LOCUS       Exported                8412 bp ds-DNA     circular SYN 16-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 16, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        407..610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2405..2675
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2707..2745
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2839..2874
                     /label=MCS
     misc_feature    2893..3481
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3364..3375)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3484..3500)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             4006..4186
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4218..4442
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4488..4916
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4930..5259
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5110..5245
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5307..5354
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5373..5747
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5877..5998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6047..6063)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6071..6087
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6095..6125)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6140..6161
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6449..7037)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7208..8068)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8069..8173)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttaatgat ctcgagttta
     2401 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     2461 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2521 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2581 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     2641 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc
     2701 gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt
     2761 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     2821 tccagcctcc gcggccccga attcaccggt tctagagcta gcttcgaaga attcgatatc
     2881 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2941 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3001 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3061 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3121 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     3181 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3241 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     3301 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     3361 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     3421 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     3481 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     3541 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     3601 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     3661 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     3721 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     3781 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     3841 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     3901 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     3961 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     4021 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4081 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4141 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     4201 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4261 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4321 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4381 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4441 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4501 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4561 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4621 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4681 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4741 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4801 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4861 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4921 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4981 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5041 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5101 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5161 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     5221 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     5281 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     5341 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     5401 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     5461 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     5521 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     5581 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     5641 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     5701 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     5761 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     5821 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     5881 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5941 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6001 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6061 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6121 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6181 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6241 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6301 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6361 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6421 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6481 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6541 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6601 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6661 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6721 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6781 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6841 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6901 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6961 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7021 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7081 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7141 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7201 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7261 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7321 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7381 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7441 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7501 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7561 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7621 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7681 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7741 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7801 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7861 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7921 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7981 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8041 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8101 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8161 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8221 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8281 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     8341 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     8401 tgcgctgctt cg
//
