LOCUS       pcDNA3.1_SMFLuc1        8037 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Chloe Barrington-Ham
  TITLE     Direct Submission
  JOURNAL   Exported Oct 13, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8037
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        complement(100..204)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    263..315
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3"
     enhancer        470..849
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
                     /note="color: #a6acb3; direction: RIGHT"
     promoter        850..1053
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             1049..1082
                     /label=5' LTR (truncated) (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=18.8"
                     /note="/other=LTR"
                     /note="color: #ffe4c4; direction: RIGHT"
     promoter        1098..1116
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        1136
                     /label=UBC_human promoter
                     /note="color: #ffef86"
     CDS             1148..2157
                     /label=SpaghettiMonster_actb
                     /note="color: #c6c9d1; direction: RIGHT"
     CDS             1148..2122
                     /label=mRuby2_FLAG
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1151..1174
                     /label=FLAG
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1178..1201
                     /label=FLAG
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1205..1228
                     /label=FLAG
                     /note="color: #ff9ccd; direction: RIGHT"
     misc_feature    1418..1426
                     /label=dark chromophore: to make it bright, turn GGG into
                     /note="color: #ff9ccd"
     primer_bind     1664..1685
                     /label=SM_mid_F
                     /note="color: black; sequence: GAAATGATGTACCCCGCAGACG"
     CDS             1733..1756
                     /label=FLAG
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1763..1786
                     /label=FLAG
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1799..1822
                     /label=FLAG
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1829..1852
                     /label=FLAG
                     /note="color: #ff9ccd; direction: RIGHT"
     primer_bind     1964..1989
                     /label=SM_end_F
                     /note="color: black; sequence: GAAGAATCAGACAATGAGATGTTCGT"
     CDS             2045..2068
                     /label=FLAG
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             2072..2095
                     /label=FLAG
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             2099..2122
                     /label=FLAG
                     /note="color: #ff9ccd; direction: RIGHT"
     primer_bind     2123..2143
                     /label=annealing F
                     /note="color: black; sequence: GGAGGCGGTGGATCAGGTGGA"
     CDS             2158
                     /label=SpaghettiMonster_actb
                     /note="color: #c6c9d1; direction: RIGHT"
     misc_feature    2159..3808
                     /label=FLuc100
                     /note="color: #faac61"
     primer_bind     complement(2234..2261)
                     /label=FLuc100_begin_R
                     /note="color: black; sequence: 
                     ctagtgcgtatcgtttcatagctttgtg"
     primer_bind     complement(2848..2872)
                     /label=halfway_thru_FLuc100_R
                     /note="color: black; sequence: GCTTAAAATGGCAGTGTCCGGAATG"
     primer_bind     3163..3182
                     /label=qPCR_FLuc100_1F
                     /note="color: black; sequence: ccgacagggttacggtttga"
     primer_bind     3328..3347
                     /label=qPCR_FLuc100_2F_seq
                     /note="color: black; sequence: cgtgcgaggtccgatgatta"
     primer_bind     complement(3328..3347)
                     /label=qPCR_FLuc100_1R
                     /note="color: black; sequence: taatcatcggacctcgcacg"
     primer_bind     complement(3451..3474)
                     /label=mostly_thru_FLuc100_R
                     /note="color: black; sequence: GATTTCAGCCGGTCAACGATGAAG"
     primer_bind     complement(3456..3475)
                     /label=qPCR_FLuc100_2R
                     /note="color: black; sequence: tgatttcagccggtcaacga"
     primer_bind     3724..3749
                     /label=end_of_F100_F
                     /note="color: black; sequence: gaaaggtttaactggcaaattggacg"
     misc_feature    3789..3808
                     /label=4.20_anneal
                     /note="color: #ff9ccd"
     primer_bind     3900..3943
                     /label=malat1_gib_F
                     /note="color: black; sequence: 
                     aacccgctgatcagcctcgacgattcgtcagtagggttgtaaag; added: 
                     2022-06-10"
     primer_bind     complement(3903..3939)
