LOCUS       20220610_pcDNA3.        8088 bp DNA     circular SYN 05-OCT-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 13, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(100..204)
                     /label=AmpR promoter
                     /note="label: AmpR promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    263..315
                     /label=bom (fragment)
                     /note="label: bom (fragment) note: pLannotate note: 
                     /database=snapgene note: /fragment=True note: 
                     /identity=100.0 note: /match_length=37.6 note: 
                     /other=misc_feature"
                     /note="color: #b4abac"
     enhancer        470..849
                     /label=CMV enhancer
                     /note="label: CMV enhancer note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=enhancer"
                     /note="color: #b4abac"
     promoter        850..1053
                     /label=CMV promoter
                     /note="label: CMV promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=promoter"
                     /note="color: #c6c9d1"
     LTR             1049..1082
                     /label=5' LTR (truncated) (fragment)
                     /note="label: 5' LTR (truncated) (fragment) note: 
                     pLannotate note: /database=snapgene note: /fragment=True 
                     note: /identity=97.1 note: /match_length=18.8 note: 
                     /other=LTR"
                     /note="color: #f8d3a9"
     promoter        1098..1116
                     /label=T7 promoter
                     /note="label: T7 promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=promoter"
                     /note="color: #c6c9d1"
     promoter        1136
                     /label=UBC_human promoter
                     /note="label: UBC_human promoter"
                     /note="color: #ffef86"
     CDS             1148..2157
                     /label=SpaghettiMonster_actb
                     /note="label: SpaghettiMonster_actb"
                     /note="color: #c6c9d1; direction: RIGHT"
     CDS             1148..2122
                     /label=mRuby2_FLAG
                     /note="label: mRuby2_FLAG"
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1151..1174
                     /label=FLAG
                     /note="label: FLAG"
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1178..1201
                     /label=FLAG
                     /note="label: FLAG"
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1205..1228
                     /label=FLAG
                     /note="label: FLAG"
                     /note="color: #ff9ccd; direction: RIGHT"
     misc_feature    1418..1426
                     /label=dark chromophore: to make it bright, turn GGG into
                     /note="label: dark chromophore: to make it bright, turn GGG
                     into"
                     /note="color: #ff9ccd"
     CDS             1733..1756
                     /label=FLAG
                     /note="label: FLAG"
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1763..1786
                     /label=FLAG
                     /note="label: FLAG"
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1799..1822
                     /label=FLAG
                     /note="label: FLAG"
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             1829..1852
                     /label=FLAG
                     /note="label: FLAG"
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             2045..2068
                     /label=FLAG
                     /note="label: FLAG"
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             2072..2095
                     /label=FLAG
                     /note="label: FLAG"
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             2099..2122
                     /label=FLAG
                     /note="label: FLAG"
                     /note="color: #ff9ccd; direction: RIGHT"
     CDS             2158
                     /label=SpaghettiMonster_actb
                     /note="label: SpaghettiMonster_actb"
                     /note="color: #c6c9d1; direction: RIGHT"
     misc_feature    2159..3808
                     /label=Firefly Luciferase 0%
                     /note="label: Firefly Luciferase 0%"
                     /note="color: #ffef86"
     misc_feature    3779..3805
                     /label=4.20_anneal_F0
                     /note="label: 4.20_anneal_F0"
                     /note="color: #ff9ccd"
     polyA_signal    3920..4144
                     /label=bGH poly(A) signal
                     /note="label: bGH poly(A) signal note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=polyA_signal"
                     /note="color: #b4abac"
     promoter        4148..4169
                     /label=SV40 promoter (fragment)
                     /note="label: SV40 promoter (fragment) note: pLannotate 
                     note: /database=snapgene note: /fragment=True note: 
                     /identity=100.0 note: /match_length=6.1 note: 
                     /other=promoter"
                     /note="color: #c6c9d1"
     rep_origin      4190..4618
                     /label=f1 ori
                     /note="direction: RIGHT label: f1 ori note: pLannotate 
                     note: /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=rep_origin"
                     /note="color: #ffef86"
     promoter        4632..4961
                     /label=SV40 promoter
                     /note="label: SV40 promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=99.7 note: /match_length=100.0 note: 
                     /other=promoter"
                     /note="color: #c6c9d1"
     CDS             5028..5819
                     /label=neo
                     /note="codon_start: 1 label: neo note: pLannotate note: 
                     /database=swissprot note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: /other=CDS 
                     transl_table: 1 translation: 
