..1727
                     /locus_tag="f1_origin"
                     /label=f1_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     rep_origin      2572..3399
                     /locus_tag="p15A_origin"
                     /label=p15A_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    3444..3463
                     /locus_tag="pBRrevBam_primer"
                     /label=pBRrevBam_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     CDS             complement(3643..4572)
                     /codon_start=1
                     /locus_tag="ORF frame 1"
                     /label=ORF frame 1
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIEA
                     NGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPE
                     AREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGR
                     YSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVC
                     LSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRV
                     FKKCTGASPSEFRAGCEEKVNDVAVKLS"
     misc_feature    complement(3643..4521)
                     /locus_tag="AraC"
                     /label=AraC
     misc_feature    4551..4566
                     /locus_tag="araO2"
                     /label=araO2
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    4708..4719
                     /locus_tag="araO1"
                     /label=araO1
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    4750..4763
                     /locus_tag="CAP_BS"
                     /label=CAP_BS
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    4755..4774
                     /locus_tag="pBAD_fwd_primer"
                     /label=pBAD_fwd_primer
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    4760..4798
                     /locus_tag="AraI1I2"
                     /label=AraI1I2
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     promoter        4795..4823
                     /locus_tag="ARA_promoter"
                     /label=ARA_promoter
                     /note="/translation=MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAG
                     LTPIE 
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH 
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG 
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA 
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL 
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
     misc_feature    complement(4820..4841)
                     /locus_tag="Primer oKN577"
                     /label=Primer oKN577
     misc_feature    complement(4834..4853)
                     /locus_tag="Primer oKN602 binding site"
                     /label=Primer oKN602 binding site
     misc_feature    complement(4845..4876)
                     /locus_tag="Primer pBAD30_R_bb"
                     /label=Primer pBAD30_R_bb
     misc_feature    4857..4906
                     /locus_tag="oNA412"
                     /label=oNA412
     gene            4877..5872
                     /gene="waaT"
                     /locus_tag="waaT"
                     /label=waaT
     CDS             4877..5872
                     /codon_start=1
                     /transl_table=11
                     /gene="waaT"
                     /locus_tag="UDP-galactose:(glucosyl) LPS 
                     alpha1,2-galactosyltransferase WaaT"
                     /product="UDP-galactose:(glucosyl) LPS 
                     alpha1,2-galactosyltransferase WaaT"
                     /label=UDP-galactose:(glucosyl) LPS alpha1,2-galactosy...
                     /label=UDP-galactose:(glucosyl) LPS 
                     alpha1,2-galactosyltransferase WaaT
                     /note="similar to former RfaJ"
                     /protein_id="AAC69686.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSVL
                     INNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSYAT
                     YFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVVPDNDSTQAACAKR
                     LNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNM
                     NNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYF
                     NIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     misc_feature    complement(5848..5892)
                     /locus_tag="oNA534"
                     /label=oNA534
     misc_feature    5853..5892
                     /locus_tag="oNA535"
                     /label=oNA535
     CDS             5898..6926
                     /codon_start=1
                     /transl_table=11
                     /gene="waaW"
                     /locus_tag="UDP-galactose:(galactosyl) LPS 
                     alpha1,2-galactosyltransferase WaaW"
                     /product="UDP-galactose:(galactosyl) LPS 
                     alpha1,2-galactosyltransferase WaaW"
                     /label=UDP-galactose:(galactosyl) LPS alpha1,2-galacto...
                     /label=UDP-galactose:(galactosyl) LPS 
                     alpha1,2-galactosyltransferase WaaW
                     /protein_id="AAC69684.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQSV
                     VMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQWSY
                     ATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQEKP
                     AKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDILNIL
                     FFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWTNYPCQ
                     KYFNEAYQASCWNDVAFIPATNEKQYQVKSRHLKRNGNIASSFYYFMLYYSKKIARKHF
                     SK"
     gene            5898..6926
                     /gene="waaW"
                     /locus_tag="waaW"
                     /label=waaW
     misc_feature    complement(6924..6971)
                     /locus_tag="oNA536"
                     /label=oNA536
     misc_feature    6972..6973
                     /locus_tag="Primer pBAD30_F_bb"
                     /label=Primer pBAD30_F_bb
ORIGIN
        1 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
       61 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
      121 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
      181 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      241 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
      301 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
      361 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