                     /label=malat1_gib_V_R
                     /note="color: black; sequence: 
                     acaaccctactgacgaatcgtcgaggctgatcagcgg; added: 2022-06-10"
     misc_feature    3920..4093
                     /label=malat1
                     /note="color: #a6acb3"
     polyA_signal    3920
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(4025..4043)
                     /label=malat1_colonyPCR_R
                     /note="color: black; sequence: cagccaccagcgtcttttg; added: 
                     2022-07-01"
     ncRNA           4029..4085
                     /label=mascRNA-menRNA
                     /note="pLannotate"
                     /note="/database=Rfam"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=101.8"
                     /note="/other=ncRNA"
                     /note="color: #8fbc8f; direction: RIGHT"
     primer_bind     4074..4118
                     /label=malat1_gib_V_F
                     /note="color: black; sequence: 
                     tccctgcggtgtctttgcttcttctgaggcggaaagaaccagctg; added: 
                     2022-06-10"
     primer_bind     complement(4076..4113)
                     /label=malat1_gib_R
                     /note="color: black; sequence: 
                     ggttctttccgcctcagaagaagcaaagacaccgcagg; added: 2022-06-10"
     promoter        4097..4118
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4139..4567
                     /direction=RIGHT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     primer_bind     complement(4285..4307)
                     /label=f1_ori_sequencing_R
                     /note="color: black; sequence: cctaaagggagcccccgatttag; 
                     added: 2022-06-10"
     promoter        4581..4910
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4977..5768
                     /codon_start=1
                     /label=neo
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5792..5950
                     /codon_start=1
                     /label=ble (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=42.1"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLML
                     EFFAHPNL"
     polyA_signal    5945..6066
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    6139..6155
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     promoter        6163..6193
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     protein_bind    6208..6229
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
                     /note="color: #31849b"
     CDS             complement(6242..6334)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      6517..7105
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     CDS             complement(join(7276..8037,1..99))
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
       61 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
      121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
      181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
      241 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
      301 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
      361 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
      421 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      481 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      541 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      601 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      661 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      721 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      781 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      841 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      901 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      961 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1021 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
     1081 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgcggc
     1141 cgccaccatg gactacaagg acgacgacga caaaggtgac tacaaagatg atgacgataa
     1201 aggcgactat aaggacgatg acgacaaggg cggaaactca ctgatcaagg aaaacatgcg
     1261 gatgaaggtg gtgatggagg gctccgtgaa tggtcaccag ttcaagtgca ccggagaggg
     1321 agagggaaac ccgtacatgg gaactcagac catgcgcatt aaggtcatcg aaggaggtcc
     1381 gctgccgttc gctttcgata tcctggccac ttcgttcgga ggagggtcgc gcacgttcat
     1441 caagtacccg aagggaatcc cggacttctt taagcagtca ttcccggaag gattcacttg
     1501 ggaacgggtg acccggtatg aagatggagg tgtggtgact gtcatgcaag atacttcgct
     1561 ggaggatggg tgcctcgtgt accacgtcca agtccgcgga gtgaatttcc cgtccaacgg
     1621 accagtgatg cagaaaaaga cgaagggttg ggaacctaat actgaaatga tgtaccccgc
     1681 agacggaggg ctgaggggct acacccacat ggcgctgaag gtcgacggag gagattacaa
     1741 ggatgacgac gataagcaac aagattacaa agacgatgat gacaagggcc agcagggcga
     1801 ctacaaggac gacgacgaca agcagcagga ctacaaagat gacgatgata aaggaggagg
     1861 acatctgtcc tgttcgttcg tgaccaccta cagatcaaag aaaaccgtgg gaaacatcaa
     1921 gatgccgggc attcatgccg tcgaccaccg cctggagcgg ctcgaagaat cagacaatga