                     MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNE
                     LQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMAD
                     AMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKAR
                     MPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEW
                     ADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /note="color: #993366; direction: RIGHT"
     CDS             5843..6001
                     /label=ble (fragment)
                     /note="codon_start: 1 label: ble (fragment) note: 
                     pLannotate note: /database=swissprot note: /fragment=True 
                     note: /identity=98.1 note: /match_length=42.1 note: 
                     /other=CDS transl_table: 1 translation: 
                     MTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLMLEFFAHPNL"
                     /note="color: #993366; direction: RIGHT"
     polyA_signal    5996..6117
                     /label=SV40 poly(A) signal
                     /note="label: SV40 poly(A) signal note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=polyA_signal"
                     /note="color: #b4abac"
     protein_bind    6190..6206
                     /label=lac operator
                     /note="label: lac operator note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=protein_bind"
                     /note="color: #31849b"
     promoter        6214..6244
                     /label=lac promoter
                     /note="label: lac promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=promoter"
                     /note="color: #c6c9d1"
     protein_bind    6259..6280
                     /label=CAP binding site
                     /note="label: CAP binding site note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=protein_bind"
                     /note="color: #31849b"
     CDS             complement(6293..6385)
                     /label=lacI (fragment)
                     /note="codon_start: 1 label: lacI (fragment) note: 
                     pLannotate note: /database=snapgene note: /fragment=True 
                     note: /identity=100.0 note: /match_length=8.6 note: 
                     /other=CDS transl_table: 1 translation: 
                     APNTQTASPRALADSLMQLARQVSRLESGQ*"
                     /note="color: #993366; direction: LEFT"
     rep_origin      6568..7156
                     /label=ori
                     /note="label: ori note: pLannotate note: /database=snapgene
                     note: /fragment=False note: /identity=99.8 note: 
                     /match_length=100.0 note: /other=rep_origin"
                     /note="color: #ffef86"
     CDS             complement(join(7327..8088,1..99))
                     /label=AmpR
                     /note="codon_start: 1 label: AmpR note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=99.8 note: /match_length=100.0 note: /other=CDS 
                     transl_table: 1 translation: 
                     MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFR
                     PEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVR
                     ELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERD
                     TTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAG
                     ERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /note="color: #b7e6d7; direction: LEFT"
ORIGIN
        1 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
       61 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
      121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
      181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
      241 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
      301 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
      361 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
      421 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      481 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      541 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      601 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      661 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      721 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      781 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      841 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      901 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      961 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1021 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
     1081 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgcggc
     1141 cgccaccatg gactacaagg acgacgacga caaaggtgac tacaaagatg atgacgataa
     1201 aggcgactat aaggacgatg acgacaaggg cggaaactca ctgatcaagg aaaacatgcg
     1261 gatgaaggtg gtgatggagg gctccgtgaa tggtcaccag ttcaagtgca ccggagaggg
     1321 agagggaaac ccgtacatgg gaactcagac catgcgcatt aaggtcatcg aaggaggtcc
     1381 gctgccgttc gctttcgata tcctggccac ttcgttcgga ggagggtcgc gcacgttcat
     1441 caagtacccg aagggaatcc cggacttctt taagcagtca ttcccggaag gattcacttg
     1501 ggaacgggtg acccggtatg aagatggagg tgtggtgact gtcatgcaag atacttcgct
     1561 ggaggatggg tgcctcgtgt accacgtcca agtccgcgga gtgaatttcc cgtccaacgg
     1621 accagtgatg cagaaaaaga cgaagggttg ggaacctaat actgaaatga tgtaccccgc
     1681 agacggaggg ctgaggggct acacccacat ggcgctgaag gtcgacggag gagattacaa
     1741 ggatgacgac gataagcaac aagattacaa agacgatgat gacaagggcc agcagggcga
     1801 ctacaaggac gacgacgaca agcagcagga ctacaaagat gacgatgata aaggaggagg
     1861 acatctgtcc tgttcgttcg tgaccaccta cagatcaaag aaaaccgtgg gaaacatcaa
     1921 gatgccgggc attcatgccg tcgaccaccg cctggagcgg ctcgaagaat cagacaatga
     1981 gatgttcgtc gtgcaaagag aacatgccgt ggccaagttc gcgggactgg gaggcggtgg
     2041 aggcgattac aaagacgatg atgacaaggg tgactataaa gacgacgatg acaaagggga
     2101 ttacaaggat gatgatgata agggaggcgg tggatcaggt ggaggaggtt cactgcagga
     2161 agatgcgaag aatataaaga agggacccgc gcctttttat cccctagaag atggaaccgc
     2221 gggagaacaa ctacataagg caatgaagag atatgcacta gtacctggaa caatagcatt