      421 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      481 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      541 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      601 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      661 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      721 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      781 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      841 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      901 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      961 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1021 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     1081 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1141 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1201 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1261 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1321 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1381 agtttactca tatatacttt agattgattt acgcgccctg tagcggcgca ttaagcgcgg
     1441 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     1501 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     1561 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     1621 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     1681 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaacttga acaacactca
     1741 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     1801 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     1861 caatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2101 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     2161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt caggcatttg
     2221 agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat
     2281 aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt
     2341 tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac
     2401 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     2461 tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc
     2521 agcggcatca gcaccttgtc gccttgcgta taatatttgc cgctagcgga gtgtatactg
     2581 gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa
     2641 aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca
     2701 ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg
     2761 gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa
     2821 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
     2881 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct
     2941 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     3001 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     3061 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
     3121 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
     3181 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
     3241 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
     3301 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
     3361 aaacgatctc aagaagatca tcttattaat cagataaaat atttgctcat gagcccgaag
     3421 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     3481 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatctgctc atgtttgaca
     3541 gcttatcatc gatgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
     3601 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc
     3661 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca
     3721 ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt
     3781 ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc
     3841 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg
     3901 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc
     3961 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt
     4021 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga
     4081 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg
     4141 ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag
     4201 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc
     4261 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg
     4321 tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt
     4381 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc
     4441 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg
     4501 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg
     4561 tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa
     4621 accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa
     4681 aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg
     4741 cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg
     4801 ctttttatcg caactctcta ctgtttctcc atacccgttt ttttgggcta gcgaattcga
     4861 gctctaggag gcaaaaatga atgaatttat aaaagaacgg ttttcgtatt tagccgataa
     4921 taaaaaagaa aacgccccag agctaaatgt ttcctacggt atcgataaga attttttgta
     4981 tggtgctggc gtttcaattt cttccgtttt gattaataat tcagatatta attttgtctt
     5041 tcatgttttc actgattatg tggatgatga ttatttaaag tcatttaatg aaacagcaaa
     5101 acaatttaat acctcaatta ttgtatattt aattgacccc aaatactttg ctgatctgcc
     5161 gacgtcacag ttttggtcgt acgcgacata cttcagggta ttgtcttttg aatatctgag
     5221 tgaaagtatt tccacactgc tgtatctgga tgccgatgtt gtttgtaaag gaagcctgaa
     5281 acctctcaca gaaattatat ttaaagatga gtttgctgcg gtcgttcctg acaatgatag
     5341 tactcaggcg gcatgtgcaa aacgtcttaa cattcccgaa atgaatggac gttatttcaa
     5401 tgcaggcgtt atctatgtca atcttaaaaa atggcatgaa gcaaatttga caccgtattt
     5461 actcacgctt ttacgaggtg aaactaaata tggctctctt aaatatttag atcaggatgc
     5521 gttgaatatc gcatttaata tgaataatat ctaccttgcg aaggattttg atactattta
     5581 taccctgaaa aacgaacttt atgatcgtag tcatcgaaag tatcagcaaa ccattaccga
     5641 taaaacagtg ttgattcact atacagggat aactaaacca tggcatagct gggctggata
     5701 tccgtctgca tcatacttta atatcgcgcg tgaacaatct ccctggaaga aatatcctct
     5761 taaagaggcg cggactgttg cagaaatgca gaaacaatat aagcatctgt ttgcccatgg
     5821 tgagtatata aaaggcataa cttcattaat taagtacaag cttaagaaat aaaccacaca
     5881 gtcaaaacgg aaccaacatg gatttattag ctgagagtat tactgaagtc gctgtctctg
     5941 gggaaattgc taacaccgat cgtgtgttaa atatcgctta cggtattgac cgtaattttt
     6001 tatttggtgc ggccgtatct atgcaatcag ttgttatgca taacccggac cttgcggtta
     6061 agtttcatct ctttaccgac tacattgatg aagattatct acaacgtgtt aacgctttta
     6121 ccagcaaaaa tgctaacgtt gaagtaagaa tttataaagt ctccagtgcc tttattgata
     6181 ttttccccag cctgaaacag tggtcttatg caacattctt ccgtttagtt gcgttccagt
     6241 atctgagtga aactattgaa aatctgttat atatcgatgc tgatgtcatc tgtaaaggct
     6301 cattagctgg attgcttgat atcaattttg atggcgataa gttcgcagct gttattaaag
     6361 atgtgccttt tatgcaggaa aaaccagcga agcgtctggc tatagaggga cttccaggta
     6421 attatttcaa cgccggtgta gtatatctgc agcttgaagc atgggcgaaa aatgatttta
     6481 tgaataaagc cattgctatg ctggcaagtg acccgcagca tacgaagtat aaatgccttg
     6541 atcaggatat tttaaatatt ctgttctttg gtcattgtat ttttattagc ggcgattatg
     6601 attgctttta tggcattgac tatgagttaa aaaataaaag cgatgaagat tataaaaaga
     6661 ccattaccga tgatactaag ctgattcatt atgttggcgt aacgaagccc tggaacgact
     6721 ggacgaatta tccctgccag aagtatttta atgaggctta tcaggcttct tgctggaatg
     6781 atgtggcgtt tattccagcc acgaatgaaa agcagtatca agtgaaatcc agacatttga
     6841 aaagaaatgg taatattgca tcttcatttt actatttcat gctctattat agtaagaaaa
     6901 tagcgcgtaa gcatttttcc aaataattat taagattaat cacccagaga tgcatgcaag
     6961 cttggctgtt ttg
//