     1981 gatgttcgtc gtgcaaagag aacatgccgt ggccaagttc gcgggactgg gaggcggtgg
     2041 aggcgattac aaagacgatg atgacaaggg tgactataaa gacgacgatg acaaagggga
     2101 ttacaaggat gatgatgata agggaggcgg tggatcaggt ggaggaggtt cactgcagga
     2161 ggacgccaaa aacattaaaa aaggcccggc tccattctac ccgctggagg acggtactgc
     2221 tggcgagcag ctgcacaaag ctatgaaacg atacgcacta gttccgggta ctattgcttt
     2281 cactgacgct cacatcgagg tggacatcac ttacgctgag tacttcgaga tgagcgttcg
     2341 gttggccgag gctatgaaac gatacggcct gaacactaac caccgaatcg tggtgtgctc
     2401 agagaactca ttacagttct tcatgccggt gttgggcgcc ttattcatcg gcgttgctgt
     2461 tgccccagcc aacgacattt acaacgagcg agagttgtta aactcaatgg gcatttcaca
     2521 gccaactgtg gtgttcgttt caaaaaaagg cttgcagaaa attttgaacg tgcagaaaaa
     2581 attaccaatc atccagaaaa ttattatcat ggacagcaaa actgactacc agggtttcca
     2641 gagcatgtac actttcgtta cttcacactt gccaccgggt ttcaacgagt acgacttcgt
     2701 gccagagagc ttcgaccgag acaaaactat tgccctgatc atgaactcat caggctcaac
     2761 tggtctgccg aaaggtgttg ctctgccgca ccggactgcc tgcgtgcgat tctcacacgc
     2821 ccgggaccca attttcggca accagatcat tccggacact gccattttaa gcgttgttcc
     2881 attccaccac ggtttcggca tgttcactac tctgggttac ttcatatgcg gtttccgagt
     2941 tgtgttaatg taccggttcg aggaggagct gttcctgcga agcttgcagg actacaaaat
     3001 tcagtcagct ctgctggtgc caactctgtt cagcttcttc gccaaaagca ctctgattga
     3061 caaatacgac ttatcaaact tacacgagat tgcttcaggt ggcgctccgt tatcaaaaga
     3121 ggttggcgag gccgttgcca aacgattcca cctgccaggt atccgacagg gttacggttt
     3181 gactgagact acttcagcta ttctgattac tccggagggt gacgacaaac cgggcgctgt
     3241 gggtaaagtt gttccattct tcgaggctaa agttgtggac ctggacactg gtaaaactct
     3301 gggcgttaac cagcgaggcg agctgtgcgt gcgaggtccg atgattatga gcggttacgt
     3361 gaacaacccg gaggctacta acgccttgat tgacaaagac ggttggttac acagcggtga
     3421 catcgcttac tgggacgagg acgagcactt cttcatcgtt gaccggctga aatcactgat
     3481 taaatacaaa ggctaccagg tggctccggc tgagttggag tcaatcttgt tacagcaccc
     3541 aaacatcttc gacgctggtg tggctggtct gccggacgac gacgccggtg agttaccagc
     3601 cgccgttgtt gttttggagc acggtaaaac tatgactgag aaagagatcg tggactacgt
     3661 tgccagccag gttactactg ctaaaaaatt gcggggtggc gttgtgttcg tcgacgaggt
     3721 tccgaaaggt ttaactggca aattggacgc tcgaaaaatc cgagagatcc tgatcaaagc
     3781 caaaaaaggc ggcaaaatcg ccgtgtaagg taccgagctc ggatccacta gtccagtgtg
     3841 gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
     3901 acccgctgat cagcctcgac gattcgtcag tagggttgta aaggtttttc ttttcctgag
     3961 aaaacaacct tttgttttct caggttttgc tttttggcct ttccctagct ttaaaaaaaa
     4021 aaagcaaaag acgctggtgg ctggcactcc tggtttccag gacggggttc aagtccctgc
     4081 ggtgtctttg cttcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4141 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4201 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4261 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4321 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4381 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4441 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4501 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4561 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4621 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4681 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4741 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4801 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4861 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4921 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     4981 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5041 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5101 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5161 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5221 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5281 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5341 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5401 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5461 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     5521 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     5581 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     5641 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     5701 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     5761 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     5821 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5881 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5941 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6001 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6061 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6121 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6181 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6241 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6301 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     6961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagc
//