     2281 tacagatgca catatagaag tcgatataac gtatgcggaa tattttgaaa tgtccgtccg
     2341 tctcgcagaa gcgatgaaga gatatgggct aaatacaaat catagaatag tcgtatgtag
     2401 tgaaaattct cttcaatttt ttatgcctgt cctcggggcg ctatttatag gagtcgcagt
     2461 cgcgcccgcg aatgatatat ataatgaacg tgaactcctc aatagtatgg gaatatcgca
     2521 acctaccgta gtatttgtat ccaagaaggg gctccaaaag atactcaatg tccaaaagaa
     2581 gctccccata atccaaaaga tcataataat ggattctaag acggattatc aaggatttca
     2641 atcgatgtat acgtttgtca catctcatct acctcccgga tttaatgaat atgattttgt
     2701 ccctgaatcc tttgataggg ataagacaat agcactaata atgaattcct ctggatctac
     2761 cggacttcct aagggggtcg cacttcctca tagaaccgca tgtgtcagat tttcgcatgc
     2821 gagagatcct atatttggca atcaaataat acctgataca gcgatactta gtgtcgtccc
     2881 ttttcatcat gggtttggaa tgtttactac actcggatat ttcatatgtg gatttcgcgt
     2941 cgtcctcatg tatagatttg aagaagaact ctttctaagg tcccttcaag attataagat
     3001 acaaagtgcg ctcctcgtcc ctaccctatt ctcctttttt gcaaagagta cccttataga
     3061 taagtatgat ctttctaatc tacatgaaat agcatctggg ggagcgcccc tctctaagga
     3121 agtcggggaa gcggtagcaa agaggtttca tctacctggg ataaggcaag gatatgggct
     3181 cacagaaacc acaagtgcga tactaataac acccgaaggg gatgataagc ctggagcggt
     3241 cgggaaggta gtaccctttt ttgaagcgaa ggtcgtagat cttgataccg ggaagacgct
     3301 tggagtcaat caaagagggg aactctgtgt cagaggacct atgataatgt ccggatatgt
     3361 aaataatccc gaagcgacca atgcgctaat agataaggat ggatggctac attctggaga
     3421 tatagcgtat tgggatgaag atgaacattt ttttatagtt gaccgcctaa agtctctcat
     3481 aaagtataag gggtatcaag tagcacccgc agaacttgaa tccatactac tccaacatcc
     3541 caatatattt gatgcagggg tcgcaggact tcccgatgat gatgcggggg aacttcccgc
     3601 agcggtcgtc gtcctcgaac atggaaagac gatgacggaa aaggaaatag tagattatgt
     3661 cgcaagtcaa gtaacaaccg cgaagaagct tcgcggagga gtcgtatttg tcgacgaagt
     3721 acccaagggg cttaccggaa agctcgatgc aagaaagata agagaaatac tcataaaggc
     3781 aaagaagggg ggaaagatag cagtataagg taccgagctc ggatccacta gtccagtgtg
     3841 gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
     3901 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3961 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4021 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4081 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4141 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     4201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4261 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4321 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4381 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4441 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4501 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     4621 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4681 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4741 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4801 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4861 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     4921 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     4981 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5041 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5101 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5161 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     5221 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5281 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5341 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5401 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5461 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5521 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     5581 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5641 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5701 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5761 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5821 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     5881 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5941 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6001 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6061 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6121 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6181 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6241 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6301 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6361 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6421 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6481 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6541 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6601 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6661 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6721 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6781 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6841 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6901 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6961 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7021 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7081 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7141 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7201 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7261 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7321 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7381 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7441 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7501 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7561 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7621 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7681 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7741 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7801 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7861 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7921 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7981 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8041 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagc
//
