LOCUS       pcDNA3.1_NHAGFPO        6233 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 13, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    28..80
                     /label=bom (fragment)
                     /note="color: #a6acb3"
     misc_feature    235..614
                     /label=CMV enhancer
                     /note="color: #a6acb3"
     misc_feature    615..818
                     /label=CMV promoter
                     /note="color: #a6acb3"
     primer_bind     769..789
                     /label=CMV_F
                     /note="color: black; sequence: cgcaaatgggcggtaggcgtg"
     misc_feature    922..925
                     /label=Kozak
                     /note="color: #ffff00"
     CDS             926..1795
                     /codon_start=1
                     /label=NHAGFPOPT
                     /note="color: #b4abac"
                     /translation="MDAQTRRRERRAEKQAQWKAANGGSPPHMAYPYDVPDYAPPSLNS
                     ADIQHSGGRVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT
                     GKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTR
                     AEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIR
                     HNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA
                     GITLGMDE"
     misc_feature    926..1042
                     /label=NHA
                     /note="color: #75c6a9"
     CDS             926..1042
                     /label=Translation 926-1042
                     /note="color: #993366; direction: RIGHT"
     misc_feature    926..1042
                     /label=NHA
                     /note="color: #808000"
     misc_feature    1088..1795
                     /label=GFP Optimal
                     /note="color: #b1ff67"
     primer_bind     1147..1166
                     /label=GFPOPT_qPCR_F
                     /note="color: black; sequence: tgacgtgaacggtcacaaat"
     primer_bind     complement(1344..1363)
                     /label=GFPOPT_qPCR_R
                     /note="color: black; sequence: gtagccctctggcatagctg"
     primer_bind     1805..1823
                     /label=3' UTR qPCR F
                     /note="color: black; sequence: CCCGTTTAAACCCGCTGAT"
     primer_bind     complement(1827..1844)
                     /label=BGHR
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGG"
     primer_bind     complement(1896..1912)
                     /label=3' UTR qPCR R
                     /note="color: black; sequence: AGTGGGAGTGGCACCTT"
     misc_feature    1923..1928
                     /label=AATAAA
                     /note="color: #ff9ccd"
     misc_feature    2042..2057
                     /label=end of BGH PA term
                     /note="color: #c6c9d1"
     misc_feature    2103..2531
                     /label=f1 ori
                     /note="color: #00ffff"
     misc_feature    2545..2874
                     /label=SV40 promoter
                     /note="color: #ffffff"
     misc_feature    2941..3732
                     /label=neo
                     /note="color: #993366"
     misc_feature    3756..3914
                     /label=ble (fragment)
                     /note="color: #a6acb3"
     misc_feature    3909..4030
                     /label=SV40 pA signal
                     /note="color: #a6acb3"
     misc_feature    4103..4119
                     /label=lac operator
                     /note="color: #33cccc"
     misc_feature    4127..4157
                     /label=lac promoter
                     /note="color: #a6acb3"
     misc_feature    4172..4193
                     /label=CAP binding site
                     /note="color: #a6acb3"
     misc_feature    4206..4298
                     /label=lacI fragment
                     /note="color: #a6acb3"
     misc_feature    5237..6097
                     /label=Amp R
                     /note="color: #008080"
     misc_feature    6098..6202
                     /label=Amp R promoter
                     /note="color: #a6acb3"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccaacatgga cgcacaaaca cgacgacgtg agcgtcgcgc
      961 tgagaaacaa gctcaatgga aagctgcaaa cggtggatct cctccacata tggcttaccc
     1021 atacgatgtt ccagattacg ctcctccaag cttgaattct gcagatatcc agcacagtgg
     1081 cggccgcgtg agtaaaggcg aggagctgtt cactggcgtg gttccaattc tggttgagct
     1141 ggacggtgac gtgaacggtc acaaattctc agttagcggc gagggtgagg gcgacgctac
     1201 ctacggtaaa ctgaccttga aattcatttg cactactggt aaattaccag ttccatggcc
     1261 aactttagtt actactttga cctacggtgt tcagtgcttc agtcggtacc cggaccacat
     1321 gaaacagcac gacttcttca aatcagctat gccagagggc tacgtgcagg agcgtaccat
     1381 tttcttcaaa gacgacggta actacaaaac ccgagctgag gttaaattcg agggcgacac
     1441 cttggttaac cgtattgagt tgaaaggtat tgacttcaaa gaggacggca acattttggg
     1501 ccacaaatta gagtacaact acaacagcca caacgtttac attatggctg acaaacagaa
     1561 aaacggcatt aaagtgaact tcaaaattcg gcacaacatt gaggacggca gcgttcagct
     1621 ggccgaccac taccagcaga acaccccaat tggtgacggt ccagtgctgt tgccggacaa
     1681 ccactacctg agcactcaga gcgccttaag taaagaccca aacgagaaac gtgaccacat
     1741 ggtgttattg gagttcgtga ctgctgctgg cattaccctg ggcatggacg agtaatctag
     1801 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     1861 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     1921 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     1981 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     2041 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     2101 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     2161 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     2221 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     2281 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     2341 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     2401 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     2461 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     2521 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     2581 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     2641 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2701 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2761 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     2821 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     2881 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     2941 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3001 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3061 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3121 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3181 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     3241 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     3301 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     3361 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     3421 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     3481 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     3541 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     3601 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     3661 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     3721 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     3781 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     3841 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     3901 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3961 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4021 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     4081 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4141 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4201 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4261 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4321 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4381 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4441 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4501 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4561 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4621 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4681 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4741 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4801 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4861 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4921 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4981 gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga
     5041 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5101 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5161 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5221 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5281 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5341 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5401 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5461 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5521 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5581 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5641 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5701 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5761 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5821 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5881 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5941 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6001 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6061 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6121 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6181 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
